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1.
Metabolomics ; 18(11): 92, 2022 11 13.
Artigo em Inglês | MEDLINE | ID: mdl-36371785

RESUMO

INTRODUCTION: PKCδ is ubiquitously expressed in mammalian cells and its dysregulation plays a key role in the onset of several incurable diseases and metabolic disorders. However, much remains unknown about the metabolic pathways and disturbances induced by PKC deficiency, as well as the metabolic mechanisms involved. OBJECTIVES: This study aims to use metabolomics to further characterize the function of PKC from a metabolomics standpoint, by comparing the full serum metabolic profiles of PKC deficient mice to those of wild-type mice. METHODS: The serum metabolomes of PKCδ knock-out mice were compared to that of a wild-type strain using a GCxGC-TOFMS metabolomics research approach and various univariate and multivariate statistical analyses. RESULTS: Thirty-seven serum metabolite markers best describing the difference between PKCδ knock-out and wild-type mice were identified based on a PCA power value > 0.9, a t-test p-value < 0.05, or an effect size > 1. XERp prediction was also done to accurately select the metabolite markers within the 2 sample groups. Of the metabolite markers identified, 78.4% (29/37) were elevated and 48.65% of these markers were fatty acids (18/37). It is clear that a total loss of PKCδ functionality results in an inhibition of glycolysis, the TCA cycle, and steroid synthesis, accompanied by upregulation of the pentose phosphate pathway, fatty acids oxidation, cholesterol transport/storage, single carbon and sulphur-containing amino acid synthesis, branched-chain amino acids (BCAA), ketogenesis, and an increased cell signalling via N-acetylglucosamine. CONCLUSION: The charaterization of the dysregulated serum metabolites in this study, may represent an additional tool for the early detection and screening of PKCδ-deficiencies or abnormalities.


Assuntos
Metabolômica , Proteína Quinase C-delta , Camundongos , Animais , Metabolômica/métodos , Proteína Quinase C-delta/genética , Camundongos Knockout , Metaboloma , Biomarcadores , Ácidos Graxos , Mamíferos
2.
Appl Microbiol Biotechnol ; 103(9): 3669-3682, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30911788

RESUMO

Some members of the Bacillus velezensis (Bv) group (e.g., Bv FZB42T and AS3.43) were previously assigned grouping with B. subtilis and B. amyloliquefaciens, based on the fact that they shared a 99% DNA-DNA percentage phylogenetic similarity. However, hinging on current assessments of the pan-genomic reassignments, the differing phylogenomic characteristics of Bv from B. subtilis and B. amyloliquefaciens are now better understood. Within this re-grouping/reassignment, the various strains within the Bv share a close phylogenomic resemblance, and a number of these strains have received a lot of attention in recent years, due to their genomic robustness, and the growing evidence for their possible utilization in the agricultural industry for managing plant diseases. Only a few applications for their use medicinally/pharmaceutically, environmentally, and in the food industry have been reported, and this may be due to the fact that the majority of those strains investigated are those typically occurring in soil. Although the intracellular unique biomolecules of Bv strains have been revealed via in silico genome modeling and investigated using transcriptomics and proteomics, a further inquisition into the Bv metabolome using newer technologies such as metabolomics could elucidate additional applications of this economically relevant Bacillus species, beyond that of primarily the agricultural sector.


Assuntos
Bacillus/classificação , Bacillus/metabolismo , Filogenia , Bacillus/genética , Bacillus/isolamento & purificação , Microbiologia de Alimentos , Genoma Bacteriano , Genômica , Microbiologia Industrial , Metaboloma
3.
Arch Microbiol ; 200(8): 1239-1255, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-29934785

RESUMO

This review presents biocontrol agents employed to alleviate the deleterious effect of the pathogen Fusarium graminearum on maize. The control of this mycotoxigenic phytopathogen remains elusive despite the elaborate research conducted on its detection, identification, and molecular fingerprinting. This could be attributed to the fact that in vitro and greenhouse biocontrol studies on F. graminearum have exceeded the number of field studies done. Furthermore, along with the variances seen among these F. graminearum suppressing biocontrol strains, it is also clear that the majority of research done to tackle F. graminearum outbreaks was on wheat and barley cultivars. Most fusariosis management related to maize targeted other members of Fusarium such as Fusarium verticillioides, with biocontrol strains from the genera Bacillus and Pseudomonas being used frequently in the experiments. We highlight relevant current techniques needed to identify an effective biofungicide for maize fusariosis and recommend alternative approaches to reduce the scarcity of data for indigenous maize field trials.


Assuntos
Agentes de Controle Biológico , Fusarium , Doenças das Plantas/prevenção & controle , Zea mays/microbiologia , Bacillus/fisiologia , Pseudomonas/fisiologia
4.
Microbiol Resour Announc ; 13(4): e0120123, 2024 Apr 11.
Artigo em Inglês | MEDLINE | ID: mdl-38456700

RESUMO

Genomic features of Staphylococcus auricularis PAPLE_T1 isolated from waste sample of Carica papaya obtained from Lagos State, Nigeria, revealed its putative capability to synthesize valuable secondary metabolites. S. auricularis PAPLE_T1 has a 2.4 Mb genome and could be useful as biological agro-antibiotics, for soil bioremediation and in biotechnological industry.

5.
Microbiol Resour Announc ; 12(12): e0075323, 2023 Dec 14.
Artigo em Inglês | MEDLINE | ID: mdl-37909746

RESUMO

Lysinibacillus fusiformis PwPw_T2 isolated from deteriorating Ananas comosus sample collected from Lagos State, Nigeria putatively possesses genomic features like potential enzymes catalyzing acetic acid production and xenobiotic compounds degradation via various pathways as indicated by its genome sequences. These could make the organism relevant in food waste valorization and micro-biotechnology.

6.
Biotechnol Rep (Amst) ; 25: e00425, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32099821

RESUMO

Phytopathogens from the Alternaria sp., Fusarium sp., Penicillium sp., and Pseudomonas sp. and their toxigenic metabolites - alternariol, fumonisin, citrinin, and coronatine respectively, negatively impact crop yields and sales by eliciting plant diseases and/or causing human and veterinary toxicoses upon the consumption of contaminated food. These phytopathogens and their associated toxins, however, are present and most likely in undetectable concentrations pre-harvest and post-harvest of many major staple crops. Metabolomic approaches have been used extensively for better characterizing and diagnosing human disease, plant disease and, their etiological agents. Their use in agro-industrial research focusing specifically on tripartite (plant - toxicogenic microbe - beneficial microbe) interactions is, however, limited. Since new approaches for eradicating food-borne pathogens, increasing crop productivity and improving agro-international trade are being sought worldwide, the consequent integration of metabolomic approaches and perspectives in crop protection strategies for better understanding plant - toxicogenic microbe - beneficial microbe interaction in tandem is discussed.

7.
Microbiologyopen ; 8(6): e00742, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-30358165

RESUMO

The results of this study indicate that the maize rhizosphere remains a reservoir for microbial strains with unique beneficial properties. The study sought to provide an indigenous Bacillus strain with a bioprotective potential to alleviate maize fusariosis in South Africa. We selected seven Bacillus isolates (MORWBS1.1, MARBS2.7, VERBS5.5, MOREBS6.3, MOLBS8.5, MOLBS8.6, and NWUMFkBS10.5) with biosuppressive effects against two maize fungal pathogens (Fusarium graminearum and Fusarium culmorum) based on 16S rDNA gene characterization and lipopeptide gene analysis. The PCR analysis revealed that lipopeptide genes encoding the synthesis of iturin, surfactin, and fengycin might be responsible for their antifungal activities. Few of the isolates also showed possible biosurfactant capability, and their susceptibility to known antibiotics is indicative of their eco-friendly attributes. In addition, in silico genomic analysis of our best isolate (Bacillus velezensis NWUMFkBS10.5) and characterization of its active metabolite with FTIR, NMR, and ESI-Micro-Tof MS confirmed the presence of valuable genes clusters and metabolic pathways. The versatile genomic potential of our Bacillus isolate emphasizes the continued relevance of Bacillus spp. in biological management of plant diseases.


Assuntos
Bacillus/química , Fusarium/efeitos dos fármacos , Lipopeptídeos/farmacologia , Bacillus/genética , Bacillus/isolamento & purificação , Bacillus/metabolismo , Fungicidas Industriais/química , Fungicidas Industriais/metabolismo , Fungicidas Industriais/farmacologia , Fusarium/crescimento & desenvolvimento , Genoma Bacteriano , Lipopeptídeos/química , Lipopeptídeos/genética , Lipopeptídeos/metabolismo , Metabolômica , Filogenia , Doenças das Plantas/microbiologia , Zea mays/microbiologia
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