[Systematic review on efficacy and safety of Danshen Chuanxiongqin Injection in treatment of acute cerebral infarction].

To systemically evaluate the therapeutic efficacy and safety of Danshen Chuanxiongqin Injection in treatment of acute cerebral infarction and provide the reference of evidence-based medicine for its clinical safety and effective drug use. Databases including CNKI, WanFang Data, SinoMed, the Cochrane Library, EMbase and PubMed were searched from inception to April 2018 to collect the randomized controlled trials (RCTs) on Danshen Chuanxiongqin Injection in the treatment of acute cerebral infarction. The quality of all included studies was evaluated by two independent reviewers following the cochrane systematic review method and using Revman5.3 software and State13.0 for Meta-analysis. A total of 30 RCTs involving 3 233 patients with acute cerebral infarction were included in the study after literature quality evaluation. Meta-analysis showed that as compared with the control group of conventional western medicine alone, Danshen Chuanxiongqin Injection combined with conventional western medicine can achieve better efficacy in treatment of acute cerebral infarction, increase the clinical total effective rate (RR=1.22, 95% CI [1.18, 1.27], P<0.000 01) and activities of daily living (MD=9.42, 95% CI [8.12, 10.72], P<0.000 01), and improve the degree of neurological impairment (MD=-3.99, 95% CI [-4.89, -3.07], P<0.000 01). Furthermore, the result showed that Danshen Chuanxiongqin Injection in the treatment of acute cerebral infarction can significantly decrease the whole blood high-shear viscosity, whole blood low-shear viscosity, plasma viscosity, fibrinogen level and other hemorheological indexes (P<0.01). This Meta-analysis demonstrated that Danshen Chuan xiongqin injection in the treatment of acute cerebral infarction is safe and effective, but lacks the large multicenter clinical randomized trials to support the treatment outcome.

The Complete Mitochondrial Genome of Aleurocanthus camelliae: Insights into Gene Arrangement and Genome Organization within the Family Aleyrodidae.

There are numerous gene rearrangements and transfer RNA gene absences existing in mitochondrial (mt) genomes of Aleyrodidae species. To understand how mt genomes evolved in the family Aleyrodidae, we have sequenced the complete mt genome of Aleurocanthus camelliae and comparatively analyzed all reported whitefly mt genomes. The mt genome of A. camelliae is 15,188 bp long, and consists of 13 protein-coding genes, two rRNA genes, 21 tRNA genes and a putative control region (GenBank: KU761949). The tRNA gene, trnI, has not been observed in this genome. The mt genome has a unique gene order and shares most gene boundaries with Tetraleurodes acaciae. Nineteen of 21 tRNA genes have the conventional cloverleaf shaped secondary structure and two (trnS1 and trnS2) lack the dihydrouridine (DHU) arm. Using ARWEN and homologous sequence alignment, we have identified five tRNA genes and revised the annotation for three whitefly mt genomes. This result suggests that most absent genes exist in the genomes and have not been identified, due to be lack of technology and inference sequence. The phylogenetic relationships among 11 whiteflies and Drosophila melanogaster were inferred by maximum likelihood and Bayesian inference methods. Aleurocanthus camelliae and T. acaciae form a sister group, and all three Bemisia tabaci and two Bemisia afer strains gather together. These results are identical to the relationships inferred from gene order. We inferred that gene rearrangement plays an important role in the mt genome evolved from whiteflies.

Evolution of multipartite mitochondrial genomes in the booklice of the genus Liposcelis (Psocoptera).

BACKGROUND: The genus Liposcelis (Psocoptera: Troctomorpha) has more than 120 species with a worldwide distribution and they pose a risk for global food security. The organization of mitochondrial (mt) genomes varies between the two species of booklice investigated in the genus Liposcelis. Liposcelis decolor has its mt genes on a single chromosome, like most other insects; L. bostrychophila, however, has a multipartite mt genome with genes on two chromosomes. RESULTS: To understand how multipartite mt genome organization evolved in the genus Liposcelis, we sequenced the mt genomes of L. entomophila and L. paeta in this study. We found that these two species of booklice also have multipartite mt genomes, like L. bostrychophila, with the mt genes we identified on two chromosomes. Numerous pseudo mt genes and non-coding regions were found in the mt genomes of these two booklice, and account for 30% and 10% respectively of the entire length we sequenced. In L. bostrychophila, the mt genes are distributed approximately equally between the two chromosomes. In L. entomophila and L. paeta, however, one mt chromosome has most of the genes we identified whereas the other chromosome has largely pseudogenes and non-coding regions. L. entomophila and L. paeta differ substantially from each other and from L. bostrychophila in gene content and gene arrangement in their mt chromosomes. CONCLUSIONS: Our results indicate unusually fast evolution in mt genome organization in the booklice of the genus Liposcelis, and reveal different patterns of mt genome fragmentation among L. bostrychophila, L. entomophila and L. paeta.

Characterization of the complete mitochondrial genome of the tea slug moth, Iragoides fasciata (Lepidoptera: Limacodidae).

Moths of the family Limacodidae are major pests that damage tea trees, fruit trees, and forests. The complete mitochondrial genome of Iragoides fasciata (Lepidoptera: Limacodidae) was sequenced. The genome was found to be 15,645 bp in size (GenBank accession no. MK250437), including 13 protein-coding genes (PCGs), two ribosomal RNA genes (rRNAs), 22 transfer RNA genes (tRNAs), and a 431 bp A + T-rich region. Nucleotide composition showed a total A + T content of 82.03% with significant AT-bias. All PCGs were found to start with ATN codons and use the canonical stop codons TAA or incomplete T, except for cox1, which was found to utilize CGA as a start codon. Phylogenetic relationships were based on the 13 PCGs with 24 moths, showing that I. fasciata is more closely related to other slug moths in the family Limacodidae.

Effects of Anti-Diabetic Drugs on Fracture Risk: A Systematic Review and Network Meta-Analysis.

Purpose: Available data on the effects of anti-diabetic drugs on fracture risk are contradictory. Therefore, our study aimed to analyze all available data on the effects of anti-diabetic drugs on fracture risk in type 2 diabetes mellitus (T2DM) patients. Methods: Embase, Medline, ClinicalTrials.gov, and Cochrane CENTRAL were searched for relevant trials. All data analyses were performed with STATA (12.0) and R language (3.6.0). Risk ratio (RR) with its 95% confidence interval (CI) was calculated by combining data for the fracture effects of anti-diabetic drugs, including sodium-glucose co-transporter 2 (SGLT2) inhibitors, dipeptidyl peptidase-4 (DPP-4) inhibitors, glucagon-like peptide-1 (GLP-1) receptor agonists, meglitinides, α-glucosidase inhibitors, thiazolidinediones, biguanides, insulin, and sulfonylureas. Results: One hundred seventeen eligible randomized controlled trials (RCTs) with 221,364 participants were included in this study. Compared with placebo, trelagliptin (RR 3.51; 1.58-13.70) increased the risk of fracture, whereas albiglutide (RR 0.29; 0.04-0.93) and voglibose (RR 0.03; 0-0.11) decreased the risk of fracture. Other medications were comparable in terms of their effects on fracture risk, and no statistical significance was observed. In terms of fractures, voglibose (0.01%) may be the safest option, and trelagliptin (13.64%) may be the worst. Sensitivity analysis results were consistent with those of the main analysis. No statistically significant differences were observed in the regression coefficients of age (1.03; 0.32-2.1), follow-up duration (0.79; 0.27-1.64), and sex distribution (0.63; 0.15-1.56). Conclusions: We found varied results on the association between the use of anti-diabetic drugs and fracture risk. Specifically, trelagliptin raised the risk of fracture, whereas voglibose and albiglutide showed benefit with statistical difference. Other drugs were comparable in terms of their effects on fracture risk. Some drugs (omarigliptin, sitagliptin, vildagliptin, saxagliptin, empagliflozin, ertugliflozin, rosiglitazone, pioglitazone, and nateglinide) may increase the risk of fracture, while others (such as dulaglutide, exenatide, liraglutide, semaglutide, lixisenatide, linagliptin, alogliptin, canagliflozin, dapagliflozin, glipizide, gliclazide, glibenclamide, glimepiride, metformin, and insulin) may show benefits. The risk of fracture was independent of age, sex distribution, and the duration of exposure to anti-diabetic drugs. When developing individualized treatment strategies, the clinical efficacy of anti-diabetic drugs must be weighed against their benefits and risks brought about by individual differences of patients. Systematic Review Registration: This Systematic Review was prospectively registered on the PROSPERO (https://www.crd.york.ac.uk/PROSPERO/, registration number CRD42020189464).

The complete mitochondrial genome of the bagworm from a tea plantation in China, Eumeta variegata (Lepidoptera: Psychidae).

The mitochondrial (mt) genome of Eumeta variegata Snellen (Psychidae) has been sequenced and annotated. The mt genome has a total length of 15,793 bp, consisting of 13 protein-coding genes, 22 tRNA, two rRNA genes, and an AT-rich control region (GenBank accession no. MN242985). The nucleotide composition was extremely AT-rich, and the AT content is 81.57%. The gene order is consistent with other sequenced mt genome of moths and butterflies from Ditrysia. The sequence similarity of E. variegate mt genomes between the specimen of China and South Korea is 98.38%, whereas the similarity between the specimen of China and Japan is 90.61%. The sequence of PCGs and rRNAs among different specimens are similar, and many differences are detected at the region of A + T-rich region and the tRNA block 'ARNS1EF'.

The complete mitochondrial genome of the tea weevil, Myllocerinus aurolineatus (Coleoptera: Curculionidae).

The tea weevil, Myllocerinus aurolineatus (Voss), is a serious pest of tea plants. We have obtained and annotated the complete mitochondrial genome of M. aurolineatus (GenBank accession No. MH197100). The entire mt genome is 17,762 bp long with an A + T content of 75.45%. The mt genome of M. aurolineatus encodes all 37 genes that are typically found in animal mt genomes, consists of 13 protein-coding genes, 2 ribosomal RNA and 22 transfer RNA genes. The gene order is consistent with other weevil mt genomes in Entiminae, within a typical gene order of "RANSEF". Phylogenetic analysis was performed using 13 protein-coding genes among 18 weevils showed that M. aurolineatus is closely related to another Entiminae species, Sympiezomias velatus.

Are aquatic exercises efficacious in postmenopausal women with knee osteoarthritis? A meta-analysis of randomized controlled trials.

INTRODUCTION: To assess the effects of aquatic exercise in postmenopausal women with knee osteoarthritis using an up-to-date meta-analysis. EVIDENCE ACQUISITION: PubMed, Cochrane Library, Embase, Scopus, Web of Science, Google Scholar, the China National Knowledge Infrastructure (CNKI), the Chinese Biomedical Database (CBM), VIP and Wanfang database were searched systematically for randomized controlled trials (RCTs) published until July 2018. The RCTs included comparing the efficacy of aquatic exercise vs. control in postmenopausal women with knee osteoarthritis, the primary outcomes were assessed by the Western Ontario McMaster University Osteoarthritis Index (WOMAC) and the Knee injury and Osteoarthritis Outcome Score (KOOS). EVIDENCE SYNTHESIS: Six RCTs comprising 432 participants. This meta-analysis revealed that aquatic exercise could significantly relieve the symptom of postmenopausal women with knee osteoarthritis. But there was no significant difference between aquatic exercise program and control group for the improvement of pain, stiffness, function outcomes, sport, activities of daily living and quality of life. CONCLUSIONS: Contrary to prior reviews, our analysis demonstrated that aquatic exercise has no positive impact on pain physical function, stiffness, activities of daily living, sport and quality of life in elderly women with knee osteoarthritis. However, aquatic exercise could improve the symptoms of knee osteoarthritis. Further investigation is needed because of limited available data.

Characterization of the complete mitochondrial genome of the tea leaf roller Caloptilia theivora (Insecta: Lepidoptera: Gracillariidae).

The tea leaf roller, Caloptilia theivora (Walsingham), is a serious pest of tea plants. We have obtained and annotated the complete mitochondrial genome of C. theivora (GenBank accession No. MK541932). The entire mt genome is 15,297 bp long with an A + T content of 80.66%. The mt genome of C. theivora encodes all 37 genes that are typically found in animal mt genomes, consists of 13 protein-coding genes, 2 ribosomal RNA, and 22 transfer RNA genes. The gene order is consistent with other moths mt genome in Ditrysia. The control region of this genome is 192 bp long with a high A + T content of 96.35%, and located between the rrnS and trnI genes. Phylogenetic analysis was performed using 13 protein-coding genes among 19 moths showed that C. theivora is closely related to species of Gracillariidae.

The complete mitochondrial genome of the tea lace bug, Stephanitis chinensis (Hemiptera: Tingidae).

The tea lace bug, Stephanitis chinensis Drake (Hemiptera: Tingidae), is a pest which feeds on the undersides of tea leaves by piercing the epidermis and sucking the sap, and causes great harm to plant growth and tea production. We have obtained the whole mitochondrial genome of S. chinensis (GenBank accession No. MF498769). The entire mt genome is 16,667 bp in size with an A + T content of 78.41%. The tea lace bug mt genome encodes all 37 genes that are typically found in animal mt genomes, consists of 13 protein-coding genes (PCGs), 2 ribosomal RNA, and 22 transfer RNA genes. The gene order is consistent with other sequenced mt genome of lace bugs. The A + T-rich region of this genome is 2215 bp long with the A + T content of 82.58%, and located between the rrnS and trnI genes. Phylogenetic analysis performed using 13 PCGs with 14 heteropteran insects showed that S. chinensis clusters with other Tingidae species.

The complete mitochondrial genome of a tea bagworm, Mahasena colona (Lepidoptera: Psychidae).

The mitochondrial genome of Mahasena colona Sonan has been sequenced and annotated completely. The entire genome is 16,119 bp in length with an A + T content of 82.85% (GenBank accession No. KY856825). The tea bagworm mt genome encodes all 37 genes that are typically found in animal mt genomes, consists of 13 protein-coding genes, 2 ribosomal RNA genes, and 22 transfer RNA genes. Within the mt genome of M. colona, there are six gene reading frame overlaps. The gene order is consistent with other sequenced mt genome of moths and butterflies in Ditrysia. The mt genome of M. colona contains a 728 bp A + T-rich region with a high A + T content of 97.66%.

The complete mitochondrial genome of a tea geometrid, Ectropis obliqua (Lepidoptera: Geometridae).

The tea geometrid, Ectropis obliqua Prout (Lepidoptera: Geometridae), is a major pest of tea plantation and poses a considerable economic threat to tea industry. We have sequenced the complete mitochondrial genome of E. obliqua. The entire genome is 16,535 bp in length with an A + T content of 81.32% (GenBank accession No. KX827002). The tea geometrid mt genome encodes all 37 genes that are typically found in animal mt genomes, consists of 13 protein-coding genes (PCG), two ribosomal RNA genes, and 22 transfer RNA genes. The gene order is consistent with other sequenced mt genome of moths and butterflies in Ditrysia. The A + T-rich region is 1523 bp long and consisting of the motif 'ATAGA', a 19 bp poly-T stretch, and a tandem repeat sequence with seven 194 bp repeat units. Phylogenetic analysis was performed using 13 PCG with 16 moths showed that E. obliqua clusters with other Geometridae species.

The complete mitochondrial genome of the stick tea thrips Dendrothrips minowai (Thysanoptera: Thripidae).

The stick tea thrips, Dendrothrips minowai Priesner (Thysanoptera: Thripidae), is a major pest of tea plantation and poses a considerable economic threat to tea industry. The mitochondrial genome of D. minowai have been sequenced and annotated completely. The entire genome is 14,631 bp in length with an A + T content of 78.53% (GenBank accession No. MF582634). The stick tea thrips mt genome encodes all 37 genes that are typically found in animal mt genomes, consists of 13 protein-coding genes, two ribosomal RNA genes, and 22 transfer RNA genes. The gene order is unique and different from that of the other thrips. The A + T-rich region is 149 bp long and contains two poly-T stretchs. Phylogenetic analysis was performed using 13 protein-coding genes with six thrips showed that D. minowai and other five Thripidae species were clustered into a branch, which is formed a sister clade to H. aculeatus (family Phlaeothripidae).

Effects of Ethacrynic Acid Addition to Diet on Fitness and Development in the Psocid Liposcelis bostrychophila Badonnel.

Fertility life table provides a comprehensive description of arthropod population dynamics by the estimation of parameters about arthropod population growth potential. It can also clarify the sublethal effects of chemicals on insects. Ethacrynic acid (EA), an inhibitor of glutathione S-transferases, is a diuretic compound that has been confirmed to modulate drug resistance in organisms. In this study, the effects of EA on growth and development of Liposcelis bostrychophila Badonnel were investigated in the laboratory to explore the potential possibilities of EA as an active agent to manage insecticide-resistant psocids. The treatment of psocids was obtained by feeding on the routine diet containing 3% EA for three successive generations, and psocids on routine diet served as control. The results indicated that EA possessed some negative effects on the life-table parameters of the psocid in F1 and F2 generations. The addition of EA to diet stunted psocids growth by lengthening development time and increasing mortality with a greater effect in the F2 generation. In the third generation of psocids on EA diet, it seemed there was a return to normal. Psocid fitness was influenced by addition of EA to standard diet. Using rm values, the fitness for EA diet in F1, F2, and F3 compared with the counterpart of routine diet was calculated as 0.80, 0.74, and 0.87, respectively.

The complete mitogenome of Jankowskia athleta (Lepidoptera: Geometridae).

We have sequenced the entire mitochondrial genome of Jankowskia athleta, that is 15 534 bp in length with a 79.53% A + T content (GenBank accession no. KR822683). The mt genome of J. athleta encodes 37 genes that are typically found in metazoan mt genomes, consists of 13 protein-coding genes, 2 ribosomal RNA genes, and 22 transfer RNA genes. Within the mt genome of J. athleta, there are six gene reading frame overlaps. The gene order is consistent with other yet sequenced mt genome in Geometridae. The mt genome of J. athleta has a 475 bp A + T-rich region with an A + T content of 93.47%. The result of phylogenetic analysis infers that J. athleta is more closely related to species of Geometridae than other moths.

The complete mitochondrial genome of a tea pest looper, Buzura suppressaria (Lepidoptera: Geometridae).

We obtained the complete mitochondrial genome of Buzura suppressaria. The mt genome of B. suppressaria is 15,628 bp in length with a 79.43% A + T content (GenBank accession No. KP278206). It encodes 37 mt genes that are typically found in metazoan mt genomes, consisting of protein-coding genes, 2 ribosomal RNA genes and 22 transfer RNA genes. The gene order of the mt genome of B. suppressaria is consistent with other insects in Geometridae. The mt genome of B. suppressaria has a shortest A + T-rich region in Geometridae, that is 348 bp long with an A + T content of 92.82%.

The complete mitochondrial genome of the booklouse, Liposcelis decolor: insights into gene arrangement and genome organization within the genus Liposcelis.

Booklice in the genus Liposcelis are pests of stored grain products. They pose a considerable economic threat to global food security and safety. To date, the complete mitochondrial genome has only been determined for a single booklouse species Liposcelis bostrychophila. Unlike most bilateral animals, which have their 37 mt genes on one circular chromosome, ≈15 kb in size, the mt genome of L. bostrychophila has two circular chromosomes, 8 and 8.5 kb in size. Here, we report the mt genome of another booklouse, Liposcelis decolor. The mt genome of L. decolor has the typical mt chromosome of bilateral animals, 14,405 bp long with 37 genes (13 PCGs, 22 tRNAs and 2 rRNAs). However, the arrangement of these genes in L. decolor differs substantially from that observed in L. bostrychophila and other insects. With the exception of atp8-atp6, L. decolor differs from L. bostrychophila in the arrangement of all of the other 35 genes. The variation in the mt genome organization and mt gene arrangement between the two Liposcelis species is unprecedented for closely related animals in the same genus. Furthermore, our results indicate that the two-chromosome mt genome organization observed in L. bostrychophila likely evolved recently after L. bostrychophila and L. decolor split from their most recent common ancestor.

[Analysis of microsatellite loci from Bactrocera dorsalis based on transcriptome dataset].

The transcriptome database of the oriental fruit fly, Bactrocera dorsalis (Hendel), was used to identify the functional gene-microsatellite (EST-SSR) markers and to analyze the SSR loci information. In total, 1890 EST-SSR loci were identified, of which, 1296 SSR sequences could be used for primer design. The average distribution frequency of the transcriptomic SSRs was 1/10. 21 kb. However, these distribution frequencies varied considerably among different types of repeat SSRs. The tri-nucleotide repeat SSRs were found to have the highest frequency among the different types of repeat SSRs in the EST-SSR of B. dorsalis. Combining with other literatures, we inferred that the tri-nucleotide repeat SSRs were the most abundant EST-SSR in all of insects. In this study, 42 pairs of EST-SSR primers were designed and 18 pairs produced amplification bands of expected sizes. According to the results of other related literatures, the practices and challenges of strategy for SSR isolation from insect transcriptome databases were discussed, and the problems which should be considered in the screening of insect transcriptomic EST-SSR were put forward.

Molecular characterization and alternative splicing of a sodium channel and DSC1 ortholog genes in Liposcelis bostrychophila Badonnel (Psocoptera: Liposcelididae).

Alternative splicing greatly contributes to the structural and functional diversity of voltage-gated sodium channels (VGSCs) by generating various isoforms with unique functional and pharmacological properties. Here, we identified a new optional exon 23 located in the linker between domains II and III, and four mutually exclusive exons (exons 27A, 27B, 27C, and 27D) in domains IIIS3 and IIIS4 of the sodium channel of Liposcelis bostrychophila (termed as LbVGSC). This suggested that more alternative splicing phenomena remained to be discovered in VGSCs. Inclusion of exon 27C might lead to generation of non-functional isoforms. Meanwhile, identification of three alternative exons (exons 11, 13A, and 13B), which were located in the linker between domains II and III, indicated that abundant splicing events occurred in the DSC1 ortholog channel of L. bostrychophila (termed as LbSC1). Exons 13A and 13B were generated by intron retention, and the presence of exon 13B relied on the inclusion of exon 13A. Exon 13B was specifically expressed in the embryonic stage and contained an in-frame stop codon, inclusion of which led to generation of truncated proteins with only the first two domains. Additionally, several co-occurring RNA editing events were identified in LbSC1. Furthermore, remarkable similarity between the structure and expression patterns of LbVGSC and LbSC1 were discovered, and a closer evolutionary relationship between VGSCs and DSC1 orthologs was verified. Taken together, the data provided abundant molecular information on VGSC and DSC1 orthologs in L. bostrychophila, a representative Psocoptera storage pest, and insights into the alternative splicing of these two channels.