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1.
Nucleic Acids Res ; 51(20): 11291-11297, 2023 11 10.
Artigo em Inglês | MEDLINE | ID: mdl-37811879

RESUMO

There are >170 naturally occurring RNA chemical modifications, with both known and unknown biological functions. Analytical methods for detecting chemical modifications and for analyzing their effects are relatively limited and have had difficulty keeping pace with the demand for RNA chemical biology and biochemistry research. Some modifications can affect the ability of RNA to hybridize with its complementary sequence or change the selectivity of base pairing. Here, we investigate the use of affinity-based DNA nanoswitches to resolve energetic differences in hybridization. We found that a single m3C modification can sufficiently destabilize hybridization to abolish a detection signal, while an s4U modification can selectively hybridize with G over A. These results establish proof of concept for using DNA nanoswitches to detect certain RNA modifications and analyzing their effects in base pairing stability and specificity.


Assuntos
DNA , RNA , Pareamento de Bases , Sequência de Bases , DNA/química , Hibridização de Ácido Nucleico , RNA/química
2.
Nucleic Acids Res ; 51(8): 4055-4063, 2023 05 08.
Artigo em Inglês | MEDLINE | ID: mdl-36477864

RESUMO

The ability to create stimuli-responsive DNA nanostructures has played a prominent role in dynamic DNA nanotechnology. Primary among these is the process of toehold-based strand displacement, where a nucleic acid molecule can act as a trigger to cause conformational changes in custom-designed DNA nanostructures. Here, we add another layer of control to strand displacement reactions through a 'toehold clipping' process. By designing DNA complexes with a photocleavable linker-containing toehold or an RNA toehold, we show that we can use light (UV) or enzyme (ribonuclease) to eliminate the toehold, thus preventing strand displacement reactions. We use molecular dynamics simulations to analyze the structural effects of incorporating a photocleavable linker in DNA complexes. Beyond simple DNA duplexes, we also demonstrate the toehold clipping process in a model DNA nanostructure, by designing a toehold containing double-bundle DNA tetrahedron that disassembles when an invading strand is added, but stays intact after the toehold clipping process even in the presence of the invading strand. This work is an example of combining multiple physical or molecular stimuli to provide additional remote control over DNA nanostructure reconfiguration, advances that hold potential use in biosensing, drug delivery or molecular computation.


Assuntos
DNA , Nanoestruturas , DNA/química , Nanotecnologia , RNA , Simulação de Dinâmica Molecular
3.
J Am Chem Soc ; 145(6): 3599-3605, 2023 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-36731121

RESUMO

Reconfigurable structures engineered through DNA hybridization and self-assembly offer both structural and dynamic applications in nanotechnology. Here, we have demonstrated that strand displacement of triplex-forming oligonucleotides (TFOs) can be translated to a robust macroscopic DNA crystal by coloring the crystals with covalently attached fluorescent dyes. We show that three different types of triplex strand displacement are feasible within the DNA crystals and the bound TFOs can be removed and/or replaced by (a) changing the pH from 5 to 7, (b) the addition of the Watson-Crick complement to a TFO containing a short toehold, and (c) the addition of a longer TFO that uses the duplex edge as a toehold. We have also proved by X-ray diffraction that the structure of the crystals remains as designed in the presence of the TFOs.


Assuntos
DNA , Oligonucleotídeos , DNA/química , Oligonucleotídeos/química , Hibridização de Ácido Nucleico , Corantes Fluorescentes , Conformação de Ácido Nucleico
4.
Small ; 19(39): e2300040, 2023 09.
Artigo em Inglês | MEDLINE | ID: mdl-37264756

RESUMO

The programmable nature of DNA allows the construction of custom-designed static and dynamic nanostructures, and assembly conditions typically require high concentrations of magnesium ions that restricts their applications. In other solution conditions tested for DNA nanostructure assembly, only a limited set of divalent and monovalent ions are used so far (typically Mg2+ and Na+ ). Here, we investigate the assembly of DNA nanostructures in a wide variety of ions using nanostructures of different sizes: a double-crossover motif (76 bp), a three-point-star motif (~134 bp), a DNA tetrahedron (534 bp) and a DNA origami triangle (7221 bp). We show successful assembly of a majority of these structures in Ca2+ , Ba2+ , Na+ , K+ and Li+ and provide quantified assembly yields using gel electrophoresis and visual confirmation of a DNA origami triangle using atomic force microscopy. We further show that structures assembled in monovalent ions (Na+ , K+ and Li+ ) exhibit up to a 10-fold higher nuclease resistance compared to those assembled in divalent ions (Mg2+ , Ca2+ and Ba2+ ). Our work presents new assembly conditions for a wide range of DNA nanostructures with enhanced biostability.


Assuntos
Nanoestruturas , Nanotecnologia , Nanotecnologia/métodos , Conformação de Ácido Nucleico , Nanoestruturas/química , DNA/química , Cátions
5.
EMBO Rep ; 22(6): e53024, 2021 06 04.
Artigo em Inglês | MEDLINE | ID: mdl-33969617

RESUMO

Giving undergraduate students an opportunity to partake in a research project pays back for both students and the lab.


Assuntos
Projetos de Pesquisa , Estudantes , Humanos
6.
J Chem Educ ; 100(1): 316-320, 2023 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-37193549

RESUMO

The field of DNA nanotechnology has grown rapidly in the last decade and has expanded to multiple laboratories. While lectures in DNA nanotechnology have been introduced in some institutions, laboratory components at the undergraduate level are still lacking. Undergraduate students predominantly learn about DNA nanotechnology through their involvement as interns in research laboratories. The DNA nanostructure biostability analysis experiment presented here can be used as a hands-on introductory laboratory exercise for discussing concepts in DNA nanotechnology in an undergraduate setting. This experiment discusses biostability, gel electrophoresis and quantitative analysis of nuclease degradation of a model DNA nanostructure, the paranemic crossover (PX) DNA motif. The experiment can be performed in a chemistry, biology or a biochemistry laboratory with minimal costs and can be adapted in undergraduate institutions using the instructor and student manuals provided here. Laboratory courses based on cutting edge research not only provide students a direct hands-on approach to the subject, but can also increase undergraduate student participation in research. Moreover, laboratory courses that reflect the increasingly multidisciplinary nature of research add value to undergraduate education.

7.
Trends Biochem Sci ; 43(12): 997-1013, 2018 12.
Artigo em Inglês | MEDLINE | ID: mdl-30342801

RESUMO

Simple base-pairing rules of complementarity, perfected by evolution for encoding genetic information, provide unprecedented control over the process of DNA self-assembly. These rules allow us to build exquisite nanostructures and rationally design their morphology, fine-tune their chemical properties, and program their response to environmental stimuli. DNA nanostructures have emerged as promising candidates for transporting drugs across various physiological barriers of the body. In this review, we discuss the strategies used to transform DNA nanostructures into drug delivery vehicles. We provide an overview of recent attempts at using them to deliver small molecule drugs and macromolecular cargoes and present the challenges that lay ahead for these synthetic vectors as they set new paradigms in the field of nanotechnology and medicine.


Assuntos
DNA/química , Nanoestruturas/química , Sistemas de Liberação de Medicamentos/métodos , Nanotecnologia/métodos
8.
RNA ; 26(9): 1118-1130, 2020 09.
Artigo em Inglês | MEDLINE | ID: mdl-32414856

RESUMO

Polyriboadenylic [poly(rA)] strands of sufficient length form parallel double helices in acidic and/or ammonium-containing conditions. Poly(rA) duplexes in acidic conditions are held together by A+-A+ base-pairing also involving base interactions with the phosphate backbone. Traditional UV-melting studies of parallel poly(A) duplexes have typically examined homo-duplex formation of a single nucleic acid species in solution. We have adapted a technique utilizing a DNA nanoswitch that detects interaction of two different strands either with similar or differing lengths or modifications. Our method detected parallel duplex formation as a function of length, chemical modifications, and pH, and at a sensitivity that required over 100-fold less concentration of sample than prior UV-melting methods. While parallel polyriboadenylic acid and poly-2'-O-methyl-adenylic acid homo-duplexes formed, we did not detect homo-duplexes of polydeoxyriboadenylic acid strands or poly-locked nucleic acid (LNA)-adenylic strands. Importantly however, a poly-locked nucleic acid (LNA)-adenylic strand, as well as a poly-2'-O-methyl-adenylic strand, formed a hetero-duplex with a polyriboadenylic strand. Overall, our work validates a new tool for studying parallel duplexes and reveals fundamental properties of poly(A) parallel duplex formation. Parallel duplexes may find use in DNA nanotechnology and in molecular biology applications such as a potential poly(rA) tail capture tool as an alternative to traditional oligo(dT) based purification.


Assuntos
DNA/genética , Poli A/genética , Pareamento de Bases/genética , Conformação de Ácido Nucleico , Oligonucleotídeos/genética
9.
Nano Lett ; 21(1): 469-475, 2021 01 13.
Artigo em Inglês | MEDLINE | ID: mdl-33395311

RESUMO

Molecular biomarkers play a key role in the clinic, aiding in diagnostics and prognostics, and in the research laboratory, contributing to our basic understanding of diseases. Detecting multiple and diverse molecular biomarkers within a single accessible assay would have great utility, providing a more comprehensive picture for clinical evaluation and research, but is a challenge with standard methods. Here, we report programmable DNA nanoswitches for multiplexed detection of up to 6 biomarkers at once with each combination of biomarkers producing a unique barcode signature among 64 possibilities. As a defining feature of our method, we show "mixed multiplexing" for simultaneous barcoded detection of different types of biomolecules, for example, DNA, RNA, antibody, and protein in a single assay. To demonstrate clinical potential, we show multiplexed detection of a prostate cancer biomarker panel in serum that includes two microRNA sequences and prostate specific antigen.


Assuntos
DNA , MicroRNAs , Biomarcadores Tumorais/genética , DNA/genética , MicroRNAs/genética
11.
Biochemistry ; 60(4): 250-253, 2021 02 02.
Artigo em Inglês | MEDLINE | ID: mdl-33464826

RESUMO

Nanoscale devices that can respond to external stimuli have potential applications in drug delivery, biosensing, and molecular computation. Construction using DNA has provided many such devices that can respond to cues such as nucleic acids, proteins, pH, light, or temperature. However, simultaneous control of molecular devices is still limited. Here, we present orthogonal control of DNA nanoswitches using physical (light) and biochemical (enzyme and nucleic acid) triggers. Each one of these triggers controls the reconfiguration of specific nanoswitches from locked to open states within a mixture and can be used in parallel to control a combination of nanoswitches. Such dynamic control over nanoscale devices allows the incorporation of tunable portions within larger structures as well as spatiotemporal control of DNA nanostructures.


Assuntos
DNA/química , Nanoestruturas/química , Conformação de Ácido Nucleico
12.
Chem Rev ; 119(22): 11631-11717, 2019 11 27.
Artigo em Inglês | MEDLINE | ID: mdl-31573184

RESUMO

Development of biosensing platforms plays a key role in research settings for identification of biomarkers and in clinical applications for diagnostics. Biosensors based on nucleic acids have taken many forms, from simple duplex-based constructs to stimuli-responsive nucleic acid nanostructures. In this review, we look at various nucleic acid-based biosensors, the different read-out strategies employed, and their use in chemical and biological sensing. We also look at current developments in DNA nanotechnology-based biosensors and how rational design of such constructs leads to more efficient biosensing platforms.


Assuntos
Técnicas Biossensoriais/métodos , Ácidos Nucleicos/química , Animais , Técnicas Biossensoriais/instrumentação , DNA/química , Transferência Ressonante de Energia de Fluorescência/métodos , Humanos , Modelos Moleculares , Conformação de Ácido Nucleico
13.
Chem Rev ; 119(10): 6273-6289, 2019 05 22.
Artigo em Inglês | MEDLINE | ID: mdl-29911864

RESUMO

Over the past 35 years, DNA has been used to produce various nanometer-scale constructs, nanomechanical devices, and walkers. Construction of complex DNA nanostructures relies on the creation of rigid DNA motifs. Paranemic crossover (PX) DNA is one such motif that has played many roles in DNA nanotechnology. Specifically, PX cohesion has been used to connect topologically closed molecules, to assemble a three-dimensional object, and to create two-dimensional DNA crystals. Additionally, a sequence-dependent nanodevice based on conformational change between PX and its topoisomer, JX2, has been used in robust nanoscale assembly lines, as a key component in a DNA transducer, and to dictate polymer assembly. Furthermore, the PX motif has recently found a new role directly in basic biology, by possibly serving as the molecular structure for double-stranded DNA homology recognition, a prominent feature of molecular biology and essential for many crucial biological processes. This review discusses the many attributes and usages of PX-DNA-its design, characteristics, applications, and potential biological relevance-and aims to accelerate the understanding of PX-DNA motif in its many roles and manifestations.


Assuntos
DNA/química , Nanotecnologia/métodos , Modelos Moleculares , Nanotecnologia/instrumentação , Conformação de Ácido Nucleico
14.
Nucleic Acids Res ; 47(20): 10489-10505, 2019 11 18.
Artigo em Inglês | MEDLINE | ID: mdl-31287874

RESUMO

MicroRNAs are involved in the crucial processes of development and diseases and have emerged as a new class of biomarkers. The field of DNA nanotechnology has shown great promise in the creation of novel microRNA biosensors that have utility in lab-based biosensing and potential for disease diagnostics. In this Survey and Summary, we explore and review DNA nanotechnology approaches for microRNA detection, surveying the literature for microRNA detection in three main areas of DNA nanostructures: DNA tetrahedra, DNA origami, and DNA devices and motifs. We take a critical look at the reviewed approaches, advantages and disadvantages of these methods in general, and a critical comparison of specific approaches. We conclude with a brief outlook on the future of DNA nanotechnology in biosensing for microRNA and beyond.


Assuntos
DNA/química , MicroRNAs/análise , Técnicas de Diagnóstico Molecular/métodos , Nanotecnologia/métodos , Animais , Humanos , Nanopartículas/química
15.
J Am Chem Soc ; 142(14): 6814-6821, 2020 04 08.
Artigo em Inglês | MEDLINE | ID: mdl-32208657

RESUMO

Nanometer-sized features and molecular recognition properties make DNA a useful material for nanoscale construction, but degradation in biological fluids poses a considerable roadblock to biomedical applications of DNA nanotechnology. Here, we report the remarkable biostability of a multistranded motif called paranemic crossover (PX) DNA. Compared to double stranded DNA, PX DNA has dramatically enhanced (sometimes >1000 fold) resistance to degradation by four different nucleases, bovine and human serum, and human urine. We trace the cause of PX's biostability to DNA crossovers, showing a continuum of protection that scales with the number of crossovers. These results suggest that enhanced biostability can be engineered into DNA nanostructures by adopting PX-based architectures or by strategic crossover placement.


Assuntos
DNA/química , Nanotecnologia/métodos , Humanos , Modelos Moleculares , Motivos de Nucleotídeos
16.
Nucleic Acids Res ; 46(3): 1021-1037, 2018 02 16.
Artigo em Inglês | MEDLINE | ID: mdl-29228337

RESUMO

DNA self-assembly has proved to be a useful bottom-up strategy for the construction of user-defined nanoscale objects, lattices and devices. The design of these structures has largely relied on exploiting simple base pairing rules and the formation of double-helical domains as secondary structural elements. However, other helical forms involving specific non-canonical base-base interactions have introduced a novel paradigm into the process of engineering with DNA. The most notable of these is a three-stranded complex generated by the binding of a third strand within the duplex major groove, generating a triple-helical ('triplex') structure. The sequence, structural and assembly requirements that differentiate triplexes from their duplex counterparts has allowed the design of nanostructures for both dynamic and/or structural purposes, as well as a means to target non-nucleic acid components to precise locations within a nanostructure scaffold. Here, we review the properties of triplexes that have proved useful in the engineering of DNA nanostructures, with an emphasis on applications that hitherto have not been possible by duplex formation alone.


Assuntos
DNA/química , Nanoestruturas/química , Nanotecnologia/métodos , Oligodesoxirribonucleotídeos/química , Aptâmeros de Nucleotídeos/síntese química , Aptâmeros de Nucleotídeos/química , Pareamento de Bases , Sequência de Bases , Técnicas Biossensoriais , DNA/genética , Engenharia Genética/métodos , Humanos , Concentração de Íons de Hidrogênio , Conformação de Ácido Nucleico , Oligodesoxirribonucleotídeos/genética
17.
J Chem Educ ; 97(5): 1448-1453, 2020 May 12.
Artigo em Inglês | MEDLINE | ID: mdl-33814597

RESUMO

There is a disconnect between the cutting-edge research done in academic labs, such as nanotechnology, and what is taught in undergraduate labs. In the current undergraduate curriculum, very few students get a chance to do hands-on experiments in nanotechnology-related experiments most of which are through selective undergraduate research programs. In most cases, complicated synthesis procedures, expensive reagents, and requirement of specific instrumentation prevent broad adaptation of nanotechnology-based experiments to laboratory courses. DNA, being a nanoscale molecule, has recently been used in bottom-up nanotechnology with applications in sensing, nano-robotics, and computing. In this article, we propose a simple experiment involving the synthesis of a DNA nanoswitch that can change its shape from a linear "off" state to a looped "on" state in the presence of a target DNA molecule. The experiment also demonstrates the programmable topology of the looped state of the nanoswitch and its effect on gel migration. The experiment is easy to adapt in an undergraduate laboratory, requires only agarose gel electrophoresis, a minimal set-up cost for materials, and can be completed in a 3-hour time frame.

18.
Small ; 15(26): e1805386, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-30985074

RESUMO

Precise control of DNA base pairing has rapidly developed into a field full of diverse nanoscale structures and devices that are capable of automation, performing molecular analyses, mimicking enzymatic cascades, biosensing, and delivering drugs. This DNA-based platform has shown the potential of offering novel therapeutics and biomolecular analysis but will ultimately require clever modification to enrich or achieve the needed "properties" and make it whole. These modifications total what are categorized as the molecular hero suit of DNA nanotechnology. Like a hero, DNA nanostructures have the ability to put on a suit equipped with honing mechanisms, molecular flares, encapsulated cargoes, a protective body armor, and an evasive stealth mode.


Assuntos
DNA/química , Nanoestruturas/química , Nanotecnologia/métodos , Pareamento de Bases , Técnicas Biossensoriais/métodos , Conformação de Ácido Nucleico
19.
Langmuir ; 35(14): 5050-5053, 2019 04 09.
Artigo em Inglês | MEDLINE | ID: mdl-30879305

RESUMO

We report a triplex-based DNA device coupled with molybdenum disulfide (MoS2) nanosheets for use as a pH-sensing platform. The device transitions from a duplex state at pH 8 to a triplex state at pH 5. The interaction of the device with MoS2 nanosheets in the two states is read out as a fluorescence signal from a pH-insensitive dye attached to the device. We characterized the operation of the DNA device on MoS2 nanosheets, analyzed the pH response, and tested the reversibility of the system. Our strategy can lead to the creation of a suite of biosensors where the sensing element is a triplex DNA device and the signal response is modulated by inorganic nanomaterials.


Assuntos
DNA/química , Dissulfetos/química , Molibdênio/química , Nanoestruturas/química , Fluorescência , Concentração de Íons de Hidrogênio , Tamanho da Partícula , Propriedades de Superfície
20.
Nucleic Acids Res ; 45(19): 11459-11465, 2017 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-28977499

RESUMO

DNA serves as nature's information storage molecule, and has been the primary focus of engineered systems for biological computing and data storage. Here we combine recent efforts in DNA self-assembly and toehold-mediated strand displacement to develop a rewritable multi-bit DNA memory system. The system operates by encoding information in distinct and reversible conformations of a DNA nanoswitch and decoding by gel electrophoresis. We demonstrate a 5-bit system capable of writing, erasing, and rewriting binary representations of alphanumeric symbols, as well as compatibility with 'OR' and 'AND' logic operations. Our strategy is simple to implement, requiring only a single mixing step at room temperature for each operation and standard gel electrophoresis to read the data. We envision such systems could find use in covert product labeling and barcoding, as well as secure messaging and authentication when combined with previously developed encryption strategies. Ultimately, this type of memory has exciting potential in biomedical sciences as data storage can be coupled to sensing of biological molecules.


Assuntos
Computadores Moleculares , DNA Viral/química , Armazenamento e Recuperação da Informação/métodos , Nanoestruturas/química , Bacteriófago M13/genética , DNA Viral/genética , Eletroforese em Gel de Ágar , Reprodutibilidade dos Testes
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