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1.
Heliyon ; 10(7): e28967, 2024 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-38601589

RESUMO

Plant diseases annually cause damage and loss of much of the crop, if not its complete destruction, and this constitutes a significant challenge for farm owners, governments, and consumers alike. Therefore, identifying and classifying diseases at an early stage is very important in order to sustain local and global food security. In this research, we designed a new method to identify plant diseases by combining transfer learning and Gravitational Search Algorithm (GSA). Two state-of-the-art pretrained models have been adopted for extracting features in this study, which are MobileNetV2 and ResNe50V2. Multilayer feature extraction is applied in this study to ensure representations of plant leaves from different levels of abstraction for precise classification. These features are then concatenated and passed to GSA for optimizing them. Finally, optimized features are passed to Multinomial Logistic Regression (MLR) for final classification. This integration is essential for categorizing 18 different types of infected and healthy leaf samples. The performance of our approach is strengthened by a comparative analysis that incorporates features optimized by the Genetic Algorithm (GA). Additionally, the MLR algorithm is contrasted with K-Nearest Neighbors (KNN). The empirical findings indicate that our model, which has been refined using GSA, achieves very high levels of precision. Specifically, the average precision for MLR is 99.2%, while for KNN it is 98.6%. The resulting results significantly exceed those achieved with GA-optimized features, thereby highlighting the superiority of our suggested strategy. One important result of our study is that we were able to decrease the number of features by more than 50%. This reduction greatly reduces the processing requirements without sacrificing the quality of the diagnosis. This work presents a robust and efficient approach to the early detection of plant diseases. The work demonstrates the utilization of sophisticated computational methods in agriculture, enabling the development of novel data-driven strategies for plant health management, therefore enhancing worldwide food security.

2.
Zhonghua Zhong Liu Za Zhi ; 25(5): 433-6, 2003 Sep.
Artigo em Zh | MEDLINE | ID: mdl-14575563

RESUMO

OBJECTIVE: To investigate whether hepatitis B x protein (HBx) stimulates vascular endothelial growth factor (VEGF) through hypoxia inducible factor-1 (HIF-1 alpha) pathway. METHODS: Two plasmids including pIRES-EGFP-HBx and pTK-Hyg were co-transfected to a hepatocellular carcinoma cell line SMMC-7721. With fluorescence-positive and fluorescence-negative hygromycin-resistant colonies selected, expressions of VEGF and HIF-1 alpha in protein or/and mRNA level were detected. RESULTS: Fluorescence-positive cells were stably integrated with HBx, in which expression of HIF-1 alpha and VEGF were upregulated. Fluorescence-negative cells did not express HBx, VEGF or HIF-1 alpha. CONCLUSION: HBx can activate VEGF through HIF-1 alpha pathway.


Assuntos
Transativadores/fisiologia , Fatores de Transcrição/fisiologia , Fator A de Crescimento do Endotélio Vascular/genética , Linhagem Celular Tumoral , Regulação da Expressão Gênica , Humanos , Subunidade alfa do Fator 1 Induzível por Hipóxia , Fatores de Transcrição/genética , Fator A de Crescimento do Endotélio Vascular/fisiologia , Proteínas Virais Reguladoras e Acessórias
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