RESUMO
Here, we introduce a reusable bifunctional polyester/aluminum (PET/Al) air filter for the high efficiency simultaneous capture and inactivation of airborne microorganisms. Both bacteria of Escherichia coli and Staphylococcus epidermidis were collected on the PET/Al filter with a high efficiency rate (â¼99.99%) via the electrostatic interactions between the charged bacteria and fibers without sacrificing pressure drop. The PET/Al filter experienced a pressure drop approximately 10 times lower per thickness compared with a commercial high-efficiency particulate air filter. As the Al nanograins grew on the fibers, the antimicrobial activity against airborne E. coli and S. epidermidis improved to â¼94.8% and â¼96.9%, respectively, due to the reinforced hydrophobicity and surface roughness of the filter. Moreover, the capture and antimicrobial performances were stably maintained during a cyclic washing test of the PET/Al filter, indicative of its reusability. The PET/Al filter shows great potential for use in energy-efficient bioaerosol control systems suitable for indoor environments.
Assuntos
Filtros de Ar , Alumínio , Antibacterianos , Poliésteres , Microbiologia do Ar , Escherichia coli/crescimento & desenvolvimento , Pressão , Staphylococcus epidermidis/crescimento & desenvolvimento , Eletricidade EstáticaRESUMO
Through the direct decomposition of an Al precursor ink AlH3{O(C4H9)2}, we fabricated an Al-coated conductive fiber filter for the efficient electrostatic removal of airborne particles (>99%) with a low pressure drop (~several Pascals). The effects of the electrical and structural properties of the filters were investigated in terms of collection efficiency, pressure drop, and particle deposition behavior. The collection efficiency did not show a significant correlation with the extent of electrical conductivity, as the filter is electrostatically charged by the metallic Al layers forming electrical networks throughout the fibers. Most of the charged particles were collected via surface filtration by Coulombic interactions; consequently, the filter thickness had little effect on the collection efficiency. Based on simulations of various fiber structures, we found that surface filtration can transition to depth filtration depending on the extent of interfiber distance. Therefore, the effects of structural characteristics on collection efficiency varied depending on the degree of the fiber packing density. This study will offer valuable information pertaining to the development of a conductive metal/polymer composite air filter for an energy-efficient and high-performance electrostatic filtration system.
RESUMO
Since the publication of the first report on fish nodaviruses in Korea in 1998, fish nodaviruses have caused widespread epizootic events among various fish species in Korea. However, the genotypes of fish nodaviruses in Korea have not yet been determined due to a lack of information about their nucleotide sequences. In this study, we isolated 5 fish nodaviruses from 4 fish species cultured in 4 different regions in Korea: rock bream Oplegnathus fasciatus, Japanese flounder Paralichthys olivaceus, sevenband grouper Epinephelus septemfasciatus, and grey mullet Mugil cophalus. The full open-reading frame (ORF) encoding the coat protein (1017 nt) was sequenced from each of the 5 fish nodaviruses and the nucleotide sequences were phylogenetically analyzed. Results showed that even though their sequences were not identical, all 5 Korean isolates were clustered in the RGNNV genotype. This is the first report on the phylogenetic analysis of fish nodaviruses from cultured fish in Korea.
Assuntos
Proteínas do Capsídeo/genética , Doenças dos Peixes/virologia , Nodaviridae/classificação , Filogenia , Infecções por Vírus de RNA/veterinária , Sequência de Aminoácidos , Animais , Sequência de Bases , Encéfalo/patologia , Primers do DNA/química , Pesqueiros , Linguados/virologia , Coreia (Geográfico) , Dados de Sequência Molecular , Nodaviridae/isolamento & purificação , Perciformes/virologia , Reação em Cadeia da Polimerase/veterinária , Infecções por Vírus de RNA/virologia , Alinhamento de Sequência , Smegmamorpha/virologiaRESUMO
Here, we demonstrate a new strategy of air filtration based on an Al-coated conductive fibrous filter for high efficient nanoparticulate removals. The conductive fibrous filter was fabricated by a direct decomposition of Al precursor ink, AlH3{O(C4H9)2}, onto surfaces of a polyester air filter via a cost-effective and scalable solution-dipping process. The prepared conductive filters showed a low sheet resistance (<1.0 Ω sq-1), robust mechanical durability and high oxidative stability. By electrostatic force between the charged fibers and particles, the ultrafine particles of 30-400 nm in size were captured with a removal efficiency of â¼99.99%. Moreover, the conductive filters exhibited excellent performances in terms of the pressure drop (â¼4.9 Pa at 10 cm s-1), quality factor (â¼2.2 Pa-1 at 10 cm s-1), and dust holding capacity (12.5 µg mm-2). After being cleaned by water, the filtration efficiency and pressure drop of the conductive filter was perfectly recovered, which indicates its good recyclability. It is expected that these promising features make the conductive fibrous filter have a great potential for use in low-cost and energy-efficient air cleaning devices as well as other relevant research areas.
RESUMO
In 2003, 13 isolates of iridovirus were obtained from cultured flounders Paralichthys olivaceus during epizootics in Korea. The full open reading frames (ORFs) encoding the major capsid protein (MCP) (1362 bp) from the 13 flounder iridoviruses (FLIVs) were sequenced and the deduced amino acid sequences were phylogenetically analyzed. Phylogenetic analysis of the MCP revealed that all 13 FLIVs were the same species as rock bream iridovirus (RBIV), red sea bream iridovirus (RSIV), and infectious spleen and kidney necrosis virus (ISKNV), and were grouped into an unknown genus which was different from the 2 genera known to infect fish, Ranavirus and Lymphocystivirus. This is the first report on the isolation and phylogenetic analysis of the iridovirus of unknown genus from flounders during epizootics.
Assuntos
Proteínas do Capsídeo/genética , Infecções por Vírus de DNA/veterinária , Doenças dos Peixes/virologia , Linguado , Iridoviridae/genética , Filogenia , Sequência de Aminoácidos , Animais , Sequência de Bases , Análise por Conglomerados , Primers do DNA , Iridoviridae/classificação , Coreia (Geográfico) , Microscopia Eletrônica de Transmissão , Dados de Sequência Molecular , Análise de Sequência de DNA , Homologia de Sequência , Especificidade da Espécie , Baço/ultraestrutura , Baço/virologiaRESUMO
BACKGROUND: Plasminogen activator inhibitor-2 (PAI-2) is an enzyme inhibitor which is involved in various biological processes including cell differentiation, tissue regrowth and regeneration. Although PAI-2 has been originally isolated as an extracellular inhibitor of urokinase plasminogen activator (uPA), recent studies indicate that PAI-2 has other intracellular effects in keratinocyte, such as the component of cornified envelope. OBJECTIVE: The aim of this study is to investigate the expression and functional role of PAI-2 during the keratinocyte differentiation. METHODS: We transduced keratinocytes with adenovirus harboring the expression cassette for PAI-2, then examined the effect on keratinocytes differentiation. RESULTS: When cultured epidermal keratinocytes were treated with 1.2 mM calcium, PAI-2 expression was increased time-dependently at both mRNA and protein levels. The calcium-induced PAI-2 expression was abolished by treatment with p38 MAPK inhibitor, while overexpression of MKK6 led to the increase of PAI-2 expression. When PAI-2 was overexpressed by adenoviral transduction, the expression of keratinocyte differentiation markers such as involucrin, keratin 10 and loricrin was markedly increased. Concomitantly, overexpression of PAI-2 resulted in the retardation of cell growth, with the increase of Rb and p53. CONCLUSION: These results suggest that PAI-2 has a role for promoting the differentiation of epidermal keratinocytes.
Assuntos
Diferenciação Celular , Queratinócitos/fisiologia , Inibidor 2 de Ativador de Plasminogênio/metabolismo , Cálcio/metabolismo , Células Epidérmicas , Epiderme/metabolismo , Epiderme/fisiologia , Humanos , Queratina-10/análise , Queratinócitos/citologia , MAP Quinase Quinase 6/metabolismo , Proteínas de Membrana/análise , Precursores de Proteínas/análise , Proteína do Retinoblastoma/análise , Proteína Supressora de Tumor p53/análise , Proteínas Quinases p38 Ativadas por Mitógeno/antagonistas & inibidoresRESUMO
Iridovirus is a causative agent of epizootics among cultured rock bream (Oplegnathus fasciatus) in Korea. Here, we report the complete genomic sequence of rock bream iridovirus (RBIV). The genome of RBIV was 112080 bp long and contained at least 118 putative open reading frames (ORFs), and its genome organization was similar to that of infectious spleen and kidney necrosis virus (ISKNV). Of the RBIV's 118 ORFs, 85 ORFs showed 60-99% amino acid identity to those of ISKNV. Phylogenetic analysis of major capsid protein (MCP), DNA repair protein RAD2, and DNA polymerase type-B family indicated that RBIV is closely related to red sea bream iridovirus (RSIV), Grouper sleepy disease iridovirus (GSDIV), Dwarf gourami iridovirus (DGIV), and ISKNV. The genome sequence provides useful information concerning the evolution and divergence of iridoviruses in cultured fish.