Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Mais filtros

Base de dados
Ano de publicação
Tipo de documento
Intervalo de ano de publicação
1.
J Oral Maxillofac Surg ; 72(5): 1000.e1-11, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24742484

RESUMO

PURPOSE: "Allograft revitalization" is a process in which cadaveric bone is used to generate well-vascularized living bone. We had previously found that porcine allograft hemimandibles filled with autologous adipose-derived stem cells (ASCs) and recombinant human bone morphogenetic protein-2-soaked absorbable collagen sponge (rhBMP-2/ACS) were completely replaced by vascularized bone, provided the construct had been incubated within a periosteal envelope. The present study sought to deepen our understanding of allograft revitalization by investigating the individual contributions of ASCs and rhBMP-2 in the process and the mechanical properties of the revitalized allograft. MATERIALS AND METHODS: Porcine allograft hemimandible constructs were implanted bilaterally into rib periosteal envelopes in 8 pigs. To examine the contributions of ASCs and rhBMP-2, the following groups were assessed: group 1, periosteum alone; group 2, periosteum+ASCs; group 3, periosteum+rhBMP-2/ACS; and group 4, periosteum+ASCs+rhBMP-2/ACS. After 8 weeks, the allograft constructs were harvested for micro-computed tomography (CT) and histologic analyses and 3-point bending to assess the strength. RESULTS: On harvesting, the constructs receiving rhBMP-2/ACS had significantly greater bone shown by micro-CT than those receiving periosteum only (51,463 vs. 34,310 mm3; P = .031). The constructs receiving ASCs had increased bone compared to group 1 (periosteum only), although not significantly (P = .087). The combination of rhBMP-2/ACS with ASCs produced bone (50,399 mm3) equivalent to that of the constructs containing rhBMP-2/ACS only. The 3-point bending tests showed no differences between the 4 groups and a nonimplanted allograft or native mandible (P = .586), suggesting the absence of decreased strength of the allograft bone when revitalized. CONCLUSIONS: These data have shown that rhBMP-2/ACS significantly stimulates new bone formation by way of allograft revitalization and that the revitalized allograft has equivalent mechanical strength to native bone.


Assuntos
Aloenxertos/fisiologia , Regeneração Óssea/fisiologia , Engenharia Tecidual/métodos , Implantes Absorvíveis , Tecido Adiposo/citologia , Animais , Autoenxertos/transplante , Fenômenos Biomecânicos , Proteína Morfogenética Óssea 2/uso terapêutico , Técnicas de Cultura de Células , Diferenciação Celular/fisiologia , Condrogênese/fisiologia , Colágeno , Feminino , Humanos , Mandíbula/patologia , Transplante de Células-Tronco Mesenquimais/métodos , Modelos Animais , Osteogênese/fisiologia , Periósteo/cirurgia , Maleabilidade , Proteínas Recombinantes/uso terapêutico , Estresse Mecânico , Suínos , Preservação de Tecido/métodos , Alicerces Teciduais/química , Coleta de Tecidos e Órgãos , Fator de Crescimento Transformador beta/uso terapêutico , Microtomografia por Raio-X
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA