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1.
Mol Cell ; 75(3): 605-619.e6, 2019 08 08.
Artigo em Inglês | MEDLINE | ID: mdl-31255466

RESUMO

Accurate DNA replication is essential to preserve genomic integrity and prevent chromosomal instability-associated diseases including cancer. Key to this process is the cells' ability to stabilize and restart stalled replication forks. Here, we show that the EXD2 nuclease is essential to this process. EXD2 recruitment to stressed forks suppresses their degradation by restraining excessive fork regression. Accordingly, EXD2 deficiency leads to fork collapse, hypersensitivity to replication inhibitors, and genomic instability. Impeding fork regression by inactivation of SMARCAL1 or removal of RECQ1's inhibition in EXD2-/- cells restores efficient fork restart and genome stability. Moreover, purified EXD2 efficiently processes substrates mimicking regressed forks. Thus, this work identifies a mechanism underpinned by EXD2's nuclease activity, by which cells balance fork regression with fork restoration to maintain genome stability. Interestingly, from a clinical perspective, we discover that EXD2's depletion is synthetic lethal with mutations in BRCA1/2, implying a non-redundant role in replication fork protection.


Assuntos
DNA Helicases/genética , Replicação do DNA/genética , Exodesoxirribonucleases/genética , RecQ Helicases/genética , Proteína BRCA1/genética , Proteína BRCA2/genética , Instabilidade Genômica/genética , Células HeLa , Humanos , Neoplasias/genética , Mutações Sintéticas Letais/genética
2.
Nucleic Acids Res ; 50(13): 7493-7510, 2022 07 22.
Artigo em Inglês | MEDLINE | ID: mdl-35819196

RESUMO

Cellular senescence triggers various types of heterochromatin remodeling that contribute to aging. However, the age-related mechanisms that lead to these epigenetic alterations remain elusive. Here, we asked how two key aging hallmarks, telomere shortening and constitutive heterochromatin loss, are mechanistically connected during senescence. We show that, at the onset of senescence, pericentromeric heterochromatin is specifically dismantled consisting of chromatin decondensation, accumulation of DNA breakages, illegitimate recombination and loss of DNA. This process is caused by telomere shortening or genotoxic stress by a sequence of events starting from TP53-dependent downregulation of the telomere protective protein TRF2. The resulting loss of TRF2 at pericentromeres triggers DNA breaks activating ATM, which in turn leads to heterochromatin decondensation by releasing KAP1 and Lamin B1, recombination and satellite DNA excision found in the cytosol associated with cGAS. This TP53-TRF2 axis activates the interferon response and the formation of chromosome rearrangements when the cells escape the senescent growth arrest. Overall, these results reveal the role of TP53 as pericentromeric disassembler and define the basic principles of how a TP53-dependent senescence inducer hierarchically leads to selective pericentromeric dismantling through the downregulation of TRF2.


Assuntos
Senescência Celular , Centrômero , Heterocromatina , Encurtamento do Telômero , Proteína Supressora de Tumor p53/metabolismo , Linhagem Celular , Cromatina , Dano ao DNA , Regulação para Baixo , Células HeLa , Humanos , Telômero/genética , Proteína 2 de Ligação a Repetições Teloméricas/metabolismo
3.
Nat Commun ; 14(1): 2428, 2023 04 27.
Artigo em Inglês | MEDLINE | ID: mdl-37105990

RESUMO

Telomerase-independent cancer proliferation via the alternative lengthening of telomeres (ALT) relies upon two distinct, largely uncharacterized, break-induced-replication (BIR) processes. How cancer cells initiate and regulate these terminal repair mechanisms is unknown. Here, we establish that the EXD2 nuclease is recruited to ALT telomeres to direct their maintenance. We demonstrate that EXD2 loss leads to telomere shortening, elevated telomeric sister chromatid exchanges, C-circle formation as well as BIR-mediated telomeric replication. We discover that EXD2 fork-processing activity triggers a switch between RAD52-dependent and -independent ALT-associated BIR. The latter is suppressed by EXD2 but depends specifically on the fork remodeler SMARCAL1 and the MUS81 nuclease. Thus, our findings suggest that processing of stalled replication forks orchestrates elongation pathway choice at ALT telomeres. Finally, we show that co-depletion of EXD2 with BLM, DNA2 or POLD3 confers synthetic lethality in ALT cells, identifying EXD2 as a potential druggable target for ALT-reliant cancers.


Assuntos
Neoplasias , Telomerase , Humanos , Homeostase do Telômero , Replicação do DNA , Encurtamento do Telômero , Reparo do DNA , Telomerase/genética , Telômero/genética , Telômero/metabolismo , Neoplasias/genética , DNA Helicases/genética , DNA Helicases/metabolismo
4.
Curr Opin Genet Dev ; 60: 69-76, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-32193147

RESUMO

At the crossroads of DNA damage repair and genomic instability, telomere research significantly expands our knowledge on fundamental mechanisms involved in cancer initiation and progression, pledging novel tools for targeted and universal onco-therapies. Molecular cytogenetics through the application of a battery of fluorescent hybridization technologies plays an important role toward understanding telomere homeostasis. Herein, we review distinct molecular cytogenetic phenotypes associated with telomere repair, functionality, and elongation. We discuss the underlying mechanisms responsible for their formation or repair, focusing on Break-induced-Replication (BIR)-mediated conservative telomeric neo-synthesis, recently shown to drive the enigmatic Alternative Lengthening of Telomeres in neoplasia.


Assuntos
Dano ao DNA , Reparo do DNA , Instabilidade Genômica , Microscopia/métodos , Neoplasias/genética , Neoplasias/patologia , Telômero , Análise Citogenética , Replicação do DNA , Humanos
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