WUSCHEL-related homeobox family genes in rice control lateral root primordium size.

The development of a plastic root system is essential for stable crop production under variable environments. Rice plants have two types of lateral roots (LRs): S-type (short and thin) and L-type (long, thick, and capable of further branching). LR types are determined at the primordium stage, with a larger primordium size in L-types than S-types. Despite the importance of LR types for rice adaptability to variable water conditions, molecular mechanisms underlying the primordium size control of LRs are unknown. Here, we show that two WUSCHEL-related homeobox (WOX) genes have opposing roles in controlling LR primordium (LRP) size in rice. Root tip excision on seminal roots induced L-type LR formation with wider primordia formed from an early developmental stage. QHB/OsWOX5 was isolated as a causative gene of a mutant that is defective in S-type LR formation but produces more L-type LRs than wild-type (WT) plants following root tip excision. A transcriptome analysis revealed that OsWOX10 is highly up-regulated in L-type LRPs. OsWOX10 overexpression in LRPs increased the LR diameter in an expression-dependent manner. Conversely, the mutation in OsWOX10 decreased the L-type LR diameter under mild drought conditions. The qhb mutants had higher OsWOX10 expression than WT after root tip excision. A yeast one-hybrid assay revealed that the transcriptional repressive activity of QHB was lost in qhb mutants. An electrophoresis mobility shift assay revealed that OsWOX10 is a potential target of QHB. These data suggest that QHB represses LR diameter increase, repressing OsWOX10 Our findings could help improve root system plasticity under variable environments.

PIN2/3/4 auxin carriers mediate root growth inhibition under conditions of boron deprivation in Arabidopsis.

The mechanistic basis by which boron (B) deprivation inhibits root growth via the mediation of root apical auxin transport and distribution remains elusive. This study showed that B deprivation repressed root growth of wild-type Arabidopsis seedlings, which was related to higher auxin accumulation (observed with DII-VENUS and DR5-GFP lines) in B-deprived roots. Boron deprivation elevated the auxin content in the root apex, coinciding with upregulation of the expression levels of auxin biosynthesis-related genes (TAA1, YUC3, YUC9, and NIT1) in shoots, but not in root apices. Phenotyping experiments using auxin transport-related mutants revealed that the PIN2/3/4 carriers are involved in root growth inhibition caused by B deprivation. B deprivation not only upregulated the transcriptional levels of PIN2/3/4, but also restrained the endocytosis of PIN2/3/4 carriers (observed with PIN-Dendra2 lines), resulting in elevated protein levels of PIN2/3/4 in the plasma membrane. Overall, these results suggest that B deprivation not only enhances auxin biosynthesis in shoots by elevating the expression levels of auxin biosynthesis-related genes but also promotes the polar auxin transport from shoots to roots by upregulating the gene expression levels of PIN2/3/4, as well as restraining the endocytosis of PIN2/3/4 carriers, ultimately resulting in auxin accumulation in root apices and root growth inhibition.

Identification and Characterization of LBD Gene Family in Pseudoroegneria libanotica Reveals Functions of PseLBD1 and PseLBD12 in Response to Abiotic Stress.

The lateral organ boundaries domain (LBD) plays a vital role as a transcriptional coactivator within plants, serving as an indispensable function in growth, development, and stress response. In a previous study, we found that the LBD genes of Pseudoroegneria libanotica (a maternal donor for three-quarter of perennial Triticeae species with good stress resistance, holds great significance in exploring its response mechanisms to abiotic stress for the Triticeae tribe) might be involved in responding to drought stress. Therefore, we further identified the LBD gene family in this study. A total of 29 PseLBDs were identified. Among them, 24 were categorized into subclass I, while 5 fell into subclass II. The identification of cis-acting elements reveals the extensive involvement of PseLBDs in various biological processes in P. libanotica. Collinearity analysis indicates that 86% of PseLBDs were single-copy genes and have undergone a single whole-genome duplication event. Transcriptomic differential expression analysis of PseLBDs under drought stress reveals that the most likely candidates for responding to abiotic stress were PseLBD1 and PseLBD12. They have been demonstrated to respond to drought, salt, heavy metal, and heat stress in yeast. Furthermore, it is plausible that functional divergence might have occurred among their orthologous genes in wheat. This study not only establishes a foundation for a deeper understanding of the biological roles of PseLBDs in P. libanotica but also unveils novel potential genes for enhancing the genetic background of crops within Triticeae crops, such as wheat.

Complete mitochondrial genome of Agrostis stolonifera: insights into structure, Codon usage, repeats, and RNA editing.

BACKGROUND: Plants possess mitochondrial genomes that are large and complex compared to animals. Despite their size, plant mitochondrial genomes do not contain significantly more genes than their animal counterparts. Studies into the sequence and structure of plant mitochondrial genomes heavily imply that the main mechanism driving replication of plant mtDNA, and offer valuable insights into plant evolution, energy production, and environmental adaptation. RESULTS: This study presents the first comprehensive analysis of Agrostis stolonifera's mitochondrial genome, characterized by a branched structure comprising three contiguous chromosomes, totaling 560,800 bp with a GC content of 44.07%. Annotations reveal 33 unique protein-coding genes (PCGs), 19 tRNA genes, and 3 rRNA genes. The predominant codons for alanine and glutamine are GCU and CAA, respectively, while cysteine and phenylalanine exhibit weaker codon usage biases. The mitogenome contains 73, 34, and 23 simple sequence repeats (SSRs) on chromosomes 1, 2, and 3, respectively. Chromosome 1 exhibits the most frequent A-repeat monomeric SSR, whereas chromosome 2 displays the most common U-repeat monomeric SSR. DNA transformation analysis identifies 48 homologous fragments between the mitogenome and chloroplast genome, representing 3.41% of the mitogenome's total length. The PREP suite detects 460 C-U RNA editing events across 33 mitochondrial PCGs, with the highest count in the ccmFn gene and the lowest in the rps7 gene. Phylogenetic analysis confirms A. stolonifera's placement within the Pooideae subfamily, showing a close relationship to Lolium perenne, consistent with the APG IV classification system. Numerous homologous co-linear blocks are observed in A. stolonifera's mitogenomes and those of related species, while certain regions lack homology. CONCLUSIONS: The unique features and complexities of the A. stolonifera mitochondrial genome, along with its similarities and differences to related species, provide valuable insights into plant evolution, energy production, and environmental adaptation. The findings from this study significantly contribute to the growing body of knowledge on plant mitochondrial genomes and their role in plant biology.

Uncovering early transcriptional regulation during adventitious root formation in Medicago sativa.

BACKGROUND: Alfalfa (Medicago sativa L.) as an important legume plant can quickly produce adventitious roots (ARs) to form new plants by cutting. But the regulatory mechanism of AR formation in alfalfa remains unclear. RESULTS: To better understand the rooting process of alfalfa cuttings, plant materials from four stages, including initial separation stage (C stage), induction stage (Y stage), AR primordium formation stage (P stage) and AR maturation stage (S stage) were collected and used for RNA-Seq. Meanwhile, three candidate genes (SAUR, VAN3 and EGLC) were selected to explore their roles in AR formation. The numbers of differentially expressed genes (DEGs) of Y-vs-C (9,724) and P-vs-Y groups (6,836) were larger than that of S-vs-P group (150), indicating highly active in the early AR formation during the complicated development process. Pathways related to cell wall and sugar metabolism, root development, cell cycle, stem cell, and protease were identified, indicating that these genes were involved in AR production. A large number of hormone-related genes associated with the formation of alfalfa ARs have also been identified, in which auxin, ABA and brassinosteroids are thought to play key regulatory roles. Comparing with TF database, it was found that AP2/ERF-ERF, bHLH, WRKY, NAC, MYB, C2H2, bZIP, GRAS played a major regulatory role in the production of ARs of alfalfa. Furthermore, three identified genes showed significant promotion effect on AR formation. CONCLUSIONS: Stimulation of stem basal cells in alfalfa by cutting induced AR production through the regulation of various hormones, transcription factors and kinases. This study provides new insights of AR formation in alfalfa and enriches gene resources in crop planting and cultivation.

Exploring root system architecture and anatomical variability in alfalfa (Medicago sativa L.) seedlings.

BACKGROUND: The growth of alfalfa (Medicago sativa L.) is significantly hampered by drought and nutrient deficiencies. The identification of root architectural and anatomical characteristics holds paramount importance for the development of alfalfa genotypes with enhanced adaptation to adverse environmental conditions. In this study, we employed a visual rhizobox system to investigate the variability in root system architecture (including root depth, root length, root tips number, etc.), anatomical features (such as cortical traits, total stele area, number and area of vessel, etc.), as well as nitrogen and phosphorus uptake across 53 alfalfa genotypes during the seedling stage. RESULTS: Out of the 42 traits measured, 21 root traits, along with nitrogen (N) and phosphorus (P) uptake, displayed higher coefficients of variation (CVs ≥ 0.25) among the tested genotypes. Local root morphological and anatomical traits exhibited more significant variation than global root traits. Twenty-three traits with CVs ≥ 0.25 constituted to six principal components (eigenvalues > 1), collectively accounting for 88.0% of the overall genotypic variation. Traits such as total root length, number of root tips, maximal root depth, and others exhibited positive correlations with shoot dry mass and root dry mass. Additionally, total stele area and xylem vessel area showed positive correlations with N and P uptake. CONCLUSIONS: These root traits, which have demonstrated associations with biomass and nutrient uptake, may be considered for the breeding of alfalfa genotypes that possess efficient resource absorption and increased adaptability to abiotic stress, following validation during the entire growth period in the field.

A chromosome-level genome assembly of radish (Raphanus sativus L.) reveals insights into genome adaptation and differential bolting regulation.

High-quality radish (Raphanus sativus) genome represents a valuable resource for agronomical trait improvements and understanding genome evolution among Brassicaceae species. However, existing radish genome assembly remains fragmentary, which greatly hampered functional genomics research and genome-assisted breeding. Here, using a NAU-LB radish inbred line, we generated a reference genome of 476.32 Mb with a scaffold N50 of 56.88 Mb by incorporating Illumina, PacBio and BioNano optical mapping techniques. Utilizing Hi-C data, 448.12 Mb (94.08%) of the assembled sequences were anchored to nine radish chromosomes with 40 306 protein-coding genes annotated. In total, 249.14 Mb (52.31%) comprised the repetitive sequences, among which long terminal repeats (LTRs, 30.31%) were the most abundant class. Beyond confirming the whole-genome triplication (WGT) event in R. sativus lineage, we found several tandem arrayed genes were involved in stress response process, which may account for the distinctive phenotype of high disease resistance in R. sativus. By comparing against the existing Xin-li-mei radish genome, a total of 2 108 573 SNPs, 7740 large insertions, 7757 deletions and 84 inversions were identified. Interestingly, a 647-bp insertion in the promoter of RsVRN1 gene can be directly bound by the DOF transcription repressor RsCDF3, resulting into its low promoter activity and late-bolting phenotype of NAU-LB cultivar. Importantly, introgression of this 647-bp insertion allele, RsVRN1In-536 , into early-bolting genotype could contribute to delayed bolting time, indicating that it is a potential genetic resource for radish late-bolting breeding. Together, this genome resource provides valuable information to facilitate comparative genomic analysis and accelerate genome-guided breeding and improvement in radish.

Agronomic and ionomics indicators of high-yield, mineral-dense, and low-Cd grains of wheat (Triticum aestivum L.) cultivars.

The accumulation of toxic and essential nutrient elements in wheat grain influences wheat yield, grain nutritional quality, and human health. Here, we assessed the potential for breeding wheat cultivars to combine high yield with low cadmium and high iron and/or zinc concentrations in grains, and we screened appropriate cultivars. A pot experiment was conducted to explore differences in grain cadmium, iron, and zinc concentrations among 68 wheat cultivars, as well as their relationships with other nutrient elements and agronomic characters. The results showed 2.04-, 1.71-, and 1.64-fold differences in grain cadmium, iron, and zinc concentrations, respectively, among the 68 cultivars. Grain cadmium concentration was positively correlated with grain zinc, iron, magnesium, phosphorus, and manganese concentrations. Grain copper concentration was positively correlated with grain zinc and iron concentrations, but not with grain cadmium concentration. Therefore, copper has a potential role in regulating grain iron and zinc accumulation without influencing cadmium concentration in wheat grain. There were no significant relationships between grain cadmium concentration and four important wheat agronomic characters (i.e., grain yield, straw yield, thousand kernel weight, and plant height), indicating that the breeding of low-cadmium-accumulating cultivars with dwarfism and high yield characteristics is possible. On cluster analysis, four cultivars (Ningmai11, Xumai35, Baomai6, and Aikang58) exhibited low-cadmium and high-yield characteristics. Among them, Aikang58 contained moderate iron and zinc concentrations, while Ningmai11 had relatively high iron but low zinc concentrations in the grain. These results imply that it is feasible to breed high-yield dwarf wheat with low cadmium and moderate iron and zinc concentrations in the grain.

The role of root size and root efficiency in grain production, and water-and nitrogen-use efficiency in wheat.

BACKGROUND: The root system is the major plant organ involved in water and nutrient acquisition, influencing plant growth and productivity. However, the relative importance of root size and uptake efficiency remains undetermined. A pot experiment was conducted using two wheat varieties with different root sizes to evaluate their capacity for water and nitrogen (N) uptake and their effects on grain production, water-use efficiency (WUE), and N-use efficiency (NUE) under two water treatments combined with three N levels. RESULTS: The leaf water potential and root exudates of changhan58 (CH, small root variety) were higher or similar to those of changwu134 (CW, large root variety) under water/N treatment combinations, indicating that small roots can transport enough water to above the ground. The addition of N improved plant growth, photosynthetic traits, and WUE significantly. There were no significant differences in WUE or grain production between the two cultivars under well-watered conditions. However, they were significantly higher in CH than in CW under water deficit stress. Nitrogen uptake per unit root dry weight, glutaminase, and nitrate reductase activities were significantly higher in CH than in CW, regardless of moisture conditions. Root biomass was positively correlated with evapotranspiration, while the root/shoot ratio was negatively correlated with WUE (P < 0.05) but not with NUE. CONCLUSION: In a pot experiment, water and N uptake were more strongly associated with resource uptake availability than root size. This may provide guidance in wheat breeding programs for drought-prone regions. © 2023 Society of Chemical Industry.

Influence of biochar-based urea substituting urea on rice yield, bacterial community and nitrogen cycling in paddy fields.

BACKGROUND: There is an increasing understanding of the importance of biochar-based fertilizers in agroecosystems. However, no research has evaluated the effects of partial substitution of urea with biochar-based urea on rice yields and soil microbial communities. We therefore investigated the rice yields, bacterial communities, and gene abundance involved in nitrogen in silty clay and sandy loam soil paddy fields treated with urea (U), total substitution of urea with biochar-based urea (BSU), partial substitution of urea with biochar-based urea in basal and tillering fertilizers (BSU1), and partial substitution of urea with biochar-based urea in panicle fertilizers (BSU2). RESULTS: Compared with U, applying biochar-based urea increased rice yields, with BSU2 having the most notable effect. Principal coordinate analysis revealed that bacterial communities treated with BSU2 in both soils were significantly different from those treated with U and BSU, most probably due to the decrease in pH caused by the decrease in the concentration of ammonium. The relative abundance of Subdivision3_genera_incertae_sedis, Azotobacter, Geobacter, Buchnera, and Terrimonas in silty clay soils and Saccharibacteria_genera_incertae_sedis and Geobacter in sandy loam soils significantly increased when treated with BSU2 and was positively correlated with rice yields, indicating that the improvements in rice yield were associated with changes in bacterial communities. Based upon amoA/narG related to nitrate accumulation and norB/nosZ related to nitrous oxide emissions, BSU2 enabled a lower risk of nitrate leaching and nitrous oxide emissions in both soils, in comparison with the U and BSU treatments. CONCLUSION: The BSU2 treatment had a stronger yield-increasing effect than biochar-based urea alone and lowered the risk of nitrogen pollution, which is beneficial to the sustainable development of paddy fields. © 2022 Society of Chemical Industry.

Genotypic variation in the tolerance to moderate cadmium toxicity among 20 maize genotypes with contrasting root systems.

BACKGROUND: Cadmium (Cd) contamination in farmland is a serious environmental and safety issue affecting plant growth, crop productivity, and human health. This study aimed to investigate genotypic variation in root morphology and Cd accumulations under moderate Cd stress among diverse maize genotypes. Twenty maize genotypes with contrasting root systems were assessed for Cd tolerance 39 days after transplanting (V6, six-leaf stage) under 20 µmol L-1 CdCl2 using a semi-hydroponic phenotyping platform in a glasshouse. RESULTS: Cadmium stress significantly inhibited plant growth across all genotypes. Genotypic variation in response to Cd toxicity was apparent: shoot dry weight varied from 0.13 (genotype NS2020) to 0.35 g plant-1 (Dongke301) with deductions up to 63% compared with non-Cd treatment (CK). Root dry weight of 20 genotypes ranged from 0.06 (NS2020) to 0.18 g plant-1 (Dongke301) with a deduction up to 56%. Root length ranged from 2.21 (NS590b) to 9.22 m (Dongke301) with a maximal decline of 76%. Cadmium-treated genotypes generally had thicker roots and average diameter increased by 34% compared with CK. Genotypes had up to 3.25 and 3.50 times differences in shoot and root Cd concentrations, respectively. Principal component and cluster analyses assigned the 20 genotypes into Cd-tolerant (five genotypes) and Cd-sensitive (15 genotypes) groups. CONCLUSIONS: Maize genotypes varied significantly in response to moderate Cd stress. Cadmium-tolerant genotypes optimized root morphology and Cd accumulation and distribution. This study could assist in the selection and breeding of new cultivars with improved adaptation to Cd-contaminated soil for food and feed or land remediation purposes. © 2022 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Combined analysis of mRNA and miRNA reveals the banana potassium absorption regulatory network and validation of miRNA160a.

Potassium (K) has an important effect on the growth and development of plants. Banana contains higher K content than many other fruits, and its plant requires more K nutrient in soil. However, the soil in the banana-producing areas in China is generally deficient in K. Therefore, understanding the mechanism of banana K absorption may assist in providing effective strategy to solve this problem. This study used two banana varieties with contrasting K tolerance, 'Guijiao No. 1' (low-K tolerant), and 'Brazilian banana' (low-K sensitive)to investigate K absorption mechanisms in response to low-K stress through miRNA and mRNA sequencing analysis. Under low-K condition, 'Guijiao No.1' showed higher plant height, dry weight, tissue K content and ATPase activity. Analysis of transcription factors showed that they were mainly in the types or classes of MYB, AP-EREBP, bHLH, etc. The sequencing results showed that 'Guijiao No. 1' had 776 differentially expressed genes (DEGs) and 27 differentially expressed miRNAs (DEMs), and 'Brazilian banana' had 71 DEGs and 14 DEMs between normal and low K treatments. RT-qPCR results showed that all miRNAs and mRNAs showed similar expression patterns with RNA-Seq and transcriptome. miRNA regulatory network was constructed by integrated analysis of miRNA-mRNA data. miR160a was screened out as a key miRNA, and preliminary functional validation was performed. Arabidopsis overexpressing miR160a showed reduced tolerance to low K, and inhibited phenotypic traits such as shorter root length, and reduced K accumulation. The overexpressed miR160a had a targeting relationship with ARF10 and ARF16 in Arabidopsis. These results indicate that miR160a may regulate K absorption in bananas through the auxin pathway. This study provides a theoretical basis for further study on the molecular mechanism of banana response to low potassium stress.

QTL analyses of soybean root system architecture revealed genetic relationships with shoot-related traits.

KEY MESSAGE: The genetic basis of soybean root system architecture (RSA) and the genetic relationship between shoot and RSA were revealed by integrating data from recombinant inbred population grafting and QTL mapping. Variations in root system architecture (RSA) affect the functions of roots and thus play vital roles in plant adaptations and agricultural productivity. The aim of this study was to unravel the genetic relationship between RSA traits and shoot-related traits in soybean. This study characterized RSA variability at seedling stage in a recombinant inbred population, derived from a cross between cultivated soybean C08 and wild soybean W05, and performed high-resolution quantitative trait locus (QTL) mapping. In total, 34 and 41 QTLs were detected for RSA-related and shoot-related traits, respectively, constituting eight QTL clusters. Significant QTL correspondence was found between shoot biomass and RSA-related traits, consistent with significant correlations between these phenotypes. RSA-related QTLs also overlapped with selection regions in the genome, suggesting the cultivar RSA could be a partial consequence of domestication. Using reciprocal grafting, we confirmed that shoot-derived signals affected root development and the effects were controlled by multiple loci. Meanwhile, RSA-related QTLs were found to co-localize with four soybean flowering-time loci. Consistent with the phenotypes of the parental lines of our RI population, diminishing the function of flowering controlling E1 family through RNA interference (RNAi) led to reduced root growth. This implies that the flowering time-related genes within the RSA-related QTLs are actually contributing to RSA. To conclude, this study identified the QTLs that determine RSA through controlling root growth indirectly via regulating shoot functions, and discovered superior alleles from wild soybean that could be used to improve the root structure in existing soybean cultivars.

Differentially reset transcriptomes and genome bias response orchestrate wheat response to phosphate deficiency.

Phosphorus (P) is an essential macronutrient for all organisms. Phosphate (Pi) deficiency reduces grain yield and quality in wheat. Understanding how wheat responds to Pi deficiency at the global transcriptional level remains limited. We revisited the available RNA-seq transcriptome from Pi-starved wheat roots and shoots subjected to Pi starvation. Genome-wide transcriptome resetting was observed under Pi starvation, with a total of 917 and 2338 genes being differentially expressed in roots and shoots, respectively. Chromosomal distribution analysis of the gene triplets and differentially expressed genes (DEGs) revealed that the D genome displayed genome induction bias and, specifically, the chromosome 2D might be a key contributor to Pi-limiting triggered gene expression response. Alterations in multiple metabolic pathways pertaining to secondary metabolites, transcription factors and Pi uptake-related genes were evidenced. This study provides genomic insight and the dynamic landscape of the transcriptional changes contributing to the hexaploid wheat during Pi starvation. The outcomes of this study and the follow-up experiments have the potential to assist the development of Pi-efficient wheat cultivars.

Nitrogen supply improved plant growth and Cd translocation in maize at the silking and physiological maturity under moderate Cd stress.

Soil cadmium (Cd) contamination is a serious problem on agricultural land. Adequate nitrogen (N) may help ameliorate plant fitness under Cd stress. This study examined the role of N application in improving maize tolerance to Cd stress. Two maize genotypes, Zhongke11 (larger root system) and Shengrui999 (smaller root system), were grown in a loessal soil amended with Cd (Cd0, no added Cd; Cd1, 20 mg kg-1 soil as CdCl2·2.5 H2O) and N (N0, no added N; N1, 100 mg kg-1 soil as urea) under greenhouse, and plants were assessed at silking and maturity stages. Maize plants exhibited moderate Cd stress with significantly reduced grain yield, especially under low N (N1). Roots accumulated more Cd than above-ground parts. Grain Cd concentration was the least (0.05-0.06 µg g-1) among all organs which is below the safety threshold. Leaf Cd concentrations (0.24-1.18 mg kg-1) were also under the toxicity threshold. Nitrogen addition significantly improved plant growth, chlorophyll content, photosynthesis traits, and tissue Cd contents, and reduced Cd concentration in soil compared to N0 treatment. Nitrogen promoted Cd bioconcentration and translocation factors in stem and leaves. Cadmium stress reduced N fertilizer agronomic efficiency at maturity. At maturity, root Cd content was positively correlated with root N and calcium accumulation, and stem Cd content was positively correlated with stem N content (both P ≤ 0.05). Genotypes with different root system size differed in response to Cd toxicity and / or N deficit. The small-rooted genotype Shengrui999 was more tolerant to moderate Cd stress than the large-rooted Zhongke11. Addition of N ameliorated Cd stress in both maize genotypes by improving plant growth performance, and regulating Cd translocations among plant organs.

Phytochemical Profiling, Antioxidant, Anti-Inflammatory, Thrombolytic, Hemolytic Activity In Vitro and In Silico Potential of Portulacaria afra.

The use of complementary herbal medicines has recently increased in an attempt to find effective alternative therapies that reduce the adverse effects of chemical drugs. Portulacaria afra is a rich source of phytochemicals with high antioxidant activity, and thus may possess health benefits. This study used the latest developments in GC-MS coupling with molecular docking techniques to identify and quantify the phytoconstituents in P. afra tissue extracts. The results revealed that n-butanol P. afra (BUT-PA) dry extracts contained total phenolic and flavonoids contents of 21.69 ± 0.28 mgGAE/g and 196.58 ± 6.29 mgGAE/g, respectively. The significant potential of antioxidants was observed through CUPRIC, FRAP, and ABTS methods while the DPPH method showed a moderate antioxidants potential for P. afra. Enzymatic antioxidants, superoxide dismutase, peroxidase and catalase also showed a better response in the BUT-PA dry extracts. The thrombolytic activity of the BUT-PA extracts ranged from 0.4 ± 0.32 to 11.2 ± 0.05%. Similarly, hemolytic activity ranged from 5.76 ± 0.15 to 9.26 ± 0.15% using the standard (triton x) method. The BUTPA and CHPA showed moderate acetylcholinesterase and butrylcholinesterase inhibition, ranging from 40.78 ± 0.52 to 58.97 ± 0.33, compared to galantamine. The carrageenan induced hind-paw edema assay, while BUT-PA extracts showed anti-inflammatory properties in a dose-dependent manner. Furthermore, 20 compounds were identified in the BUTPA extracts by GC-MS. Molecular docking was performed to explore the synergistic effect of the GC-MS-identified compounds on COX-1 and COX-2 inhibition. A high binding affinity was observed for Stigmastan-3, 5-diene, Phthalic acid, 3. Alpha-Hydroxy-5, 16-androstenol. The computed binding energies of the compounds revealed that all the compounds have a synergistic effect, preventing inflammation. It was concluded that active phytochemicals were present in P. afra, with the potential for multiple pharmacological applications as a latent source of pharmaceutically important compounds. This should be further explored to isolate secondary metabolites that can be employed in the treatment of different diseases.

Lateral root elongation enhances nitrogen-use efficiency in maize genotypes at the seedling stage.

BACKGROUND: Maize plants show great variation in root morphological response to nitrogen (N) deficit, and such alterations often determine N-use efficiency (NUE) plants. This study assessed genotypic variation in root morphology and NUE in selected 20 maize genotypes with contrasting root system size grown in a semi-hydroponic phenotyping system for 38 days under control (4 mmol L-1 NO3 - ) and low N (LN) (40 µmol L-1 ) for 38 days after transplanting. RESULTS: Maize genotypes exhibited different responses to LN stress in each of the 28 measured shoot and root traits. The 20 genotypes were assigned into one of the three groups: N-efficient (eight genotypes), medium (four genotypes), and N-inefficient (eight genotypes), based on shoot dry weight ratio (the ratio of shoot dry weight at LN and control) ± one standard error. In response to LN stress, the N-inefficient genotypes had significant reduction in biomass production by ~58% in shoots and ~64% in roots, while the N-efficient genotypes maintained their biomass. Under LN supply N-efficient genotypes showed a plasticity response that would result in both sparse lateral branching and increased root elongation as a whole or at each growth strata, and N efficiency positively correlated with lateral or axial root elongation and root elongation at different depths. CONCLUTSION: The total lateral root length was the main contributor to the improved N foraging and utilization in maize under LN conditions, followed by axial root length. Total lateral root length can be considered in breeding programs for producing maize cultivars with high NUE at the early seedling stage. © 2022 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Arbuscular mycorrhizal symbioses alleviating salt stress in maize is associated with a decline in root-to-leaf gradient of Na+/K+ ratio.

BACKGROUND: Inoculation of arbuscular mycorrhizal (AM) fungi has the potential to alleviate salt stress in host plants through the mitigation of ionic imbalance. However, inoculation effects vary, and the underlying mechanisms remain unclear. Two maize genotypes (JD52, salt-tolerant with large root system, and FSY1, salt-sensitive with small root system) inoculated with or without AM fungus Funneliformis mosseae were grown in pots containing soil amended with 0 or 100 mM NaCl (incrementally added 32 days after sowing, DAS) in a greenhouse. Plants were assessed 59 DAS for plant growth, tissue Na+ and K+ contents, the expression of plant transporter genes responsible for Na+ and/or K+ uptake, translocation or compartmentation, and chloroplast ultrastructure alterations. RESULTS: Under 100 mM NaCl, AM plants of both genotypes grew better with denser root systems than non-AM plants. Relative to non-AM plants, the accumulation of Na+ and K+ was decreased in AM plant shoots but increased in AM roots with a decrease in the shoot: root Na+ ratio particularly in FSY1, accompanied by differential regulation of ion transporter genes (i.e., ZmSOS1, ZmHKT1, and ZmNHX). This induced a relatively higher Na+ efflux (recirculating) rate than K+ in AM shoots while the converse outcoming (higher Na+ influx rate than K+) in AM roots. The higher K+: Na+ ratio in AM shoots contributed to the maintenance of structural and functional integrity of chloroplasts in mesophyll cells. CONCLUSION: AM symbiosis improved maize salt tolerance by accelerating Na+ shoot-to-root translocation rate and mediating Na+/K+ distribution between shoots and roots.

Elevated temperature and waterlogging decrease cottonseed quality by altering the accumulation and distribution of carbohydrates, oil and protein.

Soil waterlogging and high-temperature events have occurred simultaneously in recent years in the Yangtze River basin cotton belt region of China, negatively affecting the development and quality of cottonseed. This study investigated the effects of the combination of elevated temperature (ET) (34.1/29.0°C) and waterlogging (3 or 6 days) on the accumulation and distribution of oil, protein and carbohydrates in cottonseed during flowering and boll development. The results showed that ET resulted in greater decreases in cottonseed biomass under waterlogging than under control conditions. The combination of waterlogging and ET significantly limited the accumulation of carbohydrates and oil contents. However, ET promoted protein accumulation and compensated for the negative effects of 3-day waterlogging on the final protein content. The combined ET and 6-day waterlogging significantly decreased the final contents of oil and protein by limiting carbon flux and NADPH supply because of the decreased activities of phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31) and glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49). The PEPC activity was correlated more with protein content than oil content. In addition, simultaneous exposure to waterlogging and ET resulted in lower unsaturated fatty acid/saturated fatty acid ratios and essential amino acid/non-essential amino acid ratios than did exposure to the individual factors alone. These findings could provide the theoretical support for the prospective assessment of effects of high temperature and waterlogging stresses on cotton production under climate change, and they can help to develop effective techniques in cotton cultivation.

Root system architecture, physiological and transcriptional traits of soybean (Glycine max L.) in response to water deficit: A review.

Drought stress is the main limiting factor for global soybean growth and production. Genetic improvement for water and nutrient uptake efficiency is critical to advance tolerance and enable more sustainable and resilient production, underpinning yield growth. The identification of quantitative traits and genes related to water and nutrient uptake will enhance our understanding of the mechanisms of drought tolerance in soybean. This review summarizes drought stress in the context of the physiological traits that enable effective acclimation, with a particular focus on roots. Genes controlling root system architecture play an important role in water and nutrient availability, and therefore important targets for breeding strategies to improve drought tolerance. This review highlights the candidate genes that have been identified as regulators of important root traits and responses to water stress. Progress in our understanding of the function of particular genes, including GmACX1, GmMS and GmPEPCK are discussed in the context of developing a system-based platform for genetic improvement of drought tolerance in soybean.