Integrating Anisakis spp. parasites data and host genetic structure in the frame of a holistic approach for stock identification of selected Mediterranean Sea fish species.

The unique environment of the Mediterranean Sea makes fish stock assessment a major challenge. Stock identification of Mediterranean fisheries has been based mostly from data on biology, morphometrics, artificial tags, otolith shape and fish genetics, with less effort on the use of parasites as biomarkers. Here we use some case studies comparing Mediterranean vs Atlantic fish stocks in a multidisciplinary framework. The generalized Procrustes Rotation (PR) was used to assess the association between host genetics and larval Anisakis spp. datasets on demersal (hake) and pelagic (horse mackerel, swordfish) species. When discordant results emerged, they were due to the different features of the data. While fish population genetics can detect changes over an evolutionary timescale, providing indications on the cohesive action of gene flow, parasites are more suitable biomarkers when considering fish stocks over smaller temporal and spatial scales, hence giving information of fish movements over their lifespan. Future studies on the phylogeographic analysis of parasites suitable as biomarkers, and that of their fish host, performed on the same genes, will represent a further tool to be included in multidisciplinary studies on fish stock structure.

Distribution of different species of metacercariae in two freshwater fishes: Haludaria fasciata (Teleostei: Cyprinidae) and Pseudosphromenus cupanus (Teleostei: Osphromenidae).

Information on the distribution and abundance patterns of trematodes are essential to reveal the ecology of host-parasite interactions. The Western Ghats of India, a biodiversity hotspot, is rich in freshwater fish diversity and endemism. Though there are several studies on various other aspects of fish ecology, studies on their parasitic fauna is meager. The objective of the present study is to explore the distribution and infection patterns of metacercariae of five species of trematodes in the freshwater fishes, Haludaria fasciata and Pseudosphromenus cupanus. The infection parameters were analyzed for each host and CART model was applied to analyze the environmental factors affecting parasite distribution patterns. All species of metacercariae showed an over-dispersed aggregate distributions. The classification tree models indicated that among the environmental factors considered, differences in host locality was the most influential factor in both fishes, followed at a greater distance by the factor seasonality. The parasite communities exhibited temporal and spatial differences in the infection pattern in response to seasonal and locational variations.

Tetracotyle wayanadensis (Trematoda: Digenea) metacercaria - A potential parasitic castrator of the freshwater fish Aplocheilus lineatus (Valenciennes, 1846): A histopathological and temporal variation study in the South Western Ghats, India.

Parasitic castration is a phenomenon where the parasite blocks or reduces the host reproductive output. This study explored the impact of Tetracotyle wayanadensis infection on the reproductive performance of the freshwater fish Aplocheilus lineatus. Heavily infected A. lineatus showed slow and sluggish movements with reduced food intake and feeding activities. Histopathological observations of the ovary revealed severe necrosis and degeneration of previtellogenic and vitellogenic oocytes and follicular atresia. The oogonial nests were absent; chromatin nucleolar oocytes irregularly shaped and vacuolated. Perinucleolar oocytes have a vacuolated appearance with the hypertrophied highly columnar follicular cells invading them. Vitellogenic oocytes had a shrunken appearance with folded zona radiata; yolk globules broken down into smaller granules together with vacuolar degeneration of the ooplasm. The hypertrophied highly columnar follicular cells invaded the early vitellogenic oocytes to phagocytize the degenerating material. Zona radiata was found broken and liquefaction of yolk globules was evident with advancement in follicular atresia. Seasonal variation of parasitic infection has also been studied. Metacercaria infecting the vital organs showed seasonal variation with the highest values for prevalence and abundance during monsoon and mean intensity during winter. The lowest values for these descriptors were documented during the post-monsoon period.

Anxious adults vs. cool children: children's views on smoking and addiction.

Tobacco addiction represents a major public health problem, and most addicted smokers take up the habit during adolescence. We need to know why. With the aim of gaining a better understanding of the meanings smoking and tobacco addiction hold for young people, 85 focused interviews were conducted with adolescent children from economically deprived areas of Northern Ireland. Through adopting a qualitative approach within the community rather than the school context, the adolescent children were given the opportunity to freely express their views in confidence. Children seem to differentiate conceptually between child smoking and adult smoking. Whereas adults smoke to cope with life and are thus perceived by children as lacking control over their consumption, child smoking is motivated by attempts to achieve the status of cool and hard, and to gain group membership. Adults have personal reasons for smoking, while child smoking is profoundly social. Adults are perceived as dependent on nicotine, and addiction is at the core of the children's understanding of adult smoking. Child smoking, on the other hand, is seen as oriented around social relations so that addiction is less relevant. These ideas leave young people vulnerable to nicotine addiction. It is clearly important that health promotion efforts seek to understand and take into account the actions of children within the context of their own world-view to secure their health.

Distribution of Anisakis larvae, identified by genetic markers, and their use for stock characterization of demersal and pelagic fish from European waters: an update.

In the present paper, recent results obtained on the use of different distributions observed in larval species of Anisakis, genetically identified by means of allozyme markers, for stock characterization of demersal (Merluccius merluccius), small (Trachurus trachurus) and large pelagic (Xiphias gladius) finfish species in European waters, are reviewed and discussed. Several species of Anisakis were identified in the three fish hosts: A. simplex (s.s.), A. physeteris, A. typica, A. ziphidarum, A. pegreffii, A. brevispiculata and A. paggiae. Canonical discriminant analysis performed on all the samples of the three fish species collected in areas comprising their geographical range, according to the different species of Anisakis identified, showed distinct fish populations in European waters. In all the three fish hosts, the pattern of distribution of Anisakis larvae allowed discrimination of Mediterranean stocks from Atlantic stocks. In the case of swordfish, the possible existence of a southern Atlantic stock separated from a northern one is also suggested. Congruence and discordance with the population genetic data inferred from allozyme markers on the same samples of the three fish species are also discussed.

Alcohol consumption among 11-16 year olds: 'getting around' structural barriers?

This paper presents qualitative data from Irish children and adolescents on their experiences in relation to alcohol consumption. A sample of 78 participants (average age 11.5 years) was selected. A proportion of this initial sample were interviewed at intervals over a period of 3 years. The participants' consumption patterns were analyzed and four categories were generated: covert unsanctioned, overt unsanctioned, overt sanctioned, and peer unsanctioned. As the children got older, peer drinking became a stronger feature of the data; however, it mediated other patterns of behavior. Although the children displayed agency in circumventing adult rules relating to alcohol consumption, the participants were subjected to structural constraints by virtue of their status as children. Moreover, the agentic powers of the participants were procured through their social network rather than arising from an essentialist agency possessed by each individual child. The impact of childhood as a structural dimension weakened to some extent as the participants got older and had more freedom to circumvent adult-defined barriers to alcohol consumption.

Smoking and symbolism: children, communication and cigarettes.

Health promotion, with its concern with empowerment and autonomy, must recognize the agency of its target population. Based on 85 in-depth interviews with 10- to 11-year-old children throughout Northern Ireland, this paper argues that it is necessary to focus on the social relations of children if we are to understand and prevent childhood smoking. Addressing the complex issue of childhood agency, it is argued that regardless of various restrictions to their choices, children can act intentionally in constructing their identities. Instead of viewing the smoking children as communicating with the adult world, we focus on smoking as negotiation of status within the children's culture. Such negotiations utilize symbolism derived from and shared with the 'adult world'. It is important that those analyzing children's lives understand children's ideas and behaviour on their own terms. We must make sure that the very concepts in which the children's experiences are put are appropriate ones. It is suggested that the metaphor 'rite of passage' and terminology such as peer 'pressure' versus adult 'influence', commonly used to analyse the children's smoking behaviour, may actually conceal important aspects of childhood agency.