RESUMO
Context: The chronic diabetes mellitus (DM) condition may lead to diabetic wounds that increase morbidity in patients. Ipomoea reptans Poir leaves have been widely reported to possess anti-diabetic activity due to their flavonoid contents. To enhance drug penetration, a nanoemulgel preparation was formulated. Aims: This study aimed to evaluate the activity of nanoemulgel preparations of Ipomoea reptans Poir leaf extract on diabetic and non-diabetic wound-healing using male Wistar rats. Settings and Design: This research was an experimental study with a post-test only control group design. Materials and Methods: The rats (n = 32) were randomly divided into two groups: diabetic (induced by 40 mg/kg BW STZ) and non-diabetic model. Each model consisted of four groups: normal, positive control, I. reptans leaf extract (IRLE), and nanoemulgel of I. reptans leaf extract (NIRLE). All the animals studied were shaved from the back, and a 2.5 × 0.5 cm full-thickness excision wound was made. IRLE and NIRLE were administered daily and observed for the wound-healing process. Statistical Analysis Used: The one-way analysis of variance with the Tukey post-hoc test was used for the statistical analysis. Results: A NIRLE formulation has been developed to produce a preparation that meets the physical requirements. IRLE and NIRLE possessed wound-healing activity in normal and diabetic rat models. However, the wound-healing process in diabetic rats treated with NIRLE was faster than those with IRLE. Conclusions: NIRLE increased the activity of wound-healing effect of I. reptans leaves on diabetic rats in comparison with the extract form.
RESUMO
A fluorescence probe based on iron oxide quantum dots (IO-QDs) was synthesized using the hydrothermal method for the determination of tetracycline (TCy) and ciprofloxacin (CPx) in aqueous solution. The IO-QDs were characterized using high-resolution transmission electron microscopy (HR-TEM), powder X-ray diffraction (P-XRD), vibrating sample magnetometry (VSM), and Fourier-transform infrared spectroscopy (FTIR). The as-prepared IO-QDs are fluorescent, stable, and with a fluorescence quantum yield (QY) of 9.8 ± 0.12%. The fluorescence of IO-QDs was observed to be quenched and enhanced in the presence of TCy and CPx, respectively. The fluorescence intensity ratio shows linearity at concentrations from 1-100 µM and 5-100 µM for TCy and CPx, respectively; the detection limit for TCy and CPx was estimated to be 0.71 µM and 1.56 µM, respectively. The proposed method was also successfully utilized in the spiked samples of drinking water and honey with good recoveries. The method offered convenience, rapid detection, high sensitivity, selectivity, and cost-efficient alternative options for the determination of TCy and CPx in real samples.