RESUMO
To provide new insight into the dark fermentation process, a multi-lateral study was performed to study the microbiology of 20 different lab-scale bioreactors operated in four different countries (Brazil, Chile, Mexico, and Uruguay). Samples (29) were collected from bioreactors with different configurations, operation conditions, and performances. The microbial communities were analyzed using 16S rRNA genes 454 pyrosequencing. The results showed notably uneven communities with a high predominance of a particular genus. The phylum Firmicutes predominated in most of the samples, but the phyla Thermotogae or Proteobacteria dominated in a few samples. Genera from three physiological groups were detected: high-yield hydrogen producers (Clostridium, Kosmotoga, Enterobacter), fermenters with low-hydrogen yield (mostly from Veillonelaceae), and competitors (Lactobacillus). Inocula, reactor configurations, and substrates influence the microbial communities. This is the first joint effort that evaluates hydrogen-producing reactors and operational conditions from different countries and contributes to understand the dark fermentation process.
Assuntos
Reatores Biológicos/normas , Fermentação , Hidrogênio/metabolismo , Consórcios Microbianos/genética , RNA Ribossômico 16S/genética , Anaerobiose , Clostridium/classificação , Clostridium/genética , Clostridium/metabolismo , Enterobacter/classificação , Enterobacter/genética , Enterobacter/metabolismo , Firmicutes/classificação , Firmicutes/genética , Firmicutes/metabolismo , Sequenciamento de Nucleotídeos em Larga Escala , Lactobacillus/classificação , Lactobacillus/genética , Lactobacillus/metabolismo , América Latina , Proteobactérias/classificação , Proteobactérias/genética , Proteobactérias/metabolismo , Thermotoga maritima/classificação , Thermotoga maritima/genética , Thermotoga maritima/metabolismo , Veillonellaceae/classificação , Veillonellaceae/genética , Veillonellaceae/metabolismoRESUMO
Acinetobacter lactucae OTEC-02 was isolated from hydrocarbon-contaminated soils. Whole-genome sequence analysis was performed to learn more about the strain's ability to degrade different types of recalcitrant toxic monoaromatic hydrocarbons. The genome of this bacterium revealed its genomic properties and versatile metabolic features, as well as a complete prophage.