RESUMO
Proliferative vitreoretinal retinopathy (PVR) is a very severe complication of vitreoretinal surgery. PVR is characterised by a complex cellular reaction. This corresponds to a vitreoretinal wound healing reaction and leads to tractional retinal detachment fixed by membranes. A rational goal of treatment is the removal of active cells and membranes, particularly the whole vitreous body; this can only be achieved surgically.
Assuntos
Descolamento Retiniano/diagnóstico , Descolamento Retiniano/cirurgia , Cirurgia Vitreorretiniana/efeitos adversos , Vitreorretinopatia Proliferativa/diagnóstico , Vitreorretinopatia Proliferativa/cirurgia , Diagnóstico Diferencial , Medicina Baseada em Evidências , Humanos , Descolamento Retiniano/etiologia , Resultado do Tratamento , Vitrectomia/métodos , Vitreorretinopatia Proliferativa/etiologia , Corpo Vítreo/cirurgiaRESUMO
In order to analyze the effects of peroxisome proliferator-activated receptor-gamma (PPARgamma) activation on renal cell carcinomas we utilized several cell lines that were treated with the high affinity PPARgamma agonist, troglitazone. Incubation of RCC cells with troglitazone resulted in reduced secretion of growth factors that was due to the inhibition of MAP kinase signaling and reduced nuclear localized expression of relB and HIF1alpha. Interestingly, the cell lines used showed a different sensitivity towards apoptosis induction that did not correlate with the inhibition of growth factors or expression of pro- and antiapoptotic molecules. To overcome this resistance the cells were treated with a combination of troglitazone and the proteasome inhibitor, bortezomib. The combination of both compounds induced apoptosis even in cells resistant to both agents alone, due to increased induction of ER-stress and caspase-3 mediated cell death.
Assuntos
Antineoplásicos/farmacologia , Carcinoma de Células Renais/metabolismo , Cromanos/farmacologia , Resistencia a Medicamentos Antineoplásicos , Neoplasias Renais/metabolismo , PPAR gama/agonistas , Inibidores de Proteassoma , Tiazolidinedionas/farmacologia , Indutores da Angiogênese/metabolismo , Apoptose/efeitos dos fármacos , Ácidos Borônicos/farmacologia , Bortezomib , Caspase 3/metabolismo , Linhagem Celular Tumoral , Citocinas/biossíntese , Sinergismo Farmacológico , Retículo Endoplasmático/fisiologia , Humanos , Subunidade alfa do Fator 1 Induzível por Hipóxia/fisiologia , Peptídeos e Proteínas de Sinalização Intercelular/metabolismo , Proteínas Quinases Ativadas por Mitógeno/fisiologia , NF-kappa B/fisiologia , PPAR gama/antagonistas & inibidores , Complexo de Endopeptidases do Proteassoma/metabolismo , Pirazinas/farmacologia , Transdução de Sinais , TroglitazonaRESUMO
Proliferative vitreoretinopathy (PVR) is the most important complication of retinal detachment and vitreoretinal surgery. PVR is considered to represent a maladapted retinal wound repair process; proliferation of retinal and immune cells induces the formation of epiretinal membranes which cause tractional retinal detachment. This publication gives a brief overview on the pathogenesis and operative treatment of PVR as well as on adjunct pharmacological therapy which may target the components of the proliferative process. At the moment surgical approaches are the first choice for the treatment of PVR. Scleral buckling provides good anatomic results in the treatment of a PVR stage B or C1 / C2. From stage C 3 onwards vitrectomy offers advantage.
Assuntos
Descolamento Retiniano/complicações , Descolamento Retiniano/terapia , Vitrectomia/efeitos adversos , Vitreorretinopatia Proliferativa/etiologia , Vitreorretinopatia Proliferativa/terapia , HumanosRESUMO
Zoledronic acid (ZA) is a nitrogen-containing bisphosphonate with antitumor activity used to treat patients with malignant diseases. ZA treatment induces, as a side effect, inflammatory responses, which are accompanied by expansion of gammadelta T cells. In our study, we analyzed the function and differentiation of monocyte-derived immature and lipopolysaccharide (LPS)-stimulated dendritic cells (moDCs) treated with different ZA concentrations, which are achieved in patients. We found that moDC activation with TLR4 ligand LPS is modulated by ZA. The expression of maturation markers was diminished with increasing ZA levels upon LPS activation. The migratory capacity, interleukin-12 secretion and generation of cytotoxic- T-cell responses were reduced at higher ZA levels. Increasing ZA concentrations downregulated nuclear factor-kappaB family members and interferon-regulatory factor (IRF)-3. Surprisingly, in immature moDCs, low ZA concentrations caused upregulation of RelB, c-Rel, IRF-3 and IRF-8. We conclude that ZA concentrations used to treat patients have inhibitory effects on DC activation. This might lead to immunosuppression or result in infectious complications.
Assuntos
Células Dendríticas/fisiologia , Difosfonatos/farmacologia , Imidazóis/farmacologia , Monócitos/citologia , Receptor 4 Toll-Like/fisiologia , Conservadores da Densidade Óssea/farmacologia , Células Cultivadas , Citocinas/metabolismo , Células Dendríticas/citologia , Células Dendríticas/efeitos dos fármacos , Humanos , Lipopolissacarídeos/farmacologia , Monócitos/efeitos dos fármacos , Linfócitos T Citotóxicos/citologia , Receptor 4 Toll-Like/efeitos dos fármacos , Ácido ZoledrônicoRESUMO
Water accumulation in retinal glial (Müller) and neuronal cells resulting in cellular swelling contributes to the development of retinal edema and neurodegeneration. Intravitreal administration of neurotrophins such as brain-derived neurotrophic factor (BDNF) is known to promote survival of retinal neurons. Here, we show that exogenous BDNF inhibits the osmotic swelling of Müller cell somata induced by superfusion of rat retinal slices or freshly isolated cells with a hypoosmotic solution containing barium ions. BDNF also inhibited the osmotic swelling of bipolar cell somata in retinal slices, but failed to inhibit the osmotic soma swelling of freshly isolated bipolar cells. The inhibitory effect of BDNF on Müller cell swelling was mediated by activation of tropomyosin-related kinase B (TrkB) and transactivation of fibroblast growth factor receptors. Exogenous basic fibroblast growth factor (bFGF) fully inhibited the osmotic swelling of Müller cell somata while it partially inhibited the osmotic swelling of bipolar cell somata. Isolated Müller cells displayed immunoreactivity of truncated TrkB, but not full-length TrkB. Isolated rod bipolar cells displayed immunoreactivities of both TrkB isoforms. Data suggest that the neuroprotective effect of exogenous BDNF in the retina is in part mediated by prevention of the cytotoxic swelling of retinal glial and bipolar cells. While BDNF directly acts on Müller cells by activation of TrkB, BDNF indirectly acts on bipolar cells by inducing glial release of factors like bFGF that inhibit bipolar cell swelling.
Assuntos
Fator Neurotrófico Derivado do Encéfalo/farmacologia , Edema/tratamento farmacológico , Células Ependimogliais/efeitos dos fármacos , Fatores de Crescimento de Fibroblastos/metabolismo , Células Bipolares da Retina/efeitos dos fármacos , Transdução de Sinais/efeitos dos fármacos , Análise de Variância , Animais , Bário/farmacologia , Fator Neurotrófico Derivado do Encéfalo/metabolismo , Edema/etiologia , Feminino , Técnicas In Vitro , Masculino , Pressão Osmótica , Proteína Quinase C/metabolismo , Ratos , Ratos Long-Evans , Receptor trkB/metabolismo , Retina/citologia , Estatísticas não Paramétricas , Fatores de TempoRESUMO
Muller (radial glial) cells span the entire thickness of the retina, and contact and ensheath every type of neuronal cell body and process. This morphological relationship is reflected by a multitude of functional interactions between retinal neurons and Muller cells, including extracellular ion homeostasis and glutamate recycling by Muller cells. Virtually every disease of the retina is associated with a reactive Muller cell gliosis. Muller cell gliosis may either support the survival of retinal neurons or accelerate the progress of neuronal degeneration. Muller cells are key mediators of nerve cell protection, especially via release of basic fibroblast growth factor, via uptake and degradation of the excitotoxin glutamate, and via secretion of the antioxidant glutathione. Neovascularization during hypoxic conditions is mediated by Muller cells via release of vascular endothelial growth factor and transforming growth factor beta or via direct contact to endothelial cells. Primary Muller cell insufficiency has been suggested to be the cause of different cases of retinal degeneration including hepatic and methanol-induced retinopathy and glaucoma. It is conceivable that, in the future, new therapeutic strategies may utilize Muller cells for, e.g., somatic gene therapy or transdifferentiation of retinal neurons from dedifferentiated Muller cells.
Assuntos
Retina/fisiopatologia , Degeneração Retiniana/fisiopatologia , Animais , Humanos , Retina/patologia , Doenças Retinianas/fisiopatologiaRESUMO
Arachidonic acid, which is thought to be involved in pathogenetic mechanisms of the central nervous system, has been shown previously to modulate neuronal ion channels and the glutamate uptake carrier of retinal glial (Müller) cells. We have used various configurations of the patch-clamp technique to determine the effects of arachidonic acid on the K+ currents of freshly isolated Müller glial cells from rabbit and human. Arachidonic acid reduced the peak amplitude of the transient (A-type) outward K+ currents in a dose-dependent and reversible manner, with a 50% reduction achieved by 4.1 microM arachidonic acid. The inward rectifier-mediated currents remained unchanged after arachidonic acid application. The amplitude of the Ca(2+)-activated K+ outward currents (KCa), which were blocked by 1 mM tetraethylammonium chloride and 40 nM iberiotoxin, respectively, was dose-dependently elevated by bath application of arachidonic acid. The activation curve of the KCa currents shifted towards more negative membrane potentials. Furthermore, arachidonic acid was found to suppress inwardly directed Na+ currents. In cell-attached recordings with 3 mM K+ in the bath and 130 mM K+ in the pipette, the KCa channels of rabbit Müller cells displayed a linear current-voltage relation, with a mean slope conductance of 102 pS. In excised patches, the slope conductance was 220 pS (150 mM K+i/130 mM K+o). The opening probability of the KCa channels increased during membrane depolarization and during elevation of the free Ca2+ concentration at the intracellular face of the membrane patches. Bath application of arachidonic acid caused a reversible increase of the single-channel opening probability, as well as an increase of the number of open channels. Arachidonic acid did not affect the single-channel conductance. Since arachidonic acid also stimulates the KCa channel activity in excised patches, the action of arachidonic acid is assumed to be independent of changes of the intracellular calcium concentration. Our results demonstrate that arachidonic acid exerts specific effects on distinct types of K+ channels in retinal glial, cells. In pathological cases, elevated arachidonic acid levels may contribute to prolonged Müller cell depolarizations, and to the initiation of reactive glial cell proliferation.
Assuntos
Ácido Araquidônico/farmacologia , Neuroglia/metabolismo , Canais de Potássio/metabolismo , Retina/metabolismo , Animais , Cálcio/fisiologia , Estimulação Elétrica , Eletrofisiologia , Proteínas de Ligação ao GTP/metabolismo , Humanos , Potenciais da Membrana/efeitos dos fármacos , Potenciais da Membrana/fisiologia , Neuroglia/efeitos dos fármacos , Técnicas de Patch-Clamp , Canais de Potássio/efeitos dos fármacos , Proteína Quinase C/metabolismo , Coelhos , Retina/citologia , Retina/efeitos dos fármacos , SoluçõesRESUMO
There is strong evidence for the presence of nucleotide (P2) receptors in sensory neurons, which might play a role in the transmission of pain signals. In contrast, virtually nothing is known about P2 receptors in satellite glial cells (SGCs), which are the main glial cells in sensory ganglia. We investigated the possibility that P2 receptors exist in SGCs in murine trigeminal ganglia, using Ca(2+) imaging, patch-clamp recordings, and immunohistochemistry. We found that ATP caused an increase in intracellular Ca(2+) concentration ([Ca(2+)](i)) in SGCs. As adenosine had no effect on [Ca(2+)](i), and the P2 receptor antagonist pyridoxalphosphate-6-azophenyl-2',4'-disulfonic acid largely blocked the response to ATP we conclude that P1 receptors did not contribute to the responses. We obtained the following evidence that the responses to ATP were mediated by metabotropic P2Y receptors: (i) persistence of the responses in Ca(2+)-free solution, (ii) inhibition of the response by cyclopiazonic acid, (iii) [Ca(2+)](i) increases in response to the P2Y agonists uridine triphosphate, adenosine thiodiphosphate, and 2-methylthio ADP, and (iv) failure of the P2X agonist alpha,beta-methylene ATP to elicit a response. Agonists of P2Y(1) receptors and uridine triphosphate, an agonist at P2Y(2) and P2Y(4) receptors, induced [Ca(2+)](i) increases suggesting that at least these P2Y receptor subtypes are present on SGCs. Using an antibody against the P2Y(4) receptor, we found immunopositive SGCs. Patch-clamp recordings of SGCs did not reveal any inward current due to ATP. Therefore, there was no evidence for the activation of ionotropic P2X receptors under the present conditions. The results indicate the presence of functional nucleotide (P2Y) receptors in SGCs.
Assuntos
Fura-2/análogos & derivados , Neuroglia/fisiologia , Fosfato de Piridoxal/análogos & derivados , Receptores Purinérgicos P2/fisiologia , Gânglio Trigeminal/fisiologia , Trifosfato de Adenosina/administração & dosagem , Trifosfato de Adenosina/agonistas , Animais , Cálcio/metabolismo , Carbocianinas/metabolismo , Citofotometria/instrumentação , Citofotometria/métodos , Relação Dose-Resposta a Droga , Interações Medicamentosas , Inibidores Enzimáticos/administração & dosagem , Feminino , Fura-2/metabolismo , Glutamato-Amônia Ligase/metabolismo , Imuno-Histoquímica , Técnicas In Vitro , Indóis/administração & dosagem , Masculino , Potenciais da Membrana/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos BALB C , Técnicas de Patch-Clamp/instrumentação , Técnicas de Patch-Clamp/métodos , Inibidores da Agregação Plaquetária/administração & dosagem , Fosfato de Piridoxal/administração & dosagemRESUMO
PURPOSE: Müller glial cells from the human retina express purinergic P2X(7) receptors. Because extracellular adenosine triphosphate (ATP) is assumed to be a mediator of the induction or maintenance of gliosis, this study was undertaken to determine whether the expression of these receptors is different in human Müller cells obtained from retinas of healthy donors and of patients with choroidal melanoma and proliferative vitreoretinopathy (PVR). METHODS: Human Müller cells were enzymatically isolated from donor retinas, and whole-cell patch-clamp recordings were made to characterize the density of the P2X(7) currents and the activation of currents through Ca2+-activated K+ channels of big conductance (I:(BK)) that reflects the increase of the intracellular Ca2+ concentration. RESULTS: Stimulation by external ATP or by benzoylbenzoyl ATP (BzATP) evoked both release of Ca2+ from thapsigargin-sensitive intracellular stores and opening of Ca2+ -permeable P2X(7) channels. These responses caused transient and sustained increases in I:(BK). In Müller cells from patients with PVR, the mean density of the BzATP-evoked cation currents was significantly greater compared with cells from healthy donors. As a consequence, such cells displayed an enlarged I:(BK) during application of purinergic agonists. ATP and BzATP increased the DNA synthesis rate of cultured cells. This effect could be reversed by blocking the I:(BK). CONCLUSIONS: The increased density of P2X(7) receptor channels may permit a higher level of entry of extracellular Ca2+ into cells from patients with PVR. Enhanced Ca2+ entry and the subsequent stronger activation of I:(BK) may contribute to the induction or maintenance of proliferative activity in gliotic Müller cells during PVR.
Assuntos
Trifosfato de Adenosina/análogos & derivados , Gliose/metabolismo , Neuroglia/metabolismo , Receptores Purinérgicos P2/metabolismo , Retina/metabolismo , Vitreorretinopatia Proliferativa/metabolismo , Trifosfato de Adenosina/farmacologia , Técnicas de Cultura de Células , Neoplasias da Coroide/metabolismo , DNA/biossíntese , Replicação do DNA , Eletrofisiologia , Humanos , Melanoma/metabolismo , Potenciais da Membrana/fisiologia , Neuroglia/efeitos dos fármacos , Técnicas de Patch-Clamp , Receptores Purinérgicos P2X7 , Retina/efeitos dos fármacos , Regulação para CimaRESUMO
PURPOSE: To determine the electrophysiological properties of Müller (glial) cells from experimentally detached rabbit retinas. METHODS: A stable local retinal detachment was induced by subretinal injection of a sodium hyaluronate solution. Müller cells were acutely dissociated and studied by the whole-cell voltage-clamp technique. RESULTS: The cell membranes of Müller cells from normal retinas were dominated by a large inwardly rectifying potassium ion (K+) conductance that caused a low-input resistance (<100 M(Omega)) and a high resting membrane potential (-82 +/- 6 mV). During the first week after detachment, the Müller cells became reactive as shown by glial fibrillary acidic protein (GFAP) immunoreactivity, and their inward currents were markedly reduced, accompanied by an increased input resistance (>200 M(Omega)). After 3 weeks of detachment, the input resistance increased further (>300 M(Omega)), and some cells displayed significantly depolarized membrane potentials (mean -69 +/- 18 mV). When PVR developed (in 20% of the cases) the inward K+ currents were virtually completely eliminated. The input resistance increased dramatically (>1000 MOmega), and almost all cells displayed strongly depolarized membrane potentials (-44 +/- 16 mV). CONCLUSIONS: Reactive Müller cells are characterized by a severe reduction of their K+ inward conductance, accompanied by depolarized membrane potentials. These changes must impair physiological glial functions, such as neurotransmitter recycling and K+ ion clearance. Furthermore, the open probability of certain types of voltage-dependent ion channels (e.g., Ca2+-dependent K+ maxi channels) increases that may be a precondition for Müller cell proliferation, particularly in PVR when a dramatic downregulation of both inward current density and resting membrane potential occurs.
Assuntos
Neuroglia/fisiologia , Descolamento Retiniano/fisiopatologia , Animais , Membrana Celular/fisiologia , Eletrofisiologia , Feminino , Técnica Indireta de Fluorescência para Anticorpo , Proteína Glial Fibrilar Ácida/metabolismo , Hialuronoglucosaminidase/toxicidade , Masculino , Potenciais da Membrana/fisiologia , Técnicas de Patch-Clamp , Potássio/metabolismo , Coelhos , Retina/efeitos dos fármacos , Descolamento Retiniano/induzido quimicamente , Vitreorretinopatia Proliferativa/induzido quimicamente , Vitreorretinopatia Proliferativa/fisiopatologiaRESUMO
PURPOSE: To determine whether the expression of voltage-gated Ca2+ channels in human Müller glial cells changes during normal aging and in cells from patients with proliferative vitreoretinopathy (PVR). METHODS: Müller cells were enzymatically isolated from retinas of healthy donors and from excised retinal pieces of patients with PVR, and the whole-cell, voltage-clamp technique was used to characterize the current densities of transient, low-voltage-activated calcium channels and of sustained. high-voltage-activated calcium channels, respectively. To obtain maximal currents through both channel types, Na+ ions were used as the charge carrier. RESULTS: During normal aging, Müller cells developed a hypertrophy, as indicated by an increase of the cell membrane capacitance. The mean membrane capacitance of cells from aged donors (> or = 60 years old) was elevated by 25% compared with cells from younger donors. The hypertrophy was not accompanied by a changed density of low-voltage-activated currents, whereas the density of the high-voltage-activated currents was enhanced by 76%. The density of the high-voltage-activated currents increased in correlation with the increase of the cell membrane capacitance and with the age of the donors. In the case of PVR, Müller cells displayed a strong hypertrophy accompanied by a downregulation of both current types by approximately 65%. CONCLUSIONS: Both normal aging and PVR cause a gliotic reactivity of human Müller cells, as indicated by their hypertrophy. The type of reactivity, however, differs between the two conditions. Normal aging is accompanied by an increased expression of voltage-gated Ca2+ channels, whereas in PVR Ca2+ channel expression is decreased.
Assuntos
Envelhecimento/fisiologia , Canais de Cálcio/metabolismo , Neuroglia/metabolismo , Retina/metabolismo , Vitreorretinopatia Proliferativa/metabolismo , Adulto , Idoso , Idoso de 80 Anos ou mais , Cálcio/metabolismo , Eletrofisiologia , Humanos , Hipertrofia , Potenciais da Membrana/fisiologia , Pessoa de Meia-Idade , Neuroglia/patologia , Retina/patologia , Sódio/metabolismo , Vitreorretinopatia Proliferativa/patologiaRESUMO
PURPOSE: To determine the involvement of Ca(2+)-activated K(+) channels of big conductance (BK) and of Ca(2+) channels in the regulation of DNA synthesis in cultured guinea pig Müller cells. DNA synthesis was stimulated by elevated extracellular potassium, by serum, or by epidermal growth factor. METHODS: Dissociated retinas from guinea pigs were cultured for 8 days. Just before confluence was achieved, the cultures were treated with the test substances in serum-free or serum-containing media. The rates of DNA synthesis were assessed by a quantitative bromodeoxyuridine immunoassay. The intracellular Ca(2+) concentration was measured by the fura-2 fluorescence technique. RESULTS: Blocking the BK channels with tetraethylammonium or by iberiotoxin had no effect at normal extracellular K(+) (5.8 mM) but decreased the rate of DNA synthesis at higher extracellular K(+) (10 or 25 mM). Epidermal growth factor-induced DNA synthesis was decreased by block of BK channels or by application of the Ca(2+) channel blockers nimodipine and flunarizine. Application of epidermal growth factor elevated the intracellular Ca(2+) concentration of cultured Müller cells. This elevation was diminished by co-application of iberiotoxin or of flunarizine. CONCLUSIONS: The activity of BK channels is necessary for elevated DNA synthesis in Müller cells when their membranes are depolarized and/or when the Ca(2+) influx into Müller cells is increased by growth factors. BK channels may contribute to the maintenance of DNA synthesis by increasing mitogen-induced increase in intracellular Ca(2+) concentration.
Assuntos
DNA/biossíntese , Neuroglia/metabolismo , Canais de Potássio Cálcio-Ativados , Canais de Potássio/metabolismo , Animais , Sangue , Cálcio/metabolismo , Cálcio/farmacologia , Bloqueadores dos Canais de Cálcio/farmacologia , Canais de Cálcio/efeitos dos fármacos , Canais de Cálcio/metabolismo , Divisão Celular/efeitos dos fármacos , Células Cultivadas , Replicação do DNA/efeitos dos fármacos , Eletrofisiologia , Fator de Crescimento Epidérmico/farmacologia , Fura-2/metabolismo , Cobaias , Canais de Potássio Ativados por Cálcio de Condutância Alta , Potenciais da Membrana , Neuroglia/citologia , Peptídeos/farmacologia , Potássio/farmacologia , Bloqueadores dos Canais de Potássio , Tetraetilamônio/farmacologiaRESUMO
PURPOSE: To determine differences of K+ channel activity between Müller glial cells obtained from retinas of healthy human donors and of patients with retinal detachment and proliferative vitreoretinopathy. METHODS: Müller cells were enzymatically isolated from retinas of healthy donors and from excised retinal pieces of patients. The whole-cell and the cell-attached configurations of the patch-clamp technique were used to characterize the current densities of different K+ channel types and the activity of single Ca2+ -activated K+ channels of big conductance (BK). RESULTS: Cells from patients displayed a less negative mean membrane potential (-52.8 mV) than cells from healthy donors (-80.6 mV). However, the membrane potentials in cells from patients scattered largely between -6 and -99 mV. The inwardly rectifying K+ permeability in cells from patients was strongly reduced (0.3 pA/pF) when compared with cells from healthy donors (6.0 pA/pF). At the resting membrane potential, single BK channels displayed a higher mean activity (open probability, Po, and channel current amplitude) in cells from patients (Po, 0.30) than in cells from healthy donors (Po: 0.03). The variations of BK current amplitudes were correlated with the variations of the membrane potential. CONCLUSIONS: The dominant expression of inwardly rectifying channels in cells from healthy donors is thought to support important glial cell functions such as the spatial buffering of extracellular K+. The downregulation of these channels and the less negative mean membrane potential in cells from patients should impair spatial buffering currents and neurotransmitter clearance. The increased activity of BK channels may support the proliferative activity of gliotic cells via feedback regulation of Ca2+ entry and membrane potential.
Assuntos
Neuroglia/metabolismo , Canais de Potássio/metabolismo , Retina/metabolismo , Vitreorretinopatia Proliferativa/metabolismo , 4-Aminopiridina/farmacologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Cálcio/farmacologia , Eletrofisiologia , Feminino , Humanos , Masculino , Potenciais da Membrana , Pessoa de Meia-Idade , Neuroglia/citologia , Técnicas de Patch-Clamp , Permeabilidade , Potássio/metabolismo , Canais de Potássio/efeitos dos fármacos , Retina/citologiaRESUMO
Whereas in the brain, the activity of the neurons is supported by several types of glial cells such as astrocytes, oligodendrocytes, and ependymal cells, the retina (evolving from the brain during ontogenesis) contains only one type of macroglial cell, the Müller (radial glial) cells, in most vertebrates including the anurans. These cells span the entire thickness of the tissue, and thereby contact and ensheath virtually every type of neuronal cell body and process. This intimate topographical relationship is reflected by a multitude of functional interactions between retinal neurons and Müller glial cells. Müller cells are the principal stores of retinal glycogen, and are thought to fuel retinal neurons with substrate (lactate/pyruvate) for their oxidative metabolism. Furthermore, Müller cells are involved in the control and homeostasis of many constituents of the extracellular space, such as potassium and perhaps other ions, signaling molecules, and of the extracellular pH. They also seem to play important roles in recycling mechanisms of photopigment molecules and neurotransmitter molecules such as glutamate and GABA. By containing the main retinal stores of glutathione, Müller cells may protect retinal neurons against free radicals. Moreover, Müller cells express receptors for many neuroactive substances, and may also release such substances to their neighbouring neurons. Thus, Müller cells exert many functions crucial for signal processing in the normal retina. Moreover, Müller cells change their properties in cases of retinal disease and injury, and may either support the survival of neuronal cells or accelerate the progress of neuronal degeneration.
Assuntos
Anuros/anatomia & histologia , Neuroglia , Retina , Animais , Anuros/metabolismo , Barreira Hematorretiniana , Canais de Cálcio/metabolismo , Metabolismo Energético , Radicais Livres/metabolismo , Homeostase , Concentração de Íons de Hidrogênio , Neuroglia/citologia , Neuroglia/metabolismo , Neuroglia/patologia , Neurotransmissores/metabolismo , Canais de Potássio/metabolismo , Retina/citologia , Retina/metabolismo , Retina/patologia , Ácido gama-Aminobutírico/metabolismoRESUMO
Previous investigations suggest a crucial role of the pedunculopontine tegmental nucleus (PPTg) in elicitation of cortical EEG alterations during paradoxical sleep. In order to reveal a participation of this nucleus in the regulation of the cortical EEG also during waking states and slow wave sleep unilateral ibotenic acid lesions of this nucleus were made in 12 Long-Evans rats. The lesion induced alterations of the frontal and occipital EEG were determined by FFT spectral power analysis. The lesion caused ipsilaterally a suppression of the hippocampal theta rhythm recorded on the occipital cortex during exploratory sniffing. During head washing the frontal delta waves were found to be elevated ipsilaterally. Suppressions of the slow sleep waves were found in the contralateral occipital as well as in the frontal EEG. The results indicate involvements of the PPTg in the regulation of the hippocampal and of the motor activity in waking states as well as in the regulation of the sleep delta waves.
Assuntos
Ponte/fisiologia , Tegmento Mesencefálico/fisiologia , Animais , Contagem de Células/efeitos dos fármacos , Eletroencefalografia , Ácido Ibotênico/farmacologia , Masculino , Atividade Motora/fisiologia , NADPH Desidrogenase/metabolismo , RatosRESUMO
The flavoid phloretin was used to study large-conductance, Ca2+-activated K+ (BKCa) channel-mediated currents in isolated porcine Müller (retinal glial) cells. In excised membrane patches, application of phloretin (100 microM) increased the open probability of single BKCa channels. In whole-cell records, phloretin increased the amplitude of an outward current which was blockable by iberiotoxin (40 nM) and TEA (1 mM), and, thus, was identified as a BKCa current. Only 50% of the investigated cells expressed phloretin-sensitive BKCa currents. Cells with phloretin-sensitive BKCa currents responded to glutamate (500 microM) with an increase of the amplitude of this current, whereas glutamate had no effects on the K+ currents of cells without BKCa currents. As glutamate is a major neurotransmitter of the retina, and the membrane properties of Müller cells are largely determined by their K+ channels, the observed data may shed new light onto the functions of retinal glia.
Assuntos
Canais de Cálcio/efeitos dos fármacos , Neuroglia/efeitos dos fármacos , Floretina/farmacologia , Canais de Potássio/efeitos dos fármacos , Animais , Ácido Glutâmico/farmacologia , Técnicas de Patch-Clamp , Peptídeos/farmacologia , Suínos , Toxinas Biológicas/farmacologiaRESUMO
The modulation of the activity of Ca2+ -activated K+ (BK) channels by activators of protein kinases A and C, respectively, was studied in cell-attached patches on isolated Müller (retinal glial) cells from rabbits. The BK channel activity was stimulated by membrane depolarization and by increasing of the intracellular Ca2+ concentration. Extracellular exposure to dibutyryl-cAMP, known to stimulate the protein kinase A, increased the open probability of the channels. Exposure to a phorbol ester, as an activator of protein kinase C, strongly reduced the channel activity whereas exposure to the protein kinase inhibitor, staurosporine, stimulated the channel activity. As glial BK channels are modulated in an opposite manner by protein kinases A and C, they may act as a cellular focus of integration of the inputs from different signaling pathways.
Assuntos
Bucladesina/farmacologia , Proteínas Quinases Dependentes de AMP Cíclico/metabolismo , Neuroglia/fisiologia , Canais de Potássio Cálcio-Ativados , Canais de Potássio/fisiologia , Proteína Quinase C/metabolismo , Acetato de Tetradecanoilforbol/farmacologia , Animais , Calcimicina/farmacologia , Cálcio/farmacologia , Proteínas Quinases Dependentes de AMP Cíclico/antagonistas & inibidores , Ativação Enzimática , Inibidores Enzimáticos/farmacologia , Técnicas In Vitro , Ativação do Canal Iônico/fisiologia , Canais de Potássio Ativados por Cálcio de Condutância Alta , Potenciais da Membrana/efeitos dos fármacos , Técnicas de Patch-Clamp , Potássio/farmacologia , Canais de Potássio/efeitos dos fármacos , Proteína Quinase C/antagonistas & inibidores , Coelhos , Retina/citologia , Retina/fisiologia , Transdução de Sinais , Estaurosporina/farmacologiaRESUMO
We show by immunocytochemistry in frog retina that most members of the Kir subfamily are expressed in specific neuronal compartments. However, Kir 6.1, the pore-forming subunit of K(ATP) channels, is expressed exclusively in glial Müller cells. Müller cell endfeet display strong Kir 6.1 immunolabel throughout the retina, whereas the somata are labeled only in the retinal periphery. This spatial pattern is similar to that of Kir 4.1, of the ratio of inward to outward K+ currents, and of spermine/spermidine immunoreactivity. We suggest that the co-expression of Kir 4.1 and Kir 6.1 subunits may enable the cells to maintain their high K+ conductance and hyperpolarized membrane potentials both at high ATP levels (Kir 4.1) and during ATP deficiency (Kir 6.1).
Assuntos
Potenciais da Membrana/fisiologia , Neuroglia/metabolismo , Canais de Potássio Corretores do Fluxo de Internalização , Canais de Potássio/metabolismo , Rana pipiens/metabolismo , Retina/metabolismo , Visão Ocular/fisiologia , Animais , Especificidade de Anticorpos , Canais de Potássio Corretores do Fluxo de Internalização Acoplados a Proteínas G , Imuno-Histoquímica , Neuroglia/citologia , Potássio/metabolismo , Rana pipiens/anatomia & histologia , Retina/citologiaRESUMO
Previous lectin-histochemical and immunocytochemical investigations using fixed tissue revealed perineuronal nets as lattice-like accumulations of extracellular matrix proteoglycans at the surface of several types of neurons. In the present study, perineuronal nets in the rat brain were labelled for the first time in vivo by stereotaxic injections of biotinylated Wisteria floribunda agglutinin (Bio-WFA), as well as in vitro, by incubation of unfixed brain slices with the same lectin. Six days after Bio-WFA injections into the parietal cortex, medial septum, reticular thalamic nucleus and red nucleus, the lectin remaining bound to perineuronal nets was detected by streptavidin/biotinylated peroxidase complexes or red fluorescent Cy3-streptavidin, respectively. Double-fluorescence labelling showed that Bio-WFA applied in vivo reacted with the chondroitin sulphate proteoglycan immunoreactive perineuronal nets in the injection zone. Labelling of perineuronal nets in unfixed slices was obtained with either Cy3-tagged WFA or Bio-WFA and subsequent visualization by Cy3-streptavidin which confirmed the region-dependent distribution patterns and the structural characteristics of perineuronal nets known from histochemical studies. These results provide support for the role of extracellular matrix proteoglycans to maintain a considerable chemical and, probably, spatial heterogeneity of the extracellular space in vivo. The ability of in vivo and in vitro labelling may promote the functional characterization of the extracellular matrix in various brain structures including its species-dependent neuronal association patterns.
Assuntos
Encéfalo/ultraestrutura , Rede Nervosa/metabolismo , Neurônios/metabolismo , Lectinas de Plantas , Animais , Biotina , Química Encefálica/fisiologia , Sulfatos de Condroitina/metabolismo , Matriz Extracelular/efeitos dos fármacos , Matriz Extracelular/metabolismo , Matriz Extracelular/ultraestrutura , Imuno-Histoquímica , Lectinas , Masculino , Rede Nervosa/ultraestrutura , Neurônios/ultraestrutura , Proteoglicanas/metabolismo , Ratos , Receptores de N-AcetilglucosaminaRESUMO
In previous experiments it was found that physostigmine application in unrestrained rats delayed the peak latency of the visual evoked potential (VEP). The present study was carried out to find the putative site that cholinergically mediates the latency delay of rat's VEP. After unilateral ibotenic acid lesions of the nucleus basalis magnocellularis (NBM) and the pedunculopontine tegmental nucleus (PPTg), respectively, the VEPs of freely moving rats were recorded in different behavioural states. While NBM lesion did not alter the behavioural modulation of the VEP latency, the PPTg lesion produced shorter VEP latencies in the occipital cortex of the lesioned hemisphere in high and moderate arousal states. The peak latency shortening was significant for exploratory sniffing. Physostigmine but not nicotine application abolished the shorter VEP latency. The results indicate a muscarinic mechanism within the pontomesencephalic tegmentum that ipsilaterally delayed the VEP latency in high and moderate but not in low arousal states.