On the trail of a critically endangered fungus: A world-first application of wildlife detection dogs to fungal conservation.

Plant and animal conservation have benefited from the assistance of wildlife detection dogs (WDDs) since 1890, but their application to fungal conservation has not been trialed. In a world-first, we tested the effectiveness of WDDs and human surveyors when searching for experimentally outplanted fungi in natural habitat. We focused on a critically endangered fungus from Australia, Hypocreopsis amplectens, and showed that a WDD outperformed a human surveyor: our WDD detected a greater proportion of targets, had a faster time to first discovery, and had fewer false negatives. Our study highlights the tremendous potential for WDDs to enhance fungal conservation by demonstrating their utility in one of the most challenging fungal systems: a rare species with low population densities and low volatility. Our findings suggest that the application of WDDs to fungal conservation should enhance continuing efforts to document and conserve an understudied kingdom that is threatened by habitat loss and climate change.

Octopus djinda (Cephalopoda: Octopodidae): a new member of the Octopus vulgaris group from southwest Australia.

A new Octopus Cuvier, 1797 species, Octopus djinda Amor, 2021 (previously treated as O. cf. tetricus and O. aff. tetricus), is described from the shallow waters off southwest Australia. This species was classified as conspecific with O. tetricus Gould, 1852 from Australias east coast and New Zealand but is shown here to be morphologically and genetically distinct. This description is based on 25 individuals across three localities in southwest Australia, encompassing most of its distribution. Greater and non-overlapping sucker counts on the males hectocotylised arm delimit east and west coast forms. DNA barcoding using cytochrome c oxidase subunit I also successfully differentiates between these taxa; 13 polymorphisms along a 349 bp partial fragment (3.7% sequence divergence). A close relative of the O. vulgaris Cuvier, 1797 species-group, O. djinda, sp. nov. supports a highly productive fishery and is currently one of two octopod fisheries worldwide to have received sustainable certification from the Marine Stewardship Council. The taxonomic description presented here provides formal recognition of the taxonomic status of southwest Australias common octopus, O. djinda, sp. nov. and facilitates appropriate fisheries catch reporting and management.

Characterization of the complete plastid genome of Astelia australiana (J. H. Willis) L. B. Moore (Asteliaceae, Asparagales).

Astelia australiana is a robust understorey plant with a highly restricted distribution in southeastern Australia. Here we report its complete plastid genome. The genome was 157,943 bp in length and comprises a pair of inverted repeats (IRs) of 27,028 bp separated by a large single-copy region (LSC) of 85,699 bp, and a small single-copy region (SSC) of 18,188 bp. The GC content was 37.7%. In total, 132 genes were annotated including 81 protein-coding genes (PCGs), 38 tRNA genes, and 8 rRNA genes. Phylogenetic analysis of the PCGs from A. australiana aligned with those from 10 Asparagales representatives confirms that, based on these taxa, A. australiana is sister to A. pumila and sits within the Asteliaceae.

Allopatric speciation within a cryptic species complex of Australasian octopuses.

Despite extensive revisions over recent decades, the taxonomy of benthic octopuses (Family Octopodidae) remains in a considerable flux. Among groups of unresolved status is a species complex of morphologically similar shallow-water octopods from subtropical Australasia, including: Allopatric populations of Octopus tetricus on the eastern and western coasts of Australia, of which the Western Australian form is speculated to be a distinct or sub-species; and Octopus gibbsi from New Zealand, a proposed synonym of Australian forms. This study employed a combination of molecular and morphological techniques to resolve the taxonomic status of the 'tetricus complex'. Phylogenetic analyses (based on five mitochondrial genes: 12S rRNA, 16S rRNA, COI, COIII and Cytb) and Generalised Mixed Yule Coalescent (GMYC) analysis (based on COI, COIII and Cytb) distinguished eastern and Western Australian O. tetricus as distinct species, while O. gibbsi was found to be synonymous with the east Australian form (BS = >97, PP = 1; GMYC p = 0.01). Discrete morphological differences in mature male octopuses (based on sixteen morphological traits) provided further evidence of cryptic speciation between east (including New Zealand) and west coast populations; although females proved less useful in morphological distinction among members of the tetricus complex. In addition, phylogenetic analyses suggested populations of octopuses currently treated under the name Octopus vulgaris are paraphyletic; providing evidence of cryptic speciation among global populations of O. vulgaris, the most commercially valuable octopus species worldwide.