The Sphagnome Project: enabling ecological and evolutionary insights through a genus-level sequencing project.

Considerable progress has been made in ecological and evolutionary genetics with studies demonstrating how genes underlying plant and microbial traits can influence adaptation and even 'extend' to influence community structure and ecosystem level processes. Progress in this area is limited to model systems with deep genetic and genomic resources that often have negligible ecological impact or interest. Thus, important linkages between genetic adaptations and their consequences at organismal and ecological scales are often lacking. Here we introduce the Sphagnome Project, which incorporates genomics into a long-running history of Sphagnum research that has documented unparalleled contributions to peatland ecology, carbon sequestration, biogeochemistry, microbiome research, niche construction, and ecosystem engineering. The Sphagnome Project encompasses a genus-level sequencing effort that represents a new type of model system driven not only by genetic tractability, but by ecologically relevant questions and hypotheses.

How many of the digits in a mean of 12.3456789012 are worth reporting?

OBJECTIVE: A computer program tells me that a mean value is 12.3456789012, but how many of these digits are significant (the rest being random junk)? Should I report: 12.3?, 12.3456?, or even 10 (if only the first digit is significant)? There are several rules-of-thumb but, surprisingly (given that the problem is so common in science), none seem to be evidence-based. RESULTS: Here I show how the significance of a digit in a particular decade of a mean depends on the standard error of the mean (SEM). I define an index, DM that can be plotted in graphs. From these a simple evidence-based rule for the number of significant digits ('sigdigs') is distilled: the last sigdig in the mean is in the same decade as the first or second non-zero digit in the SEM. As example, for mean 34.63 ± SEM 25.62, with n = 17, the reported value should be 35 ± 26. Digits beyond these contain little or no useful information, and should not be reported lest they damage your credibility.

Feedback control of the rate of peat formation.

The role of peatlands in the global carbon cycle is confounded by two inconsistencies. First, peatlands have been a large reservoir for carbon sequestered in the past, but may be either net sources or net sinks at present. Second, long-term rates of peat accumulation (and hence carbon sequestration) are surprisingly steady, despite great variability in the short-term rates of peat formation. Here, we present a feedback mechanism that can explain how fine-scale and short-term variability in peat-forming processes is constrained to give steady rates of peat accumulation over longer time-scales. The feedback mechanism depends on a humpbacked relationship between the rate of peat formation and the thickness of the aerobic surface layer (the acrotelm), such that individual microforms (hummocks, lawns, hollows and pools) expand or contract vertically in response to fluctuations in the position of the water table. Hummocks (but not hollows) 'evolve' to a steady state where changes in acrotelm thickness compensate for climate-mediated variations in surface wetness. With long-term growth of a topographically confined peat deposit, the steady state gradually shifts to a thicker acrotelm (i.e. taller hummocks) and lower rates of peat formation and carbon sequestration.

UPWASH AND DOWNWASH OF POLLEN AND SPORES IN THE UNSATURATED SURFACE LAYER OF SPHANGNUM-DOMINATED PEAT.

The vertical movement of Corylus and Cedrus pollen and of Lycopodium spores in Sphagnum and peat was studied in a factorial experiment with four flow regimes (no flow, downward, upward, alternate down and up), three injection depths (1, 5 and 9 cm below the surface) and three forms of packing (natural, hand-packed at natural density, haphazard). The experiment lasted for 36 d. The results were corrected for compaction and differential recovery of pollen and spores, but the main results were unaffected by these corrections. Downward, upward and alternating flows moved the median pollen position by about 1.5 cm during the experiment. The flows also increased the interquartile span by 2 cm: about 25 % of grains moved at least 3 cm. The velocity of upward flow is probably more than sufficient to overcome the rate of sinking of pollen and spores in the unsaturated zone. Reversals of flow may be of importance in dislodging grains into the main channels again.