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1.
Phys Rev Lett ; 106(8): 085004, 2011 Feb 25.
Artigo em Inglês | MEDLINE | ID: mdl-21405580

RESUMO

We demonstrate the hohlraum radiation temperature and symmetry required for ignition-scale inertial confinement fusion capsule implosions. Cryogenic gas-filled hohlraums with 2.2 mm-diameter capsules are heated with unprecedented laser energies of 1.2 MJ delivered by 192 ultraviolet laser beams on the National Ignition Facility. Laser backscatter measurements show that these hohlraums absorb 87% to 91% of the incident laser power resulting in peak radiation temperatures of T(RAD)=300 eV and a symmetric implosion to a 100 µm diameter hot core.

2.
3.
Gene ; 22(1): 85-93, 1983 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-6305774

RESUMO

pCtBR2-1 is a recombinant plasmid with a 750-bp insert of Chironomus tentans genomic DNA. When pCtBR2-1 was hybridized in situ to salivary gland polytene chromosomes, it hybridized exclusively to Balbiani ring 2 (BR2), a giant chromosomal puff. It was also shown that the insert contained four tandemly repeated sequences that were delineated by HinfI sites which occurred every 190 bp. The purified insert reassociated to C. tentans DNA with a C0t1/2 = 0.48 indicating that the sequence was moderately repeated within the genome. Hybridization of radioactive pCtBR2-1 to nitrocellulose blots containing partial HinfI digests of genomic DNA revealed that the 190-bp repeats were organized into one or more blocks of 11 to 12 copies in tandem. Hybridization of the recombinant plasmid to limit digests of genomic DNA also demonstrated that repeated sequences in BR2 were not homogeneous. As much as 70% of BR2 appeared to be represented by a 26-kb HhaI-resistant core, while the remaining 30% may have HhaI sites at 190-bp intervals, similar to pCtBR2-1.


Assuntos
Chironomidae/genética , DNA Recombinante , Dípteros/genética , Sequências Repetitivas de Ácido Nucleico , Animais , Mapeamento Cromossômico , Cromossomos/ultraestrutura , Clonagem Molecular , Enzimas de Restrição do DNA , Ácidos Nucleicos Heteroduplexes/genética , Hibridização de Ácido Nucleico , Plasmídeos , Glândulas Salivares/ultraestrutura
5.
J Biol Chem ; 260(21): 11824-30, 1985 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-2413016

RESUMO

A second gene has been discovered at a previously studied Balbiani ring in Chironomus. Northern hybridizations demonstrated that cDNA clone pCt35 originated from a salivary gland specific 6.5-kilobase (kb) RNA that was abundant, nonribosomal, and apparently poly(A)+. pCt35 had a 120-base pair (bp) insert with 1.6 copies of a 75-bp sequence that contained two open reading frames. Southern hybridizations indicated that pCt35 was homologous to at least a 4-kb block of genomic DNA organized as a hierarchy of 150- and 300-bp tandem repeats. In situ hybridization localized these sequences to Balbiani ring 1. From these results we postulated that a 6.5-kb RNA gene may have evolved by stepwise duplication and amplification of a 75-bp ancestral sequence.


Assuntos
Chironomidae/genética , Dípteros/genética , Genes , Poli A/análise , RNA/análise , Animais , Evolução Biológica , Sequências Repetitivas de Ácido Nucleico , Glândulas Salivares/análise , Homologia de Sequência do Ácido Nucleico , Transcrição Gênica
6.
J Infect Dis ; 160(2): 243-7, 1989 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-2668422

RESUMO

Five infants from a day care center developed severe diarrhea associated with enteropathogenic Escherichia coli O114:nonmotile (EPEC O114:NM) and required hospitalization. Five additional cases of diarrhea associated with EPEC O114:NM subsequently occurred, four in hospital contacts of the patients and one in a household contact. Biochemically, all EPEC O114:NM isolates were sorbitol nonfermenters. All isolates produced low concentrations of cytotoxin with a mean of 10(1.23) CD50/mg of protein. Cytotoxin was not neutralized with antibody to Shiga-like toxin I or II. Heat-labile and heat-stable enterotoxins were not present by gene probe analysis. Stool isolates from 9 of 10 hospitalized infants were positive for EPEC adherence factor by colony blot DNA probe analysis. The severity of the disease, sorbitol nonfermentation, and presence of enteroadherence are unusual features of this organism.


Assuntos
Diarreia/epidemiologia , Surtos de Doenças , Infecções por Escherichia coli/epidemiologia , Creches , Escherichia coli/isolamento & purificação , Feminino , Humanos , Lactente , Masculino , Testes de Neutralização
7.
J Virol ; 73(5): 3843-53, 1999 May.
Artigo em Inglês | MEDLINE | ID: mdl-10196279

RESUMO

Two intrastrain variants of herpes simplex virus type 1 (HSV-1) were isolated from a newborn with fatal disseminated infection. A small-plaque-producing variant (SP7) was the predominant virus (>99%) in the brain, and a large-plaque-producing variant (LP5) was the predominant virus (>99%) in the lung and gastrointestinal tract. EcoRI and BamHI restriction fragment patterns indicated that SP7 and LP5 are related strains. The large-plaque variants produced plaques similar in size to those produced by HSV-1 KOS. Unlike LP5 or KOS, SP7 was highly cell associated and processing of glycoprotein C and glycoprotein D was limited to precursor forms in infected Vero cells. The large-plaque phenotype from KOS could be transferred into SP7 by cotransfection of plasmids containing the EK or JK EcoRI fragment or a 3-kb plasmid with the UL34.5 gene of HSV-1 KOS together with SP7 DNA. PCR analysis using primers from within the ICP34.5 gene indicated differences for SP7, LP5, and KOS. Sequencing data indicated two sets of deletions in the UL34.5 gene that distinguish SP7 from LP5. Both SP7 and LP5 variants were neurovirulent (lethal following intracranial inoculation of young BALB/c mice); however, the LP5 variant was much less able to cause lethal neuroinvasive disease (footpad inoculation) whereas KOS caused no disease. Passage of SP7 selected for viruses (SLP-5 and SLP-10) which were attenuated for lethal neuroinvasive disease, were not cell-associated, and differed in the UL34.5 gene. UL34.5 from SLP-5 or SLP-10 resembled that of KOS. These findings support a role for UL34.5 in promoting virus egress and for neuroinvasive disease.


Assuntos
Variação Genética , Herpesvirus Humano 1/genética , Processamento de Proteína Pós-Traducional , Proteínas Virais/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , DNA Viral , Desoxirribonuclease BamHI , Desoxirribonuclease EcoRI , Modelos Animais de Doenças , Genes Virais , Glicoproteínas/metabolismo , Herpesvirus Humano 1/crescimento & desenvolvimento , Herpesvirus Humano 1/isolamento & purificação , Humanos , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Dados de Sequência Molecular , Reação em Cadeia da Polimerase/métodos , Polimorfismo de Fragmento de Restrição , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos , Homologia de Sequência do Ácido Nucleico , Proteínas do Envelope Viral/análise
8.
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