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1.
Cell Mol Biol (Noisy-le-grand) ; 70(4): 8-14, 2024 Apr 28.
Artigo em Inglês | MEDLINE | ID: mdl-38678633

RESUMO

The existence of diverse microbes in unprocessed camel milk poses a significant threat to the well-being of a large population, especially infants and toddlers. The objective of this study was to ascertain the existence of microorganisms in unprocessed raw camel milk by employing a molecular-based technique in combination with a histological examination of bacteria. The identification of microbial species was achieved by employing PCR amplification and sequencing of 16s rRNA gene fragments. Various micorganisms found includes the probiotic Lactobacillus species, Staphylococcus succinic, Macrococcus casealyticus, Bacillus cohnii, and Salinicoccus kunmingensis. To prevent microbial contamination in raw milk, it is necessary to adequately heat or pasteurise the milk and to wash and sterilise the udder before milking the camel. This is because raw milk contains microbes that cause multiple diseases. Moreover, in the current era of the COVID-19 pandemics, ensuring proper sanitary conditions in milk and its derivatives might potentially mitigate the transmission of various diseases among consumers shortly. Keywords: camel, microbiota, 16s rRNA gene, PCR.


Assuntos
Camelus , Microbiota , Leite , Reação em Cadeia da Polimerase , RNA Ribossômico 16S , Camelus/microbiologia , Animais , Leite/microbiologia , RNA Ribossômico 16S/genética , Microbiota/genética , Reação em Cadeia da Polimerase/métodos , Bactérias/genética , Bactérias/isolamento & purificação , Bactérias/classificação
2.
Environ Sci Pollut Res Int ; 28(44): 62266-62273, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34184228

RESUMO

We conducted the current analysis to determine the potential role of measles vaccination in the context of the spread of COVID-19. Data were extracted from the World Health Organization's (WHO) Global Health Observatory data repository about the measles immunization coverage estimates and correlated to overall morbidity and mortality for COVID-19 among different countries. Data were statistically analyzed to calculate the Spearman rank correlation coefficient (rho). There was a significant positive correlation between the vaccine coverage (%) and new cases per one million populations (rho = 0.24; p-value = 0.025); however, this correlation was absent in deaths per one million populations (rho = 0.17; p-value = 0.124). On further analysis of the effect of first reported year of vaccination policy, there was no significant correlation with both of total cases per one million populations (rho = 0.11; p-value = 0.327) and deaths per one million populations (rho = -0.02; p-value = 0.829). Claims regarding the possible protective effect of measles vaccination seem to be doubtful.


Assuntos
COVID-19 , Sarampo , Estudos Epidemiológicos , Humanos , Lactente , Sarampo/epidemiologia , Sarampo/prevenção & controle , Morbidade , SARS-CoV-2 , Vacinação , Cobertura Vacinal
3.
Environ Sci Pollut Res Int ; 28(26): 34611-34618, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33651292

RESUMO

We conducted the current analysis to determine the potential role of polio vaccination in the context of the spread of COVID-19. Data were extracted from the World Health Organization's (WHO) Global Health Observatory data repository regarding the polio immunization coverage estimates and correlated to the overall morbidity and mortality for COVID-19 among different countries. Data were analyzed using R software version 4.0.2. Mean and standard deviation were used to represent continuous variables while we used frequencies and percentages to represent categorical variables. The Kruskal-Wallis H test was used for continuous variables since they were not normally distributed. Moreover, the Spearman rank correlation coefficient (rho) was used to determine the relationship between different variables. There was a significantly positive correlation between the vaccine coverage (%) and both of total cases per one million populations (rho = 0.37; p-value < 0.001) and deaths per one million populations (rho = 0.30; p-value < 0.001). Moreover, there was a significant correlation between different income groups and each of vaccine coverage (%) (rho = 0.71; p-value < 0.001), total cases per one million populations (rho = 0.50; p-value < 0.001), and deaths per one million populations (rho = 0.39; p-value < 0.001). All claims regarding the possible protective effect of Polio vaccination do not have any support when analyzing the related data. Polio vaccination efforts should be limited to eradicate the disease from endemic countries; however, there is no evidence to support the immunization with live-attenuated vaccines for the protection against COVID-19.


Assuntos
COVID-19 , Poliomielite , Humanos , Morbidade , Poliomielite/epidemiologia , Poliomielite/prevenção & controle , SARS-CoV-2 , Vacinação , Cobertura Vacinal
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