RESUMO
The aim of this study was to describe an outbreak of Shigella sonnei that occurred in the city of Lujan, Buenos Aires, Argentina, in July 2012. Five individuals were affected after eating a hand-made Viennese-style pastry at a family gathering. All of them presented with fever, joint pain, chills and non-bloody diarrhea containing mucus. Stool cultures were performed in all cases and the samples taken from the pastry ingredients were analyzed microbiologically. S.sonnei was isolated and identified in all the patients involved as well as in the almond cream filling. The isolates were analyzed for determining the antimicrobial susceptibility and genetic profiles by pulsed field gel electrophoresis (PFGE). The results showed the genetic relationship among the isolates, confirming that the cases occurred due to the patients' exposure to the same source of infection, i.e., the almond cream. Being the almond cream an industrially-manufactured ingredient, an initial contamination could have been unlikely; however contamination might have occurred as a result of manipulation in the bakery.
Assuntos
Surtos de Doenças , Disenteria Bacilar/epidemiologia , Fast Foods/microbiologia , Microbiologia de Alimentos , Doenças Transmitidas por Alimentos/epidemiologia , Shigella sonnei/isolamento & purificação , Idoso , Antibacterianos/farmacologia , Argentina/epidemiologia , Doces/microbiologia , DNA Bacteriano/genética , Disenteria Bacilar/microbiologia , Eletroforese em Gel de Campo Pulsado , Doenças Transmitidas por Alimentos/microbiologia , Genes Bacterianos , Humanos , Pessoa de Meia-Idade , Polimorfismo de Fragmento de Restrição , Prunus/microbiologia , Shigella sonnei/efeitos dos fármacos , Shigella sonnei/genética , Saúde da População UrbanaRESUMO
El objetivo del estudio fue describir un brote por Shigella sonnei ocurrido en julio de 2012 en Luján, Buenos Aires, Argentina. Estuvieron afectadas 5 personas que asistieron a una reunión familiar, donde consumieron una rosca vienesa de elaboración artesanal adquirida en un comercio. Todos presentaron fiebre, dolores articulares, escalofríos y diarrea no sanguinolenta con mucus. Se realizaron coprocultivos en los afectados y análisis microbiológicos de los ingredientes. Se aisló y caracterizó S.sonnei de todos los pacientes y de la crema de almendras empleada en la preparación de la rosca vienesa. A los aislamientos se les determinó el perfil de sensibilidad a los antimicrobianos y el genético por electroforesis en campo pulsado. Los resultados demostraron la relación genética de los aislamientos, y esto confirmó la ocurrencia de los casos por exposición a una misma fuente de infección, la crema de almendras. Al ser un ingrediente industrial, de improbable contaminación inicial, la crema de almendras podría haber sufrido una contaminación durante la manipulación en la panadería
The aim of this study was to describe an outbreak of Shigella sonnei that occurred in the city of Lujan, Buenos Aires, Argentina, in July 2012. Five individuals were affected after eating a handmade Viennese-style pastry at a family gathering. All of them presented with fever, joint pain, chills and non-bloody diarrhea containing mucus. Stool cultures were performed in all cases and the samples taken from the pastry ingredients were analyzed microbiologically. S.sonnei was isolated and identified in all the patients involved as well as in the almond cream filling. The isolates were analyzed for determining the antimicrobial susceptibility and genetic profiles by pulsed field gel electrophoresis (PFGE). The results showed the genetic relationship among the isolates, confirming that the cases occurred due to the patients' exposure to the same source of infection, i.e., the almond cream. Being the almond cream an industrially-manufactured ingredient, an initial contamination could have been unlikely; however contamination might have occurred as a result of manipulation in the bakery
Assuntos
Humanos , Shigella sonnei/isolamento & purificação , Surtos de Doenças , Infecções/microbiologia , Contaminação de Alimentos/análise , Eletroforese em Gel de Campo Pulsado/métodos , Disenteria Bacilar/diagnóstico , Fezes/microbiologiaRESUMO
Este trabajo muestra las diferencias observadas en las reacciones de inmunidad mediada por células valorada por el método de hinchazón de la pata ("swelling test", RHP) en ratones Balb/c utilizando como antígeno glóbulos rojos de carnero (GRC) procedentes de diferentes animales. Como era de esperar, los resultados de la reacción mostraron que en 9 de los 10 lotes de GRC empleados, los valores con la dosis sensibilizante de 10**6 fueron mayores que los de 10**8. Sin embargo las diferencias tuvieron significación estadística solamente en 3 casos (Cuadro 1). Es de hacer notar que los datos son altamente reproducibles cuando los GRC provienen del mismo animal en diferentes sangrías (Cuadro 2). De acuerdo a estos resultados, la capacidad de desencadenar la RHP está ligada a factores individuales de los animales de los cuales provienen los glóbulos rojos empleados