Do "brassy" sounding musical instruments need increased safe distancing requirements to minimize the spread of COVID-19?

Brass wind instruments with long sections of cylindrical pipe, such as trumpets and trombones, sound "brassy" when played at a fortissimo level due to the generation of a shock front in the instrument. It has been suggested that these shock fronts may increase the spread of COVID-19 by propelling respiratory particles containing the SARS-CoV-2 virus several meters due to particle entrainment in the low pressure area behind the shocks. To determine the likelihood of this occurring, fluorescent particles, ranging in size from 10-50 µm, were dropped into the shock regions produced by a trombone, a trumpet, and a shock tube. Preliminary results indicate that propagation of small airborne particles by the shock fronts radiating from brass wind instruments is unlikely.

A simple and inexpensive optical technique to help students visualize mode shapes.

An imaging technique is introduced that is suitable for visualizing the mode shapes of vibrating structures in an educational setting. The method produces images similar to those obtained using electronic speckle pattern interferometry (ESPI), but it can be implemented for less than 1/10 the cost of a commercial ESPI system, and the apparatus is simple enough that it can be constructed by undergraduate students. This technique allows for real-time visualization of the normal modes and deflection shapes of harmonically vibrating structures, including those with shapes that make generating Chladni patterns with sand or powder impossible. The theory of operation and construction details are discussed.

Tuning the Nigerian slit gong.

An experimental and theoretical investigation of the Nigerian slit gong is reported. It is shown that in tuning the gong the artisan ensures that the frequencies of the two lowest mechanical resonances are nearly coincident with the frequencies of two of the acoustic resonances of the internal cavity. Four possible tuning parameters are identified and the effects of changing these parameters are discussed.

A mobile angular scattering microscope for organelle size estimation.

Angular light scattering measurements have been used to determine the size parameters of spherical particles. By measuring the angular scattering from biological specimen, the average size of the cellular organelles can be estimated, which can be used to determine information about the health of the biological sample. An angular scattering microscope with the ability to be easily moved was constructed from common inexpensive components, which has potential applications for clinical and low-resource settings. The stability and accuracy of the system were investigated by measuring the scattering from polystyrene beads with mean sizes of 5 and 1.75 µm with narrow size distributions. Resulting size estimates obtained from the scattering patterns were consistent with the manufacturer-specified range of diameters for each sample. Initial studies were also conducted on individual fixed HeLa cells. The results presented indicate that the system is capable of obtaining precise and accurate size estimates of beads and single cells' organelles.

Imaging of vibrating objects using speckle subtraction.

A simple method for imaging vibrational motion is proposed. The process consists of capturing two speckled images of a region illuminated by coherent radiation. One of the images is captured before the onset of motion and the other during motion. If the mean speckle intensity is below the threshold for detection or above the saturation intensity of the detector, subtracting the two images produces a high contrast image of the moving region. A theory is shown to agree well with experimental data.

Angularly resolved, finely sampled elastic scattering measurements of single cells: requirements for robust organelle size extractions.

Angularly resolved elastic light scattering is an established technique for probing the average size of organelles in biological tissue and cellular ensembles. Focusing of the incident light to illuminate no more than one cell at a time restricts the minimum forward-scattering angle θmin that can be detected. Series of simulated single-cell angular-scattering patterns have been generated to explore how size estimates vary as a function of θmin. At a setting of θmin = 20 deg, the size estimates hop unstably between multiple minima in the solution space as simulated noise (mimicking experimentally observed levels) is varied. As θmin is reduced from 20 deg to 10 deg, the instability vanishes, and the variance of estimates near the correct answer also decreases. The simulations thus suggest that robust Mie theory fits to single-cell scattering at 785 nm excitation require measurements down to at least 15 deg. Notably, no such instability was observed at θmin = 20 deg for narrow bead distributions. Accurate sizing of traditional calibration beads is, therefore, insufficient proof that an angular-scattering system is capable of robust analysis of single cells. Experimental support for the simulation results is also presented using measurements on cells fixed with formaldehyde.

Methodology for high-yield acquisition of functional near-infrared spectroscopy data from alert, upright infants.

Functional near-infrared spectroscopy (fNIRS) research to date has tended to publish group-averaged rather than individual infant data due to normative basic research goals. Acquisition of individual infant time courses holds interest, however, both for cognitive science and particularly for clinical applications. Infants are more difficult to study than adults as they cannot be instructed to remain still. In addressing this, upright infants pose several associated complications for the researcher. We identified and optimized the factors that affect the quality of fNIRS data from individual 6- to 9-month-old infants exposed to a visual stimulation paradigm. The fNIRS headpiece was reconfigured to reduce inertia, increase comfort, and improve conformity to the head, while preserving fiber density to avoid missing the visual cortex activation. The visual-stimulation protocol was modified to keep the attention of infants throughout the measurement, thus helping to reduce motion artifacts. Adequate optical contact was verified by checking power levels before each measurement. By revising our experimental process and our data rejection criteria to prioritize good optical contact, we report for the first time usable hemodynamic data from 83% of infants and that two-thirds of infants produced a statistically significant fNIRS response.