In vitro effects of CaO nanoparticles on Triticale callus exposed to short and long-term salt stress.

KEY MESSAGE: Ca2+ NPs enhanced tolerance of Triticale callus under salt stress by improving biochemical activity and confocal laser scanning analysis, conferring salt tolerance on callus cells. CaO NPs (Ca2+) are significant components that act as transducers in many adaptive and developmental processes in plants. In this study, effect of Ca2+ NPs on the response and regulation of the protective system in Triticale callus under short and long-salt treatments was investigated. The activation of Ca2+ NPs was induced by salt stress in callus of Triticale cultivars. MDA, H2O2, POD, and protein activities were determined in callus tissues. Concerning MDA, H2O2, protein activities, it was found that the Ca2+ NPs treatment was significant, and it demonstrated a high correlation with the tolerance levels of cultivars. Tatlicak cultivar was detected for better MDA activities in the short time with 1.5 ppm Ca2+ NPs concentration of 50 g and 100 g NaCl. Similarly, the same cultivar responded with better H2O2 activity at 1.5 ppm Ca2+ NPs 100 g NaCl in the short time. POD activities exhibited a decreasing trend in response to the increasing concentrations of Ca2+ NPs. The best result was observed at 1.5 ppm Ca2+ NPs 100 g NaCl in the short term. Based on the protein content, treatment of short-term cultured callus cells with 1.5 ppm Ca2+ NPs inhibited stress response and it significantly promoted Ca2+ NPs signals as compared to control callus. Confocal laser scanning analysis proved that the application of Ca2+ NPs could alleviate the adverse effects of salt stress by the inhibition of stress severity in callus cells. This study demonstrated, under in vitro conditions, that the application of Ca2+ NPs can significantly suppress the adverse effects of salt stress on Triticale callus; it was also verified that the concentration of Ca2+ NPs could be important parameter to be considered in adjusting the micronutrient content in the media for this plant.

Cone-beam computed tomography evaluation of the soft tissue thickness and greater palatine foramen location in the palate.

AIM: The aim of this study was to evaluate the palatal mucosa thickness and greater palatine foramen location using cone-beam computerized tomography (CBCT). MATERIALS AND METHODS: Cone-beam computerized tomography images of 345 patients were measured. The relationship between palate vault depth and angle between the alveolar bone and palatal plane (PA) was evaluated. Differences in the palatal mucosal thickness according to age, gender and tooth site were evaluated. RESULTS: The mean palatal mucosal thickness from the second molar to the canine teeth were 3.7, 3.3, 3.7, 3 and 3 mm, respectively. The palatal mucosal thickness at the second molar and second premolar was statistically different from those at other zones. There was no correlation between the palatal junction angle and the palatal depth. Greater palatine foramen was observed at the level of third molar tooth, between third and second molar, second molar 63%, 31% and 6% in men and 56%, 36% and 8% in women respectively. CONCLUSIONS: The second premolar to second molar zone was considered a suitable graft site based on the mean palatal mucosal thickness.

Relationship between orthodontic treatment and gingival health: A retrospective study.

OBJECTIVE: The aim of this retrospective study was to evaluate the relationship between orthodontic treatment and gingival health. MATERIALS AND METHODS: A total of 251 patients among whom 177 were girls and 74 were boys, recruited from the records pool of the Department of Orthodontics, Faculty of Dentistry, University of Gazi, were included in the study. Patients' treatments have been completed by postgraduate students during the period between 2006 and 2012. Patients' folders were analyzed according to their age, treatment time, and the type of orthodontic treatment. Intra-oral photographs were analyzed, and the presence or absence of visible plaque, visible inflammation, and gingival recession were recorded, and incisor inclinations analyzed on lateral cephalometric films, before and after orthodontic treatment. RESULTS: No statistically significant difference was found in patients treated with functional appliances before and after treatment. In patients treated with fixed orthodontic appliances, visible plaque, visible inflammation, and gingival recession showed significant increases after treatment, gingival biotype did not show any significant difference. Positive correlation was found between lower incisor position and gingival recession in patients treated with fixed appliance and extraction. And also cuspids were the teeth with the highest prevalence of gingival recession. CONCLUSION: Considering the relationship between orthodontic treatment and gingival health, cooperation among patients, orthodontists, and periodontists is important.

Shallow localized gingival recession defects treated with modified coronally repositioned flap technique: A case series.

OBJECTIVES: Various coronally repositioned flap (CRF) techniques have been proposed for coverage of gingival recession defects. Although CRF has several modifications all of them needs vertical or oblique external releasing incisions for treatment of localized gingival recession defects. The aim of present article was to evaluate the effectiveness of a modification of the new CRF procedure without any releasing incision for treatment of shallow localized gingival recession defects. CONCLUSION: Shallow localized gingival recession defects can be treated with modified coronally repositioned flap technique successfully.