RESUMO
We examined whether gastric acidity would affect the activity of myrosinase, co-delivered with glucoraphanin (GR), to convert GR to sulforaphane (SF). A broccoli seed and sprout extract (BSE) rich in GR and active myrosinase was delivered before and after participants began taking the anti-acid omeprazole, a potent proton pump inhibitor. Gastric acidity appears to attenuate GR bioavailability, as evidenced by more SF and its metabolites being excreted after participants started taking omeprazole. Enteric coating enhanced conversion of GR to SF, perhaps by sparing myrosinase from the acidity of the stomach. There were negligible effects of age, sex, ethnicity, BMI, vegetable consumption, and bowel movement frequency and quality. Greater body mass correlated with reduced conversion efficiency. Changes in the expression of 20 genes in peripheral blood mononuclear cells were evaluated as possible pharmacodynamic indicators. When grouped by their primary functions based on a priori knowledge, expression of genes associated with inflammation decreased non-significantly, and those genes associated with cytoprotection, detoxification and antioxidant functions increased significantly with bioavailability. Using principal components analysis, component loadings of the changes in gene expression confirmed these groupings in a sensitivity analysis.
Assuntos
Brassica , Suplementos Nutricionais , Glucosinolatos/administração & dosagem , Glicosídeo Hidrolases/administração & dosagem , Imidoésteres/administração & dosagem , Isotiocianatos/metabolismo , Omeprazol/administração & dosagem , Extratos Vegetais/administração & dosagem , Inibidores da Bomba de Prótons/administração & dosagem , Plântula , Sementes , Adulto , Idoso , Disponibilidade Biológica , Brassica/química , Suplementos Nutricionais/efeitos adversos , Interações Medicamentosas , Feminino , Ácido Gástrico/metabolismo , Regulação da Expressão Gênica/efeitos dos fármacos , Glucosinolatos/efeitos adversos , Glucosinolatos/isolamento & purificação , Glucosinolatos/metabolismo , Glicosídeo Hidrolases/efeitos adversos , Glicosídeo Hidrolases/metabolismo , Humanos , Concentração de Íons de Hidrogênio , Imidoésteres/efeitos adversos , Imidoésteres/isolamento & purificação , Imidoésteres/metabolismo , Leucócitos Mononucleares/efeitos dos fármacos , Leucócitos Mononucleares/metabolismo , Masculino , Pessoa de Meia-Idade , Omeprazol/efeitos adversos , Oximas , Projetos Piloto , Extratos Vegetais/efeitos adversos , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/metabolismo , Inibidores da Bomba de Prótons/efeitos adversos , Plântula/química , Sementes/química , Sulfóxidos , Adulto JovemRESUMO
Consumers of higher levels of Brassica vegetables, particularly those of the genus Brassica (broccoli, Brussels sprouts and cabbage), reduce their susceptibility to cancer at a variety of organ sites. Brassica vegetables contain high concentrations of glucosinolates that can be hydrolyzed by the plant enzyme, myrosinase, or intestinal microflora to isothiocyanates, potent inducers of cytoprotective enzymes and inhibitors of carcinogenesis. Oral administration of either the isothiocyanate, sulforaphane, or its glucosinolate precursor, glucoraphanin, inhibits mammary carcinogenesis in rats treated with 7,12-dimethylbenz[a]anthracene. In this study, we sought to determine whether sulforaphane exerts a direct chemopreventive action on animal and human mammary tissue. The pharmacokinetics and pharmacodynamics of a single 150 mumol oral dose of sulforaphane were evaluated in the rat mammary gland. We detected sulforaphane metabolites at concentrations known to alter gene expression in cell culture. Elevated cytoprotective NAD(P)H:quinone oxidoreductase (NQO1) and heme oxygenase-1 (HO-1) gene transcripts were measured using quantitative real-time polymerase chain reaction. An observed 3-fold increase in NQO1 enzymatic activity, as well as 4-fold elevated immunostaining of HO-1 in rat mammary epithelium, provides strong evidence of a pronounced pharmacodynamic action of sulforaphane. In a subsequent pilot study, eight healthy women undergoing reduction mammoplasty were given a single dose of a broccoli sprout preparation containing 200 mumol of sulforaphane. Following oral dosing, sulforaphane metabolites were readily measurable in human breast tissue enriched for epithelial cells. These findings provide a strong rationale for evaluating the protective effects of a broccoli sprout preparation in clinical trials of women at risk for breast cancer.