[Evaluation of the clinical efficacy of the effect of herbal medicine on the symptoms of SARS-COV-2 associated pharyngitis and the formation of post-covid syndrome]. / Otsenka klinicheskoi effektivnosti vliyaniya original'nogo rastitel'nogo lekarstvennogo preparata na simptomy SARS-CoV-2-assotsiirovannogo tonzillofaringita i formirovanie postkovidnogo sindroma.

Pharyngitis is one of the manifestations of SARS-CoV-2 infection, which features specific changes in the pharyngoscopic pattern, long fluctuating course and increase in symptoms severity after a physical exercise, which requires long-term therapy with topical agents. A comparative analysis of Tonsilgon N effect on the course of SARS-CoV-2 associated pharyngitis as well as post-COVID syndrome development was carried out in this study. 164 patients with acute pharyngitis associated with SARS-CoV-2 were included in the study. Main group (n=81) received Tonsilgon N oral drops in addition to the standard treatment regimens for pharyngitis and control group (n=83) received standard regimen alone. Treatment regimen lasted for 21 days for both groups, followed by follow up examination in 12 weeks for assessment of post-COVID syndrome development. Patients taking Tonsilgon N showed a statistically significant symptoms improvement in throat pain relief (p=0.002) and throat discomfort (p=0.004), however, no statistically significant difference in two groups were observed in inflammation severity based on pharyngoscopy examination (p=0.558). Addition of Tolzilgon N to the regimen reduced occurrence of secondary bacterial infections and therefore use of antibiotics more than 2.8 times (p<0.001). Compared with the control group long term topical therapy with Tolzilgon N showed no increase in occurrence of side effects such as allergic reactions (p=0.311) as well as subjective burning sensations in the throat (p=0.849). In main group occurrence of post-COVID syndrome was observed 3.3 times less than in the control group (7.2% vs 25.9%, p=0.001). These results give us the ground for indication of Tonsilgon N use in the treatment of viral pharyngitis associated with SARS-CoV 2 infection and in prevention of post-COVID syndrome.

[The optimization in the rehabilitation of anosmia after SARS-CoV-2 infection]. / Optimizatsiya sposobov vosstanovleniya obonyaniya pri anosmii, indutsirovannoi SARS-CoV-2-infektsiei.

During SARS-CoV-2 pandemic number of patients with olfactory disorders caused by new coronavirus infection substantially increased, making their rehabilitation an issue of the day. We analyzed influence of the drug Sinupret on the restoration dynamic of the olfactory dysfunction associated with SARS-CoV-2 infection. 187 patients with impaired sense of smell associated with SARS-CoV-2 infection were included in this study: test group consisted of 104 cases in which olfactory training methods were combined with the prescription of the drug Sinupret. Control group consisted of 84 patients those undergone olfactory training alone. During study period in 26 patients restoration of the olfactory disorders have not been achieved, due to the virus induced neurodegenerative nature of the lesion. It was observed that in test group odor detection threshold restoration was on average 3.6 days faster than in control group. At the same time, the use of Sinupret was associated with a statistically significant 29.4% decrease of antibiotic prescription frequency for bacterial complications of SARS-CoV-2. Therefore, we assume that the positive effect of Sinupret on SARS-CoV-2 associated anosmia is caused by its antiviral, secretolytic, anti-inflammatory, decongestant and moderately antibacterial effects in the olfactory epithelium.

Comparative Morphological Characteristics of the Results of Implantation of Decellularized and Recellularized Porcine Skin Scaffolds.

The tissue reaction of pig skin to implantation of decellularized and recellularized dermal matrices on a formed wound defect was evaluated by histological methods on days 2, 5, 8, 16, and 20 after surgery. Differences in tissue response to different matrices were identified. In experimental wounds coated with decellularized dermal matrices, we observed the formation of a scar tissue, which required autodermoplasty on day 12 of the experiment. In wounds coated with recellularized dermal matrices, all layers of the skin completely recovered by day 20 after surgery with the formation of full dermal and epidermal layers. Our findings suggest that reparative morphological changes in the wound depend on the presence of fibroblasts in the implanted dermal matrix.

PECULIARITIES OF EVALUATION OF THE ORAL FLUID ANTIOXIDANT ACTIVITY IN PATIENTS WITH LOCAL OR SYSTEMIC DISEASES.

The aim - in the given research, the difficulties in interpreting the study results of oxidative homeostasis of oral fluid are analyzed. Changes in the total antioxidant activity of blood and saliva can be multidirectional - an increase or decrease in the oral fluid indicator and a reduction in the parameter of blood plasma can be recorded. To resolve the emerging difficulties, there was proposed a parallel assessment of the dynamics of changes in the total antioxidant activity of blood plasma and oral fluid in the patients of 4 groups with nosological forms of fundamentally different in the distribution and localization of the pathological process, which include: phlegmons of the maxillofacial region, partial absence of teeth, type 2 diabetes mellitus and the pelvic inflammatory diseases. As a result of the conducted studies, it was shown that a simultaneous decrease in the total antioxidant activity of blood plasma and oral fluid was attributable to the chronic long-term somatic diseases of a systemic character with a significant metabolic disorder, such as type 2 diabetes mellitus. A decrease in the total antioxidant activity of blood plasma and the unchanged oral fluid index was characteristic of somatic diseases of limited prevalence without affection of the maxillofacial region's tissues. In our case, such an example was a chronic inflammatory disease of the uterus with a combined course of bilateral salpingoophoritis. An increase in the oral fluid's total antioxidant activity on the background of a normal or even slightly reduced level of the antioxidant potential of blood plasma was characteristic of dental diseases. The latter situation was most likely for the dental profile diseases, in which damage to the oral tissues can provoke the leaching of cellular contents, including endogenous antioxidants or other components of regenerative activity in the oral fluid. Herein, changes in the antioxidant activity of blood plasma may reflect the prevalence of a pathological process at the systemic level or its limitation only to the dentoalveolar system's tissues and elements. As an example of such a situation, the patients with phlegmon of the maxillofacial region or patients with partial absence of teeth can be cited.

Inactivation of Epigenetic Regulators due to Mutations in Solid Tumors.

Main factors involved in carcinogenesis are associated with somatic mutations in oncogenes and tumor suppressor genes representing changes in the DNA nucleotide sequence. Epigenetic changes, such as aberrant DNA methylation, modifications of histone proteins, and chromatin remodeling, are equally important in the development of human neoplasms. From this perspective, mutations in the genes encoding key participants of epigenetic regulation are of particular interest including enzymes that methylate/demethylate DNA, enzymes that covalently attach or remove regulatory signals from histones, components of nucleosome remodeling multiprotein complexes, auxiliary proteins and cofactors of the above-mentioned molecules. This review describes both germline and somatic mutations in the key epigenetic regulators with emphasis on the latter ones in the solid human tumors, as well as considers functional consequences of these mutations on the cellular level. In addition, clinical associations of the somatic mutations in epigenetic regulators are presented, as well as DNA diagnostics of hereditary cancer syndromes due to germline mutations in the SMARC proteins and chemotherapy drugs directly affecting the altered epigenetic mechanisms for treatment of patients with solid neoplasms. The review is intended for a wide range of molecular biologists, geneticists, oncologists, and associated specialists.

Estimation of Decellularization and Recellularization Quality of Tissue-Engineered Constructions by the Chemiluminescence Method.

It was found that the chemiluminescence intensity in native and recellularized tissues of rat muscular organs as well as in their decellularized scaffolds can serve as an express criterion that, along with ultrastructural analysis, makes it possible to perform quantitative assessment of the viability of cellular structures in biological samples of the diaphragm.

[Radiation-associated changes in salivation of patients with cancer of maxillofacial region]. / Radioindutsirovannye izmeneniia sliunootdeleniia u patsientov so zlokachestvennymi novoobrazovaniiami cheliustno-litsevoi oblasti.

The radiation has an antitumor effect and causes radiation reactions and damage to surrounding tissues within the framework of combined antitumor treatment of patients with cancer of maxillofacial region. It also has an irreversible effect on the production of saliva by large and small salivary glands, and this must be taken into account when planning radiation therapy for this group of patients.

Dynamics of the Pro-Oxidant/Antioxidant System Parameters in Wound Discharge and Plasma in Experimental Purulent Wound during Its Technological Liquid Phase Treatment.

The dynamics of the pro-oxidant/antioxidant system of rabbits was studied at the local (in exudate) and systemic (blood) levels during therapy of purulent wounds in liquid medium with the use of a programmed device and the efficiency of the proposed technological method was compared with that of classical methods for the treatment of these wounds. More rapid recovery of the indicators of the pro-oxidant/antioxidant system to physiological values in the postoperative period was observed after treatment by the proposed method (7 days vs. 10 days after standard treatment), these results attest to strengthening of the adaptation potential in laboratory animals. The intensity of free radical oxidation in the exudate decreased by 26.6% in animals treated by the technological method. These data indicated acceleration of the regenerative processes at the local level.

[The metabolic correction of the experimental alloxan diabetes by means of the antioxidant remedies].

The research concerning the effect of the remedies with the different antioxidant mechanisms on the indices of carbohydrate and lipid metabolism as well as the thiol link of the antioxidant system and the functional detoxication system in modeling conditions of the decompensated alloxan diabetes mellitus have been presented. The research was performed on 105 white nonlinear male-rats with initial body weight (b.w.) 200-230 g, divided into seven groups. Group 1 has consisted of the intact animals. Group 2 (the comparison group) has consisted of the animals suffering from the severe alloxan diabetes mellitus (intraperitoneal administration of alloxan monohydrate at 10 mg/100 g b.w. three times, with an interval of one day, against fasting) without correction. In groups 3-7 diabetes mellitus has also been modeled. The animals of group 3 were fed by the diet with the supplement containing the antioxidant complex with high content of carotenoid substances (1 mg lutein per day, orally for a month before simulations of diabetes mellitus and for a month after). The animals of group 4 have been given the sodium dichloroacetate (15 mg/100g bw) with drinking water for the month before and a month after the modeling of diabetes. The animals of group 5 have been injected with the reduced glutathione (15 mg/100 g of b.w.) daily one week prior to administration of alloxan and until the end of the experiment, the animals of group 6 have been injected with the quercithin (10 mg/100 g of b.w.) and the rats of group 7 have been given the water with the reduced content of deuterium (91 mg/l) instead of the usual drinking water. The development of experimental alloxan diabetes mellitus in rats has been accompanied by the increase in blood plasma of glucose concentration by 6.9-fold, cholesterol and lipoproteins of low density by 66 and 100% respectively. The compensatory activity increase of the antiradical protective enzymes (glutathione peroxidase and glutathione reductase) as well as the concentration decrease of reduced glutathione in erythrocytes by 11% have been revealed. According to the data concerning the level of the molecules with medium and low mass in blood plasma as well as in erythrocytes, the evident endogenous intoxication has taken place. The performed researches have proved the presence of the significant hypoglycemic effect of the usage of sodium dichloroacetate, of the water with the reduced content of deuterium and of quercithin - the glucose concentration in these groups was lower than in the comparison group by 36, 33 and 47% respectively. In the same groups as well as in the group of rats injected with glutathione the hypolipidemic effect of various manifestation degree has been detected, the most evident in animals from group 7 in which all the indices except triglycerides haven't differed from indices of the control group. The usage of various corrective methods has also assisted the adequate functioning of the antioxidant system that has been proved by the normalization of glutathione content except of groups 3 and 6 in which its concentration was lower than the control indices by 25 and 12% respectively. Endogenous intoxication has been significantly lower in groups 4, 5 and 7 in which the level of molecular of medium and low mass in blood plasma was normal and only in erythrocyte mass was increased by 49-74% than in the control group. Thus, the data obtained prove the usage availability of the antioxidant remedies in the complex therapy in patients suffering from the diabetes mellitus. The most interesting is the usage of antioxidants with the indirect mechanism of action, i.e. the water with the reduced content of deuterium and sodium dichloroacetate. Quercithin and the reduced glutathione deserve attention as well, and considering the hypoglycemic effect of the first one and the metabolic effects of glutathione aimed at the functioning maintenance of the non-specific organism resistance, the possibility of the combined usage of both antioxidants should be taken into account.

[SELECTION OF MINI-INVASIVE PALLIATIVE DECOMPRESSION METHOD IN PATIENTS WITH MALIGNANT OBSTRUCTION OF BILIARY DUCTS BASED ON THE STUDY OF BIOCHEMICAL BILE INDEXES].

The article gives our own experience in the determination and study of additional laboratory criteria (total lipids, cholesterol, maximum and area chemiluminescence, antioxidant activity) at the local level (in bile) in patients with bile duct obstruction of malignant etiology. Constellation of metabolic disorders allowed to propose an original method for determining the lithogenicity of bile (integral indicator of lithogenicity). The obtained data made it possible to justify the need to assess the degree of metabolic disturbances in bile, on the basis of which it is possible to predict the risk of lithogenesis and the duration of stent operation, which in turn helped optimize the algorithm for choosing the method of bile ducts decompression aimed at preventing early obturation of endoprostheses.

[Optimization of reparative dentinogenesis in experimental osteoporosis]. / Optimizatsiia reparativnogo dentinogeneza pri éksperimental'nom osteoporoze.

The aim of the study was to assess histochemical changes of the dental pulp in direct pulp capping/experimental osteoporosis animal model. The study was performed on 20 two-year sheep with simulated acute pulpitis divided in 2 groups: main (15 animals/120 teeth) and control (5 animals/40 teeth). Direct pulp capping in the main group included tissue-engineered structure composed of a hydrogel PuraMatrix/3DM with ectomesenchymal stem cells immobilized on collagen sponge. In the control group collagen sponges with hydrocortisone furatsilin, chondroitin sulfate, аnaesthesinum were used for the same purpose. Dentinal bridge formation was much slower in controls than in the main group. Developed tissue-engineered design optimizes each stage of the healing process by protecting the pulp from infection, reduction of exudation, hemostatic effect and in long term contributes to a significant acceleration of the formation of the dentinal bridge.

[Influence of linseed oil and deiterium depleted water on isotopic D/H composition and functional antioxidant defense of the hepatobiliary system in rabbits with carbon tetrachloride intoxication].

The article presents results of studying the influence of linseed oil and drinking diet with a modified isotopic composition with low deuterium content on indicators of prooxidant-antioxidant system during modeling of liver toxicity. The research was performed on 36 rabbits (weighing 3.1-3.5 kg) which were divided into 4 groups. Group 1 consisted of control animals; in group 2, 3 and 4 in rabbits the liver toxicity was modeled by administration of CCl4 (intraperitoneally, in the form of a 50% oil solution, 1 ml per kg bw, 2 times a week for 30 days); nutritional correction using flaxseed oil (0.1 ml per 100 g bw) and drinking diet with deuterium depleted water (50 ppm) was carried out in animals from groups 3 and 4 respectively, for 30 days prior to simulation of toxic hepatitis and more throughout the experiment. Using the method of nuclear magnetic resonance and mass spectrometry the influence of deuterium depleted water on D/H composition of the blood plasma, bile and liver tissues was determined: the deuterium concentration in these biological materials significant decreased. The most significant decrease in the deuterium content (30.2% compared with the control group) was found in bloodplasma in animals from group 4. The study of the state of prooxidant-antioxidant balance of the liver and bile showed oxidative stress at the local level, with the toxic effects of carbon tetrachloride. This was followed by EPR spectroscopy data pronounced increase of the number of paramagnetic centers in the hepatocytes by 5.4, 1.9 and 2.8 fold in animals of 2, 3 and 4 groups, respectively (compared to the indicators of the first group). There was also increase in the intensity of free radical oxidation processes in the bile with a simultaneous reduction of its antioxidant activity, which was significantly less distinct (on average 51.18-59.8%, p<0.05) in animals treated with nutritional correction, indicating that higher functional activity of protective systems involved in recycling prooxidant factors using dietary lipophilic antioxidants and water with low deuterium content. Overall, the results of the present study indicate that existing in the liver and bile autonomous mechanisms of regulation of the state of prooxidant-antioxidant systems are quite sensitive to the effects of antioxidant factors of lipophilic nature and shifts of isotopic D/H gradient, and suggest usefulness of the products that can affect these indicators to increase adaptive capabilities of the organism during intoxication.

[Influence of deuterium depleted water on the organism of laboratory animals in various functional conditions of nonspecific protective systems].

The influence of deuterium depleted water with the modified isotope composition on an organism of animals is studied in physiological conditions and development of chronic endogenous intoxication of hepatorenal genesis. The influence of this water on isotope composition of plasma and tissues (the liver and kidneys) in laboratory animals is shown. The impact of this water on biochemical indicators (AST, ALT, alkaline phosphatase, creatinine, bilirubin) and dynamics in body weight of laboratory animals within 42 days is established. As a result of studies the possibility of the preventive use of deuterium depleted water for correction of metabolic processes is shown in various conditions of the functional system of the body's detoxification.

[Comparative evaluation of antioxidant activity and content of prooxidant factors in different classes of foods].

By using the biophysical methods (chemiluminescence, amperometry) in laboratory in vitro experiments it was demonstrated that the study of antioxidant and pro-oxidant activities of different food groups allows to perform a preliminary assessment of their pro-oxidant-antioxidant capacity. It have been shown that some food prevails ability to exert pro-oxidant effects (in vitro) due to the short-term induction of free radical oxidation. Thus, among the fresh juices the increase of the maximum of flash chemiluminescence has been detected in avocado (1080, 89%) and pearjuices (136,33%), whereas the lowest ability to enhance the intensity of free radical processes has been marked for pomegranate (1,63%), orange (9, 68%) and apples juices (12, 84%). Among milk products it has been marked for sour milk (9, 06%) and yogurt (15, 11-16,02%), that allows the use of the past to correct pro-oxidant-antioxidant balance diet for people with potential danger gain peroxide processes, such as special physiological states, sport endurance, mental and emotional overload. The ability to increase the intensity of free radical oxidation have been also identified for snacks, especially buns, biscuits, bread sticks, showing the risk of formation of oxidative stress in the body during their prolonged use, particularly under the above described conditions. In some cases, foods (processed cheese and cheese curds) showed dominance factors sustained oxidative effect (in 2,1-20,7%), that indicates the possibility of an imbalance in the pro-oxidant-antioxidant system after its prolonged use in the diet, even in small quantities, especially in individuals with a reduced level of antioxidant potential of the nonspecific defense system. Investigation of antioxidant activity of foods revealed significant predominance of reducing equivalents in all freshly squeezed and some packaged fruit juices, as well as dairy products, indicating their possibility to increase the capacity of reducing components of nonspecific protection system. The presence in a number of products used for snacking (chips, puffed rice) of sufficiently high levels of antioxidant activity demonstrates the content of substances that act as proton donator having significant reducing ability. It is sufficient for high energy value of such products, indicating on the possibility of involvement of their recovery factors in plastic processes with subsequent enhancement, such as lipogenesis, that may be a prerequisite for the development of obesity, atherosclerosis and otherpathological processes. Using an integrated approach will enable a timely and appropriate to correct the ratio of pro- and antioxidant indicators of diet in order to improve the adaptive capacity of the organism in a particular physiological or pathological conditions involving oxidative metabolism disorders.

[Determination of deuterium concentration in foods and influence of water with modified isotopic composition on oxidation parameters and heavy hydrogen isotopes content in experimental animals].

The article presents the results of the study of the deuterium (D) content in food products as well as the influence of deuterium depleted water (DDW) on the concentration of heavy hydrogen isotopes in the blood and lyophilized tissues of rats. The most significant difference in the content of D was found between potato and pork fat, which indexes the standard delta notation (δ) D in promille, related to the international standard SMOW (Standard Mean Ocean of Water) amounted to -83,2 per thousand and -250,7 per thousand, respectively (p<0,05). Among the investigated samples of water deuterium concentration ranged from -75,5 per thousand (Narzan) to +72,1 per thousand (Kubai), that indicates the ability of some food products to increase the concentration of heavy hydrogen atoms in the body. The data obtained in the experimental modeling of the diet of male Wistar rats in the age of 5-6 mo (weight 235 ± 16 g) using DDW (δD = -743,2 per thousand) instead of drinking water (δD = -37,0 per thousand) with identical mineral composition showed that after 2 weeks significant (p <0,05) formation of isotopic (deuterium-protium, D/H) gradient in the body is possible. Changing the direction of isotopic D/H gradient in laboratory animals in comparison with its physiological indicators (72-127 per thousand, "plasma>>tissue") is due to different rates ofisotopic exchange reactions in plasma and tissues (liver, kidney, heart), which can be explained by entering into the composition of a modified diet of organic substrates with more than DDW concentration D, which are involved in the construction of cellular structures and eventually lead to a redistribution of D and change direction of D/H gradient "plasma<

[Allelic imbalance of loci 17p13.1 (TP53), 1p36.1 (RUNX3), 16p22 (CDH1) and microsatellite instability in gastric cancer].

We examined allelic imbalance (AI) on loci 17p13.1 (TP53), 1p36.1 (RUNX) and 16p22 (CDHI) and microsatellite instability (MI) with BAT26 in 78 patients with gastric cancer. We have shown a significant difference in the frequency of allelic imbalance of the studied loci among different types of gastric cancer. Frequency of AI in 16p22.1 (CDH1) (p = 0.023), 17p13.1 (TP53) (p = 0.038), microsatellite instability (p = 0.047) and AD two and more loci in a single sample (p = 0.0176) was significantly higher in the intestinal type of gastric cancer than in the diffuse type. We have shown, that, frequency of AI in 16p22.1 (CDH1), and AD two and more loci in a single sample, was higher in thetumors with high or moderate type of tumor cells differentiation (p = 0.0414, p = 0.0057 respectively). We found no significant differences in the groups with metastases in regional lymph nodes, different tumor stage, localization of tumors and the generalization process.

[Comparative characteristics of antioxidant capacity and energy content of some foods].

The paper presents a comparative evaluation of antioxidant capacity and energy values of different foods groups in order to identify the most efficient combinations for correction of metabolic disorders associated with an imbalance in antioxidant system. In study integral method for determining of antioxidant and energy indicators (patent No 2,455,703) has been used. It has been revealed that the highest antioxidant-energy capacity (AE) of fresh juices has a pomegranate juice (AE = 3895.9 +/- 241.4 mg/L x kJ(-1)), other fresh juices inferior to him on this indicator: grenade>orange>lemon=apple> pomelo > mandarin > persimmon > kiwi > pears > avocado. Among dairy products the highest AE belongs to boiled fermented milk--"ryazhenka" (AE = 40.9 +/- 2.7 mg/L x kJ(-1)), other dairy products can be placed in line with index AE: ryazhenka ==>kefir > yogurt. Most of fresh juices were significantly superior to antioxidant-energy potential of other foods. Despite the fact that dairy products AE were lower than AE of some juices, they were much superior to AE values of fastfood products (biscuits, potato chips, popcorn). This demonstrates need to reduce the quota of fast foods in the diet to prevent the risk of reduction potential of the endogenous antioxidant system.

[Change of blood antioxidant capacity of experimental animals during nutritional correction under oxidative stress].

The effect of nutritional correction (a diet high in foods with antioxidant content) on blood parameters in laboratory animals with metabolic disorders associated with oxidative stress has been studied. In experimental models of laboratory animals (male rabbits weighing 3.5-4.0 kg, n = 40) with purulent septic diseases it has been demonstrated that the use of nutritive correction (replacement of 100 g of the cereal mixture through day on a mixture of cabbage 50 g, carrots 50 g, beet 25 g, apple 25 g, kiwi 10 g and garnet 10 g per 1 rabbit) is not inferior to its efficiency of glutathione use (2 g per day). The use of these antioxidants in laboratory animals significantly reduced the phenomenon of oxidative stress on the 5th day: blood antioxidant capacity significantly increased by 14.9 and 26.6%, and the area of the flash of luminol-dependent H2O2-induced chemiluminescence of blood plasma reduced by 44.2 and 48.6% in the experimental groups receiving respectively nutritive correction and glutathione. The low-molecula level of blood antioxidant capacity was restored and the balance of the activity of superoxide dismutase (decrease) and catalase (increase) was achieved on the 10th day of the experiment. These figures significantly (p < 0.05) differed from than in the group of animals receiving no antioxidant correction. The latter studied parameters of prooxidant-antioxidant system reached values comparable with those in intact animals (n = 10) only on the 30th day, confirming the advisability of appointing a complex antioxidant therapy.

[The effect of carbon tetrachloride poisoning on the activity of digestive proteases in rats and correction of the disorders with vegetable oils].

The results of the study of activity of digestive proteases (pepsin, trypsin, chymotrypsin) in homogenates of stomach, pancreas and duodenum in experimental animals have been presented. Rats were exposed to intoxication with carbon tetrachloride (subcutaneous administration of a 50% oil solution of CCl4 in the dose of 0.5 ml per 100 g body weight) for three days and then they were given analysed oils (black nut, walnut and flax oil) intragastrically by gavage at a dose of 0.2 ml per day within 23 days. Pepsin level in gastric mucosa homogenates and chymotrypsin activity in pancreatic homogenates were determined by method of N.P. Pyatnitskiy based on on the ability of enzymes to coagulate dairy-acetate mixture, respectively, at 25 degrees C and 35 degrees C. Trypsin activity in homogenates of pancreatic was determined by method of Erlanger - Shaternikova colorimetrically. It has been established that intoxication with CCl4 decreased the synthesis of proteolytic enzymes of the stomach (by 51%) and pancreas (by 70-78%). Injections of analysed vegetable oils to animals contributed to the normalization of proteolytic enzymes synthesis. The conclusion that there are prospects of using the analysed vegetable oils containing large quantity of polyunsaturated fatty acids (omega-3 and omega-6) for the correction of detected biochemical abnormalities has been done.

[The systems of molecular genetic markers under cancer of stomach].

The study was organized to investigate the anomalous methylation of genes NA?1, RASSF1A, MLH1, N33, DAPK, the expression of genes hTERT. metalloproteinase MMP7, MMP9, survivin. COX-2, p53. The activity of telomerase in 106 samples of stomach tumors taken through intra-operation way and 53 samples of stomach tumors taken through endoscopic way and 50 samples of biopsy taken from patients with chronic calculous cholecystitis (comparison group) was analyzed too. These changes can be used as additional markers both in diagnostic of cancer of stomach and dynamic monitoring of operated patients.