RESUMO
N-myristoyltransferase (NMT) catalyzes the myristoylation of proteins involved in signal transduction, cellular transformation, differentiation, proliferation and oncogenesis. In this study, we report for the first time on the elevated NMT activity in oral squamous cell carcinoma (OSCC). Increased activity is marked with increased staining for NMT in the OSCC samples compared to the normal adjacent tissues. In addition, we observed increased staining for the N-myristoyltransferase inhibitor protein 71 (NIP71) in the OSCC samples compared to the control tissues. These findings suggest the regulatory relationship between NMT and NIP71 during tumorigenesis. It is possible that the increased activity results in the overexpression of NIP71 in an effort to control NMT activity.
Assuntos
Aciltransferases/metabolismo , Carcinoma de Células Escamosas/enzimologia , Neoplasias Bucais/enzimologia , Proteínas de Neoplasias/metabolismo , Carcinoma de Células Escamosas/patologia , Estudos de Casos e Controles , Regulação Neoplásica da Expressão Gênica , Humanos , Mucosa Bucal/enzimologia , Mucosa Bucal/patologia , Neoplasias Bucais/patologia , Ácido Mirístico/metabolismo , Processamento de Proteína Pós-TraducionalRESUMO
PURPOSE: The purposes of this study were to determine the previously unreported prevalence of antral pseudocysts in an exclusively pediatric dental population, characterize their presentation, and investigate potential correlates to the presence of these lesions. METHODS: A tripartite chart review was conducted of 2,000 five- to 21-year-old pediatric dental patients at Yale-New Haven Hospital for the presence of antral pseudocysts identifiable on panoramic radiographs. Demographic, medical, and dental variables potentially influencing their pathogenesis were analyzed via descriptive statistics, odds ratios, and Fisher's exact test. RESULTS: Thirty-six antral pseudocysts were identified in the study sample (1.8 percent). They presented as asymptomatic solitary lesions with a slight male predominance and occurring with greater frequency in individuals with reported allergies. No significant relationship was found between maxillary extractions, orthodontics, obstructive sleep apnea, or history of orofacial trauma and the presence of antral pseudocysts. CONCLUSION: The prevalence of antral pseudocysts in the pediatric dental population falls among the lower end of ranges previously reported in the literature and may reflect their development over time in response to environmental exposures.
Assuntos
Cistos/diagnóstico , Adolescente , Criança , Diagnóstico Diferencial , Feminino , Humanos , Masculino , Maxila , Prevalência , Radiografia Panorâmica , Apneia Obstrutiva do Sono/epidemiologia , Adulto JovemRESUMO
OBJECTIVES: To test whether academic centers (ACs) are more successful than nonacademic centers (NACs) in immunohistochemistry (IHC) external quality assessment challenges in the Canadian Immunohistochemistry Quality Control (CIQC) program. METHODS: Results of 9 CIQC challenges for breast cancer marker (BM) and various non-breast cancer marker (NBM) tests were examined. Success rates were compared between AC/NAC laboratories and those located in small or large cities. Performance was also correlated with annual IHC case volumes. RESULTS: There was no statistically significant difference in performance in any of the comparisons. However, overall performance on BM was significantly better (P < .0001, t test) than on NBM tests regardless of AC/NAC nature or city size. The mean failure rate on NBM was approximately twice that of BM tests. CONCLUSION: Our results suggest that recent emphasis on breast hormone IHC quality assurance has led to improved test quality.