RESUMO
There is a significant gap in our knowledge of the microbe-host relationship between urban and traditional rural populations. We conducted a large-scale study to examine the gut microbiota of different traditional rural and urban lifestyles in human populations. Using high-throughput 16S ribosomal RNA gene amplicon sequencing, we tested urban French, Saudi, Senegalese, Nigerian and Polynesian individuals as well as individuals living in traditional rural societies, including Amazonians from French Guiana, Congolese Pygmies, Saudi Bedouins and Algerian Tuaregs. The gut microbiota from individuals living in traditional rural settings clustered differently and presented significantly higher diversity than those of urban populations (p 0.01). The bacterial taxa identified by class analysis as contributing most significantly to each cluster were Phascolarctobacterium for traditional rural individuals and Bifidobacterium for urban individuals. Spirochaetae were only present in the gut microbiota of individuals from traditional rural societies, and the gut microbiota of all traditional rural populations was enriched with Treponema succinifaciens. Cross-transmission of Treponema from termites or swine to humans or the increased use of antibiotics in nontraditional populations may explain why Treponema is present only in the gut microbiota of traditional rural populations.
RESUMO
Culturomics investigates microbial diversity of the human microbiome by combining diversified culture conditions, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and 16S rRNA gene identification. The present study allowed identification of four putative new Clostridium sensu stricto species: 'Clostridium amazonitimonense' strain LF2T, 'Clostridium massilidielmoense' strain MT26T, 'Clostridium nigeriense' strain Marseille-P2414T and 'Clostridium merdae' strain Marseille-P2953T, which we describe using the concept of taxonogenomics. We describe the main characteristics of each bacterium and present their complete genome sequence and annotation.
RESUMO
INTRODUCTION: Geophagy or soil eating is mostly described in pregnant women from Sub-Saharan Africa, South America. Here, we report 12 cases of geophagy associated with severe anemia in non-pregnant Nigerian women. RESULTS/CASE REPORTS: The median age at diagnosis was 34.5 years. The socioeconomic level was average for all patients. The median hemoglobin level at admission was 6.9g/dL (3.3-8.6), median corpuscular volume was 78.3fL (63-106) and median serum ferritin was 9.2ng/mL (3.6-11.2). The reasons of this practice were "desire" (5/12) and tradition (4/12). All patients received psychotherapy and supplementation with intravenous iron. CONCLUSION: Geophagy is an underestimated practice in developed countries and in non-pregnant women. It can be the cause of severe iron deficiency and must be discussed in patients with anemia, including non-pregnant patients, and in Africa as well as in migration areas, where the practice can be exported.
Assuntos
Anemia/complicações , Pica/etiologia , Adolescente , Adulto , Anemia/epidemiologia , Estudos de Coortes , Feminino , Hospitalização/estatística & dados numéricos , Humanos , Pica/epidemiologia , Índice de Gravidade de Doença , Adulto JovemRESUMO
We report here the main characteristics of five new species, 'Marasmitruncus massiliensis' strain Marseille-P3646T (CSUR P3646), 'Clostridium culturomicum' strain Marseille-P3545T (CSUR P3545), 'Blautia provencensis' strain Marseille-P3502T (CSUR P3502), 'Bacillus caccae' strain Marseille-P3604T (CSUR P3604) and 'Ornithinibacillus massiliensis' strain Marseille-P3601T (CSUR P3601), which were isolated recently from undernourished children's stool samples from Niger using microbial culturomics.
RESUMO
The main characteristics of 'Khelaifiella massiliensis' strain Mt13T (= CSUR P1935, = DSM100591), 'Niameybacter massiliensis' strain Mt14T (= CSUR P1909, = DSM100592), 'Brachybacterium massiliense' strain MT5T (= CSUR P2240, = DSM101766), 'Enterobacter timonensis' strain mt20T (= CSUR P2201, = DSM 101775) and 'Massilibacillus massiliensis' strain Marseille-P2411T (= CSUR P2411, = DSM102838), new species isolated from the gut of healthy African infants, are presented.
RESUMO
Paenibacillus phocaensis sp. nov. strain mt24T (= CSUR P2238 = DSM 101777) is a Gram-negative, facultative anaerobic, spore-forming and motile bacilli. This strain was isolated from the stool sample of a healthy infant from Niger. Its genome was estimated to a size of 5 521 415 bp with a 53.54% GC content. It contains 4835 protein-coding genes and 89 RNAs, among which two were 16S rRNA genes. There were also 101 genes (2.09%) identified as ORFans.
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We report here the main characteristics of five new species 'Urmitella timonensis' strain Marseille-P2918T (CSUR P2918), 'Blautia marasmi' strain Marseille-P2377T (CSUR P2377), 'Lachnoclostridium pacaense' strain Marseille-P3100T (CSUR P3100), 'Bacillus marasmi' strain Marseille-P3556T (CSUR P3556) and 'Anaerotruncus rubiinfantis' strain MT15T (CSUR P2276), which were isolated recently from stool samples taken from undernourished children in Niger and Senegal using microbial culturomics.
RESUMO
Microbial culturomics represents a completely new approach to investigate microbial diversity by using different optimized culture conditions, mass spectrometry, genome sequencing and annotation and phenotypic description that allow for an extensive characterization of new species and the study of the human microbiome. Here we present four new species within the genus Paenibacillus: 'Paenibacillus bouchesdurhonensis' strain Marseille-P3071T, 'Paenibacillus rubinfantis' strain MT18T, 'Paenibacillus senegalimassiliensis' strain SIT18T and 'Paenibacillus tuaregi' strain Marseille-P2472T, which are all facultatively aerobic and Gram-positive bacilli.
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A new Actinobacterium, designated Tessaracoccus massiliensis type strain SIT-7(T) (= CSUR P1301 = DSM 29060), have been isolated from a Nigerian child with kwashiorkor. It is a facultative aerobic, Gram positive, rod shaped, non spore-forming, and non motile bacterium. Here, we describe the genomic and phenotypic characteristics of this isolate. Its 3,212,234 bp long genome (1 chromosome, no plasmid) exhibits a G+C content of 67.81% and contains 3,058 protein-coding genes and 49 RNA genes.
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Bacillus andreraoultii strain SIT1(T) (= CSUR P1162 = DSM 29078) is the type strain of B. andreraoultii sp. nov. This bacterium was isolated from the stool of a 2-year-old Nigerian boy with a severe form of kwashiorkor. Bacillus andreraoultii is an aerobic, Gram-positive rod. We describe here the features of this bacterium, together with the complete genome sequencing and annotation. The 4 092 130 bp long genome contains 3718 protein-coding and 116 RNA genes.
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Bacillus rubiinfantis sp. nov. strain mt2(T) is the type strain of B. rubiinfantis sp. nov., isolated from the fecal flora of a child with kwashiorkor in Niger. It is Gram-positive facultative anaerobic rod belonging to the Bacillaceae family. We describe the features of this organism alongside the complete genome sequence and annotation. The 4 311 083 bp long genome (one chromosome but no plasmid) contains 4028 protein-coding gene and 121 RNA genes including nine rRNA genes.
RESUMO
Bacillus niameyensis sp. nov. strain SIT3(T) (= CSUR P1266 = DSM 29725) is the type strain of B. niameyensis sp. nov. This Gram-positive strain was isolated from the digestive flora of a child with kwashiorkor and is a facultative anaerobic rod and a member of the Bacillaceae family. This organism is hereby described alongside its complete genome sequence and annotation. The 4 286 116 bp long genome (one chromosome but no plasmid) contains 4130 protein-coding and 66 RNA genes including five rRNA genes.
RESUMO
Amyotrophic Lateral Sclerosis (ALS) is a neurodegenerative disease that causes damage of upper motor neuron and lower motor neuron. Our objective was to describe the incidence and demographic characteristics of ALS and to analyze its diagnosis and management in Togo. MATERIALS AND METHODS: A retrospective and descriptive study of patient's observations was conducted in the department of neurology of the teaching hospital in Lomé during a 10 years period (2000 to 2009). The diagnosis of ALS was made according to the clinical classification of El Escorial. RESULTS: 5 cases of ALS were diagnosed, representing 0.049% of the hospitalizations in the department of neurology. The average age of patients was 49 years [range: 24 - 67 years] and the average evolution of the disease was 17.6 months [range: 6 - 36 months]. All the patients were men. The treatment was symptomatic in every case. Treatment with Riluzole was not delivered. During the follow-up, one patient died from respiratory complications. CONCLUSION: The El Escorial criteria should be made more accessible for a larger audience, as the availability and classification of care management relies heavily on diagnosed cases, namely early diagnosis.
La sclérose latérale amyotrophique (SLA) est une maladie dégénérative du motoneurone qui atteint les deux neurones de la voie motrice volontaire et qui est cliniquement définie. BUT: Avoir des données préliminaires au Togo concernant la SLA. MATÉRIEL ET MÉTHODES: Il s'agit d'une étude rétrospective et descriptive sur les observations de patients en service de neurologie du CHU campus de Lomé sur une durée de 2000 à 2009. Sont inclus, les patients répondant aux critères de SLA certaine selon les critères d'El Escorial. RÉSULTATS: Cinq (5) cas de SLA ont été diagnostiqués, représentant 0,049% des hospitalisations du service. L'âge moyen était de 49 ans [24; 67 ans] avec une durée moyenne d'évolution de 17,6 mois [6; 36 mois]. Tous les patients étaient de sexe masculin. Le traitement a été symptomatique dans tous les cas. Le traitement au Riluzole n'a pas pu être institué. Un patient était décédé plus tard de complications respiratoires. CONCLUSION: Les critères d'El Escorial doivent être vulgarisés, car la disponibilité et la codification de la prise en charge dépendent du nombre des cas diagnostiqués et surtout de la précocité du diagnostic.
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Cerebral aneurysms secondary to cerebral vasculitis related to systemic lupus erythematosus are rare. We report a 31-year-old woman who presented with a lupus flare associated with inaugural generalized seizures. Computed tomography angiography showed subarachnoid hemorrhage by rupture of a cerebellar artery fusiform aneurysm. Later, despite the initiation of corticosteroids and cyclophosphamide, she presented a second cerebral hemorrhage due to the rupture of a new aneurysm in lenticulostriates arteries. The outcome was fatal. We discuss the frequency and management of this severe complication of systemic lupus erythematosus.