Esthetic considerations for reshaping of autotransplanted premolars replacing maxillary central incisors: a case report.

Autotransplantation is a safe and currently common procedure in cases of agenesis and tooth loss. Autotransplantation of premolars to replace missing maxillary central incisors has been shown to be an excellent treatment choice, especially in young patients in the 9-12-year-old age range. The choice for adequate restorative materials and techniques is also essential, direct composite resin being indicated in most cases. This paper reports the case of two premolars autotransplanted to replace both maxillary central incisors and addresses the esthetic issues involved in the reshaping of these teeth to incisor morphology.

Antimicrobial effect of chlorhexidine digluconate in dentin: In vitro and in situ study.

AIM: The aim of this study was to evaluate a very short-term in vitro and in situ effect of 2% chlorhexidine-digluconate-based (CHX) cavity cleanser on the disinfection of dentin demineralized by cariogenic bacteria. MATERIALS AND METHODS: Human dentin slabs were randomly allocated and used in 2 distinct phases, in vitro and in situ, for obtaining demineralized dentin. In vitro, the slabs (n=15) were immersed for 5 days in BHI broth inoculated with Streptococcus mutans CTT 3440. In situ, a double-blind design was conducted in one phase of 14 days, during which 20 volunteers wore palatal devices containing two human dental dentin slabs. On 5(th) day in vitro and 14(th) day in situ, the slabs were allocated to the two groups: Control group (5 µl of 0.9% NaCl solution) and CHX group (5 µl of 2% chlorhexidine digluconate solution, Cavity Cleanser™ BISCO, Schaumburg, IL, EUA), for 5 minutes. The microbiological analyses were performed immediately before and after the treatments. RESULTS: The log reductions means found for CHX treatment on tested micro organisms were higher when compared to Control group either in vitro or in situ conditions. CONCLUSIONS: Our results showed that CHX was effective in reducing the cultivable microbiota in contaminated dentin. Furthermore, although the use of chlorhexidine-digluconate-based cavity disinfectant did not completely eliminate the viable microorganisms, it served as a suitable agent to disinfect tooth preparations.