Effect of the green synthesis of CuO plate-like nanoparticles on their photodegradation and antibacterial activities.

Green synthesis of copper oxide nanoparticles and its effects on photocatalytic dye degradation and antibacterial activities are reported. The synthesis of nanoparticles by green routes provides many advantages over chemical routes, including simplicity, cost-effectiveness, and fast processing route without using any costly or harmful chemicals. Tridax procumbense (coat buttons) plant root extract was used to synthesize copper oxide nanoparticles. The synthesized Tridax procumbense-copper oxide nanoparticles (TP-CuO NPs) were characterized by UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), dynamic light scattering spectroscopy (DLS), and X-ray diffraction (XRD) techniques. The synthesized TP-CuO NPs were applied for photocatalytic dye degradation and antibacterial activity studies. The TP-CuO NPs exhibited a maximum antibacterial activity at 500 µg mL-1 concentration against Staphylococcus aureus and E. coli showing inhibition zones of 7.5 mm and 7.2 mm, respectively. The photocatalytic ability of the TP-CuO was also tested against the textile dye Trypan blue (TB), and showed about 55% degradation after 48 h for 500 µg mL-1 CuO NP concentration, showing a concentration-dependent degradation efficiency. This is the first work on TP-derived CuO nanoparticles and their photocatalytic and antimicrobial applications. Overall, this study supports the superiority of green-synthesized TP-CuO NPs as photocatalytic and antimicrobial agents.

Nano zinc oxide - An alternate zinc supplement for livestock.

AIM: This study was aimed to investigate antimicrobial and cytotoxicity effect of nano ZnO in in vitro for the application of livestock feed supplement. MATERIALS AND METHODS: Nano ZnO was synthesized by wet chemical precipitation method using zinc acetate as a precursor and sodium hydroxide was used for reducing the precursor salt. The properties of synthesized powder were characterized using ultraviolet (UV)-visible spectroscopy, Fourier transform infrared (FTIR), scanning electron microscopy (SEM), and X-ray diffraction (XRD), respectively. In vitro antimicrobial activities were analyzed against the pathogenic bacteria in poultry Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, and Streptococcus aeruginosa. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was conducted to analyze the cytotoxicity effect of nano ZnO. RESULTS: SEM showed a spherical ZnO particle in the range of 70-100 nm. The size of the particle and purity of the sample were confirmed by XRD. The nano-sized ZnO particles exhibited the UV absorption peak at 335 nm. In FTIR spectroscopy, pure ZnO nanoparticles showed stretching vibrations at 4000-5000 cm-1. ZnO nanoparticles exhibited remarkable antibacterial activity against E. coli, S. aureus, K. pneumoniae, and S. aeruginosa bacterial strains. Cell viability was significantly reduced in a dose-dependent manner in the cytotoxicity study. CONCLUSION: From the broad-spectrum antibacterial activity and the lower cytotoxicity observed at the prescribed dose, it is concluded that nano ZnO powder is a potential alternate zinc supplement for livestock.