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1.
Chemistry ; 30(52): e202401998, 2024 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-38962903

RESUMO

The capability of amyloid-like peptide fibers to emit intrinsic-fluorescence enables the study of their formation, stability and hardening through time-resolved fluorescence analysis, without the need for additional intercalating dyes. This approach allows the monitoring of amyloid-like peptides aggregation kinetics using minimal sample volumes, and the simultaneous testing of numerous experimental conditions and analytes, offering rapid and reproducible results. The analytical procedure applied to the aromatic hexapeptide F6, alone or derivatized with PEG (polyethylene glycol) moiety of different lengths, suggests that aggregation into large anisotropic structures negatively correlates with initial monomer concentration and relies on the presence of charged N- and C-termini. PEGylation reduces the extent of aggregates hardening, possibly by retaining water, and overall impacts the final structural properties of the aggregates.


Assuntos
Amiloide , Peptídeos , Polietilenoglicóis , Cinética , Amiloide/química , Polietilenoglicóis/química , Peptídeos/química , Espectrometria de Fluorescência , Agregados Proteicos , Fluorescência
2.
Langmuir ; 40(2): 1470-1486, 2024 01 16.
Artigo em Inglês | MEDLINE | ID: mdl-38174846

RESUMO

Peptides are able to self-organize in structural elements including cross-ß structures. Taking advantage of this tendency, in the last decades, peptides have been scrutinized as molecular elements for the development of multivalent supramolecular architectures. In this context, different classes of peptides, also with completely aromatic sequences, were proposed. Our previous studies highlighted that the (FY)3 peptide, which alternates hydrophobic phenylalanine and more hydrophilic tyrosine residues, is able to self-assemble, thanks to the formation of both polar and apolar interfaces. It was observed that the replacement of Phe and Tyr residues with other noncoded aromatic amino acids like 2-naphthylalanine (Nal) and Dopa affects the interactions among peptides with consequences on the supramolecular organization. Herein, we have investigated the self-assembling behavior of two novel (FY)3 analogues with Trp and Dopa residues in place of the Phe and Tyr ones, respectively. Additionally, PEGylation of the N-terminus was analyzed too. The supramolecular organization, morphology, and capability to gel were evaluated using complementary techniques, including fluorescence, Fourier transform infrared spectroscopy, and scanning electron microscopy. Structural periodicities along and perpendicular to the fiber axis were detected by grazing incidence wide-angle X-ray scattering. Finally, molecular dynamics studies provided interesting insights into the atomic structure of the cross-ß that constitutes the basic motif of the assemblies formed by these novel peptide systems.


Assuntos
Triptofano , Tirosina , Tirosina/química , Triptofano/química , Di-Hidroxifenilalanina , Peptídeos/química , Aminoácidos Aromáticos/química
3.
J Pept Sci ; 30(7): e3573, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38471735

RESUMO

Advantages like biocompatibility, biodegradability and tunability allowed the exploitation of peptides and peptidomimetics as versatile therapeutic or diagnostic agents. Because of their selectivity towards transmembrane receptors or cell membranes, peptides have also been identified as suitable molecules able to deliver in vivo macromolecules, proteins or nucleic acids. However, after the identification of the homodimer diphenylalanine (FF) as an aggregative motif inside the Aß1-42 polypeptide, short and ultrashort peptides have been studied as building blocks for the fabrication of supramolecular, ordered nanostructures for applications in biotechnological, biomedical and industrial fields. In this perspective, many hybrid molecules that combine FF with other chemical entities have been synthesized and characterized. Two novel hybrid derivatives (tFaF and cFgF), in which the FF homodimer is alternated with the peptide-nucleic acid (PNA) heterodimer "g-c" (guanine-cytosine) or "a-t" (adenine-thymine) and their dimeric forms (tFaF)2 and (cFgF)2 were synthesized. The structural characterization performed by circular dichroism (CD), Fourier transform infrared (FTIR) and fluorescence spectroscopies highlighted the capability of all the FF-PNA derivatives to self-assemble into ß-sheet structures. As a consequence of this supramolecular organization, the resulting aggregates also exhibit optoelectronic properties already reported for other similar nanostructures. This photoemissive behavior is promising for their potential applications in bioimaging.


Assuntos
Ácidos Nucleicos Peptídicos , Ácidos Nucleicos Peptídicos/química , Ácidos Nucleicos Peptídicos/síntese química , Peptídeos/química , Peptídeos/síntese química , Fenilalanina/química , Fenilalanina/análogos & derivados , Dicroísmo Circular , Dipeptídeos/química , Dipeptídeos/síntese química
4.
J Pept Sci ; 30(10): e3607, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-38710638

RESUMO

There is an expanding body of evidence showing that synthetic peptides in combination with radioactive isotopes can be utilized for medical purposes. This area is of particular interest in oncology where applications in diagnosis and therapy are at different stages of development. We review the contributions in this area by the group originally founded by Carlo Pedone in Naples many years ago. We highlight the work of this group in the context of other developments in this area, focusing on three biologically relevant receptor systems: somatostatin, gastrin-releasing peptide, and cholecystokinin-2/gastrin receptors. We focus on key milestones, state of the art, and challenges in this area of research as well as the current and future outlook for expanding clinical applications.


Assuntos
Neoplasias , Peptídeos , Compostos Radiofarmacêuticos , Humanos , Neoplasias/tratamento farmacológico , Neoplasias/diagnóstico por imagem , Peptídeos/química , Peptídeos/uso terapêutico , Compostos Radiofarmacêuticos/química , Compostos Radiofarmacêuticos/uso terapêutico , Somatostatina/análogos & derivados , Somatostatina/uso terapêutico , Peptídeo Liberador de Gastrina/química , Peptídeo Liberador de Gastrina/metabolismo , Peptídeo Liberador de Gastrina/antagonistas & inibidores , Receptor de Colecistocinina B/metabolismo , Receptor de Colecistocinina B/antagonistas & inibidores , Radioisótopos/química , Radioisótopos/uso terapêutico , Animais , Terapia de Alvo Molecular/métodos
5.
Chemistry ; 29(28): e202300661, 2023 May 16.
Artigo em Inglês | MEDLINE | ID: mdl-36877530

RESUMO

Short and ultra-short peptides have been recently envisioned as excellent building blocks for the formulation of hydrogels with appealing properties. Due to its simplicity and capability to gel under physiological conditions, Fmoc-FF (Nα -fluorenylmethoxycarbonyl-diphenylalanine), remains one of the most studied low molecular-weight hydrogelators. Since its first identification in 2006, a plethora of its analogues were synthetized and investigated for the fabrication of novel supramolecular materials. Here we report a description of the Fmoc-FF analogues in which the aromatic Fmoc group is replaced with other substituents. These analogues are distinguished into five different classes including derivatives: i) customized with solid phase peptide synthesis protecting groups; ii) containing non-aromatic groups, iii) containing aromatic groups, iv) derivatized with metal complexes and v) containing stimuli-responsive groups. The morphological, mechanical, and functional effects caused by this modification on the resulting material are also pointed out.


Assuntos
Fluorenos , Peptídeos , Peptídeos/química , Fluorenos/química , Hidrogéis/química , Fenilalanina/química
6.
Biomacromolecules ; 24(1): 213-224, 2023 01 09.
Artigo em Inglês | MEDLINE | ID: mdl-36520063

RESUMO

The conformation and self-assembly of four lipopeptides, peptide amphiphiles comprising peptides conjugated to lipid chains, in aqueous solution have been examined. The peptide sequence in all four lipopeptides contains the integrin cell adhesion RGDS motif, and the cytocompatibility of the lipopeptides is also analyzed. Lipopeptides have either tetradecyl (C14, myristyl) or hexadecyl (C16, palmitoyl) lipid chains and peptide sequence WGGRGDS or GGGRGDS, that is, with either a tryptophan-containing WGG or triglycine GGG tripeptide spacer between the bioactive peptide motif and the alkyl chain. All four lipopeptides self-assemble above a critical aggregation concentration (CAC), determined through several comparative methods using circular dichroism (CD) and fluorescence. Spectroscopic methods [CD and Fourier transform infrared (FTIR) spectroscopy] show the presence of ß-sheet structures, consistent with the extended nanotape, helical ribbon, and nanotube structures observed by cryogenic transmission electron microscopy (cryo-TEM). The high-quality cryo-TEM images clearly show the coexistence of helically twisted ribbon and nanotube structures for C14-WGGRGDS, which highlight the mechanism of nanotube formation by the closure of the ribbons. Small-angle X-ray scattering shows that the nanotapes comprise highly interdigitated peptide bilayers, which are also present in the walls of the nanotubes. Hydrogel formation was observed at sufficiently high concentrations or could be induced by a heat/cool protocol at lower concentrations. Birefringence due to nematic phase formation was observed for several of the lipopeptides, along with spontaneous flow alignment of the lyotropic liquid crystal structure in capillaries. Cell viability assays were performed using both L929 fibroblasts and C2C12 myoblasts to examine the potential uses of the lipopeptides in tissue engineering, with a specific focus on application to cultured (lab-grown) meat, based on myoblast cytocompatibility. Indeed, significantly higher cytocompatibility of myoblasts was observed for all four lipopeptides compared to that for fibroblasts, in particular at a lipopeptide concentration below the CAC. Cytocompatibility could also be improved using hydrogels as cell supports for fibroblasts or myoblasts. Our work highlights that precision control of peptide sequences using bulky aromatic residues within "linker sequences" along with alkyl chain selection can be used to tune the self-assembled nanostructure. In addition, the RGDS-based lipopeptides show promise as materials for tissue engineering, especially those of muscle precursor cells.


Assuntos
Lipopeptídeos , Nanoestruturas , Lipopeptídeos/farmacologia , Lipopeptídeos/química , Adesão Celular , Sequência de Aminoácidos , Mioblastos , Dicroísmo Circular
7.
Soft Matter ; 19(25): 4686-4696, 2023 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-37313785

RESUMO

Short and ultra-short peptides have recently emerged as suitable building blocks for the fabrication of self-assembled innovative materials. Peptide aggregation is strictly related to the amino acids composing the sequence and their capability to establish intermolecular interactions. Additional structural and functional properties can also be achieved by peptide derivatization (e.g. with polymeric moieties, alkyl chains or other organic molecules). For instance, peptide amphiphiles (PAs), containing one or more alkyl tails on the backbone, have a propensity to form highly ordered nanostructures like nanotapes, twisted helices, nanotubes and cylindrical nanostructures. Further lateral interactions among peptides can also promote hydrogelation. Here we report the synthesis and the aggregation behaviour of four PAs containing cationic tetra- or hexa-peptides (C19-VAGK, C19-K1, C19-K2 and C19-K3) derivatized with a nonadecanoic alkyl chain. In their acetylated (Ac-) or fluorenylated (Fmoc-) versions, these peptides previously demonstrated the ability to form biocompatible hydrogels potentially suitable as extracellular matrices for tissue engineering or diagnostic MRI applications. In the micromolar range, PAs self-assemble in aqueous solution into nanotapes, or small clusters, resulting in high biocompatibility on HaCat cells up to 72 hours of incubation. Moreover, C19-VAGK also forms a gel at a concentration of 5 wt%.


Assuntos
Nanoestruturas , Nanotubos , Peptídeos/química , Nanoestruturas/química , Estrutura Secundária de Proteína , Cátions
8.
J Pept Sci ; 28(1): e3301, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33491262

RESUMO

Hydrogels (HGs) and nanogels (NGs) have been recently identified as innovative supramolecular materials for many applications in biomedical field such as in tissue engineering, optoelectronic, and local delivery of active pharmaceutical ingredients (APIs). Due to their in vivo biocompatibility, synthetic accessibility, low cost, and tunability, peptides have been used as suitable building blocks for preparation of HGs and NGs formulations. Peptide HGs have shown an outstanding potential to deliver small drugs, protein therapeutics, or diagnostic probes, maintaining the efficacy of their loaded molecules, preventing degradation phenomena, and responding to external physicochemical stimuli. In this review, we discuss the possible use of peptide-based HGs and NGs as vehicles for the delivery of the anticancer drug doxorubicin (Dox). This anthracycline is clinically used for leukemia, stomach, lung, ovarian, breast, and bladder cancer therapy. The loading of Dox into supramolecular systems (liposomes, micelles, hydrogels, and nanogels) allows reducing its cardiotoxicity. According to a primary sequence classification of the constituent peptide, doxorubicin-loaded systems are here classified in short and ultra-short peptide-based HGs, RGD, or RADA-peptide-based HGs and peptide-based NGs.


Assuntos
Antineoplásicos , Hidrogéis , Doxorrubicina , Sistemas de Liberação de Medicamentos , Micelas , Peptídeos
9.
Int J Mol Sci ; 23(7)2022 Apr 03.
Artigo em Inglês | MEDLINE | ID: mdl-35409352

RESUMO

Extracellular vesicles (EVs) shuttle proteins, RNA, DNA, and lipids crucial for cell-to-cell communication. Recent findings have highlighted that EVs, by virtue of their cargo, may also contribute to breast cancer (BC) growth and metastatic dissemination. Indeed, EVs are gaining great interest as non-invasive cancer biomarkers. However, little is known about the biological and physical properties of EVs from malignant BC lesions, and even less is understood about EVs from non-malignant lesions, such as breast fibroadenoma (FAD), which are clinically managed using conservative approaches. Thus, for this pilot study, we attempted to purify and explore the proteomic profiles of EVs from benign breast lesions, HER2+ BCs, triple-negative BCs (TNBCs), and continuous BC cell lines (i.e., BT-549, MCF-10A, and MDA-MB-231), combining experimental and semi-quantitative approaches. Of note, proteome-wide analyses showed 49 common proteins across EVs harvested from FAD, HER2+ BCs, TNBCs, and model BC lines. This is the first feasibility study evaluating the physicochemical composition and proteome of EVs from benign breast cells and primary and immortalized BC cells. Our preliminary results hold promise for possible implications in precision medicine for BC.


Assuntos
Neoplasias da Mama , Vesículas Extracelulares , Fibroadenoma , Neoplasias da Mama/metabolismo , Linhagem Celular Tumoral , Vesículas Extracelulares/metabolismo , Feminino , Fibroadenoma/metabolismo , Fibroadenoma/patologia , Flavina-Adenina Dinucleotídeo/metabolismo , Humanos , Projetos Piloto , Proteoma/metabolismo , Proteômica/métodos
10.
Chemistry ; 27(57): 14307-14316, 2021 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-34314536

RESUMO

Peptides and nucleic acids can self-assemble to give supramolecular structures that find application in different fields, ranging from the delivery of drugs to the obtainment of materials endowed with optical properties. Forces that stabilize the "suprastructures" typically are hydrogen bonds or aromatic interactions; in case of nucleic acids, Watson-Crick pairing drives self-assembly while, in case of peptides, backbone hydrogen bonds and interactions between aromatic side chains trigger the formation of structures, such as nanotubes or ribbons. Molecules containing both aromatic peptides and nucleic acids could in principle exploit different forces to self-assemble. In this work we meant to investigate the self-assembly of mixed systems, with the aim to understand which forces play a major role and determine formation/structure of aggregates. We therefore synthesized conjugates of the peptide FF to the peptide nucleic acid dimer "gc" and characterized their aggregates by different spectroscopic techniques, including NMR, CD and fluorescence.


Assuntos
Ácidos Nucleicos Peptídicos , Ligação de Hidrogênio , Peptídeos , Fenilalanina
11.
Chemistry ; 27(60): 14886-14898, 2021 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-34498321

RESUMO

Peptide-based hydrogels, originated by multiscale self-assembling phenomenon, have been proposed as multivalent tools in different technological areas. Structural studies and molecular dynamics simulations pointed out the capability of completely aromatic peptides to gelificate if hydrophilic and hydrophobic forces are opportunely balanced. Here, the effect produced by the introduction of a Cys residue in the heteroaromatic sequence of (FY)3 and in its PEGylated variant was evaluated. The physicochemical characterization indicates that both FYFCFYF and PEG8-FYFCFYF are able to self-assemble in supramolecular nanostructures whose basic cross-ß motif resembles the one detected in the ancestor (FY)3 assemblies. However, gelification occurs only for FYFCFYF at a concentration of 1.5 wt%. After cross-linking of cysteine residues, the hydrogel undergoes to an improvement of the rigidity compared to the parent (FY)3 assemblies as suggested by the storage modulus (G') that increases from 970 to 3360 Pa. The mechanical properties of FYFCFYF are compatible with its potential application in bone tissue regeneration. Moreover, the avalaibility of a Cys residue in the middle of the peptide sequence could allow the hydrogel derivatization with targeting moieties or with biologically relevant molecules.


Assuntos
Cisteína , Hidrogéis , Sequência de Aminoácidos , Simulação de Dinâmica Molecular , Peptídeos
12.
Chemphyschem ; 22(21): 2215-2221, 2021 11 04.
Artigo em Inglês | MEDLINE | ID: mdl-34496136

RESUMO

Analysis of the intrinsic UV-visible fluorescence exhibited by self-assembling amyloid-like peptides in solution and in solid the state highlights that their physical state has a profound impact on the optical properties. In the solid state, a linear dependence of the fluorescence emission peaks as a function of excitation wavelength is detected. On the contrary, an excitation-independent emission is observed in solution. The present findings constitute a valuable benchmark for current and future explanations of the fluorescence emission by amyloids.


Assuntos
Amiloide/química , Fluorescência , Peptídeos/química , Soluções , Raios Ultravioleta
13.
Int J Mol Sci ; 23(1)2021 Dec 30.
Artigo em Inglês | MEDLINE | ID: mdl-35008834

RESUMO

Autoimmune endocrine disorders, such as type 1 diabetes (T1D) and thyroiditis, at present are treated with only hormone replacement therapy. This emphasizes the need to identify personalized effective immunotherapeutic strategies targeting T and B lymphocytes. Among the genetic variants associated with several autoimmune disorders, the C1858T polymorphism of the protein tyrosine phosphatase non-receptor type 22 (PTPN22) gene, encoding for Lyp variant R620W, affects the innate and adaptive immunity. We previously exploited a novel personalized immunotherapeutic approach based on siRNA delivered by liposomes (lipoplexes) that selectively inhibit variant allele expression. In this manuscript, we improved lipoplexes carrying siRNA for variant C1858T by functionalizing them with Fab of Rituximab antibody (RituxFab-Lipoplex) to specifically target B lymphocytes in autoimmune conditions, such as T1D. RituxFab-Lipoplexes specifically bind to B lymphocytes of the human Raji cell line and of human PBMC of healthy donors. RituxFab-Lipoplexes have impact on the function of B lymphocytes of T1D patients upon CpG stimulation showing a higher inhibitory effect on total cell proliferation and IgM+ plasma cell differentiation than the not functionalized ones. These results might open new pathways of applicability of RituxFab-Lipoplexes, such as personalized immunotherapy, to other autoimmune disorders, where B lymphocytes are the prevalent pathogenic immunocytes.


Assuntos
Linfócitos B/imunologia , Técnicas de Transferência de Genes , Fragmentos Fab das Imunoglobulinas/imunologia , Lipídeos/química , Mutação/genética , Proteína Tirosina Fosfatase não Receptora Tipo 22/genética , RNA Interferente Pequeno/administração & dosagem , Rituximab/imunologia , Sequência de Aminoácidos , Linfócitos B/efeitos dos fármacos , Linhagem Celular , Dicroísmo Circular , Difusão Dinâmica da Luz , Humanos , Lipossomos , Ativação Linfocitária/imunologia , Fenótipo , Proteólise/efeitos dos fármacos , Rituximab/química , Rituximab/farmacologia
14.
Soft Matter ; 16(30): 7006-7017, 2020 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-32638818

RESUMO

Over the last few years, hydrogels have been proposed for many biomedical applications, including drug delivery systems and scaffolds for tissue engineering. In particular, peptides have been envisioned as excellent candidates for the development of hydrogel materials, due to their intrinsic biocompatibility, ease of handling, and intrinsic biodegradability. Recently, we developed a novel hybrid polymer-peptide conjugate, PEG8-(FY)3, which is able to self-assemble into a self-supporting soft hydrogel over dry and wet surfaces as demonstrated by molecular dynamics simulation. Here, we describe the synthesis and supramolecular organization of six novel hexapeptides rationally designed by punctual chemical modification of the primary peptide sequence of the ancestor peptide (FY)3. Non-coded amino acids were incorporated by replacing the phenylalanine residue with naphthylalanine (Nal) and tyrosine with dopamine (Dopa). We also studied the effect of the modification of the side chain and the corresponding PEGylated peptide analogues, on the structural and mechanical properties of the hydrogel. Secondary structure, morphology and rheological properties of all the peptide-based materials were assessed by various biophysical tools. The in vitro biocompatibility of the supramolecular nanostructures was also evaluated on fibroblast cell lines. We conclude that the PEG8-(Nal-Dopa)3 hydrogel possesses the right properties to serve as a scaffold and support cell growth.


Assuntos
Hidrogéis , Nanoestruturas , Peptídeos , Estrutura Secundária de Proteína , Engenharia Tecidual
15.
Molecules ; 25(15)2020 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-32751321

RESUMO

Recently, nanogels have been identified as innovative formulations for enlarging the application of hydrogels (HGs) in the area of drug delivery or in diagnostic imaging. Nanogels are HGs-based aggregates with sizes in the range of nanometers and formulated in order to obtain injectable preparations. Regardless of the advantages offered by peptides in a hydrogel preparation, until now, only a few examples of peptide-based nanogels (PBNs) have been developed. Here, we describe the preparation of stable PBNs based on Fmoc-Phe-Phe-OH using three different methods, namely water/oil emulsion (W/O), top-down, and nanogelling in water. The effect of the hydrophilic-lipophilic balance (HLB) in the formulation was also evaluated in terms of size and stability. The resulting nanogels were found to encapsulate the anticancer drug doxorubicin, chosen as the model drug, with a drug loading comparable with those of the liposomes.


Assuntos
Nanogéis/química , Peptídeos/química , Doxorrubicina/química , Doxorrubicina/farmacologia , Portadores de Fármacos/química , Liberação Controlada de Fármacos , Emulsões , Interações Hidrofóbicas e Hidrofílicas , Estrutura Molecular , Nanomedicina Teranóstica
16.
Chemistry ; 25(65): 14850-14857, 2019 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-31566814

RESUMO

Studies carried out in recent decades have revealed that the ability to self-assemble is a widespread property among biomolecules. Small nucleic acid moieties or very short peptides are able to generate intricate assemblies endowed with remarkable structural and spectroscopic properties. Herein, the structural/spectroscopic characterization of aggregates formed by nucleobases and peptide nucleic acid (PNA)-peptide conjugates are reported. At high concentration, all studied nucleobases form aggregates characterized by previously unreported fluorescence properties. The conjugation of these bases, as PNA derivatives, to the dipeptide Phe-Phe leads to the formation of novel hybrid assemblies, which are characterized by an amyloid-like association of the monomers. Although these compounds share the same basic cross-ß motif, the nature and number of PNA units have an important impact on both the level of structural order and the intrinsic fluorescence of the self-assembled nanostructure.


Assuntos
Ácidos Nucleicos Peptídicos/química , Fenilalanina/análogos & derivados , Dipeptídeos , Microscopia de Fluorescência , Nanoestruturas/química , Fenilalanina/química , Difração de Raios X
17.
Chemphyschem ; 20(21): 2774-2782, 2019 11 05.
Artigo em Inglês | MEDLINE | ID: mdl-31544288

RESUMO

Diphenylalanine (FF) represents one of the most studied self-assembling peptides. As a consequence of non-covalent interactions (aromatic stacking and hydrogen bonds), FF is able to generate different nanoarchitectures, proposed in the last years as innovative tools for several applications. The identification of the relationship between the chemical building block composition and the supramolecular structure of final material is the objective of intense research. Different FF analogues were synthetized and studied. At the state of art, in the high number of FF derivatives, PEGylation has not been studied yet, notwithstanding its role has been demonstrated for longer poly-phenylalanine peptides. Herein, we describe the synthesis and the supramolecular behavior of two PEGylated-FF derivatives, PEG2-FF and PEG6-FF, in which the zwitterionic FF has been derivatized at the N-terminus with two or six ethoxylic moieties, respectively. Spectroscopic methodologies (fluorescence, circular dichroism, Fourier transform infrared) allowed the identification of their secondary structure and the calculation of the critical aggregation concentration. PEGylation of the dipeptide induces a modification of the conformational organization from nanotubes with hexagonal symmetry to ß-sheet rich fibrils. This structural organization confers photoluminescence features to the supramolecular structures.

18.
Soft Matter ; 15(3): 487-496, 2019 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-30601569

RESUMO

Short peptides or single amino acids are interesting building blocks for fabrication of hydrogels, frequently used as extracellular matrix-mimicking scaffolds for cell growth in tissue engineering. The combination of two or more peptide hydrogelators could allow obtaining different materials exhibiting new architectures, tunable mechanical properties, high stability and improved biofunctionality. Here we report on the synthesis, formulation and multi-scale characterization of peptide-based mixed hydrogels formed by the low molecular weight Fmoc-FF (Nα-fluorenylmethyloxycarbonyl diphenylalanine) hydrogelator and of the PEG8-(FY)3 hexapeptide, containing three repetitions of the Phe-Tyr motif and a PEG moiety at its N-terminus. Mixed hydrogels were also prepared by replacing PEG8-(FY)3 with its analogue (FY)3, without the PEG moiety. Rheology analysis confirmed the improved mechanical features of the multicomponent gels prepared at two different ratios (2/1 or 1/1, v/v). However, the presence of the hydrophilic PEG polymeric moiety causes a slowing down of the gel kinetic formation (from 42 to 18 minutes) and a decrease of the gel rigidity (G' from 9 to 6 kPa). Preliminary in vitro biocompatibility and cell adhesion assays performed on Chinese hamster ovarian (CHO) cells suggest a potential employment of these multicomponent hydrogels as exogenous scaffold materials for tissue engineering.


Assuntos
Fluorenos/química , Hidrogéis/síntese química , Oligopeptídeos/química , Fenilalanina/análogos & derivados , Alicerces Teciduais/química , Animais , Células CHO , Cricetinae , Cricetulus , Dipeptídeos , Fenilalanina/química , Reologia , Alicerces Teciduais/efeitos adversos
19.
J Pept Sci ; 25(5): e3157, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30767370

RESUMO

Magnetic resonance imaging (MRI) is one of the most important clinic diagnostic tool used to obtain high-quality body images. The administration of low-molecular-weight Gd complex-based MRI contrast agents (CAs) permits to increase the 1 H relaxation rate of nearby water molecules, thus modulating signal intensity and contrast enhancement. Even if highly accurate, MRI modality suffers from its low sensitivity. Moreover, low-molecular-weight CAs rapidly equilibrate between the intravascular and extravascular spaces after their administration. In order to improve their sensitivity and limit the extravasation phenomenon, several macromolecular and supramolecular multimeric gadolinium complexes (dendrimers, polymers, carbon nanostructures, micelles, and liposomes) have been designed until now. Because of their biocompatibility, low immunogenicity, low cost, and easy synthetic modification, peptides are attractive building blocks for the fabbrication of novel materials for biomedical applications. We report on the state of the art of supramolecular CAs obtained by self-assembly of three different classes of building blocks containing a peptide sequence, a gadolinium complex, and, if necessary, a third functional portion achieving the organization process.


Assuntos
Meios de Contraste/química , Gadolínio/química , Imageamento por Ressonância Magnética , Peptídeos/química , Meios de Contraste/síntese química , Humanos , Substâncias Macromoleculares , Micelas , Peptídeos/síntese química
20.
Molecules ; 24(2)2019 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-30669445

RESUMO

Peptides of natural and synthetic sources are compounds operating in a wide range of biological interactions. They play a key role in biotechnological applications as both therapeutic and diagnostic tools. They are easily synthesized thanks to solid-phase peptide devices where the amino acid sequence can be exactly selected at molecular levels, by tuning the basic units. Recently, peptides achieved resounding success in drug delivery and in nanomedicine smart applications. These applications are the most significant challenge of recent decades: they can selectively deliver drugs to only pathological tissues whilst saving the other districts of the body. This specific feature allows a reduction in the drug side effects and increases the drug efficacy. In this context, peptide-based aggregates present many advantages, including biocompatibility, high drug loading capacities, chemical diversity, specific targeting, and stimuli responsive drug delivery. A dual behavior is observed: on the one hand they can fulfill a structural and bioactive role. In this review, we focus on the design and the characterization of drug delivery systems using peptide-based carriers; moreover, we will also highlight the peptide ability to self-assemble and to actively address nanosystems toward specific targets.


Assuntos
Sistemas de Liberação de Medicamentos/tendências , Nanoestruturas/química , Peptídeos/química , Aminoácidos/química , Transporte Biológico , Dipeptídeos , Liberação Controlada de Fármacos , Humanos , Terapia de Alvo Molecular , Nanomedicina , Fenilalanina/análogos & derivados , Fenilalanina/química , Multimerização Proteica
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