Sequence elements involved in the rescue of IBV defective RNA CD-91.
Adv Exp Med Biol
; 440: 253-7, 1998.
Article
en En
| MEDLINE
| ID: mdl-9782289
ABSTRACT
Deletion mutagenesis has been used to identify essential regions for rescue of coronavirus defective RNAs (D-RNAs). Using this technique on a cloned IBV D-RNA CD-91, we have identified a region potentially important in its rescue. Comparing the sequence of D-RNAs rescued with those not rescued we have deduced that a 72 base region corresponding to base number 13,824 to 13,896 in the viral genome is required for rescue. This may be an IBV D-RNA packaging signal or a cis-acting element involved in replication. Further experiments and modification of our techniques will be required to differentiate between the two processes.
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Bases de datos:
MEDLINE
Asunto principal:
ARN Viral
/
Virus de la Bronquitis Infecciosa
/
Virus Defectuosos
Límite:
Animals
Idioma:
En
Revista:
Adv Exp Med Biol
Año:
1998
Tipo del documento:
Article
País de afiliación:
Reino Unido