RESUMO
An automated water-renewal toxicity test system is described for exposing benthic invertebrates to whole sediments. The system will intermittently deliver laboratory or on-site water for overlying water replacement in sediment exposures. A range of cycle rates can be used to produce different volume additions of overlying water per day to exposure chambers. The system can be used with six different treatments and eight replicates per treatment producing 48 exposure chambers. Three formulated sediments with variable organic carbon (1.5%, 7.5%) and sand (14%, 63%) content were prepared to test the system exposing amphipods, Hyalella azteca and midges, Chironomus tentans in 10 day whole sediment tests. Intermittent water flow was used with a 90 min cycle time to create two volume additions of laboratory water per 24 h in exposure chambers (180 ml sediment, 320 ml water). Overlying water quality conditions, and survival and growth of both species were consistent and within acceptable limits for the testing requirements of the U.S. EPA guidelines for sediments with freshwater invertebrates.
Assuntos
Monitoramento Ambiental/instrumentação , Sedimentos Geológicos , Plâncton , Testes de Toxicidade/instrumentação , Animais , Automação , Monitoramento Ambiental/métodos , Água Doce , Testes de Toxicidade/métodosRESUMO
U.S. federal legislation relevant to the coastal zone and adjacent ocean was reviewed and included the Coastal Zone Management Act, Clean Water Act, Marine Protection Research and Sanctuaries Act, and Oil Pollution Act. Problems affecting these waters involve complex activities/issues related to multimedia sources of pollution and coastal land use planning. Effective pollution control requires state-local-federal interaction with a better understanding of scientific as well as social and economic issues.