Cloning and characterization of the cDNA encoding the PH20 protein in the European red fox Vulpes vulpes.
Reprod Fertil Dev
; 10(2): 165-72, 1998.
Article
en En
| MEDLINE
| ID: mdl-9801268
ABSTRACT
The PH20 protein is thought to play a crucial role in mammalian fertilization. The fox PH20 homologue has been cloned from a testis cDNA library and the deduced protein sequence shows high levels of homology to PH20 proteins isolated from other species. Unlike other PH20 proteins the fox protein does not appear to be membrane associated through a GPI-linkage nor does it show the presence of a transmembrane domain at the C-terminus of the protein. It is in this region that the proteins appear to be least conserved. Immunolocalization studies on fox sperm show that the PH20 protein is located on the inner acrosomal membrane. Transcription of PH20 in the fox is seasonally regulated, with the mRNA expressed during those months when spermatogenesis is at its peak. The PH20 sequence described in this paper has been submitted to the Genbank database and has the accession number U41412.
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Bases de datos:
MEDLINE
Asunto principal:
Moléculas de Adhesión Celular
/
Anticoncepción Inmunológica
/
Zorros
/
Hialuronoglucosaminidasa
Límite:
Animals
Idioma:
En
Revista:
Reprod Fertil Dev
Asunto de la revista:
MEDICINA REPRODUTIVA
Año:
1998
Tipo del documento:
Article
País de afiliación:
Australia