Widespread learned predator recognition to an alien predator across populations in an amphibian species.

Alien predators are a major cause of decline and extinction of species worldwide, since native organisms are rarely equipped with specific antipredatory strategies to cope with them. However, phenotypic plasticity and learned predator recognition may help prey populations to survive novel predators. Here we examine geographical variation in the learning ability of larval spadefoot toads (Pelobates cultripes) to recognize invasive predatory crayfish (Procambarus clarkii). We compare the learning-mediated behavioural responses of tadpoles from six populations across two regions in Spain (central and southern), with different histories of exposure to the presence of the invasive species. Two of the populations showed innate recognition of chemical cues from the invasive crayfish, whereas three of them learned to recognize such cues as a threat after conditioning with conspecific alarm cues. Learning abilities did not differ among southern populations, but they did among central populations. We assessed patterns of genetic variation within and among these two regions through microsatellite markers and found low genetic divergence among the southern populations but greater differentiation among the central ones. We hypothesize that similar responses to the invasive crayfish in southern populations may have arisen from a combination of extended historical exposure to this introduced predator (~ 50 y) and higher levels of gene flow, as they inhabit a highly interconnected pond network. In contrast, populations from central Spain show lower connectivity, have been exposed to the invasive crayfish for a shorter period of time, and are more divergent in their plastic responses.

The use of the red swamp crayfish (Procambarus clarkii, Girard) as indicator of the bioavailability of heavy metals in environmental monitoring in the River Guadiamar (SW, Spain).

A translocation experiment of red swamp crayfish (Procambarus clarkii) to different sites located in the River Guadiamar was performed in order to assess the ability of this species as bioindicator of heavy metal and metalloid contamination. Crayfish were placed in cages and exposed to polluted environment during either 6 or 12days in the three sites with different concentration of contaminants. Their tissues (exoskeleton+gills, hepatopancreas and abdominal muscle) were dissected and analysed by ICP-MS to assess for concentration of Cd, Cu, Zn, Pb and As. Both exposure times result in significant bioaccumulation of some metals in crayfish tissues as compared to their concentration in the environment. According to overall metal concentration, crayfish tissues rank as follows: hepatopancreas/viscera>exoskeleton/gills>abdominal muscle. Essential metals for crayfish metabolism (Cu and Zn) are always found in high concentrations independently of their quantities in the environment because of the ability of crayfish to manipulate their levels for their own metabolic profit. Metals not involved in crayfish metabolism (Cd, Pb, As) tend to increase with increasing concentration in the surrounding environment and with longer exposure times. Thus crayfish could be used as bioindicator of these pollutants because their dose- and time-dependent accumulation may be reflective of the levels of non-essential metals present in contaminated wetlands. Future guidelines in plans for monitoring contamination on polluted Mediterranean rivers and wetlands should take into account the implementation of the incubation of crayfish during 6days and their subsequent analyses of metal contents, as a routine.