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1.
Cancer Res ; 55(19): 4425-31, 1995 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-7545541

RESUMO

E-, P-, and L-selectin support the adhesion of leukocytes to the vessel wall through the recognition of specific carbohydrate ligands, which often contain sialylated, fucosylated lactosamines such as sialyl Lewis x [sLex; Neu5Ac alpha 2-3Gal beta 1-4(Fuc alpha 1-3)GlcNAc-]. E-selectin expressed by activated endothelium has been shown to support the adhesion of sLex-bearing colon cancer cells. In the present study, we examine the interactions of multiple colon cancer cell lines with all three selectins. Three colon cancer cell lines (LS 180, T84, and COLO 205) bound to recombinant purified E-, P-, and L-selectin. The colon cancer line COLO 320 bound to P- and L-selectin but not E-selectin; conversely, HT-29 cells bound E-selectin but not P- and L-selectin. Caco-2 showed little or no interaction with any of the three selectins. Treatment of the cells with O-sialoglycoprotease from Pasteurella haemolytica, an enzyme that selectively cleaves mucin-type O-linked glycoproteins, reduced binding to purified P- and L-selectin in all cases. In addition, recombinant soluble P- and L-selectin bound to affinity-purified mucins from all adherent tumor cell lines. Of the four tumor cell lines that interacted with E-selectin, O-glycoprotease treatment substantially diminished adhesion of LS 180 and T84, had little effect on COLO 205, and failed to inhibit the binding of HT-29. As predicted by these data, E-selectin showed substantial binding only to mucins purified from LS 180 and T84. These findings suggest that L- and P-selectin interact primarily with mucin-type ligands on colon cancers, whereas E-selectin can recognize both mucin and nonmucin ligands. Binding of the colon cancer lines to purified selectins correlates with their adhesion to activated endothelial cells (E-selectin-dependent), platelets (P-selectin-dependent), and neutrophils (L-selectin-dependent). These differential tumor cell-selectin interactions may influence metastatic spread and may also contribute to the observed variability in host response to tumor progression.


Assuntos
Moléculas de Adesão Celular/fisiologia , Neoplasias do Colo/patologia , Mucinas/fisiologia , Glicoproteínas da Membrana de Plaquetas/fisiologia , Animais , Adesão Celular , Selectina E , Endotélio Vascular/citologia , Humanos , Selectina L , Camundongos , Neutrófilos/citologia , Selectina-P , Adesividade Plaquetária , Células Tumorais Cultivadas
2.
J Immunol ; 161(10): 5445-53, 1998 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-9820520

RESUMO

Secretory component (SC) represents the soluble ectodomain of the polymeric Ig receptor, a membrane protein that transports mucosal Abs across epithelial cells. In the protease-rich environment of the intestine, SC is thought to stabilize the associated IgA by unestablished molecular mechanisms. To address this question, we reconstituted SC-IgA complexes in vitro by incubating dimeric IgA (IgAd) with either recombinant human SC (rSC) or SC isolated from human colostral milk (SCm). Both complexes exhibited an identical degree of covalency when exposed to redox agents, peptidyl disulfide isomerase, and temperature changes. In cross-competition experiments, 50% inhibition of binding to IgAd was achieved at approximately 10 nM SC competitor. Western blot analysis of IgAd digested with intestinal washes indicated that the alpha-chain in IgAd was primarily split into a 40-kDa species, a phenomenon delayed in rSC- or SCm-IgAd complexes. In the same assay, either of the SCs was resistant to degradation only if complexed with IgAd. In contrast, the kappa light chain was not digested at all, suggesting that the F(ab')2 region was left intact. Accordingly, IgAd and SC-IgAd digestion products retained functionality as indicated by Ag reactivity in ELISA. Size exclusion chromatography under native conditions of digested IgAd and rSC-IgAd demonstrates that SC exerts its protective role in secretory IgA by delaying cleavage in the hinge/Fc region of the alpha-chain, not by holding together degraded fragments. The function of integral secretory IgA and F(ab')2 is discussed in terms of mucosal immune defenses.


Assuntos
Imunoglobulina A Secretora/metabolismo , Fragmentos Fab das Imunoglobulinas/metabolismo , Mucosa Intestinal/imunologia , Componente Secretório/fisiologia , Animais , Afinidade de Anticorpos , Antígenos de Bactérias/metabolismo , Sítios de Ligação de Anticorpos , Ligação Competitiva/imunologia , Dimerização , Endopeptidases/metabolismo , Células HeLa , Humanos , Hidrólise , Imunidade nas Mucosas , Imunoglobulina A/metabolismo , Mucosa Intestinal/enzimologia , Cinética , Lipopolissacarídeos/imunologia , Lipopolissacarídeos/metabolismo , Camundongos , Leite/imunologia , Proteínas Recombinantes/imunologia , Proteínas Recombinantes/metabolismo , Componente Secretório/genética , Componente Secretório/metabolismo
3.
J Biol Chem ; 274(44): 31456-62, 1999 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-10531347

RESUMO

We have identified sites for epitope insertion in the murine secretory component (SC) by replacing individual surface-exposed loops in domains I, II, and III with the FLAG sequence (Crottet, P., Peitsch, M. C., Servis, C., and Corthésy, B. (1999) J. Biol. Chem. 274, 31445-31455). We had previously shown that epitope-carrying SC reassociated with dimeric IgA (IgA(d)) can serve as a mucosal delivery vehicle. When analyzing the capacity of SC mutants to associate with IgA(d), we found that all domain II and III mutants bound specifically with immobilized IgA(d), and their affinity for IgA(d) was comparable to that of the wild type protein (IC(50) approximately 1 nM). We conclude that domains II and III in SC are permissive to local mutation and represent convenient sites to antigenize the SC molecule. No mutant bound to monomeric IgA. SC mutants exposing the FLAG at their surface maintained this property once bound to IgA(d), thereby defining regions not required for high affinity binding to IgA(d). Association of IgA(d) with SC mutants carrying a buried FLAG did not expose de novo the epitope, consistent with limited, local changes in the SC structure upon binding. Only wild type and two mutant SCs bound covalently to IgA(d), thus implicating domains II and III in the correct positioning of the reactive cysteine in SC. This establishes that the integrity of murine SC domains II and III is not essential to preserve specific IgA(d) binding but is necessary for covalency to take place. Finally, SC mutants existing in the monomeric and dimeric forms exhibited the same IgA(d) binding capacity as monomeric wild type SC known to bind with a 1:1 stoichiometry.


Assuntos
Imunoglobulina A Secretora/metabolismo , Receptores de Imunoglobulina Polimérica/metabolismo , Animais , Sítios de Ligação , Dimerização , Epitopos , Imunoglobulina A/metabolismo , Camundongos , Oligopeptídeos , Peptídeos , Ligação Proteica , Estrutura Terciária de Proteína , Receptores de Imunoglobulina Polimérica/genética
4.
EMBO J ; 19(24): 6704-12, 2000 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-11118205

RESUMO

We have developed a novel assay to detect the cytosolic localization of protein domains by inserting a short consensus sequence for phosphorylation by protein kinase A. In transfected COS-1 cells, this sequence was labeled efficiently with [(32)P]phosphate only when exposed to the cytosol and not when translocated into the lumen of the endoplasmic reticulum. The phosphorylation state of this sequence can therefore be used to determine the topology of membrane proteins. This assay is sufficiently sensitive to detect even the transient cytosolic exposure of the N-terminal domain of a membrane protein with a reverse signal-anchor sequence. The extent of phosphorylation per newly synthesized polypeptide was shown to reflect the time of exposure to the cytosol, which depends on translation, targeting and translocation of the N-terminus. By altering the length of the N-terminal domain or manipulating the translation rate, it was determined that protein targeting is rapid and requires only a few seconds. The rate of N-terminal translocation was estimated to be approximately 1.6 times the rate of translation.


Assuntos
Proteínas Quinases Dependentes de AMP Cíclico/metabolismo , Retículo Endoplasmático/metabolismo , Proteínas de Membrana/metabolismo , Fosfatos/metabolismo , Sequência de Aminoácidos , Animais , Receptor de Asialoglicoproteína , Células COS , Chlorocebus aethiops , Sequência Consenso , Citosol/metabolismo , Cinética , Proteínas de Membrana/química , Oligopeptídeos/química , Oligopeptídeos/metabolismo , Fosforilação , Transporte Proteico , Receptores de Superfície Celular/química , Receptores de Superfície Celular/metabolismo , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Especificidade por Substrato , Transfecção
5.
Biochem J ; 341 ( Pt 2): 299-306, 1999 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-10393086

RESUMO

Reconstitution of secretory IgA (S-IgA) by the association in vitro of secretory component (SC) and polymeric IgA (pIgA) obtained from hybridomas is a valuable tool in the study of the structure-function relationship in this particular class of antibody. Although dimeric IgA (dIgA) can be obtained and purified from hybridoma clones, SC remains tedious to isolate in sufficient amounts from colostral milk. Several murine models for the study of mucosal immunity are available, which could potentially benefit from the use of cognate IgA antibodies in various molecular forms, including dIgA and S-IgA. We report here on the establishment of two expression systems allowing the production of milligram amounts of pure recombinant murine SC (rmSC) with preserved murine pIgA-binding capability. The first system relies on the use of recombinant vaccinia virus to prompt infected HeLa cells to express the murine SC protein, whereas the second system is based on a stably transfected cell clone exhibiting murine glycosylation. The second source of rmSC will permit the study of the role of its sugar moieties in pathogen-host interactions, and the evaluation of its function in passive protection without risking adverse immune responses. The extensive biochemical characterization conducted in this study demonstrates that rmSC is a dependable and convenient alternative to the natural product, and indicates that the J chain is dispensable in the recognition of pIgA and SC in vitro, whereas it is required for proper pIgA-polymeric Ig receptor interaction in vivo.


Assuntos
Imunoglobulina A , Componente Secretório , Animais , Cromatografia de Afinidade , Clonagem Molecular , Imunidade nas Mucosas , Imunoglobulina A/genética , Imunoglobulina A/imunologia , Camundongos , Plasmídeos , Proteínas Recombinantes/genética , Proteínas Recombinantes/imunologia , Proteínas Recombinantes/isolamento & purificação , Componente Secretório/genética , Componente Secretório/imunologia , Componente Secretório/isolamento & purificação
6.
Bioessays ; 21(7): 558-67, 1999 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-10472183

RESUMO

Sorting of membrane proteins is generally mediated by cytosolic coats, which create a scaffold to form coated buds and vesicles and to selectively concentrate cargo by interacting with cytosolic signals. The classical paradigm is the interaction between clathrin coats and associated adaptor proteins, which cluster receptors with characteristic tyrosine and dileucine motifs during endocytosis. Clathrin in association with different sets of adaptors is found in addition at the trans-Golgi network and endosomes. Sequences similar to internalization signals also direct lysosomal and basolateral sorting, which implicates related clathrinadaptor coats in the respective sorting pathways. This review concentrates on the recognition of sorting signals by clathrin-associated adaptor proteins, an area of significant recent progress due to new methodological and conceptual approaches.


Assuntos
Clatrina/metabolismo , Proteínas de Membrana/metabolismo , Transdução de Sinais , Subunidades alfa do Complexo de Proteínas Adaptadoras , Proteínas Adaptadoras de Transporte Vesicular , Sequência de Aminoácidos , Animais , Humanos , Dados de Sequência Molecular
7.
Glycobiology ; 6(2): 191-208, 1996 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-8727791

RESUMO

Previous studies have shown that the mucin-type polypeptides GlyCAM-1, CD34, and MAdCAM-1 can function as ligands for L-selectin only when they are synthesized by the specialized high-endothelial venules (HEV) of lymph modes. Since sialylation, sulfation, and possibly fucosylation are required for generating recognition, we reasoned that other mucins known to have such components might also bind L-selectin. We show here that soluble mucins secreted by human colon carcinoma cells, as well as those derived from human bronchial mucus can bind to human L-selectin in a calcium-dependent manner. As with Gly-CAM-1 synthesized by lymph node HEV, alpha 2-3 linked sialic acids and sulfation seem to play a critical role in generating this L-selectin binding. In each case, only a subset of the mucin molecules is recognized by L-selectin. Binding is not destroyed by boiling, suggesting that recognition may be based primarily upon carbohydrate structures. Despite this, O-linked oligosaccharide chains released from these ligands by beta-elimination do not show any detectable binding to L-selectin. Following protease treatment of the ligands, binding persists in a subset of the resulting fragments, indicating that specific recognition is determined by certain regions of the original mucins. However, O-linked oligosaccharides released from the subset of non-binding mucin fragments do not show very different size and charge profiles compared to those that do bind. Furthermore, studies with polylactosamine-degrading endoglycosidases suggest that the core structures involved in generating binding can vary among the different ligands. Taken together, these data indicate that a single unique oligosaccharide structure may not be responsible for high-affinity binding. Rather, diverse mucins with sialylated, sulfated, fucosylated lactosamine-type O-linked oligosaccharides can generate high-affinity L-selectin ligands, but only when they present these chains in unique spacing and/or clustered combinations, presumably dictated by the polypeptide backbone.


Assuntos
Selectina L/metabolismo , Mucinas/metabolismo , Amino Açúcares/metabolismo , Brônquios/metabolismo , Sequência de Carboidratos , Linhagem Celular , Neoplasias do Colo/metabolismo , Glicosídeo Hidrolases/metabolismo , Humanos , Hidrólise , Dados de Sequência Molecular , Mucinas/química , Muco/metabolismo , Ácido N-Acetilneuramínico , Oligossacarídeos/química , Oligossacarídeos/metabolismo , Polissacarídeos/metabolismo , Ligação Proteica , Conformação Proteica , Desnaturação Proteica , Ácidos Siálicos/metabolismo
8.
J Biol Chem ; 274(44): 31445-55, 1999 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-10531346

RESUMO

Recombinant secretory immunoglobulin A containing a bacterial epitope in domain I of the secretory component (SC) moiety can serve as a mucosal delivery vehicle triggering both mucosal and systemic responses (Corthésy, B., Kaufmann, M., Phalipon, A., Peitsch, M., Neutra, M. R., and Kraehenbuhl, J.-P. (1996) J. Biol. Chem. 271, 33670-33677). To load recombinant secretory IgA with multiple B and T epitopes and extend its biological functions, we selected, based on molecular modeling, five surface-exposed sites in domains II and III of murine SC. Loops predicted to be exposed at the surface of SC domains were replaced with the DYKDDDDK octapeptide (FLAG). Another two mutants were obtained with the FLAG inserted in between domains II and III or at the carboxyl terminus of SC. As shown by mass spectrometry, internal substitution of the FLAG into four of the mutants induced the formation of disulfide-linked homodimers. Three of the dimers and two of the monomers from SC mutants could be affinity-purified using an antibody to the FLAG, mapping them as candidates for insertion. FLAG-induced dimerization also occurred with the polymeric immunoglobulin receptor (pIgR) and might reflect the so-far nondemonstrated capacity of the receptor to oligomerize. By co-expressing in COS-7 cells and epithelial Caco-2 cells two pIgR constructs tagged at the carboxyl terminus with hexahistidine or FLAG, we provide the strongest evidence reported to date that the pIgR dimerizes noncovalently in the plasma membrane in the absence of polymeric IgA ligand. The implication of this finding is discussed in terms of IgA transport and specific antibody response at mucosal surfaces.


Assuntos
Imunoconjugados/metabolismo , Imunoglobulina A Secretora/metabolismo , Receptores de Imunoglobulina Polimérica/metabolismo , Animais , Células COS , Células CACO-2 , Membrana Celular , Dimerização , Ensaio de Imunoadsorção Enzimática , Epitopos , Humanos , Imunoconjugados/química , Imunoglobulina A Secretora/química , Ligantes , Camundongos , Modelos Moleculares , Oligopeptídeos , Peptídeos , Testes de Precipitina , Engenharia de Proteínas , Receptores de Imunoglobulina Polimérica/química , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
9.
J Biol Chem ; 275(25): 19050-9, 2000 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-10858454

RESUMO

The small GTP-binding protein ADP-ribosylation factor 1 (ARF1) is an essential component of the molecular machinery that catalyzes the formation of membrane-bound transport intermediates. By using an in vitro assay that reproduces recruitment of cytosolic proteins onto purified, high salt-washed Golgi membranes, we have analyzed the role of cAMP-dependent protein kinase A (PKA) on ARF1 incorporation. Addition to this assay of either pure catalytic subunits of PKA (C-PKA) or cAMP increased ARF1 binding. By contrast, ARF1 association was inhibited following C-PKA inactivation with either PKA inhibitory peptide or RIIalpha as well as after cytosol depletion of C-PKA. C-PKA also stimulated recruitment and activation of a recombinant form of human ARF1 in the absence of additional cytosolic components. The binding step could be dissociated from the activation reaction and found to be independent of guanine nucleotides and saturable. This step was stimulated by C-PKA in an ATP-dependent manner. Dephosphorylated Golgi membranes exhibited a decreased ability to recruit ARF1, and this effect was reverted by addition of C-PKA. Following an increase in the intracellular level of cAMP, ARF proteins redistributed from cytosol to the perinuclear Golgi region of intact cells. Collectively, the results show that PKA exerts a key regulatory role in the recruitment of ARF1 onto Golgi membranes. In contrast, PKA modulators did not affect recruitment of beta-COP onto Golgi membranes containing prebound ARF1.


Assuntos
Fator 1 de Ribosilação do ADP/metabolismo , Proteínas Quinases Dependentes de AMP Cíclico/metabolismo , Complexo de Golgi/metabolismo , Animais , Bovinos , Proteína Coatomer/metabolismo , AMP Cíclico/farmacologia , Complexo de Golgi/efeitos dos fármacos , Humanos , Membranas Intracelulares/efeitos dos fármacos , Membranas Intracelulares/metabolismo , Fosforilação , Ligação Proteica , Ratos , Proteínas Recombinantes/metabolismo
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