RESUMO
Collagen gels are widely used in experiments on cell mechanics because they mimic the extracellular matrix in physiological conditions. Collagen gels are often characterized by their bulk rheology; however, variations in the collagen fiber microstructure and cell adhesion forces cause the mechanical properties to be inhomogeneous at the cellular scale. We study the mechanics of type I collagen on the scale of tens to hundreds of microns by using holographic optical tweezers to apply pN forces to microparticles embedded in the collagen fiber network. We find that in response to optical forces, particle displacements are inhomogeneous, anisotropic, and asymmetric. Gels prepared at 21 °C and 37 °C show qualitative difference in their micromechanical characteristics. We also demonstrate that contracting cells remodel the micromechanics of their surrounding extracellular matrix in a strain- and distance-dependent manner. To further understand the micromechanics of cellularized extracellular matrix, we have constructed a computational model which reproduces the main experiment findings.
Assuntos
Biopolímeros/química , Colágeno Tipo I/química , Animais , Anisotropia , Linhagem Celular Tumoral , Progressão da Doença , Elasticidade , Matriz Extracelular/metabolismo , Fibroblastos/metabolismo , Géis/química , Humanos , Camundongos , Microscopia Confocal , Células NIH 3T3 , Neoplasias/patologia , Distribuição Normal , Pinças Ópticas , Óptica e Fotônica , Estresse Mecânico , Temperatura , CicatrizaçãoRESUMO
We present a general approach for optimizing the diffraction efficiency of a phase-type spatial light modulator (SLM). While the SLM displays a one-dimensional phase grating, the phase shift of one pixel in the grating is varied and the first-order diffraction efficiency is measured. This is repeated pixel-by-pixel to find the optimum phase encoding for the device that maximizes the diffraction efficiency. This method compensates for nonlinearity of the modulator phase response and is especially useful for optimizing modulators with less than 2π phase shift.