1.
Angew Chem Int Ed Engl
; 58(23): 7873-7877, 2019 06 03.
Artigo
em Inglês
| MEDLINE
| ID: mdl-30945422
RESUMO
An increasing number of biocatalytic oxidation reactions rely on H2 O2 as a clean oxidant. The poor robustness of most enzymes towards H2 O2 , however, necessitates more efficient systems for inâ situ H2 O2 generation. In analogy to the well-known formate dehydrogenase to promote NADH-dependent reactions, we here propose employing formate oxidase (FOx) to promote H2 O2 -dependent enzymatic oxidation reactions. Even under non-optimised conditions, high turnover numbers for coupled FOx/peroxygenase catalysis were achieved.
Assuntos
Aspergillus oryzae/enzimologia , Formiatos/metabolismo , Proteínas Fúngicas/metabolismo , Peróxido de Hidrogênio/metabolismo , Oxigenases de Função Mista/metabolismo , Oxirredutases/metabolismo , Oxigênio/metabolismo , Biocatálise , Cinética , Oxirredução
2.
Chem Commun (Camb)
; (18): 1843-5, 2007 May 14.
Artigo
em Inglês
| MEDLINE
| ID: mdl-17476406
RESUMO
A dual function blue fluorescent protein from Vibrio vulnificus is also an NADPH-dependent oxidoreductase, rendering it a useful tool for biophysical studies.