Application of digital holographic tomography in antitumor effect of cantharides complex on 4T1 breast cancer cells.

The study focuses on a methodology providing noninvasive monitoring and evaluation of the antitumor effect of traditional Chinese medicine, cantharides complex (canth), on 4T1 breast tumor cells. Digital holographic tomography (DHT) and developed data post-processing algorithms were used for quantitative estimation of changes in optical and morphological parameters of cells. We calculated and compared data on the refractive index, thickness, and projected area of 4T1 breast tumor cells in control untreated specimens and those treated with doxorubicin hydrochloride (DOX), canth, and their combinations. Post-treatment changes in cellular morphology recorded by DHT demonstrated that the two drugs led to noticeably different morphological changes in cells that can be presumably associated with different pathways of their death, apoptosis, or necrosis. The effect of combined treatment with these two drugs strongly depended on their relative concentrations and could lead to changes characteristic either for DOX or for canth; however, being more profound than those obtained when using each drug solely. The results obtained by DHT are in a good correspondence with commonly used cell viability analysis and immunofluorescent analysis of changes in cellular cytoskeleton.

Effect of object thickness on ultrashort pulse diffraction.

When calculated in the spectral domain, the propagation of an ultrashort optical pulse may suffer from inaccuracy due to the finite thickness of the object it diffracts on. Unlike monochromatic radiation, ultrashort pulse interaction with an object in the time domain depends on the pulse longitudinal coordinate. Here, we propose an algorithm to study the effect of the object thickness on ultrashort pulse diffraction on amplitude, phase, and three-dimensional highly scattering objects. The algorithm comprises a stepwise approach to simulating the diffraction of ultrashort pulses on apertures or scatterers having a finite thickness. We confirm the applicability of the approach and convergence of the result upon reducing the simulation step. We compare the simulation results obtained with traditionally calculated wavefields and the updated results obtained with the proposed approach. We reveal a discrepancy of about 7% for pulsed radiation with λ=800nm on a 1 mm thick object. Then, we demonstrate the dependence of this mismatch on the object thickness and show that for non-Gaussian vortex beams, this effect is even more pronounced. We reveal that spatiotemporal coupling effects depend on the pulse-object interaction simulation approach as well. The obtained results demonstrate that applicability of the single-layer representation of the simulated object strongly depends on its specific features, and inaccuracy of such an approach strongly depends on individual characteristics of the object.

Optimization of DMD-based independent amplitude and phase modulation by analysis of target complex wavefront.

The paper presents the results of a comprehensive study on the optimization of independent amplitude and phase wavefront manipulation which is implemented using a binary digital micromirror device. The study aims to investigate the spatial resolution and quantization achievable using this approach and its optimization based on the parameters of the target complex wave and the modulation error estimation. Based on a statistical analysis of the data, an algorithm for selecting parameters (carrier frequency of binary pattern and aperture for the first diffraction order filtering) that ensures the optimal quality of the modulated wavefront was developed. The algorithm takes into account the type of modulation, that is, amplitude, phase, or amplitude-phase, the size of the encoded distribution, and its requirements for spatial resolution and quantization. The results of the study will greatly contribute to the improvement of modulated wavefront quality in various applications with different requirements for spatial resolution and quantization.

Linear and Nonlinear Elastic Properties of Polystyrene-Based Nanocomposites with Allotropic Carbon Fillers and Binary Mixtures.

We report measurements of linear and nonlinear elastic properties of polystyrene-based nanocomposites with six types of nanofillers, including single and binary mixtures of allotropic carbon nanoparticles. Composite samples were fabricated by the same technology and contained the same filler concentration (5% wt.), which allowed for a direct comparison of their properties. It was shown that the most significant variations of linear and nonlinear elastic properties occur in different nanocomposites. In particular, the most pronounced enhancements of linear elastic moduli (in about 50%) obtained in tensile and flexural tests and in dynamic mechanical analysis were recorded in the sample filled with spherical fullerene nanoparticles. While the most profound rise of absolute values of nonlinear elastic moduli (tens of times) was obtained in the sample filled with the mixture of carbon nanotubes and graphene. The observed tendencies demonstrated the synergistic effect of fillers of different dimensionality on the elastic properties of nanocomposites.

Investigation of Nonlinear Optical Properties of Quantum Dots Deposited onto a Sample Glass Using Time-Resolved Inline Digital Holography.

We report on the application of time-resolved inline digital holography in the study of the nonlinear optical properties of quantum dots deposited onto sample glass. The Fresnel diffraction patterns of the probe pulse due to noncollinear degenerate phase modulation induced by a femtosecond pump pulse were extracted from the set of inline digital holograms and analyzed. The absolute values of the nonlinear refractive index of both the sample glass substrate and the deposited layer of quantum dots were evaluated using the proposed technique. To characterize the inhomogeneous distribution of the samples' nonlinear optical properties, we proposed plotting an optical nonlinearity map calculated as a local standard deviation of the diffraction pattern intensities induced by noncollinear degenerate phase modulation.

Machine Learning Assisted Classification of Cell Lines and Cell States on Quantitative Phase Images.

In this report, we present implementation and validation of machine-learning classifiers for distinguishing between cell types (HeLa, A549, 3T3 cell lines) and states (live, necrosis, apoptosis) based on the analysis of optical parameters derived from cell phase images. Validation of the developed classifier shows the accuracy for distinguishing between the three cell types of about 93% and between different cell states of the same cell line of about 89%. In the field test of the developed algorithm, we demonstrate successful evaluation of the temporal dynamics of relative amounts of live, apoptotic and necrotic cells after photodynamic treatment at different doses.

Polystyrene-Based Nanocomposites with Different Fillers: Fabrication and Mechanical Properties.

The paper presents a comprehensive analysis of the elastic properties of polystyrene-based nanocomposites filled with different types of inclusions: small spherical particles (SiO2 and Al2O3), alumosilicates (montmorillonite, halloysite natural tubules and mica), and carbon nanofillers (carbon black and multi-walled carbon nanotubes). Block samples of composites with different filler concentrations were fabricated by melt technology, and their linear and non-linear elastic properties were studied. The introduction of more rigid particles led to a more profound increase in the elastic modulus of a composite, with the highest rise of about 80% obtained with carbon fillers. Non-linear elastic moduli of composites were shown to be more sensitive to addition of filler particles to the polymer matrix than linear ones. A non-linearity modulus ßs comprising the combination of linear and non-linear elastic moduli of a material demonstrated considerable changes correlating with those of the Young's modulus. The changes in non-linear elasticity of fabricated composites were compared with parameters of bulk non-linear strain waves propagating in them. Variations of wave velocity and decay decrement correlated with the observed enhancement of materials' non-linearity.

Increasing the resolution of the reconstructed image in terahertz pulse time-domain holography.

In this paper, we present a novel numerical approach for increasing the resolution of retrieved images of objects after their diffraction patterns are recorded via terahertz pulse time-domain holography (THz PTDH). THz PTDH allows for spectrally resolved imaging with high spatial resolution and does not require the fine alignment of complex optics in the THz path. The proposed data post-processing method opens up the possibility to reconstruct holograms recorded with spatially restricted THz detectors, and overcome the diffraction limit even for the lower-frequency spectral components. The method involves an iterative procedure of backward-forward wavefront propagation to simulate the field distribution beyond the initially recorded hologram area. We show significant improvement in both the object reconstruction and contrast across the whole spectrum, with qualitative resolution enhancement at lower frequency spectral components.