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1.
Med Mycol ; 51(6): 652-6, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23373445

RESUMO

Candida spp. are an important cause of nosocomial bloodstream infections. Currently, complete identification of yeasts with conventional methods takes several days. We report here the first evaluation of an extraction method associated with the Vitek MS matrix-assisted laser desorption ionization time of flight mass spectrometry for direct identification of Candida species from positive blood cultures. We evaluated this protocol with blood cultures that were inoculated with reference and routine isolates (eight reference strains, 30 patients isolates and six mixed cultures containing two strains of different Candida species), or from patients with candidemia (28 isolates). This method performed extremely well (97% correct identification) with blood cultures of single Candida spp. and significantly reduced the time of diagnosis. Nevertheless, subculture remains indispensable to test fungal resistance and to detect mixed infections.


Assuntos
Candida/química , Candida/classificação , Candidemia/diagnóstico , Técnicas Microbiológicas/métodos , Micologia/métodos , Manejo de Espécimes/métodos , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Sangue/microbiologia , Candida/isolamento & purificação , Humanos , Sensibilidade e Especificidade , Fatores de Tempo
2.
Bull Cancer ; 109(2): 139-150, 2022 Feb.
Artigo em Francês | MEDLINE | ID: mdl-35034787

RESUMO

BACKGROUND: Advanced practice nursing was introduced in France in 2018, in response to health needs. The first advanced practice nurses were graduated since 2019 and were trained in one among four medical areas including oncology and onco-hematology. The purpose of this article is to make an early assessment of the development of the profession of oncology Advanced Practice Nurse in France. METHOD: An exploratory study was conducted. A sample of 44 onco-hematology IPA graduated in 2019 and 2020 was recruited from June 2021 to end of July 2021. The 44 participants completed a questionnaire, by phone interviews or self-administered. RESULTS AND CONCLUSION: The distribution of the 44 participants concerns 12 of the 13 regions of metropolitan France. This profession shows an employability for 86% of the first graduates. These professionals practice in health care institutions and rather in oncology, 71% in the framework of an organizational protocol established with the oncologist. They appear to be well accepted by patients and oncology teams. Further studies on performance and quality indicators will make it possible to evaluate the added value of the oncology Advanced Practice Nurses in the cancer patient's pathway.


Assuntos
Hematologia , Recursos Humanos de Enfermagem/provisão & distribuição , Enfermagem Oncológica , Inquéritos e Questionários/estatística & dados numéricos , Emprego/estatística & dados numéricos , França , Hematologia/educação , Hematologia/organização & administração , Hematologia/estatística & dados numéricos , Humanos , Processo de Enfermagem/estatística & dados numéricos , Enfermagem Oncológica/educação , Enfermagem Oncológica/organização & administração , Enfermagem Oncológica/estatística & dados numéricos
3.
J Chromatogr A ; 1177(2): 243-53, 2008 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-17919646

RESUMO

Thermodynamic analysis of hydrophobic interaction chromatography of amino acid methyl esters showed entropy-driven adsorption, consistent with solvophobic theory, except for phenyl ester on the Toyopearl resins. All esters adsorbed more strongly to the Toyopearl resins, including the polymethacrylate base matrix, than to Butyl Sepharose. Enthalpy changes were more favorable with the former, explaining the retention difference between Toyopearl Butyl and Butyl Sepharose. An enthalpy change versus heat capacity change plot showed Van der Waals interactions predominantly with the resin matrix. Literature data revealed the same effect for dansylamino acids, shown by isothermodynamic temperature analysis to adsorb more entropically than the esters.


Assuntos
Aminoácidos/química , Cromatografia Líquida/métodos , Interações Hidrofóbicas e Hidrofílicas , Modelos Químicos , Ésteres/química , Ligação de Hidrogênio , Polímeros/química , Sefarose/análogos & derivados , Sefarose/química , Solubilidade , Temperatura , Termodinâmica
4.
Bull Cancer ; 105(7-8): 671-678, 2018.
Artigo em Francês | MEDLINE | ID: mdl-30099993

RESUMO

INTRODUCTION: Research has suggested a high level of satisfaction following beauty and well-being care in oncology. We aimed to assess perception of beauty and well-being care in a large sample of patients affected by cancer. METHODS: From June through August, 2017, a physical and online survey was conducted recruiting patients affected by cancer and their relatives. A questionnaire, established in a collaborative manner with healthcare providers and patients, collected general demographic and medical data, awareness and knowledge data, the experience of beauty and well-being care and perceived benefits. RESULTS: At baseline, 1263 people were recruited (online, n=485; others, n=778) with 1254 usable replies. After excluding caregivers (n=88), the analysis was made on 1166 patients (mean age=51.7). This sample included 1080 women (92.6 %) and breast cancer was prominent (n=827; 70.9%). Among the 481 patients, who had received beauty and well-being care, 405 stated them as a relaxing time (84 %) and the average ranking was 8,1/10. Mean number of sessions was 2.8. A combined scheme of beauty and well-being care (individual and collective) and a greater number of sessions were statistically associated with a higher perceived benefit (P=0.02 and P<0.001) and a higher level of recommendation (P=0.039 and P=0.05). DISCUSSION: This large national survey confirms the high level of satisfaction associated with beauty and well-being care. The type and number of sessions seem to be positively correlated with a greater benefit.


Assuntos
Beleza , Técnicas Cosméticas/psicologia , Promoção da Saúde , Neoplasias/psicologia , Satisfação do Paciente , Qualidade de Vida/psicologia , Indústria da Beleza , Neoplasias da Mama/psicologia , Neoplasias da Mama/terapia , Feminino , França , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias/terapia , Inquéritos e Questionários
5.
Mol Cell Biol ; 22(11): 3744-56, 2002 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-11997510

RESUMO

Mona/Gads is a Grb2-related, Src homology 3 (SH3) and SH2 domain-containing adapter protein whose expression is restricted to cells of hematopoietic lineage (i.e., monocytes and T lymphocytes). During monocyte/macrophage differentiation, Mona is induced and interacts with the macrophage colony-stimulating factor receptor, M-CSFR (also called Fms), suggesting that Mona could be involved in developmental signaling downstream of the M-CSFR by recruiting additional signaling proteins to the activated receptor. Our present results identify Mona as a specific partner protein for the DOS/Gab family member Gab3 in monocytic/macrophage development. Mona does not interact with Gab2; however, Gab3 also forms a complex with the Mona-related adapter Grb2. Glutathione S-transferase pull-down experiments demonstrate that the Mona and Gab3 interaction utilizes the carboxy-terminal SH3 domain of Mona and the atypical proline-rich domain of Gab3. Mona is known to interact with the phosphorylated Y697 site of the M-CSFR. The M-CSFR mutation Y697F exhibited qualitative and quantitative abnormalities in receptor and Gab3 tyrosine phosphorylation, and Mona induction was greatly reduced. The Y807F M-CSFR mutation is defective in differentiation signaling, but not growth signaling, and also fails to induce Mona protein expression. During M-CSF-stimulated macrophage differentiation of mouse bone marrow cells, Mona and Gab3 expression is coinduced, these proteins interact, and Mona engages in multimolecular complexes. These data suggest that association of Mona and Gab3 plays a specific role in mediating the M-CSFR differentiation signal.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal , Proteínas de Transporte/genética , Proteínas de Transporte/metabolismo , Macrófagos/citologia , Macrófagos/metabolismo , Monócitos/citologia , Monócitos/metabolismo , Fosfoproteínas/genética , Fosfoproteínas/metabolismo , Sequência de Aminoácidos , Animais , Sítios de Ligação/genética , Proteínas de Transporte/química , Diferenciação Celular , Linhagem Celular , Expressão Gênica , Células-Tronco Hematopoéticas/citologia , Células-Tronco Hematopoéticas/metabolismo , Camundongos , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Fosforilação , Receptor de Fator Estimulador de Colônias de Macrófagos/genética , Receptor de Fator Estimulador de Colônias de Macrófagos/metabolismo , Homologia de Sequência de Aminoácidos , Transdução de Sinais , Domínios de Homologia de src
6.
BMC Immunol ; 3: 15, 2002 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-12398794

RESUMO

BACKGROUND: Macrophages, osteoclasts, dendritic cells, and microglia are highly specialized cells that belong to the mononuclear phagocyte system. Functional and phenotypic heterogeneity within the mononuclear phagocyte system may reveal differentiation plasticity of a common progenitor, but developmental pathways leading to such diversity are still unclear. RESULTS: Mouse bone marrow cells were expanded in vitro in the presence of Flt3-ligand (FL), yielding high numbers of non-adherent cells exhibiting immature monocyte characteristics. Cells expanded for 6 days, 8 days, or 11 days (day 6-FL, day 8-FL, and day 11-FL cells, respectively) exhibited constitutive potential towards macrophage differentiation. In contrast, they showed time-dependent potential towards osteoclast, dendritic, and microglia differentiation that was detected in day 6-, day 8-, and day 11-FL cells, in response to M-CSF and receptor activator of NFkappaB ligand (RANKL), granulocyte-macrophage colony stimulating-factor (GM-CSF) and tumor necrosis factor-alpha (TNFalpha), and glial cell-conditioned medium (GCCM), respectively. Analysis of cell proliferation using the vital dye CFSE revealed homogenous growth in FL-stimulated cultures of bone marrow cells, demonstrating that changes in differential potential did not result from sequential outgrowth of specific precursors. CONCLUSIONS: We propose that macrophages, osteoclasts, dendritic cells, and microglia may arise from expansion of common progenitors undergoing sequential differentiation commitment. This study also emphasizes differentiation plasticity within the mononuclear phagocyte system. Furthermore, selective massive cell production, as shown here, would greatly facilitate investigation of the clinical potential of dendritic cells and microglia.


Assuntos
Células Dendríticas/fisiologia , Macrófagos/fisiologia , Microglia/fisiologia , Osteoclastos/fisiologia , Proteínas Proto-Oncogênicas/biossíntese , Receptores Proteína Tirosina Quinases/biossíntese , Células-Tronco/fisiologia , Animais , Células da Medula Óssea/citologia , Células da Medula Óssea/efeitos dos fármacos , Células da Medula Óssea/fisiologia , Proteínas de Transporte/farmacologia , Diferenciação Celular/efeitos dos fármacos , Diferenciação Celular/fisiologia , Divisão Celular/efeitos dos fármacos , Divisão Celular/fisiologia , Células Cultivadas , Meios de Cultivo Condicionados/farmacologia , Células Dendríticas/citologia , Fator Estimulador de Colônias de Granulócitos e Macrófagos/farmacologia , Fator Estimulador de Colônias de Macrófagos/farmacologia , Macrófagos/citologia , Macrófagos/efeitos dos fármacos , Glicoproteínas de Membrana/farmacologia , Proteínas de Membrana/farmacologia , Camundongos , Camundongos Endogâmicos C57BL , Microglia/citologia , Osteoclastos/citologia , Ligante RANK , Receptor Ativador de Fator Nuclear kappa-B , Células-Tronco/citologia , Células-Tronco/efeitos dos fármacos , Fator de Necrose Tumoral alfa/farmacologia , Tirosina Quinase 3 Semelhante a fms
8.
Platelets ; 13(8): 459-64, 2002 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-12487779

RESUMO

Mona, also called Gads, is a molecular adapter that plays a key role in T-cell and platelet signalling by linking the adaptors Src homology 2 domain-containing leukocyte phosphoprotein of 76 kDa (Slp-76) and linker for activation of T cells (LAT) upon T-cell receptor and collagen receptor activation. Platelets express a specific form of Mona mRNA, called 1B, which is transcribed from a megakaryocyte-specific promoter. Mona 1B mRNA differ from 1A transcripts found in T cells and some myeloid cells only by the 5'UTR. We report here that 1B mRNA expressing cells do not express detectable amounts of Mona protein, in contrast to 1A expressing cells, and we show that 1B 5'UTR contains upstream open reading frames (uORFs). Mutating the corresponding uAUG restored efficient Mona translation, or that of an unrelated ORF. This suggested that Mona protein expression in 1B mRNA expressing cells is tightly controlled at the translational level. Accordingly, Mona protein was not detected in resting platelets. Strikingly, platelet activation by thrombin resulted in the rapid induction of Mona protein expression, suggesting that translation inhibition of 1B mRNA may be relieved in activated platelets.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal , Proteínas de Transporte/genética , Megacariócitos/fisiologia , Fases de Leitura Aberta , Biossíntese de Proteínas , RNA Mensageiro/genética , Regiões 5' não Traduzidas/genética , Sequência de Bases , Proteínas de Transporte/sangue , Células Cultivadas , Primers do DNA , DNA Complementar , Humanos , Dados de Sequência Molecular , Processamento Pós-Transcricional do RNA , Transfecção , Domínios de Homologia de src
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