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1.
Nature ; 543(7646): 519-524, 2017 03 23.
Artigo em Inglês | MEDLINE | ID: mdl-28273065

RESUMO

The organization of the genome in the nucleus and the interactions of genes with their regulatory elements are key features of transcriptional control and their disruption can cause disease. Here we report a genome-wide method, genome architecture mapping (GAM), for measuring chromatin contacts and other features of three-dimensional chromatin topology on the basis of sequencing DNA from a large collection of thin nuclear sections. We apply GAM to mouse embryonic stem cells and identify enrichment for specific interactions between active genes and enhancers across very large genomic distances using a mathematical model termed SLICE (statistical inference of co-segregation). GAM also reveals an abundance of three-way contacts across the genome, especially between regions that are highly transcribed or contain super-enhancers, providing a level of insight into genome architecture that, owing to the technical limitations of current technologies, has previously remained unattainable. Furthermore, GAM highlights a role for gene-expression-specific contacts in organizing the genome in mammalian nuclei.


Assuntos
Cromatina/genética , Cromatina/metabolismo , Mapeamento Cromossômico , Elementos Facilitadores Genéticos/genética , Genoma/genética , Animais , Cromatina/química , Epigênese Genética , Masculino , Camundongos , Modelos Genéticos , Células-Tronco Embrionárias Murinas/citologia , Células-Tronco Embrionárias Murinas/metabolismo , Análise de Sequência de DNA , Transcrição Gênica/genética
2.
Genes Dev ; 23(1): 105-17, 2009 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-19136628

RESUMO

Genomic imprinting requires the differential marking by DNA methylation of genes in male and female gametes. In the female germline, acquisition of methylation imprint marks depends upon the de novo methyltransferase Dnmt3a and its cofactor Dnmt3L, but the reasons why specific sequences are targets for Dnmt3a and Dnmt3L are still poorly understood. Here, we investigate the role of transcription in establishing maternal germline methylation marks. We show that at the Gnas locus, truncating transcripts from the furthest upstream Nesp promoter disrupts oocyte-derived methylation of the differentially methylated regions (DMRs). Transcription through DMRs in oocytes is not restricted to this locus but occurs across the prospective DMRs at many other maternally marked imprinted domains, suggesting a common requirement for transcription events. The transcripts implicated here in gametic methylation are protein-coding, in contrast to the noncoding antisense transcripts involved in the monoallelic silencing of imprinted genes in somatic tissues, although they often initiate from alternative promoters in oocytes. We propose that transcription is a third essential component of the de novo methylation system, which includes optimal CpG spacing and histone modifications, and may be required to create or maintain open chromatin domains to allow the methylation complex access to its preferred targets.


Assuntos
Metilação de DNA/fisiologia , Impressão Genômica/genética , Oócitos/metabolismo , Transcrição Gênica/genética , Alelos , Animais , Cromograninas , Feminino , Subunidades alfa Gs de Proteínas de Ligação ao GTP/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Dados de Sequência Molecular
3.
Proc Natl Acad Sci U S A ; 109(40): 16173-8, 2012 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-22988072

RESUMO

Chromatin has a complex spatial organization in the cell nucleus that serves vital functional purposes. A variety of chromatin folding conformations has been detected by single-cell imaging and chromosome conformation capture-based approaches. However, a unified quantitative framework describing spatial chromatin organization is still lacking. Here, we explore the "strings and binders switch" model to explain the origin and variety of chromatin behaviors that coexist and dynamically change within living cells. This simple polymer model recapitulates the scaling properties of chromatin folding reported experimentally in different cellular systems, the fractal state of chromatin, the processes of domain formation, and looping out. Additionally, the strings and binders switch model reproduces the recently proposed "fractal-globule" model, but only as one of many possible transient conformations.


Assuntos
Montagem e Desmontagem da Cromatina/fisiologia , Cromatina/química , Regulação da Expressão Gênica/genética , Genoma/genética , Modelos Químicos , Simulação por Computador , Hibridização in Situ Fluorescente , Método de Monte Carlo
4.
Biochem Soc Trans ; 41(2): 508-12, 2013 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-23514144

RESUMO

In the cell nucleus, chromosomes have a complex spatial organization, spanning several length scales, which serves vital functional purposes. It is unknown, however, how their three-dimensional architecture is orchestrated. In the present article, we review the application of a model based on classical polymer physics, the strings and binders switch model, to explain the molecular mechanisms of chromatin self-organization. We explore the scenario where chromatin architecture is shaped and regulated by the interactions of chromosomes with diffusing DNA-binding factors via thermodynamics mechanisms and compare it with available experimental data.


Assuntos
Cromatina/metabolismo , Modelos Biológicos , Animais , Humanos
5.
Adv Exp Med Biol ; 695: 14-25, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-21222196

RESUMO

Fundamental features of genome regulation depend on the linear DNA sequence, cell type specific modification of DNA and chromatin-associated proteins, which locally control the expression of single genes. Architectural features of genome organization within the three-dimensional (3D) nuclear space establish preferential positioning of genes relative to nuclear subcompartments associated with specific biochemical activities, thereby influencing states of expression. The structural and temporal organization of the genome within the nucleus of stem cells, together with specific features of epigenetic and transcriptional regulation are emerging as key players that influence pluripotency and differentiation.1,2.


Assuntos
Núcleo Celular , Genoma , Diferenciação Celular/genética , Núcleo Celular/metabolismo , Cromatina/metabolismo , Regulação da Expressão Gênica , Células-Tronco
7.
Elife ; 42015 Dec 19.
Artigo em Inglês | MEDLINE | ID: mdl-26687004

RESUMO

Dynamic post-translational modification of RNA polymerase II (RNAPII) coordinates the co-transcriptional recruitment of enzymatic complexes that regulate chromatin states and processing of nascent RNA. Extensive phosphorylation of serine residues at the largest RNAPII subunit occurs at its structurally-disordered C-terminal domain (CTD), which is composed of multiple heptapeptide repeats with consensus sequence Y1-S2-P3-T4-S5-P6-S7. Serine-5 and Serine-7 phosphorylation mark transcription initiation, whereas Serine-2 phosphorylation coincides with productive elongation. In vertebrates, the CTD has eight non-canonical substitutions of Serine-7 into Lysine-7, which can be acetylated (K7ac). Here, we describe mono- and di-methylation of CTD Lysine-7 residues (K7me1 and K7me2). K7me1 and K7me2 are observed during the earliest transcription stages and precede or accompany Serine-5 and Serine-7 phosphorylation. In contrast, K7ac is associated with RNAPII elongation, Serine-2 phosphorylation and mRNA expression. We identify an unexpected balance between RNAPII K7 methylation and acetylation at gene promoters, which fine-tunes gene expression levels.


Assuntos
Regulação da Expressão Gênica , Lisina/metabolismo , Processamento de Proteína Pós-Traducional , RNA Polimerase II/metabolismo , Transcrição Gênica , Animais , Consenso , Metilação , Camundongos , Células NIH 3T3 , Fosforilação , Serina/metabolismo
8.
Noncoding RNA ; 1(3): 246-265, 2015 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-29861426

RESUMO

Macro long non-coding RNAs (lncRNAs) play major roles in gene silencing in inprinted gene clusters. Within the imprinted Gnas cluster, the paternally expressed Nespas lncRNA downregulates its sense counterpart Nesp. To explore the mechanism of action of Nespas, we generated two new knock-in alleles to truncate Nespas upstream and downstream of the Nesp promoter. We show that Nespas is essential for methylation of the Nesp differentially methylated region (DMR), but higher levels of Nespas are required for methylation than are needed for downregulation of Nesp. Although Nespas is transcribed for over 27 kb, only Nespas transcript/transcription across a 2.6 kb region that includes the Nesp promoter is necessary for methylation of the Nesp DMR. In both mutants, the levels of Nespas were extraordinarily high, due at least in part to increased stability, an effect not seen with other imprinted lncRNAs. However, even when levels were greatly raised, Nespas remained exclusively cis-acting. We propose Nespas regulates Nesp methylation and expression to ensure appropriate levels of expression of the protein coding transcripts Gnasxl and Gnas on the paternal chromosome. Thus, Nespas mediates paternal gene expression over the entire Gnas cluster via a single gene, Nesp.

9.
Nucleus ; 4(4): 267-73, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23823730

RESUMO

The underlying global organization of chromatin within the cell nucleus has been the focus of intense recent research. Hi-C methods have allowed for the detection of genome-wide chromatin interactions, revealing a complex large-scale organization where chromosomes tend to partition into megabase-sized "topological domains" of local chromatin interactions and intra-chromosomal contacts extends over much longer scales, in a cell-type and chromosome specific manner. Until recently, the distinct chromatin folding properties observed experimentally have been difficult to explain in a single conceptual framework. We reported that a simple polymer-physics model of chromatin, the strings and binders switch (SBS) model, succeeds in describing the full range of chromatin configurations observed in vivo. The SBS model simulates the interactions between randomly diffusing binding molecules and binding sites on a polymer chain. It explains how polymer architectural patterns can be established, how different stable conformations can be produced and how conformational changes can be reliably regulated by simple strategies, such as protein upregulation or epigenetic modifications, via fundamental thermodynamics mechanisms.


Assuntos
Cromatina/química , Cromatina/metabolismo , Polímeros/química , Núcleo Celular , Humanos , Modelos Moleculares , Polímeros/metabolismo
10.
Cold Spring Harb Perspect Biol ; 2(6): a000588, 2010 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-20484389

RESUMO

Eukaryotic gene expression is an intricate multistep process, regulated within the cell nucleus through the activation or repression of RNA synthesis, processing, cytoplasmic export, and translation into protein. The major regulators of gene expression are chromatin remodeling and transcription machineries that are locally recruited to genes. However, enzymatic activities that act on genes are not ubiquitously distributed throughout the nucleoplasm, but limited to specific and spatially defined foci that promote preferred higher-order chromatin arrangements. The positioning of genes within the nuclear landscape relative to specific functional landmarks plays an important role in gene regulation and disease.


Assuntos
Regulação da Expressão Gênica/fisiologia , Ordem dos Genes/genética , Ordem dos Genes/fisiologia , Cromatina/genética , Doenças Genéticas Inatas/genética , Predisposição Genética para Doença
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