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1.
Am J Physiol Endocrinol Metab ; 282(1): E59-66, 2002 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-11739084

RESUMO

We investigated angiotensinogen (AGT) expression in adipose tissue and liver of Zucker rats during the onset of obesity. The developmental pattern of AGT expression (protein and mRNA) in liver was similar in both genotypes. In inguinal adipose tissue, AGT cell content was similar in suckling and weaned pups in lean rats, whereas it continuously increased with age in obese rats. AGT amount in adipocytes was unaffected by the genotype until weaning. Thereafter, adipocytes from obese rats displayed a significant increase in AGT content that was strengthened with age. Compared with the cell content, the amount of secreted AGT over 24 h was higher, and a genotype effect was observed as early as 14 days of age. Using fat cell populations differing by size, we showed that this AGT oversecretion was not solely related to adipocyte hypertrophy. Our results demonstrate that the fa genotype exerts a control on the production of AGT in a tissue-specific manner, suggesting a local role of AGT in the overdevelopment of adipose tissue.


Assuntos
Tecido Adiposo/metabolismo , Angiotensinogênio/metabolismo , Obesidade/metabolismo , Ratos Zucker/metabolismo , Adipócitos/metabolismo , Tecido Adiposo/citologia , Angiotensinogênio/genética , Animais , Tamanho Celular , Células Cultivadas , Genótipo , Fígado/metabolismo , Obesidade/genética , RNA Mensageiro/metabolismo , Ratos , Valores de Referência
2.
J Nutr ; 133(7): 2239-43, 2003 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-12840186

RESUMO

Fish oil feeding has been shown to limit visceral fat accumulation in insulin-resistant rats. Our goal was to determine whether this finding is due to increased fat mobilization or decreased lipid storage. Adipocytes were isolated from rats fed for 3 wk a diet containing 57.5 g/100 g sucrose and 14 g/100 g lipids as either fish oil (SF) or a mixture of standard oils (SC); there was also a reference group (R). Substituting fish oil for standard oils protected rats from visceral fat hypertrophy, hypertriglyceridemia and hyperglycemia. The stimulation of lipolysis was greater in adipocytes isolated from SF-fed rats than in those from SC-fed rats. Fatty acid synthase (FAS) activity was markedly lower in the liver but not in the adipose tissues of rats fed SF. Lipoprotein lipase (LPL) activity was 2.2-fold higher in the adipose tissues but not in the muscle in rats fed the SF diet than in those fed the SC diet. The decrease in visceral fat in rats fed fish oil could be attributed to decreased plasma triacylglycerol concentration and/or increased lipid mobilization rather than to reduced lipid storage.


Assuntos
Tecido Adiposo/efeitos dos fármacos , Gorduras Insaturadas na Dieta/farmacologia , Óleos de Peixe/farmacologia , Metabolismo dos Lipídeos , Tecido Adiposo/enzimologia , Tecido Adiposo/metabolismo , Animais , Peso Corporal/efeitos dos fármacos , Ácido Graxo Sintases/metabolismo , Isoproterenol/farmacologia , Masculino , Tamanho do Órgão/efeitos dos fármacos , Ratos , Ratos Sprague-Dawley
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