Use of failed-fertilized oocytes for diagnostic zona binding purposes after sperm binding improvement with a modified medium.
J Assist Reprod Genet
; 16(1): 24-9, 1999 Jan.
Article
en En
| MEDLINE
| ID: mdl-9987690
ABSTRACT
PURPOSE:
Because the availability of prophase oocytes for zona binding testing is limited, we compared sperm binding to the zona of failed-fertilized intracytoplasmic sperm injection (ICSI) and in vitro fertilization (IVF) oocytes after incubation in a standard IVF medium and a specially composed binding improvement medium.METHODS:
Semen samples from nine patients and nine fertile donors were separated in parallel by the standard swim-up method in both media. Subsequently, hemizona assays were performed with prophase, failed-fertilized ICSI and IVF oocytes.RESULTS:
Sperm separation resulted in a significantly higher sperm count (P < 0.01) and progressive motility (P = 0.018) in binding improvement medium. Moreover, spermatozoa coincubated with hemizonae (prophase, failed-fertilized ICSI and IVF oocytes) in binding improvement medium bound significantly more to hemizonae than in the controls (P < 0.01). However, the hemizona index did not differ.CONCLUSIONS:
Thus, the limited number of human zonae can be increased by the use of oocytes that failed to fertilize during ICSI or IVF. This will lead to a qualitative improvement of the diagnostic spectrum in male-factor infertility.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Interacciones Espermatozoide-Óvulo
/
Espermatozoides
/
Zona Pelúcida
/
Técnicas Reproductivas
/
Infertilidad Masculina
Tipo de estudio:
Diagnostic_studies
/
Qualitative_research
Límite:
Adolescent
/
Adult
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
J Assist Reprod Genet
Asunto de la revista:
GENETICA
/
MEDICINA REPRODUTIVA
Año:
1999
Tipo del documento:
Article
País de afiliación:
Alemania