RESUMO
A major challenge in biology is to understand how mechanical interactions and cellular behavior affect the shapes of tissues and embryo morphology. The extension of the neural tube and paraxial mesoderm, which form the spinal cord and musculoskeletal system, respectively, results in the elongated shape of the vertebrate embryonic body. Despite our understanding of how each of these tissues elongates independently of the others, the morphogenetic consequences of their simultaneous growth and mechanical interactions are still unclear. Our study investigates how differential growth, tissue biophysical properties and mechanical interactions affect embryonic morphogenesis during axial extension using a 2D multi-tissue continuum-based mathematical model. Our model captures the dynamics observed in vivo by time-lapse imaging of bird embryos, and reveals the underestimated influence of differential tissue proliferation rates. We confirmed this prediction in quail embryos by showing that decreasing the rate of cell proliferation in the paraxial mesoderm affects long-term tissue dynamics, and shaping of both the paraxial mesoderm and the neighboring neural tube. Overall, our work provides a new theoretical platform upon which to consider the long-term consequences of tissue differential growth and mechanical interactions on morphogenesis.
Assuntos
Proliferação de Células , Mesoderma , Modelos Biológicos , Morfogênese , Tubo Neural , Animais , Mesoderma/embriologia , Mesoderma/citologia , Tubo Neural/embriologia , Tubo Neural/citologia , Codorniz/embriologia , Embrião não Mamífero/citologia , Desenvolvimento Embrionário/fisiologia , ViscosidadeRESUMO
Body axis elongation and segmentation are major morphogenetic events that take place concomitantly during vertebrate embryonic development. Establishment of the final body plan requires tight coordination between these two key processes. In this review, we detail the cellular and molecular as well as the physical processes underlying body axis formation and patterning. We discuss how formation of the anterior region of the body axis differs from that of the posterior region. We describe the developmental mechanism of segmentation and the regulation of body length and segment numbers. We focus mainly on the chicken embryo as a model system. Its accessibility and relatively flat structure allow high-quality time-lapse imaging experiments, which makes it one of the reference models used to study morphogenesis. Additionally, we illustrate conservation and divergence of specific developmental mechanisms by discussing findings in other major embryonic model systems, such as mice, frogs, and zebrafish.
Assuntos
Padronização Corporal , Vertebrados/embriologia , Animais , Desenvolvimento Embrionário , Humanos , Morfogênese , Linha Primitiva , Transdução de Sinais , Vertebrados/metabolismoRESUMO
Embryonic axis elongation is a complex multi-tissue morphogenetic process responsible for the formation of the posterior part of the amniote body. How movements and growth are coordinated between the different posterior tissues (e.g. neural tube, axial and paraxial mesoderm, lateral plate, ectoderm, endoderm) to drive axis morphogenesis remain largely unknown. Here, we use quail embryos to quantify cell behavior and tissue movements during elongation. We quantify the tissue-specific contribution to axis elongation using 3D volumetric techniques, then quantify tissue-specific parameters such as cell density and proliferation. To study cell behavior at a multi-tissue scale, we used high-resolution 4D imaging of transgenic quail embryos expressing fluorescent proteins. We developed specific tracking and image analysis techniques to analyze cell motion and compute tissue deformations in 4D. This analysis reveals extensive sliding between tissues during axis extension. Further quantification of tissue tectonics showed patterns of rotations, contractions and expansions, which are consistent with the multi-tissue behavior observed previously. Our approach defines a quantitative and multi-scale method to analyze the coordination between tissue behaviors during early vertebrate embryo morphogenetic events.
Assuntos
Coturnix/embriologia , Animais , Animais Geneticamente Modificados , Apoptose , Fenômenos Biomecânicos , Padronização Corporal/fisiologia , Contagem de Células , Movimento Celular/fisiologia , Proliferação de Células , Tamanho Celular , Coturnix/genética , Imageamento Tridimensional , Proteínas Luminescentes/genética , Morfogênese/fisiologiaRESUMO
During development, the vertebrate embryo undergoes significant morphological changes which lead to its future body form and functioning organs. One of these noticeable changes is the extension of the body shape along the antero-posterior (A-P) axis. This A-P extension, while taking place in multiple embryonic tissues of the vertebrate body, involves the same basic cellular behaviors: cell proliferation, cell migration (of new progenitors from a posterior stem zone), and cell rearrangements. However, the nature and the relative contribution of these different cellular behaviors to A-P extension appear to vary depending upon the tissue in which they take place and on the stage of embryonic development. By focusing on what is known in the neural and mesodermal tissues of the bird embryo, I review the influences of cellular behaviors in posterior tissue extension. In this context, I discuss how changes in distinct cell behaviors can be coordinated at the tissue level (and between tissues) to synergize, build, and elongate the posterior part of the embryonic body. This multi-tissue framework does not only concern axis elongation, as it could also be generalized to morphogenesis of any developing organs.
Assuntos
Aves/embriologia , Desenvolvimento Embrionário , Animais , Padronização Corporal , Movimento Celular , Proliferação de Células , Humanos , Mesoderma/embriologia , Morfogênese , Vertebrados/embriologiaRESUMO
For over a century, embryologists who studied cellular motion in early amniotes generally assumed that morphogenetic movement reflected migration relative to a static extracellular matrix (ECM) scaffold. However, as we discuss in this Review, recent investigations reveal that the ECM is also moving during morphogenesis. Time-lapse studies show how convective tissue displacement patterns, as visualized by ECM markers, contribute to morphogenesis and organogenesis. Computational image analysis distinguishes between cell-autonomous (active) displacements and convection caused by large-scale (composite) tissue movements. Modern quantification of large-scale 'total' cellular motion and the accompanying ECM motion in the embryo demonstrates that a dynamic ECM is required for generation of the emergent motion patterns that drive amniote morphogenesis.
Assuntos
Matriz Extracelular/metabolismo , Morfogênese , Movimento (Física) , Animais , Humanos , Invertebrados/embriologia , OrganogêneseRESUMO
Embryogenesis is the coordinated assembly of tissues during morphogenesis through changes in individual cell behaviors and collective cell movements. Dynamic imaging, combined with quantitative analysis, is ideal for investigating fundamental questions in developmental biology involving cellular differentiation, growth control and morphogenesis. However, a reliable amniote model system that is amenable to the rigors of extended, high-resolution imaging and cell tracking has been lacking. To address this shortcoming, we produced a novel transgenic quail that ubiquitously expresses nuclear localized monomer cherry fluorescent protein (chFP). We characterize the expression pattern of chFP and provide concrete examples of how Tg(PGK1:H2B-chFP) quail can be used to dynamically image and analyze key morphogenetic events during embryonic stages X to 11.
Assuntos
Animais Geneticamente Modificados , Desenvolvimento Embrionário/fisiologia , Proteínas Luminescentes/metabolismo , Microscopia de Fluorescência/métodos , Modelos Animais , Morfogênese/fisiologia , Imagem com Lapso de Tempo/métodos , Animais , Proliferação de Células/fisiologia , Lentivirus , Plasmídeos/genética , CodornizRESUMO
Vertebrate embryos are characterized by an elongated antero-posterior (AP) body axis, which forms by progressive cell deposition from a posterior growth zone in the embryo. Here, we used tissue ablation in the chicken embryo to demonstrate that the caudal presomitic mesoderm (PSM) has a key role in axis elongation. Using time-lapse microscopy, we analysed the movements of fluorescently labelled cells in the PSM during embryo elongation, which revealed a clear posterior-to-anterior gradient of cell motility and directionality in the PSM. We tracked the movement of the PSM extracellular matrix in parallel with the labelled cells and subtracted the extracellular matrix movement from the global motion of cells. After subtraction, cell motility remained graded but lacked directionality, indicating that the posterior cell movements associated with axis elongation in the PSM are not intrinsic but reflect tissue deformation. The gradient of cell motion along the PSM parallels the fibroblast growth factor (FGF)/mitogen-activated protein kinase (MAPK) gradient, which has been implicated in the control of cell motility in this tissue. Both FGF signalling gain- and loss-of-function experiments lead to disruption of the motility gradient and a slowing down of axis elongation. Furthermore, embryos treated with cell movement inhibitors (blebbistatin or RhoK inhibitor), but not cell cycle inhibitors, show a slower axis elongation rate. We propose that the gradient of random cell motility downstream of FGF signalling in the PSM controls posterior elongation in the amniote embryo. Our data indicate that tissue elongation is an emergent property that arises from the collective regulation of graded, random cell motion rather than by the regulation of directionality of individual cellular movements.
Assuntos
Movimento Celular/fisiologia , Embrião de Galinha/citologia , Embrião de Galinha/embriologia , Fatores de Crescimento de Fibroblastos/metabolismo , Animais , Proliferação de Células , Quimiotaxia , Embrião de Galinha/metabolismo , Neurônios/citologia , Neurônios/metabolismo , Receptores de Fatores de Crescimento de Fibroblastos/genética , Receptores de Fatores de Crescimento de Fibroblastos/metabolismo , Transdução de Sinais , XenopusRESUMO
In the developing ventral spinal cord, motor neurons (MNs) and oligodendrocyte precursor cells (OPCs) are sequentially generated from a common pool of neural progenitors included in the so-called pMN domain characterized by Olig2 expression. Here, we establish that the secreted Sulfatase 1 (Sulf1) is a major component of the mechanism that causes these progenitors to stop producing MNs and change their fate to generate OPCs. We show that specification of OPCs is severely affected in sulf1-deficient mouse embryos. This defect does not rely on abnormal patterning of the spinal cord or failure in maintenance of pMN progenitors at the onset of OPC specification. Instead, the efficiency of OPC induction is reduced, only few Olig2 progenitors are recruited to generate OPCs, meanwhile they continue to produce MNs beyond the normal timing of the neuroglial switch. Using the chicken embryo, we show that Sulf1 activity is required precisely at the stage of the MN-to-OPC fate switch. Finally, we bring arguments supporting the view that Sulf1 controls the level of Sonic Hedgehog (Shh) signaling activity, behaving as an enhancer rather than an obligatory component in the Shh pathway. Our study provides additional insights into the temporal control of Olig2 progenitor cell fate change by the identification of Sulf1 as an extracellular timing signal in the ventral spinal cord.
Assuntos
Diferenciação Celular/fisiologia , Proteínas Hedgehog/metabolismo , Neurônios Motores/citologia , Oligodendroglia/citologia , Medula Espinal/embriologia , Sulfotransferases/metabolismo , Animais , Eletroporação , Imunofluorescência , Hibridização In Situ , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Mutantes , Neurônios Motores/enzimologia , Células-Tronco Neurais/citologia , Células-Tronco Neurais/metabolismo , Oligodendroglia/enzimologia , Transdução de Sinais/fisiologia , Medula Espinal/metabolismoRESUMO
Apoptosis plays an important role in morphogenesis, and the notion that apoptotic cells can impact their surroundings came to light recently. However, how this applies to vertebrate morphogenesis remains unknown. Here, we use the formation of the neural tube to determine how apoptosis contributes to morphogenesis in vertebrates. Neural tube closure defects have been reported when apoptosis is impaired in vertebrates, although the cellular mechanisms involved are unknown. Using avian embryos, we found that apoptotic cells generate an apico-basal force before being extruded from the neuro-epithelium. This force, which relies on a contractile actomyosin cable that extends along the apico-basal axis of the cell, drives nuclear fragmentation and influences the neighboring tissue. Together with the morphological defects observed when apoptosis is prevented, these data strongly suggest that the neuroepithelium keeps track of the mechanical impact of apoptotic cells and that the apoptotic forces, cumulatively, contribute actively to neural tube bending.
Assuntos
Tubo Neural , Neurulação , Animais , Apoptose , Epitélio , MorfogêneseRESUMO
Formation of the primitive streak, the equivalent of the blastopore, is a critical step during the early development of amniote embryos. Medio-lateral cell intercalation and the planar cell polarity pathway play a role during this earliest step of gastrulation in the chick embryo.
Assuntos
Desenvolvimento Embrionário/fisiologia , Linha Primitiva/citologia , Linha Primitiva/fisiologia , Animais , Aves/embriologia , Embrião de Galinha , Drosophila/citologia , Drosophila/embriologia , Mamíferos/embriologiaRESUMO
The neural tube in amniotic embryos forms as a result of two consecutive events along the anteroposterior axis, referred to as primary and secondary neurulation (PN and SN). While PN involves the invagination of a sheet of epithelial cells, SN shapes the caudal neural tube through the mesenchymal-to-epithelial transition (MET) of neuromesodermal progenitors, followed by cavitation of the medullary cord. The technical difficulties in studying SN mainly involve the challenge of labeling and manipulating SN cells in vivo. Here we describe a new method to follow MET during SN in the chick embryo, combining early in ovo chick electroporation with in vivo time-lapse imaging. This procedure allows the cells undergoing SN to be manipulated in order to investigate the MET process, permitting their cell dynamics to be followed in vivo.
Assuntos
Eletroporação/métodos , Células Epiteliais/citologia , Mesoderma/citologia , Neurulação , Imagem com Lapso de Tempo/métodos , Animais , Embrião de Galinha , Células-Tronco Embrionárias/citologia , Células-Tronco Embrionárias/metabolismo , Células Epiteliais/metabolismo , Mesoderma/embriologia , Mesoderma/metabolismoRESUMO
Body axis elongation is a hallmark of the vertebrate embryo, involving the architectural remodeling of the tail bud. Although it is clear how neuromesodermal progenitors (NMPs) contribute to embryo elongation, the dynamic events that lead to de novo lumen formation and that culminate in the formation of a 3-dimensional, neural tube from NMPs, are poorly understood. Here, we used in vivo imaging of the chicken embryo to show that cell intercalation downstream of TGF-ß/SMAD3 signaling is required for secondary neural tube formation. Our analysis describes the events in embryo elongation including lineage restriction, the epithelial-to-mesenchymal transition of NMPs, and the initiation of lumen formation. We show that the resolution of a single, centrally positioned lumen, which occurs through the intercalation of central cells, requires SMAD3/Yes-associated protein (YAP) activity. We anticipate that these findings will be relevant to understand caudal, skin-covered neural tube defects, among the most frequent birth defects detected in humans.
Assuntos
Tubo Neural/metabolismo , Neurulação , Proteína Smad3/metabolismo , Animais , Membrana Basal/metabolismo , Divisão Celular , Linhagem da Célula , Embrião de Galinha , Proteínas de Fluorescência Verde/metabolismo , Imageamento Tridimensional , Mesoderma/citologia , Células-Tronco Neurais/citologia , Imagem com Lapso de Tempo , Fator de Crescimento Transformador beta/metabolismoRESUMO
Although cell-to-cell heterogeneity in gene and protein expression within cell populations has been widely documented, we know little about its biological functions. By studying progenitors of the posterior region of bird embryos, we found that expression levels of transcription factors Sox2 and Bra, respectively involved in neural tube (NT) and mesoderm specification, display a high degree of cell-to-cell heterogeneity. By combining forced expression and downregulation approaches with time-lapse imaging, we demonstrate that Sox2-to-Bra ratio guides progenitor's motility and their ability to stay in or exit the progenitor zone to integrate neural or mesodermal tissues. Indeed, high Bra levels confer high motility that pushes cells to join the paraxial mesoderm, while high levels of Sox2 tend to inhibit cell movement forcing cells to integrate the NT. Mathematical modeling captures the importance of cell motility regulation in this process and further suggests that randomness in Sox2/Bra cell-to-cell distribution favors cell rearrangements and tissue shape conservation.
Assuntos
Diferenciação Celular/genética , Embrião não Mamífero/fisiologia , Proteínas Fetais/genética , Regulação da Expressão Gênica no Desenvolvimento , Expressão Gênica , Fatores de Transcrição SOXB1/genética , Proteínas com Domínio T/genética , Animais , Diferenciação Celular/fisiologia , Linhagem da Célula , CodornizRESUMO
Tissues undergoing morphogenesis impose mechanical effects on one another. How developmental programs adapt to or take advantage of these effects remains poorly explored. Here, using a combination of live imaging, modeling, and microsurgical perturbations, we show that the axial and paraxial tissues in the forming avian embryonic body coordinate their rates of elongation through mechanical interactions. First, a cell motility gradient drives paraxial presomitic mesoderm (PSM) expansion, resulting in compression of the axial neural tube and notochord; second, elongation of axial tissues driven by PSM compression and polarized cell intercalation pushes the caudal progenitor domain posteriorly; finally, the axial push drives the lateral movement of midline PSM cells to maintain PSM growth and cell motility. These interactions form an engine-like positive feedback loop, which sustains a shared elongation rate for coupled tissues. Our results demonstrate a key role of inter-tissue forces in coordinating distinct body axis tissues during their co-elongation.
Assuntos
Embrião não Mamífero/anatomia & histologia , Organogênese , Animais , Fenômenos Biomecânicos , Padronização Corporal , Movimento Celular , Polaridade Celular , Rastreamento de Células , Embrião de Galinha , Simulação por Computador , Mesoderma/embriologia , Codorniz/embriologiaRESUMO
Vertebrates are characterized by an elongated antero-posterior (AP) body axis. This particular shape arises during embryogenesis by mophogenetic events leading to elongation. Although elongation mechanisms that lead to the formation of the anterior part of the body are well described, the ones concerning the posterior part still remain poorly studied. Here, we used tissue ablation in the chicken embryo to demonstrate that caudal presomitic mesoderm (PSM) has a key role in axis elongation. Using time-lapse microscopy, we characterized a clear posterior-to-anterior gradient of cell and tissue motility in the PSM during embryo elongation. Subtracting the tissue movement from the global motion of cells we demonstrated that this gradient correspond to a gradient of cell motility lacking any directionality, indicating that the posterior cell movements associated with axis elongation in the PSM are not intrinsic but reflect tissue deformation. Both FGF signaling gain- and loss-of-function experiments lead to disruption of the motility gradient and a slowing down of axis elongation. Finally we performed experiments indicating that FGF effect on elongation is due to its effect on cell migration and not to regulation of the cell cycle. We propose a new elongation model in which the gradient of non directional cell motility in the PSM controls posterior elongation of the embryo axis.
Assuntos
Movimento Celular , Embrião de Galinha/crescimento & desenvolvimento , Mesoderma/embriologia , Animais , Movimento Celular/efeitos dos fármacos , Movimento Celular/fisiologia , Embrião de Galinha/citologia , Desenvolvimento Embrionário/fisiologia , Fatores de Crescimento de Fibroblastos/farmacologia , Fatores de Crescimento de Fibroblastos/fisiologia , Mesoderma/citologia , Transdução de SinaisRESUMO
Sonic hedgehog (Shh) signaling controls numerous aspects of vertebrate development, including proliferation of precursors in different organs. Identification of molecules that link the Shh pathway to cell cycle machinery is therefore of major importance for an understanding of the mechanisms underlying Shh-dependent proliferation. Here, we show that an actor in the control of entry into mitosis, the phosphatase CDC25B, is transcriptionally upregulated by the Shh/Gli pathway. Unlike other G2/M regulators, CDC25B is highly expressed in domains of Shh activity, including the ventral neural tube and the posterior limb bud. Loss- and gain-of-function experiments reveal that Shh contributes to CDC25B transcriptional activation in the neural tube both of chick and mouse embryos. Moreover, CDC25B transcripts are absent from the posterior limb bud of Shh-/- mice, while anterior grafts of Shh-expressing cells in the chicken limb bud induce ectopic CDC25B expression. Arresting the cell cycle does not reduce the level of CDC25B expression in the neural tube strongly suggesting that the upregulation of CDC25B is not an indirect consequence of the Shh-dependent proliferation. These data reveal an unexpected developmental link between the Shh pathway and a participant in G2/M control.
Assuntos
Proteínas de Ciclo Celular/genética , Divisão Celular/fisiologia , Fase G2/fisiologia , Transativadores/metabolismo , Fosfatases cdc25/genética , Animais , Proliferação de Células , Sistema Nervoso Central/embriologia , Embrião de Galinha , Embrião de Mamíferos , Proteínas Hedgehog , Botões de Extremidades , Camundongos , Camundongos Knockout , Transativadores/fisiologia , Transcrição Gênica , Regulação para CimaRESUMO
In the vertebrate embryo, spinal cord elongation requires FGF signaling that promotes the continuous development of the posterior nervous system by maintaining a stem zone of proliferating neural progenitors. Those escaping the caudal neural stem zone, which is expressed to Shh signal, initiate ventral patterning in the neural groove before starting neuronal differentiation in the neural tube. Here we investigated the integration of D-type cyclins, known to govern cell cycle progression under the control of extracellular signals, in the program of spinal cord maturation. In chicken embryo, we find that cyclin D2 is preferentially expressed in the posterior neural plate, whereas cyclin D1 appears in the neural groove. We demonstrated by loss- and gain-of-function experiments that FGF signaling maintains cyclin D2 in the immature caudal neural epithelium, while Shh activates cyclin D1 in the neural groove. Moreover, forced maintenance of cyclin D1 or D2 in the neural tube favors proliferation at the expense of neuronal differentiation. These results contribute to our understanding of how the cell cycle control can be linked to the patterning programs to influence the balance between proliferation and neuronal differentiation in discrete progenitors domains.
Assuntos
Ciclina D1/metabolismo , Ciclinas/metabolismo , Fatores de Crescimento de Fibroblastos/metabolismo , Medula Espinal/embriologia , Medula Espinal/metabolismo , Transativadores/metabolismo , Animais , Animais Geneticamente Modificados , Sequência de Bases , Padronização Corporal , Ciclo Celular , Diferenciação Celular , Embrião de Galinha , Ciclina D1/genética , Ciclinas/genética , Primers do DNA/genética , Fatores de Crescimento de Fibroblastos/genética , Regulação da Expressão Gênica no Desenvolvimento , Proteínas Hedgehog , Hibridização In Situ , Modelos Neurológicos , Transdução de Sinais , Medula Espinal/citologia , Transativadores/genéticaRESUMO
To address the question of the origin of glial cells and the mechanisms leading to their specification, we have sought to identify novel genes expressed in glial progenitors. We adopted suppression subtractive hybridization (SSH) to establish a chick cDNA library enriched for genes specifically expressed at 6 days of incubation (E6) in the ventral neuroepithelium, a tissue previously shown to contain glial progenitors. Screens were then undertaken to select differentially expressed cDNAs, and out of 82 unique SSH clones, 21 were confirmed to display a regionalized expression along the dorsoventral axis of the E6 ventral neuroepithelium. Among these, we identified a transcript coding for the chick orthologue of Sulf1, a recently identified cell surface sulfatase, as a new, early marker of oligodendrocyte (OL) precursors in the chick embryonic spinal cord. This study provides groundwork for the further identification of genes involved in glial specification.