RESUMO
The ultrastructural localization of succinate dehydrogenase in white rat heart myocytes is studied and heterogeneity of reaction products in separate mitochondria and their groups is described. The enzyme activity is cardiomyocyte electron density dependent. This dependence, in all probability, is the result of different structural and functional states of cells and their organelles, that is revealed by electron microscopy as different electron density of these. It is found that middle electron density cells have the maximum enzyme activity. The mechanisms of enzyme activity dependence of cell electron density are discussed.
Assuntos
Miocárdio/ultraestrutura , Succinato Desidrogenase/metabolismo , Animais , Catálise , Histocitoquímica , Masculino , Microscopia Eletrônica , Miocárdio/enzimologia , Organoides/enzimologia , Organoides/ultraestrutura , RatosRESUMO
Modern notions on the structure, function and localization of thrombin receptors in different human and animal cells are considered. Molecular models of thrombin interaction with receptors are presented. The role these receptors play in the DNA synthesis regulation, proliferation and migration of cells is described. The functions of the thrombin-receptor complexes and mechanisms controlling the quantity of thrombin receptors are discussed.
Assuntos
Receptores de Superfície Celular/metabolismo , Trombina/metabolismo , Animais , Humanos , Modelos Biológicos , Peso Molecular , Receptores de Superfície Celular/fisiologia , Receptores de Trombina , Trombina/fisiologiaRESUMO
Tissue thromboplastin was purified using various chromatographic resins: Sephadex G-200, Ultragel AcA 34, Acrylex P-300 and Toyopearl HW-55F. The purity of the preparations obtained was evaluated by means of the ratio of the thromboplastin solutions absorbency at 540 nm and a protein concentration in the solution expressed as mg/ml. The most purified preparations of tissue thromboplastin were obtained after gel filtration on Toyopearl HW-55F. The thromboplastin preparation produced was used for experimental reproduction of the syndrome of generalized decompensated thrombinogenesis.
Assuntos
Coagulação Intravascular Disseminada/sangue , Tromboplastina/isolamento & purificação , Animais , Testes de Coagulação Sanguínea , Cromatografia em Gel , Coagulação Intravascular Disseminada/patologia , Humanos , Rim/ultraestrutura , Masculino , Miocárdio/ultraestrutura , Ratos , Tromboplastina/fisiologiaRESUMO
Optimum conditions are developed for ultracytochemical detection of cytochrome oxidase in cardiomyocytes from the rat heart ventricles. Conditions mentioned above rest on using of o-dianisidine and double contrasting of microscopic sections by uranyl and lead acetates. Data from studies in heterogeneity of cytochrome oxidase activity as dependent on the structural-functional state (electronic density) of myocardium cells are presented.
Assuntos
Complexo IV da Cadeia de Transporte de Elétrons/metabolismo , Miocárdio/enzimologia , Animais , Catálise , Histocitoquímica , Masculino , Microscopia Eletrônica , Miocárdio/ultraestrutura , Ratos , Coloração e Rotulagem/métodosRESUMO
The impact of titanium (BT-1.0) on proliferative and osteogenic potential of in vitro bone marrow was studied in rabbits. Titanium showed no influence on proliferation and differentiation of osteogenic determined cells precursors in the bone marrow. These cells' growth showed a titanium-tropicity. The authors came to a conclusion that the use of titanium in combination with automyeloplasty can be successfully used to accelerate the bone integration in implanted dental patients.
Assuntos
Medula Óssea/efeitos dos fármacos , Osteogênese/efeitos dos fármacos , Titânio/farmacologia , Animais , Células da Medula Óssea , Divisão Celular/efeitos dos fármacos , Células Cultivadas , Coelhos , Fatores de TempoAssuntos
Proteínas de Transporte/farmacologia , Inibidores de Proteases/farmacologia , Trombina/farmacologia , Precursor de Proteína beta-Amiloide , Proteínas de Transporte/metabolismo , Células Cultivadas , Interações Medicamentosas , Humanos , Lisossomos/efeitos dos fármacos , Lisossomos/metabolismo , Peso Molecular , Inibidores de Proteases/metabolismo , Nexinas de Proteases , Ligação Proteica/efeitos dos fármacos , Receptores de Superfície Celular/efeitos dos fármacos , Receptores de Superfície Celular/metabolismo , Receptores de Trombina , Trombina/antagonistas & inibidores , Trombina/metabolismoRESUMO
In the experiments on 40 pregnant white rats, the authors used electron microscopy for the assessment of structural renal changes in the dams and their fetuses, which had occurred in generalized decompensated thrombogenesis resulted from massive heterohemotransfusion. The common coagulative origin was established for the lesions in dams and fetuses, which appeared as disseminated microthrombosis, degenerated interstitial connective tissue and cell structures.