Genetic diversity among populations of Antarctic springtails (Collembola) within the Mackay Glacier ecotone.

Climate changes are likely to have major influences on the distribution and abundance of Antarctic terrestrial biota. To assess arthropod distribution and diversity within the Ross Sea region, we examined mitochondrial DNA (COI) sequences for three currently recognized species of springtail (Collembola) collected from sites in the vicinity, and to the north of, the Mackay Glacier (77°S). This area acts as a transition between two biogeographic regions (northern and southern Victoria Land). We found populations of highly divergent individuals (5%-11.3% intraspecific sequence divergence) for each of the three putative springtail species, suggesting the possibility of cryptic diversity. Based on molecular clock estimates, these divergent lineages are likely to have been isolated for 3-5 million years. It was during this time that the Western Antarctic Ice Sheet (WAIS) was likely to have completely collapsed, potentially facilitating springtail dispersal via rafting on running waters and open seaways. The reformation of the WAIS would have isolated newly established populations, with subsequent dispersal restricted by glaciers and ice-covered areas. Given the currently limited distributions for these genetically divergent populations, any future changes in species' distributions can be easily tracked through the DNA barcoding of springtails from within the Mackay Glacier ecotone.

The Resilience of Polar Collembola (Springtails) in a Changing Climate.

Assessing the resilience of polar biota to climate change is essential for predicting the effects of changing environmental conditions for ecosystems. Collembola are abundant in terrestrial polar ecosystems and are integral to food-webs and soil nutrient cycling. Using available literature, we consider resistance (genetic diversity; behavioural avoidance and physiological tolerances; biotic interactions) and recovery potential for polar Collembola. Polar Collembola have high levels of genetic diversity, considerable capacity for behavioural avoidance, wide thermal tolerance ranges, physiological plasticity, generalist-opportunistic feeding habits and broad ecological niches. The biggest threats to the ongoing resistance of polar Collembola are increasing levels of dispersal (gene flow), increased mean and extreme temperatures, drought, changing biotic interactions, and the arrival and spread of invasive species. If resistance capacities are insufficient, numerous studies have highlighted that while some species can recover from disturbances quickly, complete community-level recovery is exceedingly slow. Species dwelling deeper in the soil profile may be less able to resist climate change and may not recover in ecologically realistic timescales given the current rate of climate change. Ultimately, diverse communities are more likely to have species or populations that are able to resist or recover from disturbances. While much of the Arctic has comparatively high levels of diversity and phenotypic plasticity; areas of Antarctica have extremely low levels of diversity and are potentially much more vulnerable to climate change.

The Trichoptera barcode initiative: a strategy for generating a species-level Tree of Life.

DNA barcoding was intended as a means to provide species-level identifications through associating DNA sequences from unknown specimens to those from curated reference specimens. Although barcodes were not designed for phylogenetics, they can be beneficial to the completion of the Tree of Life. The barcode database for Trichoptera is relatively comprehensive, with data from every family, approximately two-thirds of the genera, and one-third of the described species. Most Trichoptera, as with most of life's species, have never been subjected to any formal phylogenetic analysis. Here, we present a phylogeny with over 16 000 unique haplotypes as a working hypothesis that can be updated as our estimates improve. We suggest a strategy of implementing constrained tree searches, which allow larger datasets to dictate the backbone phylogeny, while the barcode data fill out the tips of the tree. We also discuss how this phylogeny could be used to focus taxonomic attention on ambiguous species boundaries and hidden biodiversity. We suggest that systematists continue to differentiate between 'Barcode Index Numbers' (BINs) and 'species' that have been formally described. Each has utility, but they are not synonyms. We highlight examples of integrative taxonomy, using both barcodes and morphology for species description.This article is part of the themed issue 'From DNA barcodes to biomes'.