The impact of nanomaterials on autophagy across health and disease conditions.

Autophagy, a catabolic process integral to cellular homeostasis, is constitutively active under physiological and stress conditions. The role of autophagy as a cellular defense response becomes particularly evident upon exposure to nanomaterials (NMs), especially environmental nanoparticles (NPs) and nanoplastics (nPs). This has positioned autophagy modulation at the forefront of nanotechnology-based therapeutic interventions. While NMs can exploit autophagy to enhance therapeutic outcomes, they can also trigger it as a pro-survival response against NP-induced toxicity. Conversely, a heightened autophagy response may also lead to regulated cell death (RCD), in particular autophagic cell death, upon NP exposure. Thus, the relationship between NMs and autophagy exhibits a dual nature with therapeutic and environmental interventions. Recognizing and decoding these intricate patterns are essential for pioneering next-generation autophagy-regulating NMs. This review delves into the present-day therapeutic potential of autophagy-modulating NMs, shedding light on their status in clinical trials, intervention of autophagy in the therapeutic applications of NMs, discusses the potency of autophagy for application as early indicator of NM toxicity.

Micro-nanoemulsion and nanoparticle-assisted drug delivery against drug-resistant tuberculosis: recent developments.

Tuberculosis (TB) is a major global health problem and the second most prevalent infectious killer after COVID-19. It is caused by Mycobacterium tuberculosis (Mtb) and has become increasingly challenging to treat due to drug resistance. The World Health Organization declared TB a global health emergency in 1993. Drug resistance in TB is driven by mutations in the bacterial genome that can be influenced by prolonged drug exposure and poor patient adherence. The development of drug-resistant forms of TB, such as multidrug resistant, extensively drug resistant, and totally drug resistant, poses significant therapeutic challenges. Researchers are exploring new drugs and novel drug delivery systems, such as nanotechnology-based therapies, to combat drug resistance. Nanodrug delivery offers targeted and precise drug delivery, improves treatment efficacy, and reduces adverse effects. Along with nanoscale drug delivery, a new generation of antibiotics with potent therapeutic efficacy, drug repurposing, and new treatment regimens (combinations) that can tackle the problem of drug resistance in a shorter duration could be promising therapies in clinical settings. However, the clinical translation of nanomedicines faces challenges such as safety, large-scale production, regulatory frameworks, and intellectual property issues. In this review, we present the current status, most recent findings, challenges, and limiting barriers to the use of emulsions and nanoparticles against drug-resistant TB.

Microplastic interactions in the agroecosystems: methodological advances and limitations in quantifying microplastics from agricultural soil.

The instantaneous growth of the world population is intensifying the pressure on the agricultural sector. On the other hand, the critical climate changes and increasing load of pollutants in the soil are imposing formidable challenges on agroecosystems, affecting productivity and quality of the crops. Microplastics are among the most prevalent pollutants that have already invaded all terrestrial and aquatic zones. The increasing microplastic concentration in soil critically impacts crop plants growth and yield. The current review elaborates on the behaviors of microplastics in soil and their impact on soil quality and plant growth. The study shows that microplastics alter the soil's biophysical properties, including water-holding capacity, bulk density, aeration, texture, and microbial composition. In addition, microplastics interact with multiple pollutants, such as polyaromatic hydrocarbons and heavy metals, making them more bioavailable to crop plants. The study also provides a detailed insight into the current techniques available for the isolation and identification of soil microplastics, providing solutions to some of the critical challenges faced and highlighting the research gaps. In our study, we have taken a holistic, comprehensive approach by analysing and comparing various interconnected aspects to provide a deeper understanding of all research perspectives on microplastics in agroecosystems.

Antibacterial activity of a novel compound isolated from Bacillus licheniformis for treating bacterial infections in fishes: An in-silico approach.

Aeromonas hydrophila is a fish pathogen which is widely associated with diseases related to freshwater fishes. Vibrio parahemolyticus is a major globally emerging marine pathogen. Seven novel compounds were extracted from the ethyl acetate extract of Bacillus licheniformis, a novel marine bacterium isolated from marine actinomycetes. The compounds were identified using Gas Chromatography-Mass Spectroscopy (GC-MS). Only one bioactive compound having potent antibacterial activity was virtually screened to understand its drug-like property according to Lipinski's rule. The core proteins, 3L6E and 3RYL from the pathogens, A. hydrophila and V. parahemolyticus were targeted for drug discovery. In the present in-silico approach, Phenol,2,4-Bis(1,1-Dimethylethyl) a potent bioactive compound present in Bacillus licheniformis was used to prevent the infection due to the two pathogens. Further, using this bioactive compound, molecular docking was done to block their specific target proteins. This bioactive compound satisfied all the five rules of Lipinski. Molecular docking result revealed the best binding efficacy of Phenol,2,4-Bis(1,1-Dimethylethyl) against 3L6E and 3RYL with - 4.24 kcal/mol and - 4.82 kcal/mol, respectively. Molecular dynamics (MD) simulations were also executed to determine the binding modes as well as the stability of the protein-ligand docking complexes in the dynamic structure. The in vitro toxicity analysis of this potent bioactive compound against Artemia salina was carried out, revealing the non-toxic nature of B. licheniformis ethyl acetate extract. Thus, the bioactive compound of B. licheniformis was found to be a potent antibacterial agent against A. hydrophila and V. parahemolyticus.

Molecular Interaction of Functionalized Nanoplastics with Human Hemoglobin.

Humans are exposed to excessive nanoplastics (NPs) which have ample affinity for globular proteins. We investigated the interaction of functionalized polystyrene nanoplastics (plain: PS, carboxy: PS-COOH, and amine: PS-NH2) with human hemoglobin (Hb) utilizing multi-spectroscopic and docking approaches to acquire insights into molecular aspects of binding mechanism, which will be helpful in assessing the toxicokinetics or toxicodynamics of nanoplastics NPs. Hypsochromicity and hypochromicity were observed invariably in all the spectra (steady-state fluorescence emission, synchronous and three-dimensional) for all complexes, among which PS-NH2 binds effectively and changes the Hb's conformation by enhancing hydrophobicity around aromatic residues, notably tryptophan. All the NPs bind with the hydrophobic pocket of B-chain in Hb, where PS and PS-NH2 bind via hydrophobic force while PS-COOH binds via hydrogen bonding (predominantly) and van der Waals force, consistent validated with docking results. The minimal shift in absorbance peak also indicates enhanced hydrophobicity by PS-NH2 with larger aggregation as demonstrated in resonance light scattering. The amide band's shift, secondary structural analysis, and presence of characteristic functional group peaks in complexes in Infra-Red spectra confirm the structural changes in the protein. As seen in field emission scanning microscopy images, NPs penetrate the surface of proteins. These findings conclude that polystyrene NPs interact with Hb, causing structural alterations that may affect functional characteristics as well, with the greatest effect being in the order: PS-NH2>PS-COOH>PS.

Prepared microplastics interaction with Artemia salina under low pH conditions representing ocean acidification; a simulated environmental exposure.

Ocean acidification and microplastic pollution are two of the major ecological concerns. The distribution of large quantities of plastic debris and microplastics all across the oceans emphasises the need to determine the influence of microplastics in ocean acidification and to evaluate its concomitant toxicological effects on aquatic life forms. Studies on the combined impact of both the stressors are very limited, but much needed in the current scenario. Where most of the present-day research use purchased microplastics of defined size and morphology (microspheres, fibres, rods, etc.), the present study employs prepared "true to life microplastics" that resemble the environmental microplastic pollutants in morphology and size heterogeneity. The present study focusses on evaluating the fate and impact of oceanic microplastics on the physiology and development of Artemia salina (Brine shrimp), one among the most ecologically significant zooplankton species. Natural sea water was acidified by controlled perturbation of carbon dioxide using a valve system. The hatching rate of A. salina cysts receded significantly (p < 0.05) upon singular exposures to microplastics and low pH (7.80), whereas combined effect was insignificant. The reactive oxygen species (ROS) elevated as a result of individual exposures to microplastics and low pH. However, only in 0.5 mg mL-1 PE treatments at pH 7.80, an additive impact was reported for ROS activity (p < 0.05). The SOD activities increased significantly but it can be attributed as the individual responses towards exposure to both the stressors. A significant additive impact was not observed for SOD activity (p > 0.05). But during the development, significant morphological anomalies were observed. Changes in the appendages of nauplii and juveniles as a result of combined exposure to microplastics and low pH treatments are significant findings. Our observations suggest that coupled exposure to microplastics and low pH could induce significant oxidative stress in the marine zooplanktons and also adversely affect their normal development. Findings from the current study emphasise the need for further research to understand the coupled toxicological impacts of ocean acidification and predominant pollutants such as microplastics to other marine animals as well.

Multispectroscopy analysis of polystyrene nanoplastic interaction with diastase α-amylase.

The digestive enzyme of plant are generally α-amylase. They functions enzyme that breakdown starch into maltose and sugars. This happens in the endosperm of the seed. Due to pollutants, this process get happened one of emergent xenobiotics are micro and nano plastics. This study involves the interaction 100 nm size of polystyrene nano plastic (PSNPs) on α-amylase. The hyperchromism of α-amylase - PSNPs conjugate's revealed that ground-state complex in a microenvironment. Fluorescence quenching happened when the concentration of PSNPs was increased. The Stern Volmer plot revealed binding constant (Ka) was 1.904 × 1019 M-1. S-1 while the quenching constant (Kq) was 1.036 × 1011 M-1, the blue shift of the peak showed static quenching. The binding constant was KA = 4.2 × 1012, the number of binding site on PSNPs for α-amylase was n = 1.12. The synchronous result showed a gradual reduction in the intensity of Trp residues because when the α-amylase interacts with PSNPs short-range π-π interaction happens around the Trp163 residues. The enzyme activity of α-amylase by 44 % and its IC50 value was found to be 100 µg/mL. The enzyme kinetics (Vmax) analysis showed the type of inhibition with and without PSNPs Vmax 769 and Vmax 303 µg/mL/min, uncompetitive inhibition respectively. The effect of PSNPs on the enzymatic activity of α-amylase showed structural alterations of the protein. Therefore the in vitro and in silico studies were shown evidence of interaction between α-amylase and PSNPs leads to conformational structural changes in α-amylase.

Exposure to polystyrene nanoplastics impairs lipid metabolism in human and murine macrophages in vitro.

The use of polystyrene micro and nanoplastics in cosmetics and personal care products continues to grow every day. The harmful effects of their biological accumulation in organisms of all trophic levels including humans have been reported by several studies. While we have accumulating evidence on the impact of nanoplastics on different organ systems in humans, only a handful of reports on the impact of polystyrene nanoplastics upon direct contact with the immune system at the cellular level are avialable. The present study offers significant evidence on the cell-specific harmful impact of sulfate-modified nanoplastics (S-NPs) on human macrophages. Here we report that exposure of human macrophages to S-NPs (100 µg/mL) stimulated the accumulation of lipids droplets (LDs) in the cytoplasm resulting in the differentiation of macrophages into foam cells. The observed effect was specific for human and murine macrophages but not for other cell types, especially human keratinocytes, liver, and lung cell models. Furthermore, we found that S-NPs mediated LDs accumulation in human macrophages was accompanied by acute mitochondrial oxidative stress. The accumulated LDs were further delivered and accumulated into lysosomes leading to impaired lysosomal clearance. In conclusion, our study reveals that exposure to polystyrene nanoplastics stabilized with anionic surfactants can be a potent stimulus for dysregulation of lipid metabolism and macrophage foam cell formation, a characteristic feature observed during atherosclerosis posing a serious threat to human health.

Nanoemulsions: The rising star of antiviral therapeutics and nanodelivery system-current status and prospects.

Nanoemulsions (NEs) of essential oil (EO) have significant potential to target microorganisms, especially viruses. They act as a vehicle for delivering antiviral drugs and vaccines. Narrowing of drug discovery pipeline and the emergence of new viral diseases, especially, coronavirus disease, have created a niche to use NEs for augmenting currently available therapeutic options. Published literature demonstrated that EOs have an inherent broad spectrum of activity across bacterial, fungal, and viral pathogens. The emulsification process significantly improved the efficacy of the active ingredients in the EOs. This article highlights the research findings and patent developments in the last 2 years especially, in EO antiviral activity, antiviral drug delivery, vaccine delivery, viral resistance development, and repurposing EO compounds against SARS-CoV-2.

Prospects on the nano-plastic particles internalization and induction of cellular response in human keratinocytes.

BACKGROUND: Today, cosmetic products are very popular with both men and women to improve their appearance and increase their social acceptability. RESULTS: In this study, nano-sized (30-300 nm) plastic particles were isolated from the commercial face-scrubs and treated on the human keratinocytes. The observed adherence of polyethylene nano-plastics (PENPs), polystyrene NPs (PSNPs), and face-scrubs isolated nano-plastics (NPs) on the keratin layer reveals a significant attachment of NPs from the cosmetics that are applied on the skin for a short duration. This attachment property could facilitate further adherence of protein molecules on NPs and the protein-corona formation. The protein-corona mimics protein aggregates, thereby triggers macropinocytosis, followed by the macropinolysosomal process in the cell. These internalized NPs induced the concentration-dependent cytotoxic, cytostatic and cytoprotective activity in keratinocytes. Both single dose and chronic long-term exposure of lethal and sub-lethal concentrations of NPs resulted in oxidative stress-mediated down-regulation of cell growth and proliferation inhibition. Autophagic structures and premature aging were also observed using an electron microscopy and a senescence marker, respectively in the NPs internalized HaCaT cells incubated in a fresh, NPs-free medium. CONCLUSION: Though 2D culture models have many limitations, it produces significant conceptual advancements. This work provides an insight into the NPs concentration-dependent regulatory, cytoprotective, and cytotoxic effects in HaCaT cells. However, 3D model studies are required to identify the detailed mechanisms of NPs toxicity and cytoprotective events in cells at the molecular level.

Enhanced mosquitocidal efficacy of colloidal dispersion of pyrethroid nanometric emulsion with benignity towards non-target species.

The rising threat of vector-borne diseases and environmental pollution has instigated the investigation of nanotechnology-based applications. The current study deals with a nanotechnological application involving the usage of nanometric pesticides such as permethrin nanoemulsion. The mean droplet diameter and zeta potential of the prepared permethrin nanoemulsion were found to be 12.4 ±â€¯1.13 nm and -20.4 ±â€¯0.56 mV, respectively. The temporal stability of permethrin nanoemulsion was found to be 4 days when checked in the external environment. The permethrin nanoemulsion exhibited LC50 values of 0.038 and 0.047 mgL-1 and 0.049 and 0.063 mgL-1 against larval and pupal stages of Culex tritaeniorhynchus and Aedes aegypti, respectively. The results obtained from the larvicidal and pupicidal assay were corroborated with the histopathological and biochemical profiles of hosts upon treatment with nanometric pesticide. Further, the biosafety studies of the nanopesticide were carried out against different non-target species like freshwater algae (Closterium), Cicer arietinum (Chickpea) and Danio rerio (Zebrafish), and the mosquitocidal concentration of nanopesticide was found to be non-toxic. The following study, therefore, describes the mosquitocidal efficacy of nanometric pesticide formulated in a greener approach, which can become a substitute for conventional pesticide application in an eco-benign manner.

Effect of microencapsulated probiotic Bacillus vireti 01-polysaccharide extract of Gracilaria folifera with alginate-chitosan on immunity, antioxidant activity and disease resistance of Macrobrachium rosenbergii against Aeromonas hydrophila infection.

Polysaccharide from red seaweed Gracilaria folifera has an interesting functional property of antioxidant activity and prebiotic effect. A feeding trial experiment was directed to examine the effect of probiotic bacteria Bacillus vireti 01 microencapsulated with G. folifera polysaccharide against freshwater prawn M. rosenbergii. Three different feeding trials were conducted for 15 days. The first group contained prawns fed with commercial diet. The second group was comprised of Aeromonas hydrophila challenged prawns fed with commercial feed. The third group consisted of A. hydrophila challenged prawns fed with microencapsulated probiotic-polysaccharide. Survival percentage was significantly decreased in prawns of group2 as compared to that of group1 and group3 prawns (p < 0.0001). The immunological parameters and antioxidant activities (p < 0.001) were found to be increased in group three prawns which were fed with encapsulated probiotic-seaweed polysaccharide and challenged with A. hydrophila as compared to that of group1 and group2. Tissue necrosis, fused lamella, haemocyte infiltration and damage of hepatopancreas lumen and tubule were noted in group2 prawns. There was no histological changes were observed in group3 prawns in which the histological architecture was similar to the control group1. The results suggested that combination of encapsulated probiotic B. vireti 01 and seaweed polysaccharide as dietary feed showed an enhancement of immune response, antioxidant activity and disease resistant of M. rosenbergii against A. hydrophila.

Toxicity assessment of zero valent iron nanoparticles on Artemia salina.

The present study deals with the toxicity assessment of two differently synthesized zero valent iron nanoparticles (nZVI, chemical and biological) as well as Fe2+ ions on Artemia salina at three different initial concentrations of 1, 10, and 100 mg/L of these particles. The assessment was done till 96 h at time intervals of 24 h. EC50 value was calculated to evaluate the 50% mortality of Artemia salina at all exposure time durations. Between chemically and biologically synthesized nZVI nanoparticles, insignificant differences in the level of mortality were demonstrated. At even 24 h, Fe2+ ion imparted complete lethality at the highest exposure concentration (100 mg/L). To understand intracellular oxidative stress because of zero valent iron nanoparticles, ROS estimation, SOD activity, GSH activity, and catalase activity was performed which demonstrated that ionic form of iron is quite lethal at high concentrations as compared with the same concentration of nZVI exposure. Lower concentrations of nZVI were more toxic as compared with the ionic form and was in order of CS-nZVI > BS-nZVI > Fe2+ . Cell membrane damage and bio-uptake of nanoparticles were also evaluated for all three concentrations of BS-nZVI, CS-nZVI, and Fe2+ using adult Artemia salina in marine water; both of which supported the observations made in toxicity assessment. This study can be further explored to exploit Artemia salina as a model organism and a biomarker in an nZVI prone aquatic system to detect toxic levels of these nanoparticles. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 1617-1627, 2017.

The Environmentally Benign form of Pesticide in Hydrodispersive Nanometric form with Improved Efficacy Against Adult Mosquitoes at Low Exposure Concentrations.

Permethrin, a poorly water-soluble synthetic pesticide belonging to the pyrethroid family, was formulated into water-dispersive nanometric form by rapid evaporation of pesticide loaded oil-in-water microemulsion. The mean hydrodynamic diameter of Nanopermethrin was found to be 199.01 ± 1.4 nm. The efficacy of the Nanopermethrin was comparatively investigated with its bulk form against 2-3 days old adult mosquitoes by WHO cone bioassay for 60 min. The median knockdown concentration of Culex tritaeniorhynchus, Culex quinquefasciatus and Aedes albopictus were found to be 7.20 × 10(4), 7.53 × 10(4), 0.42 × 10(3) mg/L for Bulk permethrin, and 0.98 × 10(4), 1.17 × 10(4), 0.05 × 10(3) mg/L for Nanopermethrin, respectively. The obtained results extrapolate the improved efficacy of Nanopermethrin even at low-level concentrations. Hence, the formulated Nanopermethrin will serve as an effective alternative pesticide in controlling the mosquito population with reduced environmental toxicity.

Cytogenetic studies of chromium (III) oxide nanoparticles on Allium cepa root tip cells.

The current study evaluates the cytogenetic effects of chromium (III) oxide nanoparticles on the root cells of Allium cepa. The root tip cells of A. cepa were treated with the aqueous dispersions of Cr2O3 nanoparticles (NPs) at five different concentrations (0.01, 0.1, 1, 10, and 100µg/mL) for 4hr. The colloidal stability of the nanoparticle suspensions during the exposure period were ascertained by particle size analyses. After 4hr exposure to Cr2O3 NPs, a significant decrease in mitotic index (MI) from 35.56% (Control) to 35.26% (0.01µg/mL), 34.64% (0.1µg/mL), 32.73% (1µg/mL), 29.6% (10µg/mL) and 20.92% (100µg/mL) was noted. The optical, fluorescence and confocal laser scanning microscopic analyses demonstrated specific chromosomal aberrations such as-chromosome stickiness, chromosome breaks, laggard chromosome, clumped chromosome, multipolar phases, nuclear notch, and nuclear bud at different exposure concentrations. The concentration-dependent internalization/bio-uptake of Cr2O3 NPs may have contributed to the enhanced production of anti oxidant enzyme, superoxide dismutase to counteract the oxidative stress, which in turn resulted in observed chromosomal aberrations and cytogenetic effects. These results suggest that A. cepa root tip assay can be successfully applied for evaluating environmental risk of Cr2O3 NPs over a wide range of concentrations.

Cytotoxicity of TiO2 nanoparticles towards freshwater sediment microorganisms at low exposure concentrations.

There is a persistent need to assess the effects of TiO2 nanoparticles on the aquatic ecosystem owing to their increasing usage in consumer products and risk of environmental release. The current study is focused on TiO2 nanoparticle-induced acute toxicity at sub-ppm level (≤1ppm) on the three different freshwater sediment bacterial isolates and their consortium under two different irradiation (visible light and dark) conditions. The consortium of the bacterial isolates was found to be less affected by the exposure to the nanoparticles compared to the individual cells. The oxidative stress contributed considerably towards the cytotoxicity under both light and dark conditions. A statistically significant increase in membrane permeability was noted under the dark conditions as compared to the light conditions. The optical and fluorescence microscopic images showed aggregation and chain formation of the bacterial cells, when exposed to the nanoparticles. The electron microscopic (SEM, TEM) observations suggested considerable damage of cells and bio-uptake of nanoparticles. The exopolysaccrides (EPS) production and biofilm formation were noted to increase in the presence of the nanoparticles, and expression of the key genes involved in biofilm formation was studied by RT-PCR.

Autocatalytic growth of biofunctionalized antibacterial silver nanoparticles.

Development of eco-friendly processes for nanosynthesis is gaining importance owing to the widespread application of nanoparticles (NPs). In the present study, we have explained the mechanism and kinetics of bioreduction in the biosynthesis of silver nanoparticles (AgNPs) using aqueous leaf extract of Rhizophora apiculata. Spectrophotometric methods were employed to quantify the bioactive molecules present before and after the reduction process. The results showed that the polyphenols were the main components responsible for the biosynthesis of AgNPs, which was further confirmed by Fourier transform infrared spectroscopy. The kinetics of formation of AgNPs were monitored by time-resolved spectrophotometric and X-ray diffraction studies, which revealed that the NP formation is an autocatalytic process with a rate constant of 1.9 × 10(-2)  Min(-1) . The NPs were characterized using spectroscopic and microscopic techniques like ultraviolet-visible absorption spectroscopy, dynamic light scattering, transmission electron microscopy, scanning electron microscopy-energy-dispersive X-ray spectroscopy, and X-ray diffraction. The biogenic AgNPs showed substantial inhibitory activity to Proteus mirabilis, Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus with minimum inhibitory concentration values of 2.5, 12.5, 2.5, and 31.25 µg/mL, respectively. The current research provides an insight into the mechanistic aspects of bioreduction and formation of AgNPs.

Study on antimicrobial potential of neem oil nanoemulsion against Pseudomonas aeruginosa infection in Labeo rohita.

Presence of several biochemical constituents in neem makes it an efficient antimicrobial agent for pathogenic diseases. The current investigation was aimed to assess the therapeutic potential of neem nanoemulsion as a control measure for Pseudomonas aeruginosa infection in freshwater fish Labeo rohita. The median lethal concentration (LC50) for the neem oil and neem nanoemulsion was 73.9 and 160.3 mg/L, respectively. The biomarker enzymes of treated fish tissues showed a significant difference in the level of glutathione reductase, catalase, and lipid peroxidation in neem oil-treated samples than in neem nanoemulsion-treated samples at P<0.05. The results were corroborative with histopathology and ultrastructural analysis. The bacterial infection of P. aeruginosa treated using neem nanoemulsion was more effective in both in vitro and in vivo methods. Present findings suggest that neem-based nanoemulsion has negligible toxicity to Rohu fishes. This makes neem-based nanoemulsion as an efficient therapeutic agent against P. aeruginosa infection, leading to its possible usage in the aquaculture industry.

Cancerous cell targeting and destruction using pH stabilized amperometric bioconjugated gold nanoparticles from marine macroalgae, Padina gymnospora.

The main aim of this study was, using biomechanistic approach, to synthesize and characterize amperometric stable gold nanoparticles (AuNPs) under different pH conditions using UV Spec, dynamic light scattering and TEM with energy dispersive X-ray analysis. The biomolecules involved in conjugation and reduction were further characterized by Fourier transform infrared analysis. The pH stabilized nanoparticles were studied to determine the functional and molecular mechanism of cell death on liver cancer (HepG2) cell line and gastric cancer (YCC3) cell line. The zeta potential and TEM imaging demonstrated that AuNPs were spherical in nature and can pass through the cellular membrane because of their intrinsic properties of AuNPs to bind to carbon-bonded sulfhydryl (-C-SH or R-SH) group and, therefore, could interact with intracellular components of the cell which was confirmed through phase contrast microscopy. Altered molecular mechanism and cellular effects in different cancer cell suggest a potential for in vivo applications of gold nanomaterials.

A combined toxicological impact on Artemia salina caused by the presence of dust particles, microplastics from cosmetics, and paracetamol.

Environmental pollution poses a significant and pressing threat to the overall well-being of aquatic ecosystems in modern society. This study showed that pollutants like dusts from AC filter, fan wings and Traffic dust PM 2.5 were exposed to Artemia salina in pristine form and in combination. The findings indicated that exposure to multi-pollutants had a detrimental effect on the hatching rates of A. salina cysts. Compared to untreated A. salina, the morphology of adult (7th day old) A. salina changed noticeably after each incubation period (24-120 h). Oxidative stress increased considerably as the exposure duration increased from 24 to 120 h compared to the control group. There was a time-dependent decline in antioxidant enzyme activity and total protein concentration. When all particles were used all together, the total protein content in A. salina decreased significantly. All particles showed a considerable decline in survival rate. Those exposed to traffic dust particles showed significantly higher levels of oxidative stress and antioxidant activity than those exposed to other particles.