Quantification of hepatitis A virus in shellfish by competitive reverse transcription-PCR with coextraction of standard RNA.
Appl Environ Microbiol
; 65(1): 322-6, 1999 Jan.
Article
en En
| MEDLINE
| ID: mdl-9872801
ABSTRACT
To quantify hepatitis A virus (HAV) in experimentally contaminated mussels, we developed an internal standard RNA with a 7-nucleotide deletion for competitive reverse transcription (RT)-PCR. Deposited directly into the sample, this standard was used both as extraction control and as quantification tool. After coextraction and competitive RT-PCR, standard and wild-type products were detected by differential hybridization with specific probes and a DNA enzyme immunoassay. The quantifiable range with this reproducible method was 10(4) to 10(7) copies of HAV/gram or 400 to 10(6) 50% tissue culture infective doses/ml.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Mariscos
/
ARN Viral
/
Bivalvos
/
Hepatovirus
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Appl Environ Microbiol
Año:
1999
Tipo del documento:
Article
País de afiliación:
Francia