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A surface enhanced Raman scattering (SERS) method is described for the determination of vanillin, methyl vanillin and ethyl vanillin at trace levels. Flower-like silver nanoparticles on a silicon wafer are used as the SERS substrate, and the analytes can be specifically and non-destructively recognized by their specific Raman bands. The molecules can be recognized rapidly by identifying the characteristic bands. The SERS spectra of vanillin (C8H8O3) were used as mid-contrast, and specific bands of methyl vanillin and ethyl vanillin (C9H10O3) were acquired at 775 cm-1, 1350 cm-1 and 1282 cm-1, 1382 cm-1, respectively. In addition, by using an improved principal component analysis (PCA) algorithm, the organic molecule can be quantitatively determined. Dissolved in water, vanillin, methyl vanillin and ethyl vanillin still can be detected at a concentration of 10-8 M, at which their characteristic Raman peaks are still visible. The method was successfully applied to the determination of vanillin in milk powder products. Graphical abstract Vanillin can be identified at trace levels by laser irradiation of milk and by using flower-like silver nanoparticles as the surface enhanced Raman scattering (SERS) substrate. Vanillin and its methyl and ethyl derivatives can be quantitatively analyzed by the principal component analysis (PCA) algorithm.
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MAIN CONCLUSION: Six types of lignin-carbohydrate complex (LCC) fractions were isolated from Eucalyptus. The acidic dioxane treatment applied significantly improved the yield of LCCs. The extraction conditions had a limited impact on the LCC structures and linkages. Characterization of the lignin-carbohydrate complex (LCC) structures and linkages promises to offer insight on plant cell wall chemistry. In this case, Eucalyptus LCCs were extracted by aqueous dioxane, and then precipitated sequentially by 70% ethanol, 100% ethanol, and acidic water (pH = 2). The composition and structure of the six LCC fractions obtained by selective precipitation were investigated by sugar analysis, molecular weight determination, and 2D HSQC NMR. It was found that the acidic (0.05-M HCl) dioxane treatment significantly improved the yield of LCCs (66.4% based on Klason lignin), which was higher than the neutral aqueous dioxane extraction, and the extraction condition showed limited impact on the LCC structures and linkages. In the fractionation process, the low-molecular-weight LCCs containing a high content of carbohydrates (60.3-63.2%) were first precipitated by 70% ethanol from the extractable solution. The phenyl glycoside (PhGlc) bonds (13.0-17.0 per 100Ar) and highly acetylated xylans were observed in the fractions recovered by the precipitation with 100% ethanol. On the other hand, such xylan-rich LCCs exhibited the highest frequency of ß-O-4 linkages. The benzyl ether (BE) bonds were only detected in the fractions obtained by acidic water precipitation.
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Carboidratos/isolamento & purificação , Eucalyptus/metabolismo , Lignina/isolamento & purificação , Metabolismo dos Carboidratos , Carboidratos/química , Precipitação Química , Dioxanos/uso terapêutico , Lignina/química , Lignina/metabolismo , Espectroscopia de Ressonância Magnética , Peso MolecularRESUMO
BACKGROUND: This study aims to investigate the outcomes of patients who received early fiberoptic bronchoscopic sputum aspiration and lavage after thoracoscopic and laparoscopic esophagectomy due to esophageal cancer. METHODS: A prospective randomized clinical trial was performed between March 2020 and June 2022. Patients who were scheduled for thoracoscopic and laparoscopic esophagectomy due to esophageal cancer were enrolled. Then, these patients were assigned to the control group (traditional postoperative care) and study group (traditional postoperative care with early bronchoscopic sputum aspiration and lavage). The outcomes, which included the length of hospital stay and medical expenses, and postoperative complications, which included pulmonary infection, atelectasis, respiratory dysfunction and anastomotic leakage, were compared between these two groups. RESULTS: A total of 106 patients were enrolled for the present study, and 53 patients were assigned for the control and study groups. There were no statistically significant differences in gender, age, and location of the esophageal cancer between the two groups. Furthermore, the length of hospital stay was statistically significantly shorter and the medical expenses were lower during hospitalization in the study group, when compared to the control group (12.3 ± 1.2 vs. 18.8 ± 1.3 days, 5.5 ± 0.9 vs. 7.2 ± 1.2 Chinese Yuan, respectively; all, P < 0.05). Moreover, there were statistically significantly fewer incidences of overall complications in study group, when compared to the control group (20.7% vs.45.2%, P < 0.05). CONCLUSIONS: For patients with esophageal cancer, early fiberoptic bronchoscopic sputum aspiration and lavage after thoracoscopic and laparoscopic esophagectomy can shorten the length of hospital stay, and lower the medical expense and incidence of postoperative complications.
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Neoplasias Esofágicas , Laparoscopia , Humanos , Irrigação Terapêutica/efeitos adversos , Escarro , Estudos Prospectivos , Complicações Pós-Operatórias/etiologia , Laparoscopia/efeitos adversos , Neoplasias Esofágicas/cirurgia , Neoplasias Esofágicas/complicações , Esofagectomia/efeitos adversos , Toracoscopia/efeitos adversos , Estudos RetrospectivosRESUMO
Botulinum neurotoxins (BoNTs) are potential biological weapons because of their high toxicity and mortality. Vaccination is an effective strategy to prevent botulism. The carboxyl-terminus of the heavy chain (Hc domain) is nontoxic and sufficient to generate protective immune responses against natural BoNTs in animals. To produce a vaccine suitable for human use, a recombinant non His-tagged isoform of the Hc domain of botulinum neurotoxin serotype B (BHc) was expressed in Escherichia coli and purified by sequential chromatography. The immunogenicity of recombinant E.coli-expressed BHc and the yeast-expressed mBHc antigens was explored and compared in Balb/c mice. BHc provided comparable protective potency but elicited significantly higher antibody titer and neutralization potency against BoNT/B after twice immunization, indicating that the recombinant BHc protein expressed in E.coli have better immunogenicity than the yeast-expressed mBHc. Moreover, a frequency and dose-dependent effect was observed in mice immunized with BHc subunit vaccine and the anti-BHc ELISA antibody titers correlated well with neutralizing antibody titers and protection potency. In summary, the Alhydrogel-formulated BHc subunit vaccine afforded effective protection against BoNT/B challenge. Therefore, the non-His-tagged and homogeneous BHc expressed in E.coli represents a good potential candidate subunit vaccine for human use.
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Anticorpos Antibacterianos/sangue , Anticorpos Neutralizantes/sangue , Vacinas Bacterianas/imunologia , Toxinas Botulínicas Tipo A/imunologia , Botulismo/prevenção & controle , Imunogenicidade da Vacina , Animais , Anticorpos Antibacterianos/imunologia , Anticorpos Neutralizantes/imunologia , Vacinas Bacterianas/administração & dosagem , Escherichia coli/genética , Feminino , Camundongos Endogâmicos BALB C , Sorogrupo , Vacinação , Vacinas de Subunidades Antigênicas/imunologia , Vacinas Sintéticas/administração & dosagem , Vacinas Sintéticas/imunologia , Leveduras/genéticaRESUMO
A better understanding of the lignin in the straw of rapeseed, Brassica campestris L., is a prerequisite for promoting the biorefinery industry of rapeseed. Two different methods for fractionating lignin from rapeseed straw were proposed in this study. Lignin in the raw material was isolated with alkaline solution and recovered by acid precipitation. A comparison between two lignin preparations obtained from two different methods has been made in terms of yield and purity. The structural features were investigated by gel permeation chromatography, FT-IR spectroscopy, 2D-HSQC NMR and 31P NMR. Taking into consideration of the yield and purity, the proposed methods are effective for extracting lignin. NMR results showed that syringyl (S) was the predominant unit over guaiacyl (G) or p-hydroxyphenyl (H) units in the lignin preparations, and linkages ß-O-4', ß-ß' and ß-5' were also identified and quantified by NMR techniques. This study demonstrated that the combination of hydrothermal or dilute-acid pretreatment and alkaline process could efficiently isolate the lignins from the rapeseed straw to further applications for industries. It was found that the enzymatic hydrolysis of the two-step pretreated rapeseed straw increased 5.9 times than the straw without treatment, which is benefit for bioethanol production from rapeseed straw.
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Brassica rapa/química , Fracionamento Químico/métodos , Etanol/química , Lignina/química , Ácidos/química , Álcalis/química , Biomassa , Hidrólise , Lignina/isolamento & purificação , Estrutura Molecular , Caules de Planta/química , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
Linear ultrasonics methods based on the principle of reflection, transmission, dissipation of sound waves have been traditionally used to detect delaminations in composite structures. However, when the delamination is in very early stages such that it is almost closed, or closed due to a compressive load, the linear methods may fail to detect such cases of delaminations. Nonlinear acoustics/ultrasonics have shown potential to identify damages in composite structures which are difficult to detect using conventional linear ultrasonic methods. The nonlinear method involves exciting the structure with a sinusoidal signal of certain (or multiple) frequency and observing the vibrations of the structure. The vibrations of the damage region differ significantly from intact regions and can be used to identify the damage. However due to the complex and varying nature of the nonlinear phenomena created by the interaction between the exciting signal and the damage, there are many variables at play which can lead to success or failure of the method. While experiments lead to the establishment of the method to be used as a damage detection technique, numerical simulations can help to explain the various phenomena associated with nonlinearity. This work presents a quick approach to model the nonlinear behavior caused by closed delaminations. The model is validated with a previously available approach for nonlinear vibrations modeling and a comparison is made between the two. The local nature of the nonlinearity enables to map out the area of damage in the structure. Additionally, a few parametric studies are performed to study the effect of various parameters related to the nonlinear phenomenon.
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The aim of the research was to evaluate the effect of combined treatments on fermentable sugar production from rapeseed straw. An optimum condition was found to be the combination of hydrothermal pretreatment at 180°C for 45min and post-treatment by 2% NaOH at 100°C for 2h, which was based on the quantity of monosaccharides released during enzymatic hydrolysis. As compared with the raw material without treatment, the combination of hydrothermal pretreatment and alkali post-treatment resulted in a significant increase of the saccharification rate by 5.9times. This process potentially turned rapeseed straw into value added products in accordance with the biorefinery concept.
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Biotecnologia/métodos , Brassica rapa/química , Fracionamento Químico/métodos , Biomassa , Celulases/química , Celulases/metabolismo , Celulose/química , Celulose/isolamento & purificação , Glucose/química , Hidrólise , Lignina/química , Lignina/isolamento & purificação , Brotos de Planta/química , Polissacarídeos/química , Polissacarídeos/isolamento & purificação , Hidróxido de Sódio/química , Temperatura , beta-Glucosidase/química , beta-Glucosidase/metabolismoRESUMO
As dendritic cells (DCs) play a critical role in priming antigen-specific immune responses, the efficacy of DNA vaccines may be enhanced by targeting the encoded antigen proteins to DCs. In this study, we constructed a DC-targeted DNA vaccine encoding the Hc domain of botulinum neurotoxin serotype A (AHc) fused with scDEC, a single-chain Fv antibody (scFv) specific for the DC-restricted antigen-uptake receptor DEC205. Intramuscular injections of mice with the DC-targeted DNA vaccine (pVAX1-scDEC-AHc) stimulated more DCs to mature than the non-targeted DNA vaccine (pVAX1-SAHc) in the splenocytes. The DC-targeted DNA vaccine could induce more DCs maturation at the site of inoculation. The DC-targeted DNA vaccine induced stronger AHc-specific humoral immune responses, lymphocyte proliferative responses and protective potency against BoNT/A in mice than did pVAX1-SAHc. Moreover, the DC-targeting DNA vaccine provided effective protection after only two inoculations. In summary, these results showed that the DC-targeted fusion DNA vaccine could generate strong immunity, indicating that maturation of DCs induced by pVAX1-scDEC-AHc may be helpful for priming and boosting immune responses. Thus, we propose that the strategy of targeting antigen to DCs in vivo via DEC205 can enhance effectively the potency of DNA vaccines against BoNTs or other pathogens in an animal model.