Air-Coupled Ultrasonic Probe Integrity Test Using a Focused Transducer with Similar Frequency and Limited Aperture for Contrast Enhancement.

Air-coupled ultrasonic probes require a special design approach and handling due to the significant mismatch to the air. Outer matching layers have to be soft so can be easily damaged and excitation voltages might cause the degradation of electrodes or bonding between the layers. Integrity inspection is desired during design, manufacturing, and exploitation. Spatial distribution of a transduction efficiency over piezoelement surface is proposed as a convenient means for the air-coupled probe integrity inspection. Focused transducer of similar center frequency is used to scan the surface of the inspected probe. However, such approach creates a challenge, i.e., area of the scanning beam is much smaller than the total receiving area of the inspected probe, therefore, contrast and imaging resolution are significantly degraded. Masking aperture made from cardboard and felt, placed at the focal point was proposed as solution. Far-range sidelobes were suppressed down to the noise floor (-50 dB) and the near-range sidelobes were reduced down to -17 dB. The proposed modification allows to use a similar frequency focused transducer. Probe integrity inspection can be carried out at significantly enhanced contrast and lateral resolution. Natural and artificial defects can be detected by the use of the proposed method.

The Assessment of Calcium and Bleomycin Cytotoxic Efficiency in Relation to Cavitation Dosimetry.

Microbubble (MB)- and ultrasound (US)-facilitated intracellular Ca2+ delivery, known as sonoporation (SP), is a promising anticancer treatment modality, since it allows a spatio-temporally controllable and side-effect-free alternative to conventional chemotherapy. The current study provides extensive evidence that a 5 mM concentration of Ca2+ in combination with US alone or US and Sonovue MBs can be an alternative to the conventional 20 nM concentration of the anticancer drug bleomycin (BLM). Ca2+ application together with SP induces a similar level of death in Chinese hamster ovary cells to the combination of BLM and SP but does not cause systemic toxicity, as is inherent to conventional anticancer drugs. In addition, Ca2+ delivery via SP alters three vital characteristics essential for viable cells: membrane permeability, metabolic activity and proliferation ability. Most importantly, Ca2+ delivery via SP elicits sudden cell death-occurring within 15 min-which remains similar during 24-72 h and 6 d periods. The extensive study of US waves side-scattered by MBs led to the quantification of the cavitation dose (CD) separately for subharmonics, ultraharmonics, harmonics and broadband noise (up to 4 MHz). The CD was suitable for the prognostication of the cytotoxic efficiency of both anticancer agents, Ca2+ and BLM, as was indicated by an overall high (R2 ≥ 0.8) correlation (22 pairs in total). These extensive analytical data imply that a broad range of frequencies are applicable for the feedback-loop control of the process of US-mediated Ca2+ or BLM delivery, successively leading to the eventual standardization of the protocols for the sonotransfer of anticancer agents as well as the establishment of a universal cavitation dosimetry model.

Ultrasonic needle hydrophone calibration in air by a parabolic off-axis mirror focused beam using three-transducer reciprocity.

An acoustic field distribution investigation in air requires a small receiving sensor. Needle hydrophones seem to be an attractive solution, and it has previously been demonstrated that needle hydrophones designed for use in water can be used in air. The metrology problem is that an absolute sensitivity calibration is needed, because needle hydrophones are not characterized in air, especially for frequencies below 1 MHz, which is of interest for air-coupled ultrasound. Conventional, three-transducer/microphone reciprocity calibration requires measurements to be done in the far field. However, when transducer diameter is large and the frequency is high, the required measurement distance becomes very large: 3 m for a 20 mm source, transmitting at 1 MHz. Large propagation distance leads to high attenuation and nonlinear effects in air propagation, and distortion and losses accumulate. Small needle hydrophones have low sensitivity, so that high excitation amplitudes would be required, which can lead to transducer heating and increase nonlinearity effects. A derivative of the three-transducer reciprocity calibration method is proposed, where a large aperture transducer is focused onto a hydrophone, using hybrid of plane wave and spherical wave reciprocity. Use of a focused source minimizes the frequency-dependent diffraction effects, and the spherical wave approximation is valid at the focal distance, and low level excitation signals can be used. Focusing is accomplished using a parabolic off-axis mirror. Calibration is in transmission, which reduces the complexity of the electrical measurements. The corresponding equations have been derived for this setup. Calibration of the transducer and needle hydrophone absolute sensitivity is obtained.

Miniature Ferroelectret Microphone Design and Performance Evaluation Using Laser Excitation.

Miniature microphones suitable for measurements of ultrasonic wave field scans in air are expensive or lack sensitivity or do not cover the range beyond 100 kHz. It is essential that they are too large for such fields measurements. The use of a ferroelectret (FE) film is proposed to construct a miniature, needle-style 0.5-mm-diameter sensitive element ultrasonic microphone. FE has an acoustic impedance much closer to that of air compared with other alternatives and is low cost and easy to process. The performance of the microphone was evaluated by measuring the sensitivity area map, directivity, ac response, and calibrating the absolute sensitivity. Another novel contribution here is that the sensitivity map was obtained by scanning the focused beam of a laser diode over the microphone surface, producing thermoelastic ultrasound excitation. The electroacoustic response of the microphone served as a sensitivity indicator at a scan spot. Micrometer scale granularity of the FE sensitivity was revealed in the sensitivity map images. It was also demonstrated that the relative ac response of the microphone can be obtained using pulsed laser beam thermoelastic excitation of the whole microphone surface with a laser diode. The absolute sensitivity calibration was done using the hybrid three-transducer reciprocity technique. A large aperture, air coupled transducer beam was focused onto the microphone surface, using the parabolic off-axis mirror. This measurement validated the laser ac response measurements. The FE microphone performance was compared with biaxially stretched polyvinylidene difluoride (PVDF) microphone of the same construction.

Half bridge topology 500 V pulser for ultrasonic transducer excitation.

Application of half bridge topology for ultrasonic transducer excitation using long pulse trains is presented. The novelty of the approach is the high speed solution for a high side drive. A commercially available high speed digital isolator and a high speed MOSFET driver were combined to give the possibility to deliver fast driving signals to a high side N-channel MOSFET. The experimental investigation indicates that the output amplitude of the fundamental harmonic can reach 624 Vp-p for light loads and 552 Vp-p when driving 50 Ω loads. The operation frequency at such voltages can reach 10 MHz for unloaded or 50 Ω load condition and 6 MHz when driving capacitive 3000 pF loads. The output impedance is 13 Ω for voltages below 500 Vp-p and 16-26 Ω for voltages 500 Vp-p and above.

Efficient high voltage pulser for piezoelectric air coupled transducer.

The design of high voltage pulser for air coupled ultrasound imaging is presented. It is dedicated for air-coupled ultrasound applications when piezoelectric transducer design is used. Two identical N-channel MOSFETs are used together with 1200V high and low side driver IC. Simple driving pulses' delay and skew circuit is used to reduce the cross-conduction. Analysis of switch peak current and channel resistance relation to maximum operation frequency and load capacitance is given. PSPICE simulation was used to analyze the gate driver resistance, gate pulse skew, pulse amplitude influence on energy consumption when loaded by capacitive load. Experimental investigation was verified against simulation and theoretical predictions. For 500pF capacitance, which is most common for piezoelectric air coupled transducers, pulser consumes 650µJ at 1kV pulse and 4µJ at 50V. Pulser is capable to produce up to 1MHz pulse trains with positive 50V-1kV pulses with up to 10A peak output current. When loaded by 200kHz transducer at 1kV pulse amplitude rise time is 40ns and fall time is 32ns which fully satisfies desired 1MHz bandwidth.