Remineralization of post-orthodontic white spot lesions with a fluoride varnish and a self-assembling P 11 - 4 peptides: a prospective in-vivo-study.

OBJECTIVE: The objective was to evaluate the remineralization effects of fluoride varnish (Clinpro White varnish), self-assembling peptide (Curodont™ Repair) and their combined use on WSL after orthodontic treatment. MATERIALS AND METHODS: Thirty-two subjects, aged of 10-18 (mean age 13.91 ± 2.92) with 107 post-orthodontic WSL were included in the study. Subjects were divided into four groups as control, tricalcium phosphate (TCP) containing fluoride varnish (Clinpro White varnish) group, self-assembling P11-4 peptides (Curodont™ Repair) group and combined application of the two products. At the beginning, each subjects' caries risk profile was assessed by evaluating diet cariogenicity, plaque index, gingival bleeding index and stimulated salivary flow rate. Before the application of the remineralization agents, WSL baseline demineralization values were determined with QLF Inspektor™ Pro, laser fluorescence using DIAGNOdent and color values were measured by Vita EasyShade. Remineralization data were obtained by measuring ΔF, ΔQ, and lesion area with QLF. The aesthetic improvement after the remineralization process was evaluated with a spectrophotometer at six weeks, three and six months. RESULTS: No statistically significant differences were found between the groups in terms of criteria determining patients' caries risk profiles, DIAGNOdent data, and plaque index scores (p > 0.05). Intra-group evaluation following remineralization revealed statistically significant increases in ΔF and ΔQ with a decrease in lesion area for the fluoride varnish group at six months, for the peptide group at three months, and for the combined application group at three and six months (p < 0.05). In inter-group comparisons, ΔF and ΔQ values were found to be statistically significant only in the fluoride group at six months compared to the other groups (p < 0.05). While the L* value decreased significantly in all groups at six months, a statistically significant difference in ΔE* values was observed only in the control group between three and six months. CONCLUSION: Fluoride varnish with TCP showed highest remineralization at 6 months, and the remineralization was positively affected in the short term (three months) after the use of self-assembling P11-4 peptides and their combined application. CLINICAL RELEVANCE: Remineralization obtained after single application of agents tested in six months in-vivo showed parallel results. In an attempt to trigger subsurface remineralization, the combined use of fluoride with self-assembling peptides as biomimetic remineralization agent needs further evaluation.

Margin Integrity of Conservative Composite Restorations after Resin Infiltration of Demineralized Enamel.

PURPOSE: To investigate the influence of pretreating demineralized enamel with a caries infiltrant on the margin integrity of Class V composite restorations bonded with different adhesives. MATERIALS AND METHODS: A total of 60 specimens from bovine incisors were demineralized (21 days, acid buffer, pH 4.95) to create artificial enamel lesions, and circular Class V cavities were prepared. Cavities of half of the specimens were treated with either an unfilled etch-and-rinse adhesive (Syntac Classic; Ivoclar Vivadent), a filled etch-and-rinse adhesive (Optibond FL; Kerr), or a self-etch adhesive (iBond Self Etch; Heraeus Kulzer) (n = 10 per group). Demineralized enamel of the other half of the specimens was pretreated with a caries infiltrant (Icon; DMG) prior to adhesive application. All cavities were restored with a nanofilled composite material and thermocycled (5000×, 5°C-55°C). Margin integrity was evaluated using scanning electron microscopy, and the percentage of continuous margin was statistically analyzed (p < 0.05). RESULTS: The significantly highest margin integrity was observed for Optibond FL, whether or not demineralized enamel was pretreated with the infiltrant. Pretreatment of demineralized enamel with the infiltrant resulted in a significant increase in margin integrity when the unfilled etch-and-rinse adhesive (Syntac Classic) or the self-etch adhesive (iBond Self Etch) was subsequently applied, but showed no significant improvement in combination with the filled etch-and-rinse adhesive (Optibond FL). CONCLUSION: Application of a caries infiltrant can improve margin integrity of composite fillings in demineralized enamel when used in combination with the examined self-etch and unfilled adhesives.

In vitro evaluation of the erosive potential of viscosity-modified soft acidic drinks on enamel.

OBJECTIVE: The objective of this in vitro study was to investigate the effect of viscosity-modified soft acidic drinks on enamel erosion. MATERIALS AND METHODS: A total of 108 bovine enamel samples (∅ = 3 mm) were embedded in acrylic resin and allocated into six groups (n = 18). Soft acidic drinks (orange juice, Coca-Cola, Sprite) were used both in their regular forms and at a kinetic viscositiy of 5 mm(2)/s, which was adjusted by adding hydroxypropyl cellulose. All solutions were pumped over the enamel surface from a reservoir with a drop rate of 3 ml/min. Each specimen was eroded for 10 min at 20 °C. Erosion of enamel surfaces was measured using profilometry. Data were analyzed using independent t tests and one-way ANOVAs (p < 0.05). RESULTS: Enamel loss was significantly higher for the regular (Coca-Cola, 5.60 ± 1.04 µm; Sprite, 5.49 ± 0.94 µm; orange juice, 1.35 ± 0.4 µm) than for the viscosity-modified drinks (Coca-Cola, 4.90 ± 0.34 µm; Sprite, 4.46 ± 0.39 µm; orange juice, 1.10 ± 0.22 µm). CONCLUSION: For both regular and viscosity-modified forms, Coca-Cola and Sprite caused higher enamel loss than orange juice. Increasing the viscosity of acidic soft drinks to 5 mm(2)/s reduced enamel erosion by 12.6-18.7 %. CLINICAL RELEVANCE: The erosive potential of soft acidic drinks is not only dependent on various chemical properties but also on the viscosity of the acidic solution and can be reduced by viscosity modification.

Stability of two resin combinations used as sealants against toothbrush abrasion and acid challenge in vitro.

OBJECTIVE: To test the stability of two conventional adhesives when combined with a low-viscosity caries infiltrant used for sealing sound enamel against toothbrush abrasion and acid challenge in vitro. MATERIALS AND METHODS: Bovine enamel discs (Ø = 3 mm) randomly assigned to three groups (n = 10/group) were etched with 37% phosphoric acid for 30 s and treated with resins of different monomer contents forming three test groups: (1) Untreated specimens (Control); (2) Infiltrant (Icon, DMG) + conventional enamel bonding adhesive (Heliobond, Ivoclar Vivadent); and (3) Infiltrant + conventional orthodontic adhesive (Transbond XT Primer, 3M Unitek). All specimens were immersed in hydrochloric acid (pH 2.6) for up to 9 days, during which they were exposed to 1825 toothbrush-strokes per day. Calcium dissolution was assessed using Arsenazo III method at 24-h intervals. Data were analyzed by Kruskal-Wallis and Wilcoxon signed ranks tests. RESULTS: Cumulative calcium dissolution for the untreated specimens (39.75 ± 7.32 µmol/ml) exceeded the sealed groups (Icon + Heliobond: 23.44 ± 7.03 µmol/ml; Icon + Transbond XT Primer: 22.17 ± 5.34 µmol/ml). Untreated specimens presented a relatively constant calcium dissolution rate throughout the experimental period, whereas the sealed groups presented a gradual increase indicating weakening of the seal by toothbrush abrasion. Both sealed groups presented significantly lower daily calcium dissolution at all time points compared to the control, except for Group 2 on the last measurement day. CONCLUSIONS: Low-viscosity caries infiltrant application on sound enamel prior to conventional resin application provided a protective effect against enamel demineralization, but this effect was not stable when challenged mechanically by toothbrush abrasion.

Colour improvement and stability of white spot lesions following infiltration, micro-abrasion, or fluoride treatments in vitro.

SUMMARY BACKGROUND/OBJECTIVES: White spot lesions (WSLs) are unwelcome side effects of fixed appliances that compromise the treatment outcome. Recently, infiltration of WSLs has been introduced as a viable treatment alternative. The objective was to evaluate the colour improvement of WSLs and their stability against discolouration following infiltration, fluoride, or micro-abrasion treatments in vitro. MATERIALS/METHODS: Artificial WSLs were created in bovine enamel (N = 96) using acidic buffer solution (pH 5, 10 days) and were randomly allocated to four groups. Specimens were treated with infiltration (Icon, DMG), fluoride (Elmex Caries Protection, GABA), and micro-abrasion (Opalustre, Ultradent) or remained untreated (control). Groups were discoloured for 24 hours in tea or tea + citric acid. Colour components and visible colour change (L*, a*, b*, ΔE) were measured spectrophotometrically on following time points: baseline, after WSL formation, after treatment, and during discolouration (8, 16, and 24 hours). Data were analysed using Kruskal-Wallis and Mann-Whitney tests. RESULTS: WSL formation increased (L*) in all groups. Only infiltration reduced this effect to baseline. Highest ΔE improvement was obtained by infiltration and micro-abrasion followed by fluoride. This improvement was stable only for infiltration during discolouration. L*, a*, and b* changed significantly during discolouration in all groups except infiltration. Within the same treatment group, discolouration solutions did not differ significantly. LIMITATIONS: In vitro testing cannot replicate the actual mode of colour improvement or stability but can be used for ranking materials and techniques. CONCLUSIONS/IMPLICATIONS: Infiltration and micro-abrasion treatments were capable of diminishing the whitish appearance of WSLs. Only infiltrated WSLs were stable following discolouration challenge.

Effect of chlorhexidine varnish application on streptoococcus mutans colonisation in adolescents with fixed orthodontic appliances.

OBJECTIVE: The aim of this prospective study was to evaluate the re-colonisation pattern of Streptococcus mutans (MS) in highlevel MS-colonised patients with fixed orthodontic appliances following 40% chlorhexidine varnish application prior to bracket placement. MATERIALS AND METHODS: The subjects of this single-blinded clinical trial were 13-14year-old adolescents (N = 14) with significant orthodontic treatment need, a high salivary MS count but without any carious lesions. Baseline MS levels were determined by the cultivation of saliva collected from each subject using strips developed for this purpose (Strip-mutans, Orion Diagnostica, Espoo, Finland). Prior to the bonding of orthodontic brackets, 40% chlorhexidine varnish (EC 40, Explore, Nijmegen, Netherlands) was applied to all teeth for 10 minutes. The re-colonisation of MS was assessed at one, two, four and six week time periods. The data obtained were subjected to a repeated measures design. RESULTS: Chlorhexidine varnish reduced salivary MS significantly at the first, second and fourth weeks compared to baseline values. Significant MS suppression lasted less than six weeks and MS colonisation gradually returned to baseline level. CONCLUSION: Repeated application of chlorhexidine varnish in orthodontic patients with high MS levels may be beneficial throughout fixed appliance orthodontic treatment.

Effect of Caries Infiltrant on Margin Integrity of Composite Fillings Placed Adjacent to Demineralised Primary Enamel.

PURPOSE: To investigate the influence of pretreating demineralised enamel with an infiltrant on the margin integrity of Class V like composite restorations on primary teeth bonded with different adhesives. MATERIALS AND METHODS: Forty specimens from primary molars were demineralised and circular class-V-like cavities were prepared. The cavities were treated with a universal adhesive (Scotchbond Universal Adhesive, 3M Oral Care), applied either in self-etch (SE) or etch-and-rinse mode (ER) mode. In groups SE-I and ER-I, the demineralised margins were pretreated with a caries infiltrant (Icon, DMG) prior to adhesive application. The cavities were restored with a nanofilled composite material and thermocycled. Marginal integrity was evaluated using SEM, and the percentage of continuous margin was statistically analysed. RESULTS: Specimens treated with the caries infiltrant followed by the adhesive showed similar marginal continuity as the adhesive alone. CONCLUSIONS: Pretreatment of demineralised primary enamel with a caries infiltrant before applying a universal adhesive does not influence the marginal integrity of composite fillings.

Comparative evaluation of fluoride varnishes, self-assembling peptide-based remineralization agent, and enamel matrix protein derivative on artificial enamel remineralization in vitro.

BACKGROUND: One of the most unfavorable side effects of fixed orthodontic treatment is white spot lesions (WSLs). Although the most important approach is prevention of WSLs, it is also essential to evaluate the efficacy of the remineralization agents. However, there is no concurrence in the literature with respect to the remineralization process of these agents. The objective of the present study was to evaluate the effects of different fluoride varnishes, enamel matrix protein, and self-assembling peptide derivatives with varying chemical compositions on remineralization of artificially created WSLs in vitro using quantitative light-induced fluorescence (QLF). METHODS: Artificial WSLs were created on bovine enamel samples using acidic buffer solution (pH 5, 10 days). Specimens were randomly allocated to six groups (n = 10/group): (1) Emdogain (Straumann, Basel, Switzerland), (2) Curodont Repair (Credentis AG, Switzerland), (3) Duraphat (Colgate-Palmolive, New York, NY), (4) Clinpro XT (3 M ESPE, Pymble, New South Wales, Australia), (5) Enamel Pro Varnish (Premier Dental Products, PA, USA), and (6) control (untreated). The agents were applied to the WSLs according to the manufacturers' instructions. Fluorescence loss (ΔF), lesion area (area), and impact (ΔQ) values of enamel surfaces were quantified by QLF-D BiluminatorTM (Inspektor-Pro, Amsterdam, The Netherlands) at baseline and after 7, 14, and 21 days of application of the respective materials. RESULTS: ΔF value presented a significantly decreasing trend throughout the 21 days for all groups except the Duraphat and Enamel Pro varnishes. The changes between 14th and 21st days of the Clinpro XT varnish application were significantly higher than Emdogain, Curodont, and Enamel Pro. The Curodont group showed higher lesion area changes between the first and second week in comparison to the Emdogain, Clinpro XT, and Enamel Pro groups, whereas Clinpro XT assured the highest reduction from the second to the third week of the observation period. CONCLUSIONS: The fluorescence loss was significantly reduced with enamel matrix protein, self-assembling peptide, and light-curable fluoride varnishes in the analysis for 21 days. Curodont and Clinpro XT were effective in diminishing the fluorescence loss and lesion area compared to the Duraphat, Enamel Pro fluoride varnishes, and Emdogain in different time points.

Low-Viscosity Resin Infiltration Efficacy on Postorthodontic White Spot Lesions: A Quantitative Light-Induced Fluorescence Evaluation.

OBJECTIVE: The aim of this in vivo study was to evaluate the efficacy of low-viscosity light-cured resin infiltration on postorthodontic white spot lesions (WSLs) on incipient and advanced lesions using quantitative light-induced fluorescence (QLF). METHODS: The study subjects were patients with clinically diagnosed postorthodontic WSLs (n=57). QLF images of the lesions were obtained using a QLF device (Inspektor-Pro, Amsterdam, The Netherlands) before any treatment. Images were processed using the built-in software (QLF patient v2.0.0.48), which produced fluorescence loss (ΔF1), lesion area (Area1), and impact (ΔF1 x Area1, ΔQ1) values. Lesions were categorized as incipient (-5<ΔF1<-12, n=14) or advanced (-12<ΔF1<-25, n=43). They were then infiltrated with low-viscosity resin (Icon-DMG, Hamburg, Germany) according to the manufacturer's instructions. QLF imaging was repeated (ΔF2, Area2, and ΔQ2) from the same aspects assured by the relative software. Kolmogorov-Smirnov, Wilcoxon, and Mann-Whitney tests were used for data evaluation. RESULTS: ΔF1 (-8.40±0.73) and Area1 (3.44±5.19) decreased to -6.58±0.88 and 0.18±0.33 for incipient lesions (p<0.001 and p=0.002, respectively). ΔF1 (-13.20±5.32) and Area1 (4.71±5.56) decreased to -7.51±2.7 and 0.29±1.86 for advanced lesions (p<0.001). When ΔF, lesion area, and ΔQ changes between the groups were compared, the decrease in ΔF was greater for advanced lesions (p<0.001), whereas the decrease in the lesion area and ΔQ was similar (p=0.690, p=0.291). CONCLUSIONS: Infiltration treatment provides improvement of WSLs in terms of fluorescence loss, lesion area, and impact for both incipient and advanced lesions, with the latter group presenting higher fluorescence loss reduction.

Digital image color analysis compared to direct dental CIE colorimeter assessment under different ambient conditions.

PURPOSES: To evaluate the concordance and repeatability of two in vivo methods for dental color assessment and to clarify the influence of different ambient light conditions and subject's head position on the assessed color variables. METHODS: Color assessments were performed by two examiners on 16 arbitrarily selected subjects under two different, standardized conditions of illumination and at two different standardized head angulations. CIE (L*a*b*) data for upper and lower central incisors were recorded in two different ways: (1) by an intra-oral contact dental colorimeter and (2) by processing digital images for performing color calculation using Adobe Photoshop software. The influence of the different ambient conditions on both methods, as well as the concordance of measurements was analyzed statistically using several mixed linear models. RESULTS: Ambient light as a single factor had no significant influence on maxillary L*, a* and b* values, but it did have an effect on mandible assessments. Head angulation variation resulted in significant L* value differences using the photo method. The operator had a significant influence on values a* and b* for the photo method and on a* values for the colorimeter method. In fully lit ambient condition, the operator had a significant influence on the segregated L*, a*, and b* values. With dimmed lights, head angulation became significant, but not the operator. Evaluation of segregated L* values was error prone in both methods. Comparing both methods, deltaE values did not exceed 2.85 units, indicating that color differences between methods and recorded under varying ambient conditions were well below the sensitivity of the naked eye.

Changes in incisor third-order inclination resulting from vertical variation in lingual bracket placement.

OBJECTIVE: To test the null hypothesis that third-order measurements are not correlated to lingual incisor features seen on radiographs. MATERIAL AND METHODS: The lateral headfilms of 38 untreated, norm-occlusion subjects without incisor abrasions or restorations were used for third-order measurements of upper and lower central incisors and assessment of the inclination of four sites suitable for lingual bracket placement with reference to the occlusal plane perpendicular. Lingual sections were determined by the tangents at the incisal fossa (S1), at the transition plateau between incisal fossa and the cingulum (S2), by a constructed line reaching from the incisal tip to the cingulum (S3), and by a tangent at the cingulum convexity (S4). Third-order angles were also assessed on corresponding dental casts using an incisor inclination gauge. Regression analysis was performed using the third-order measurements of both methods as the dependent variables and the inclination of the lingual enamel sections (S1, S2, S3, S4) as the independent variables. RESULTS: The null hypothesis was rejected. For the most common bracket application sites located on the lingual shovel (S1 and S2), third-order inclination changes of 0.4-0.7 degrees are expected for each degree of change in the inclination of the lingual surface. The impact of bracket placement errors on third-order angulation is similar between sections S1 and S2 and the cingulum convexity (S4). Section S3 proved to be least affected by interindividual variation. CONCLUSION: The third-order measurements are correlated to lingual incisor features. Accordingly, third-order changes resulting from variation in lingual bracket placement can be individually predicted from radiographic assessments.

On the interaction between incisor crown-root morphology and third-order angulation.

OBJECTIVE: To evaluate the significance of crown-root angles (CRAs) by testing the null hypothesis that there are no significant differences in deviations of third-order angles to axial inclination values between Angle Class II division 2 incisors and a neutral occlusion control sample. MATERIALS AND METHODS: The study group comprised n(total) = 130 whites with either Angle Class II division 2 (n(1) = 62; group A) or neutral (n(2) = 68; control group B) occlusal relationships. Upper central incisor inclination (U1) was assessed with reference to the cephalometric lines NA and palatal plane (U1NA/deg, U1PP/deg). Craniofacial sagittal and vertical relations were classified using angles SNA, SNB, ANB, and NSL-PP. Third-order angles were derived from corresponding dental cast pairs using an incisor inclination gauge. Welch's two-sample t-tests (alpha-level: .05) were used to test the null hypothesis. Single linear regression was applied to determine third-order angle values as a function of axial inclination values (U1NA, U1PP) or sagittal craniofacial structures (ANB angle), separately for group A and B. RESULTS: The discrepancy between axial inclination (U1NA, U1PP) and third-order angles is significantly different (P < .001) between groups A and B. Regression analysis revealed a simply moderate correlation between third-order measurements and axial inclinations or sagittal craniofacial structures. CONCLUSION: The hypothesis is rejected. The results of this study warn against the use of identical third-order angles irrespective of diminished CRAs typical for Angle Class II division 2 subjects. Routine CRA assessment may be considered in orthodontic treatment planning of Angle Class II division 2 cases.

Systematic evaluation of the features influencing the accuracy of third order measurements.

The aims of this study were to evaluate the relationship of third order measurements on dental casts with those on lateral radiographs, and to identify those incisor features on radiographs which can best explain third order measurements on dental casts. Lateral cephalograms and corresponding dental casts were obtained from 39 untreated Caucasians (12 males, 27 females; mean age 19.5 years; standard deviation 3.7 years) with occlusal relationships considered to be 'normal'. The upper (U1) and lower (L1) incisors were assessed with reference to the occlusal plane perpendicular which was established on the lateral radiographs, including third order angles (U1TR, L1TR) which were also derived from direct dental cast measurements (U1TA, L1TA). Both single regression and multiple linear regression analysis with stepwise variable selection were performed using third order measurements on casts as the dependent variable and crown axis (U1C, L1C), root axis (U1R, L1R), tip-apex connecting line (U1E, L1E), and radiographic third order measurements as independent variables. Single regression analysis indicated an overall difference of 0.02 degrees between radiographic third order inclination and cast assessment in the maxilla (mandible: -2.83 degrees). A change of 1 degree in radiographic third order inclination would produce a change of 0.65 degrees for U1TA and 0.86 degrees for L1TA assessments. Third order measurements on dental casts can best be explained by a linear combination of U1TR and U1E (maxilla) and of L1TR and L1C (mandible) measurements. This study demonstrates the functional enmeshment between two different third order assessments and the most common incisor features on lateral radiographs. Both methods of third order evaluation show sufficient reliability and are appropriate for routine orthodontic practice.

A method for defining targets in contemporary incisor inclination correction.

Different craniofacial properties require individual targets in incisor inclination. These requirements are mostly scheduled on the basis of cephalometric diagnosis, but, however, performed using straightwire appliances, which refer to third-order angles and not to cephalometric data. The objective of this study was to analyze the relationship between incisor third-order angles, incisor inclination, and skeletal craniofacial findings in untreated ideal occlusion subjects with natural dentoalveolar compensation of skeletal variation, in order to link the field of cephalometric assessment of incisor inclination with that of contemporary orthodontic incisor inclination correction. This study utilized lateral cephalograms and corresponding dental casts of 69 untreated Caucasians (21 males and 48 females between 12 and 35 years of age) with neutral (Angle Class I) molar and canine relationships and an incisor relationship that was sagittaly and vertically considered as ideal by three orthodontists (i.e. well supported by the antagonistic teeth and without the need for either deep or open bite correction). Upper (U1) and lower (L1) axial incisor inclinations were assessed with reference to the cephalometric lines NA and NL, and NB and ML, respectively. Sagittal and vertical skeletal relationships were classified using SNA (SNB) and NSL-ML (NSL-NL) angles. Third-order angles (U1TA and L1TA) were derived from direct dental cast measurements using an incisor inclination-recording appliance. The relationships between cephalometric and third-order measurements evaluated by calculating Pearson product-moment correlation coefficients (a = 0.05) showed strong correlations between cephalometric axial inclination data (U1NA/deg, L1NB/deg, U1NA/mm, L1NB/mm, U1NL, and L1ML) and sagittal-skeletal data, but no significant relationship between skeletal-vertical findings and incisor inclination. The mean U1TA was 4.9 (standard deviation [SD] 5.85) and the mean L1TA -3.0 (SD 6.9) degrees. Regression analyses were used for axial inclination (ANB angle designated as the independent variable) and for third-order data (U1NA, L1NB, U1NL, and L1ML designated as independent variables). Based on the correlations found in this study, a novel method for defining targets in upper and lower incisor third-order correction according to natural standards is presented. As a consequence, third-order movements can be adapted to cephalometric diagnosis with enhanced accuracy.

Influence of Lateral Cephalometric Radiographs on Orthodontic Treatment Planning of Class II Patients.

BACKGROUND: Lateral Cephalometric Radiographs (LCR) are a common decision-making aid in orthodontic treatment planning and are routinely used in clinical practice. The aim of this present study was to test the null hypothesis that LCR evaluation does not alter specific components of orthodontic treatment planning in Class II patients. MATERIALS AND METHODS: Records of 75 patients, who had been treated at the Department of Orthodontics, Centre of Dental Medicine, University of Zurich comprised the study material. Inclusion criteria were: (1) adolescents between the age of 12-15, (2) permanent dentition with Class II buccal segment relationship (3) absence of craniofacial and dento-alveolar malformations. Fifteen orthodontists from the dental faculties of Istanbul University, Istanbul and Ege University, Izmir filled out Likert-type linear scale questionnaires without knowing that they would repeat the same procedure with and without LCRs at two different time points. Equivalence and clinical relevance were assessed using (%95 CI) Wilcoxon signed rank tests. RESULTS: Extraction decision did not differ between groups (p=0.68). Preference of functional appliance use (p=0.006) and inter-maxillary fixed functional appliance (p=0.043) was different among groups. CONCLUSION: LCR evaluation has minor influence on treatment planning procedure of Class II patients. It might be beneficial to consider its prescription not in a routine manner but as a supplementary tool considering possible reduction of radiation exposure.

External bleaching effect on the color and luminosity of inactive white-spot lesions after fixed orthodontic appliances.

OBJECTIVE: To evaluate the effect of external bleaching on the color and luminosity of inactive white-spot lesions (WSLs) present after fixed orthodontic appliance treatment as means for achieving color matching of the WSLs with adjacent tooth surfaces. MATERIALS AND METHODS: Ten patients with inactive WSLs after therapy with fixed orthodontic appliances were selected. At baseline, the lightness of maxillary incisors and canines was assessed with a colorimeter. Color determinations were performed in the area of the initial lesions (F1) and at adjacent, sound enamel areas (F2). Then, anterior teeth were bleached once with a bleaching gel for 60 minutes. After a break of 14 days, in-office bleaching was followed by a 2-week home bleaching period with daily home bleaching for 1 hour. After this, color determinations were repeated. Additionally, patients were asked to fill out a questionnaire to provide information about their degree of contentment with the treatment. RESULTS: The lightness values of both the F1 and F2 regions were significantly higher after bleaching as compared with baseline. F2 L-values increased significantly more as compared with F1, indicating a better color matching of these two areas in comparison with baseline. All patients were satisfied with the outcome of the bleaching therapy. CONCLUSION: External bleaching is able to satisfactorily camouflage WSLs visible after therapy with fixed orthodontic appliances.

Cephalometric assessment of the axial inclination of upper and lower incisors in relation to the third-order angle.

BACKGROUND AND AIM: Estimating incisor inclination cephalometrically by reference lines NA and NB puts the orthodontist in the difficult position of relating these axial inclination data to the bracket's third-order prescription which refers to a perpendicular to the occlusal plane. Purpose of the present study was to evaluate the relationship between the cephalometrically-assessed incisor inclination (using the lines NA and NB for reference) and the third-order angle (syn.: torque angle, TA) according to Andrews' description, and moreover to investigate the correlation between incisor inclination data and skeletal-sagittal and skeletal-vertical findings. MATERIALS AND METHODS: The lateral cephalograms and corresponding dental casts of 67 subjects between 10 and 25 years of age (regardless of skeletal and dental relationships) were considered in the study. All subjects were Caucasian, and none had undergone orthodontic therapy. Upper (U1) and lower (L1) incisor angulations were cephalometrically assessed in reference to the NA and NB lines and compared to third-order angles obtained from dental cast measurements with an incisor inclination-recording appliance. Incisor inclination data from the two measurements were correlated to craniofacial sagittal (angles SNA, SNB, ANB) and vertical (angles NSL-NL, NSL-ML, ML-NL) findings from the radiographs. RESULTS: The third-order angles in the upper arch measured on the dental casts were a mean of 16.2 degrees (SD = 5.3 degrees) smaller than the axial inclination according to the NA line; the lower incisor third-order data were less than those of the axial inclination according to the NB line by a mean of 27.8 degrees (SD = 4.75 degrees). In this sample, there was a range of 42.7 degrees for the U1TA variable (mean = 5.6 degrees, SD 9.73 degrees) and 47 degrees for U1NA/ degrees variable (mean = 21.71 degrees, SD = 8.67 degrees). The L1TA variable showed a range of 29 degrees (mean = -2.95 degrees, SD = 7.17 degrees), the radiographic L1NB/ degrees range was 23 degrees (mean = 24.91 degrees, SD = 5.8 degrees). We observed a highly significant correlation (r(NA) = 0.84***, r(NB) = 0.76***) between the Andrews' angle and the inclination estimated in reference to the NA and NB lines. No significant correlation between incisor inclination and craniofacial measurements was detected. CONCLUSIONS: Dental cast measurements seem to be more precise and more valid than lateral radiographs. The method we describe enables clinicians to get a good idea precisely and quickly of how much torque potential remains in the brackets and archwires during treatment. The inclination of the incisors can also be calculated using the regression equations provided, making additional lateral cephalograms unnecessary.

Antimicrobial effectiveness of a highly concentrated chlorhexidine varnish treatment in teenagers with fixed orthodontic appliances.

OBJECTIVE: To evaluate the recolonization pattern of Mutans streptococci (ms) on densely colonized teeth with fixed orthodontic appliances after treatment with a highly concentrated (36%) chlorhexidine varnish. MATERIALS AND METHODS: Healthy subjects (n = 19) with fixed orthodontic appliances and high bacterial ms counts in saliva were recruited. In order to establish a baseline registration, plaque adjacent to brackets was sampled and cultivated on Dentocult strips. Following professional tooth cleaning, chlorhexidine varnish was applied on all teeth for 8 minutes. The degree of recolonization with ms was assessed 2 weeks after varnish application in plaque around the brackets. For statistical analysis, the data were subjected to a repeated measures design. RESULTS: After 2 weeks, ms counts were reduced as compared to baseline values. However, the reduction only weakly met statistical significance (P = .049). CONCLUSIONS: The application of a highly concentrated chlorhexidine varnish in patients with fixed orthodontic appliances does not result in a distinct reduction of ms numbers 2 weeks after treatment.

Influence of Lateral Cephalometric Radiography on Treatment Planning and Preferences in Skeletal Open-Bite Patients: Do Lateral Cephalograms Influence Treatment Planning?

OBJECTIVE: To investigate the influence of diagnostic data derived from lateral cephalometric radiographs (LCR) on treatment preferences of specialists planning skeletal open-bite treatment. METHODS: Diagnostic records of 25 patients who had been treated at the University of Zurich, Department of Orthodontics, between 1988 and 2007 comprised the study material. Inclusion criteria were 1) skeletal open-bite with no marked antero-posterior discrepancy, 2) dental open-bite, and 3) crowding less than 5 mm. Records consisted of extra-intraoral photographs, panoramic-cephalometric X-rays, casts, and results of analyses. Records, with cephalograms of randomly chosen patients removed, were digitally presented to two orthodontists (A and B), and treatment preferences were asked using Likert-type questionnaires. Three months later, the same records were redelivered with missing cephalograms provided and present cephalograms removed with the questionnaire. Data were evaluated for consistency and tendency to extract using Kappa-κ and McNemar tests. RESULTS: Orthodontist B had no poor-agreement scores, whereas orthodontist A presented very-poor agreement for headgear use. Both A (κ=0.833) and B (κ=0.737) had good to very-good agreement in terms of extraction decisions. Neither orthodontist had any significant tendency for extraction/non-extraction therapy (A=0.99; B=0.5). CONCLUSION: Information deduced from LCRs had limited influence on treatment planning preferences in skeletal/dental open-bite patients with no marked antero-posterior discrepancy and no influence on extraction/non-extraction decision.

Erosive effects of different acids on bovine enamel: release of calcium and phosphate in vitro.

The present study intended to investigate minimal erosive effects of different acids on enamel during short time incubation via determination of calcium and phosphate dissolution. Bovine enamel specimens were eroded for 1-5 min with eight different acids of pH 2, 2.3 and 3 (citric (CA), maleic (MA), lactic (LA), tartaric (TA), phosphoric (PA), oxalic (OA), acetic (AA) and hydrochloric acid (HCl)). Calcium (Ca) and phosphate (P) release were determined photometrically using arsenazo III (calcium) and malachite green (phosphate) as substrates. Each subgroup contained eight enamel specimens. Amount of titratable acid was determined for all acidic solutions. MA, LA, TA, AA and HCl caused linear release of Ca and P, PA of Ca, CA of P. For CA, MA, LA, TA, AA, PA and HCl mineral loss was shown to be pH-dependent. Ca dissolution varied between 28.6+/-4.4 (LA, pH 2) and 2.4+/-0.7 nmol mm(-2)min(-1) (HCl, pH 3), P dissolution ranged between 17.2+/-2.6 (LA, pH 2) and 1.4+/-0.4 nmol mm(-2)min(-1) (HCl, pH 3). LA was one of the most erosive acids. AA was very erosive at pH 3. HCl and MA were shown to have the lowest erosive effects. There was only a weak correlation (r=0.28) between P and Ca release and the amount of titratable acid. The method of the present study allows investigation of minimal erosive effects via direct determination of P and Ca dissolution. During short time exposition at constant pH level, erosive effects mainly depend on pH and type of acid but not on amount of titratable acid.