Amdoparvovirus-associated disease in striped skunks (Mephitis mephitis).

Disease caused by the archetypical amdoparvovirus (APV), Aleutian mink disease virus (AMDV), has been well studied, but APV infections in other carnivores are poorly understood. Skunk amdoparvovirus (SKAV), one of a handful of newly discovered APVs, is apparently species-specific in striped skunks (Mephitis mephitis) and has a high prevalence across North America. We have evaluated the infection status and viral tissue distribution in a cohort of 26 free-ranging California skunks from a single rehabilitation facility who were euthanized due to poor prognosis for recovery from neurologic disease. SKAV was detected in the majority of this cohort, and virus was associated with a spectrum of lesions including tubulointerstitial nephritis, meningoencephalitis, myocarditis, and arteritis. Affected tissue and patterns of inflammation were partially overlapping with those of AMDV infection but were notably distinct in the kidney.

Prevalence of risky driving behaviours on popular television series

Summary: Motor vehicle crashes are a leading cause of death among young adults. Social media and television have been shown to affect the likelihood that young adults will engage in risk-taking behaviour. We watched 216 episodes of five popular television series on Netflix and identified 333 separate driving scenes, of which 271 (81.4%) portrayed at least one risky driving behaviour. Unsafe driving (not wearing a seat belt) was the most common risky driving behaviour noted, occurring in 245 (73.6%) of driving scenes. Distracted driving (36 [18.8%]) and driving while using a cellphone (28 [8.4%]) were also noted. Popular television series model unsafe driving behaviours. Seat belts are infrequently used. As well, drivers are often distracted, looking away from the road to talk or talking on their cellphones. Television producers should be sensitive to modelling unsafe driving behaviours, particularly if the audience consists largely of young people.

Congenital glycogen storage disease in a South American coati (Nasua nasua).

A 14-mo-old South American coati (Nasua nasua) was submitted for necropsy to the University of Kentucky Veterinary Diagnostic Laboratory. The coati had a history of progressive neurologic signs beginning 3 mo prior to euthanasia. At necropsy, the coati was in thin body condition, but no other significant findings were evident. Histopathologic findings included moderate distension of neuronal cell bodies by finely vesiculated cytoplasm within the cerebrum, cerebellum, spinal cord, and intestinal ganglia. Hepatocytes and macrophages in the lung, spleen, and liver were similarly affected. Transmission electron microscopy showed numerous electrondense membranous cytoplasmic bodies, swirls, and vesicular profiles within neuronal lysosomes in the brain. To the authors' knowledge, this is the first report of a naturally occurring congenital glycogen storage disease in a South American coati and the family Procyonidae.

Global climatological data of ocean thermohaline parameters derived from WOA18.

This is a global ocean climatological dataset of 17 thermohaline parameters such as isothermal layer (ITL) depth (hT), mixed layer (ML) depth (hD), thermocline gradient (GT), pycnocline gradient (GD) determined from temperature (T) and salinity (S) profiles of the National Centers for Environmental Information (NCEI) world ocean atlas 2018 (WOA18) using the double gradient method along with the identity-index (i-index) showing the quality of the determination. With the identified (hT, hD) and (GT, GD), other parameters such as ITL heat content (HITL), mixed layer fresh-water content (FML), maximum thermocline gradient (GTmax), thermocline depth (hth), temperature at thermocline depth (Tth), maximum density gradient GDmax), pycnocline depth (hpyc), density at pycnocline depth (ρpyc), and (hT - hD) (barrier layer if positive or compensated layer if negative). The dataset is located at the NOAA/NCEI website ( https://doi.org/10.25921/j3v2-jy50 ). It provides useful background information for ocean mixed layer dynamics, air-sea interaction, climatological studies. This paper is the only document for the dataset.

Equine abortion and stillbirth in California: a review of 1,774 cases received at a diagnostic laboratory, 1990-2022.

Reproductive failure represents an important cause of economic loss for the equine industry. We reviewed the cases of equine abortion and stillbirth submitted to the California Animal Health and Food Safety Laboratory System, University of California-Davis from 1990 to 2022. A total of 1,774 cases were reviewed. A confirmed cause of abortion was determined in 29.2% of the cases. Abortion or stillbirth was attributed to infectious agents in 18.7% of the cases, with Streptococcus spp., equine herpesvirus 1, and Leptospira spp. being the most prevalent. Noninfectious causes of abortion were established in 10.5% of the cases, with umbilical cord torsion being the most common. In 70.8% of the cases, a definitive cause of abortion could not be established. Our study demonstrated the difficulties in establishing an etiologic diagnosis, even when following a standard diagnostic work-up. New diagnostic approaches are needed to improve the likelihood of reaching a final diagnosis in cases of equine abortion and stillbirth.

A review of epidemiology and public health control measures of COVID-19 variants in Hong Kong, December 2020 to June 2021.

Background: The emergence of coronavirus disease 2019 (COVID-19) variants posed considerable threats to the global public health. We reviewed the epidemiology of variant cases and control measures implemented in Hong Kong. Methods: The epidemiological characteristics and the temporal trend of the COVID-19 variant cases and local clusters in Hong Kong, and the corresponding public health control measures were reviewed. Results: Between December 2020 and June 2021, 393 variant cases were reported, including 153, 59 and 79 cases of Alpha, Beta and Delta variants with no Gamma variant. The vast majority (378, 96.2%) were imported cases. Since early June 2021, Delta variant had taken over Alpha as the dominant strain. Public health control measures, including risk-stratified quarantine and testing requirements for inbound travellers, banning of flights from extremely high-risk areas, enhanced contact tracing and quarantine, were implemented. Among the 3 clusters involving local transmissions, 2 were linked to imported cases while the source of the remaining one was unknown. Discussion: Amid the global surges of COVID-19 variants, Hong Kong had continued to limit and prevent the occurrence of community-wide outbreak. Ongoing control strategies should be constantly reviewed and adjusted in response to the global and local COVID-19 situation.

Nanostructure and surface composition of Pt and Ru binary catalysts on polyaniline-functionalized carbon nanotubes.

Pt-Ru binary catalysts were prepared on a polyaniline-functionalized multiwalled carbon nanotube (PANi/MWCNT). PANi/MWCNT composites were synthesized by the polymerization of aniline in the presence of a carbon nanotube suspension using FeSO(4) and (NH(4))(2)S(2)O(8) as the oxidants. The Pt-Ru/PANi/MWCNT catalysts were formed by the chemical reduction of H(2)PtCl(6) and RuCl(3) using NaBH(4) as the reducing agent. The binary component catalyst is sharply distributed, with particle sizes ranging from 2.0 to 4.0 nm, and the Pt and Ru distributions are homogeneous when supported on PANi/MWCNT. In comparison, the binary catalyst supported on bare MWCNT displayed a Pt-rich core and a Ru-rich shell nanostructure. The surface composition deduced from CO stripping potentials confirms that the Ru surface content (χ(Ru)) is approximately 50% for the Pt-Ru alloy on PANi/MWCNT, and the catalyst on bare MWCNT shows nearly 70% Ru on the surface. Pt-Ru binary catalysts supported on PANi/MWCNT have higher activity, a higher Pt utilization efficiency, and much better durability when compared to other catalyst supports on bare MWCNT or on Vulcan XC-72.

Ocean dynamic equations with the real gravity.

Two different treatments in ocean dynamics are found between the gravity and pressure gradient force. Vertical component is 5-6 orders of magnitude larger than horizontal components for the pressure gradient force in large-scale motion, and for the gravity in any scale motion. The horizontal pressure gradient force is considered as a dominant force in oceanic motion from planetary to small scales. However, the horizontal gravity is omitted in oceanography completely. A non-dimensional C number (ratio between the horizontal gravity and the Coriolis force) is used to identify the importance of horizontal gravity in the ocean dynamics. Unexpectedly large C number with the global mean around 24 is obtained using the community datasets of the marine geoid height and ocean surface currents. New large-scale ocean dynamic equations with the real gravity are presented such as hydrostatic balance, geostrophic equilibrium, thermal wind, equipotential coordinate system, and vorticity equation.

A Complete Formula of Ocean Surface Absolute Geostrophic Current.

Absolute geostrophic current at the ocean surface (S) contains three components: (1) absolute geostrophic current at the geoid undulation (N), (2) geostrophic shear (between S and N) due to horizontal gradient of dynamic ocean topography, and (3) geostrophic shear (between S and N) due to horizontal gradient of density. However, only the second component is used to represent the surface absolute geostrophic current in physical oceanography and satellite geodesy. Four public datasets are used to calculate each component. Evident difference between the surface absolute geostrophic current and the second component (i.e., geostrophic shear due to horizontal gradient of dynamic ocean topography) was found by the statistical characteristics with the relative root mean square difference of 0.605. It shows that the common practice in physical oceanography and satellite geodesy is not correct. The horizontal gradient of dynamic ocean topography alone cannot be claimed as the surface absolute geostrophic current.

Characterization of a subpopulation of colon cancer cells with stem cell-like properties.

The biology of the normal colonic mucosa suggests that colon cancer originates from normal colon stem cells. CD44 cancer stem cells have been identified in breast and prostate cancer, and we therefore examined whether CD44 similarly identified colon cancer stem cells. Initial assays found CD44(hi) colon tumor cells to have enhanced soft agar colony-forming ability. Subsequently, CD44(hi) cells isolated from 4 primary colon adenocarcinoma xenografts were found to be highly tumorigenic in immune deficient mice. CD44(hi) cells consistently formed tumors with 1,000 cells, and in multiple experiments, as few as 10 and 100 CD44(hi) cells formed tumors in 7/10 and 21/28 mice, respectively. In contrast, CD44(-) colon tumor cells were either nontumorigenic or 10-50-fold less tumorigenic. CD44(hi) cells could be serially passaged up to 4 times in vivo, suggesting self-renewal capacity, and formed tumors that recapitulated the heterogeneity of the original patient tumor. CD44(hi) cells were significantly enriched for nuclear activated beta-catenin, a key element in normal stem/progenitor cells and in early colon tumor progression. Bromodeoxyuridine (BrdU) labeling studies indicated that CD44(hi) cells divide slowly relative to the CD44(-) cells, suggesting their tumorigenicity is not simply due to faster proliferation. Aldehyde dehydrogenase (ALDH) sort further increased the tumorigenicity of CD44(hi) cells from 2/2 patient tumors, but CD133 tumor cells in our hands did not have increased tumorigenicity. Our observations indicate that CD44 is a marker of stem-like cells in colon cancer, and support the use of additional markers to further purify colon cancer stem cells.

Global ocean synoptic thermocline gradient, isothermal-layer depth, and other upper ocean parameters.

Different from the existing global ocean climatological datasets of isothermal layer (ITL) depth (h), a global ocean synoptic dataset of h along with other parameters has been established from temperature profiles of the National Centers for Environmental Information (NCEI) world ocean database 1961-2017. The exponential leap-forward gradient method was used to identify h and thermocline gradient (G) from each temperature profile measured by expendable bathythermograph (XBT) and conductivity, temperature, depth (CTD) instruments. Due to quality and vertical resolution of the profiling data, the numbers of (G, h) pairs are 446,811 out of 964,942 CTD profiles and 755,086 out of 2,303,433 XBT profiles. With the given h, other parameters such as ITL heat content (HITL), sea surface temperature (SST), temperature below ITL (Tm), and quality measures (Q, I) indices are provided. Altogether, the dataset contains 1,201,897 temporally and horizontally varying sets of (G, h, HITL, SST, Tm, Q-index, I-index). Note that we added 200° to the longitude. This synoptic dataset is located on the NCEI website for public use.

Large-scale mapping of human protein-protein interactions by mass spectrometry.

Mapping protein-protein interactions is an invaluable tool for understanding protein function. Here, we report the first large-scale study of protein-protein interactions in human cells using a mass spectrometry-based approach. The study maps protein interactions for 338 bait proteins that were selected based on known or suspected disease and functional associations. Large-scale immunoprecipitation of Flag-tagged versions of these proteins followed by LC-ESI-MS/MS analysis resulted in the identification of 24,540 potential protein interactions. False positives and redundant hits were filtered out using empirical criteria and a calculated interaction confidence score, producing a data set of 6463 interactions between 2235 distinct proteins. This data set was further cross-validated using previously published and predicted human protein interactions. In-depth mining of the data set shows that it represents a valuable source of novel protein-protein interactions with relevance to human diseases. In addition, via our preliminary analysis, we report many novel protein interactions and pathway associations.

Design and analysis of quantitative differential proteomics investigations using LC-MS technology.

Liquid chromatography-mass spectrometry (LC-MS)-based proteomics is becoming an increasingly important tool in characterizing the abundance of proteins in biological samples of various types and across conditions. Effects of disease or drug treatments on protein abundance are of particular interest for the characterization of biological processes and the identification of biomarkers. Although state-of-the-art instrumentation is available to make high-quality measurements and commercially available software is available to process the data, the complexity of the technology and data presents challenges for bioinformaticians and statisticians. Here, we describe a pipeline for the analysis of quantitative LC-MS data. Key components of this pipeline include experimental design (sample pooling, blocking, and randomization) as well as deconvolution and alignment of mass chromatograms to generate a matrix of molecular abundance profiles. An important challenge in LC-MS-based quantitation is to be able to accurately identify and assign abundance measurements to members of protein families. To address this issue, we implement a novel statistical method for inferring the relative abundance of related members of protein families from tryptic peptide intensities. This pipeline has been used to analyze quantitative LC-MS data from multiple biomarker discovery projects. We illustrate our pipeline here with examples from two of these studies, and show that the pipeline constitutes a complete workable framework for LC-MS-based differential quantitation. Supplementary material is available at http://iec01.mie.utoronto.ca/~thodoros/Bukhman/.

Afforestation for reduction of NOX concentration in Lanzhou China.

Lanzhou is one of the major industrial cities in northwest China, the capital of Gansu Province, and located at a northwest-to-southeast oriented valley basin with elevation about 1500-1600-m. Due to topographic and meteorological characteristics, Lanzhou is one of the most polluted cities in China. Meteorological conditions (low winds, stable stratification especially inversion), pollutant sources and sinks affect the air quality. Lanzhou government carried out afforestation and pollutant-source reduction (closing several heavy industrial factories) to improve the air quality for the past two decades. In this study, effect of afforestation on reducing the NO(X) concentration is investigated numerically using RAMS-HYPACT model.

Particulate air pollution in Lanzhou China.

Concentrations of total suspended particles (TSP) and PM(10) in Lanzhou China have been kept high for the past two decades. Data collected during the intensive observational period from October 1999 to April 2001 show high TSP and PM(10) concentrations. Starting from November, the PM(10) pollution intensifies, and reaches mid to high alert level of air pollution, continues until April next year, and is at low alert level in the summer. In the winter and spring, the TSP concentration is 2-10 times higher than the third-level criterion of air quality (severe pollution). Effects of intrinsic factors (sources of pollution) and remote preconditions (propagation of dust storms) for severe PM(10) and TSP pollution in Lanzhou are analyzed.

CT scanning for diagnosing blunt ureteral and ureteropelvic junction injuries.

BACKGROUND: Blunt ureteral and ureteropelvic (UPJ) injuries are extremely rare and very difficult to diagnose. Many of these injuries are missed by the initial trauma evaluation. METHODS: Trauma registry data was used to identify all blunt trauma patients with ureteral or UPJ injuries, from 1 April 2001 to 30 November 2006. Demographics, injury information and outcomes were determined. Chart review was then performed to record initial clinical and all CT findings. RESULTS: Eight patients had ureteral or UPJ injuries. Subtle findings such as perinephric stranding and hematomas, and low density retroperitoneal fluid were evident on all initial scans, and prompted delayed excretory scans in 7/8 cases. As a result, ureteral and UPJ injuries were diagnosed immediately for these seven patients. These findings were initially missed in the eighth patient because significant associated visceral findings mandated emergency laparotomy. All ureteral and UPJ injuries have completely healed except for the case with the delay in diagnosis. CONCLUSION: Most blunt ureteral and UPJ injuries can be identified if delayed excretory CT scans are performed based on initial CT findings of perinephric stranding and hematomas, or the finding of low density retroperitoneal fluid.

Metalloprotease inhibitors block release of soluble CD27 and enhance the immune stimulatory activity of chronic lymphocytic leukemia cells.

OBJECTIVE: Chronic lymphocytic leukemia (CLL) B cells from most patients express both membrane-bound CD27 (mCD27) and soluble CD27 (sCD27). Expression of sCD27 inhibits CD27-dependent T-cell or CLL-cell activation mediated by its ligand, CD70. In this study, we evaluated whether protease inhibitors could inhibit the release of sCD27 from CLL cells and enhance T-cell activation mediated by CD27-CD70 interaction. METHODS: CLL cells exposed to hydroxamic acid-based matrix metalloprotease (MMP) inhibitors were evaluated for the release of sCD27 by sandwich enzyme-linked immunosorbent assay and immunoprecipitation. We examined for phenotypic changes in CLL cells treated with MMP inhibitors by flow cytometry and T-cell activation by CLL cells was assessed by [(3)H] thymidine incorporation assay and the production of interferon-gamma. RESULTS: Treatment of CLL cells with MMP inhibitors blocked the release of sCD27 to the culture supernatant. In contrast, a non-hydroxamic acid control compound or inhibitors of other proteases, including serine, cysteine, and aspartyl proteases, were ineffective. Furthermore, CLL cells treated with MMP inhibitors expressed significantly higher levels of accessory molecules, such as CD54, CD80, and CD95. Consistent with such changes, we found that CLL cells treated with MMP inhibitors, but not control treated cells, could stimulate allogeneic and autologous T cells in mixed lymphocyte reactions. CONCLUSION: These data reveal that metalloprotease inhibitors can block production of sCD27, which can interfere with mCD27-CD70 interactions that induce expression of immune costimulatory molecules on CLL B cells. Conceivably, treatment of CLL cells with metalloprotease inhibitors may enhance their potential for stimulating cellular immune recognition of leukemia-associated antigens.