RESUMO
Pythium insidiosum is a fungus-like organism present in subtropical and tropical areas, such as Brazil, known to infect humans and various animal species. P. insidiosum is the etiological agent of pythiosis, an emerging and granulomatous disease characterized mainly by cutaneous and subcutaneous lesions in horses, the principal species affected. Accurate diagnosis of pythiosis and identification of its causal agent by microbiological and serological tests can be often difficult and inconclusive principally for horses and humans. The aim of this study was to evaluate the application of the previously described P. insidiosum-specific nested polymerase chain reaction (PCR) assay to directly detect P. insidiosum DNA in clinical and experimental lesions. Universal fungal primers (ITS1 and ITS4) were used during the first-round of PCR to amplify ITS1, 5.8s, and ITS2. A second-round of PCR was conducted with P. insidiosum-specific primers (PI1 and PI2) to amplify a variable region within this ITS1. In this study, a total of 21 equine clinical samples (kunkers) and 28 specimens from experimentally infected rabbits were analyzed by nested PCR. The first-round of PCR generated 800-base pair products, and the second-round produced 105-base pair amplicons for each P. insidiosum-specific sample; no amplicons were generated in negative control samples. Our results suggest that nested PCR is an important and efficient tool for diagnosis of both endemic (horse samples) and experimental (rabbit samples) pythiosis.
Assuntos
Doenças dos Cavalos/microbiologia , Reação em Cadeia da Polimerase/métodos , Pitiose/veterinária , Pythium/isolamento & purificação , Animais , Brasil , Doenças dos Cavalos/diagnóstico , Cavalos , Pitiose/microbiologia , Pythium/genética , CoelhosRESUMO
In this text we evaluated the in vitro antifungal activities of terbinafine combined with caspofungin, miconazole, ketoconazole, and fluconazole against 17 Pythium insidiosum strains by using the microdilution checkerboard method. Synergistic interactions were observed with terbinafine combined with caspofungin (41.2% of the strains), fluconazole (41.2%), ketoconazole (29.4%), and miconazole (11.8%). No antagonistic effects were observed. The combination of terbinafine plus caspofungin or terbinafine plus fluconazole may have significant therapeutic potential for treatment of pythiosis.
Assuntos
Antifúngicos/farmacologia , Azóis/farmacologia , Equinocandinas/farmacologia , Naftalenos/farmacologia , Pythium/efeitos dos fármacos , Animais , Antifúngicos/classificação , Brasil , Caspofungina , Interações Medicamentosas , Sinergismo Farmacológico , Quimioterapia Combinada , Doenças dos Cavalos/microbiologia , Cavalos , Infecções/microbiologia , Lipopeptídeos , Testes de Sensibilidade Microbiana/métodos , Pythium/classificação , Pythium/isolamento & purificação , TerbinafinaRESUMO
The present study investigated the in vitro inhibitory activity of terbinafine, itraconazole, caspofungin, fluvastatin and ibuprofen against 15 isolates of Pythium insidiosum in double and triple combinations and determined in vivo correlations using rabbits with experimental pythiosis. The minimal inhibitory concentration (MIC) was determined in accordance with the Clinical and Laboratory Standards Institute M 38-A2 protocol (2008), and the in vitro interactions were evaluated using a checkerboard microdilution method. For the in vivo study, 20 rabbits inoculated with P. insidiosum zoospores were divided into four groups: group 1 was treated with terbinafine and itraconazole; group 2 was treated with terbinafine, itraconazole and fluvastatin; group 3 was treated with terbinafine and caspofungin; and group 4 was the control group. Combinations of terbinafine with caspofungin or ibuprofen were synergistic for 47% of the isolates, and antagonism was not observed in any of the double combinations. The triple combinations were mostly indifferent, but synergism and antagonism were also observed. In the in vivo study, the histological aspect of the lesions was similar among the groups, but group 2 showed the lowest amount of hyphae and differed significantly from the other groups.
Assuntos
Antifúngicos/farmacologia , Pitiose/tratamento farmacológico , Pythium/efeitos dos fármacos , Animais , Caspofungina , Interações Medicamentosas , Sinergismo Farmacológico , Quimioterapia Combinada , Equinocandinas/farmacologia , Ácidos Graxos Monoinsaturados/farmacologia , Fluvastatina , Ibuprofeno/farmacologia , Indóis/farmacologia , Itraconazol/farmacologia , Lipopeptídeos , Masculino , Testes de Sensibilidade Microbiana , Naftalenos/farmacologia , Coelhos , TerbinafinaRESUMO
Pythium insidiosum is a zoosporic organism which causes pythiosis in humans and animals. This study aimed to report the paradoxical growth of Brazilian P. insidiosum strains when submitted to in vitro susceptibility tests with caspofungin. The growth at concentrations above the minimal inhibitory concentration (MIC) ranged from 16 to 128 µg/ml and it was observed in 50% of the isolates tested. This paradoxical growth in the presence of caspofungin has been observed with Candida and Aspergillus strains, however, the phenomenon involving oomycetes was described here for the first time.
Assuntos
Antifúngicos/farmacologia , Equinocandinas/farmacologia , Doenças dos Cavalos/microbiologia , Pitiose/veterinária , Pythium/efeitos dos fármacos , Animais , Brasil , Caspofungina , Doenças dos Cavalos/tratamento farmacológico , Cavalos , Lipopeptídeos , Testes de Sensibilidade Microbiana/veterinária , Pitiose/tratamento farmacológico , Pitiose/microbiologia , Pythium/crescimento & desenvolvimentoRESUMO
We evaluated the in vitro activities of voriconazole, itraconazole, and terbinafine against 30 clinical isolates of Pythium insidiosum using a checkerboard macrodilution method. The combined activity of terbinafine plus itraconazole or plus voriconazole was synergic against 17% of the strains. Antagonism was not observed.