RESUMO
This study evaluates serum creatine kinase isoenzyme activity in children with osteogenesis imperfecta to determine its usefulness as a biochemical marker during treatment with bisphosphonate. The changes of creatine kinase (CK) isoenzyme activity during and after discontinuation therapy were observed. These results could be useful in addressing over-treatment risk prevention. INTRODUCTION: The brain isoenzyme of creatine kinase (CKbb) is highly expressed in mature osteoclasts during osteoclastogenesis, thus plays an important role in bone resorption. We previously identified high serum CKbb levels in 18 children with osteogenesis imperfect (OI) type 1 treated for 1 year with bisphosphonate (neridronate). In the present study, serum CK isoenzymes were evaluated in the same children with continuous versus discontinued neridronate treatment over a further 2-year follow-up period. METHODS: This study included 18 children with OI type 1, 12 with continued (group A) and 6 with ceased (group B) neridronate treatment. Auxological data, serum biochemical markers of bone metabolism, bone mineral density z-score, and serum total CK and isoenzyme activities were determined in both groups. RESULTS: Serum CKbb was progressively and significantly increased in group A (p < 0.004) but rapidly decreased to undetectable levels in group B. In both groups, the cardiac muscle creatine kinase isoenzyme (CKmb) showed a marked decrease, while serum C-terminal telopeptide (CTx) levels were almost unchanged. CONCLUSIONS: This study provides evidence of the cumulative effect of neridronate administration in increasing serum CKbb levels and the reversible effect after its discontinuation. This approach could be employed for verifying the usefulness of serum CKbb as a biochemical marker in patients receiving prolonged bisphosphonate treatment. Moreover, the decreased serum CKmb levels suggest a systemic effect of these drugs.
Assuntos
Conservadores da Densidade Óssea/uso terapêutico , Creatina Quinase/sangue , Difosfonatos/uso terapêutico , Osteogênese Imperfeita/tratamento farmacológico , Biomarcadores/sangue , Criança , Pré-Escolar , Ensaios Enzimáticos Clínicos/métodos , Monitoramento de Medicamentos/métodos , Feminino , Seguimentos , Humanos , Isoenzimas/sangue , Masculino , Osteogênese Imperfeita/diagnósticoRESUMO
Osteoporosis-pseudoglioma syndrome (OPPG) is a rare autosomal recessive syndrome characterized by juvenile-onset osteoporosis and ocular abnormalities due to a low-density lipoprotein receptor-related protein 5 (LRP5) gene mutation. Treatment with bisphosphonates, particularly with pamidronate and risedronate, has been reported to be of some efficacy in this condition. We report on a patient with OPPG due to an LRP5 gene mutation, who showed an encouraging response after a 36-month period of neridronate therapy. We report a case of a patient treated with bisphosphonates. Bisphosphonates should be administered in OPPG patients as a first-line therapy during early childhood.
Assuntos
Conservadores da Densidade Óssea/uso terapêutico , Difosfonatos/uso terapêutico , Osteogênese Imperfeita/tratamento farmacológico , Adolescente , Humanos , Proteína-5 Relacionada a Receptor de Lipoproteína de Baixa Densidade/genética , Masculino , Mutação , Osteogênese Imperfeita/complicações , Osteogênese Imperfeita/diagnóstico por imagem , Osteogênese Imperfeita/genética , Fraturas por Osteoporose/diagnóstico por imagem , Fraturas por Osteoporose/etiologia , Radiografia , Fraturas da Coluna Vertebral/diagnóstico por imagem , Fraturas da Coluna Vertebral/etiologiaRESUMO
The aim of this study is to evaluate the diagnostic accuracy of vertebral fractures assessment (VFA) in comparison with conventional radiography in identifying vertebral fractures in children and adolescents affected by OI. On 58 patients (33 males, 25 females; age range 1-18 years; 41 children and 17 adolescents) with osteogenesis imperfecta (OI type I, n = 44, OI type III, n = 4; OI type IV, n = 10), lateral spine images by radiographs and by dual-energy X-ray absorptiometry (DXA) were acquired. For vertebral fracture diagnosis, plain radiographs were used as "gold standard" and VFA and morphometric X-ray absorptiometry (MXA) were performed. The visualized vertebrae were 738 (97.9%) by radiographs and 685 (90.9%) by DXA of a total of 754 vertebrae from T4 to L4. VFA and MXA identified, respectively, 129 (74%) and 116 (66%) of the 175 vertebral fractures detected by radiographs. Radiographs identified 36 patients with vertebral fractures, VFA 35 and MXA 41 (6 false positives). On a per vertebra basis, radiographs and VFA had elevated agreement (93.9%; k score 0.81, 95% CI 0.76-0.86), that resulted slightly lower for MXA (90.6%; k score 0.72, 95% CI 0.65-0.78). VFA and MXA demonstrated high sensitivity (95.6 and 94.1 %, respectively) while specificity was 100% for VFA and 90.6% for MXA on a per patient basis; the agreement was excellent for VFA (98.3%; k score 0.96, 95% CI 0.89-1.03) and good for MXA (87.9%; k score 0.73, 95% CI 0.55-0.91). The diagnostic performance parameters resulted better for VFA (sensitivity 95.6%; specificity 100%; PPV 100%; NPV 97.2%), than for MXA (sensitivity 94.1%; specificity 85.4%; PPV 72.7%; NPV 97.2%). The results of our study demonstrate the reliability of VFA for diagnosis of vertebral fractures in children with OI suggesting its use as a more safe and practical alternative to conventional radiography.
Assuntos
Osteogênese Imperfeita/diagnóstico por imagem , Osteogênese Imperfeita/diagnóstico , Fraturas da Coluna Vertebral/diagnóstico por imagem , Fraturas da Coluna Vertebral/diagnóstico , Absorciometria de Fóton , Adolescente , Antropometria , Densidade Óssea , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Vértebras Lombares/diagnóstico por imagem , Masculino , Osteogênese Imperfeita/complicações , Reprodutibilidade dos Testes , Fraturas da Coluna Vertebral/complicações , Coluna Vertebral/diagnóstico por imagem , Vértebras Torácicas/diagnóstico por imagemRESUMO
We describe a 10-year-old boy with glycogen storage disease type Ib (GSD Ib) with neutropenia and neutrophil dysfunction who never suffered from severe recurrent infections. Lymphocyte subpopulations and assay of intracellular cytokines (IL-2, IL-4 and IFN-gamma) showed a pattern of lymphocyte activation suggesting a shift of T(H)1/T(H)2 balance towards a T(H)1 response. This is the first report of GSD Ib without severe recurrent infections in spite of neutropenia and neutrophil dysfunction.
Assuntos
Doença de Depósito de Glicogênio Tipo I/complicações , Doença de Depósito de Glicogênio Tipo I/patologia , Infecções/diagnóstico , Neutropenia/patologia , Neutrófilos/patologia , Criança , Humanos , Interferon gama/biossíntese , Interleucina-2/biossíntese , Interleucina-4/biossíntese , Ativação Linfocitária , Masculino , Recidiva , Células Th1/metabolismo , Células Th2/metabolismoRESUMO
Oral supplementation of 10 mmol/kg/day of D-ribose to a patient with an inherited deficit of adenylosuccinase, severe psychomotor retardation, and epilepsy caused a marked increase in plasma concentration and urinary excretion of urate, while minor changes in succinylpurine levels were observed. D-Ribose administration was accompanied by a slight improvement of behaviour and a progressive reduction of seizure frequency, which increased dramatically upon two attempts to withdraw the drug. Substitution of D-ribose with an equivalent amount of D-glucose did not result in an increase of seizure frequency.
Assuntos
Adenilossuccinato Liase/deficiência , Suplementos Nutricionais , Purinas/biossíntese , Ribose/farmacologia , Adolescente , Feminino , Glucose/metabolismo , Humanos , Exame Neurológico , Ribose/administração & dosagem , Convulsões/prevenção & controle , Transaminases/metabolismoRESUMO
We report on a boy with short stature, mental retardation, seizures, follicular ichthyosis, generalized alopecia, hypohydrosis, enamel dysplasia, photophobia, congenital aganglionic megacolon, inguinal hernia, vertebral, renal and other anomalies, and a normal chromosome constitution. The clinical findings include all the features that dermotrichic and ichthyosis follicularis-alopecia-photophobia (IFAP) syndrome have in common and in addition those that characterize IFAP syndrome (photophobia, recurrent respiratory infections, etc.), those that are present only in dermotrichic syndrome (nail anomalies, hypohydrosis, megacolon, vertebral defects, etc.) and additional ones (enamel dysplasia, renal anomalies, inguinal hernia, etc.). Two maternal uncles were referred as being affected by alopecia and ichthyosis suggesting X-linked recessive transmission. Various hypotheses concerning the relationship between the 2 syndromes and the present case are discussed.
Assuntos
Alopecia/genética , Displasia Ectodérmica/genética , Ictiose Ligada ao Cromossomo X/genética , Pré-Escolar , Displasia Ectodérmica/patologia , Genes Recessivos , Ligação Genética , Humanos , Ictiose Ligada ao Cromossomo X/patologia , Deficiência Intelectual/genética , Luz , Masculino , Linhagem , Síndrome , Cromossomo XRESUMO
The serum tryptophan to large neutral amino acids ratio (Try/LNAA) is considered a reliable marker of tryptophan availability for brain serotonin synthesis. A dysfunction of brain serotonergic activity has been postulated to exist in autistic disorder and supported by recent studies. On this basis, we determined the serum amino acids levels in 40 children with idiopathic infantile autism as well as in 46 control children. A significantly lower serum Try/LNAA ratio was observed in the autistic subjects compared to the normal controls. In 14 autistic children (35%) this ratio was 2 SD below the mean value obtained in the control group. These results suggest that a low brain tryptophan availability due to a low serum Try/LNAA ratio could be one of the possible mechanisms involved in the alteration of serotonergic function in autism.
Assuntos
Aminoácidos/sangue , Transtorno Autístico/sangue , Triptofano/sangue , Adolescente , Sistema Nervoso Central/química , Criança , Feminino , Humanos , Masculino , Serotonina/deficiênciaRESUMO
BACKGROUND: Polyunsaturated fatty acids, as precursors of eicosanoids, are involved in the pathogenesis of oesophageal mucosal damage and healing. AIMS: To evaluate a possible role of polyunsaturated fatty acids in the pathogenesis of gastro-oesophageal reflux, we assayed fatty acids profile of oesophageal mucosal specimens obtained by endoscopy in children without oesophageal disease and children affected by gastro-oesophageal reflux disease. PATIENTS: Eighteen children with normal 24-h oesophageal pH monitoring (GOR- group) and 18 children with gastro-oesophageal reflux disease (GOR+ group, eight with oesophagitis and 10 without), were included in the study. METHODS: Fatty acids were extracted from oesophageal mucosal specimens obtained by endoscopy and assayed by gas chromatography. RESULTS: In the GOR+ group we observed an increased percentage of mucosal polyunsaturated fatty acids, mainly arachidonic and docosohexaenoic acids (p<0.01), without differences between groups with and without oesophagitis. Significant positive correlation was found between reflux index and docosahexaenoic acid (r=0.805; p<0.001). CONCLUSIONS: The results obtained show that the current methods are able to reveal changes between normal and pathological mucosa that could be relevant in the pathogenesis of gastro-oesophageal reflux disease.
Assuntos
Esôfago/metabolismo , Ácidos Graxos Insaturados/metabolismo , Refluxo Gastroesofágico/metabolismo , Ácido Araquidônico/metabolismo , Estudos de Casos e Controles , Criança , Cromatografia Gasosa , Ácidos Docosa-Hexaenoicos/metabolismo , Monitoramento Ambiental , Esofagite Péptica/metabolismo , Esofagoscopia , Feminino , Refluxo Gastroesofágico/etiologia , Humanos , Concentração de Íons de Hidrogênio , Masculino , Mucosa/metabolismoRESUMO
The etiopathogenesis of infantile autism is still unknown. Recently some authors have suggested that food peptides might be able to determine toxic effects at the level of the central nervous system by interacting with neurotransmitters. In fact a worsening of neurological symptoms has been reported in autistic patients after the consumption of milk and wheat. The aim of the present study has been to verify the efficacy of a cow's milk free diet (or other foods which gave a positive result after a skin test) in 36 autistic patients. We also looked for immunological signs of food allergy in autistic patients on a free choice diet. We noticed a marked improvement in the behavioural symptoms of patients after a period of 8 weeks on an elimination diet and we found high levels of IgA antigen specific antibodies for casein, lactalbumin and beta-lactoglobulin and IgG and IgM for casein. The levels of these antibodies were significantly higher than those of a control group which consisted of 20 healthy children. Our results lead us to hypothesise a relationship between food allergy and infantile autism as has already been suggested for other disturbances of the central nervous system.
Assuntos
Transtorno Autístico/etiologia , Hipersensibilidade a Leite/complicações , Adolescente , Animais , Especificidade de Anticorpos , Transtorno Autístico/imunologia , Caseínas/imunologia , Criança , Feminino , Humanos , Imunoglobulinas/análise , Imunoglobulinas/imunologia , Lactalbumina/imunologia , Lactoglobulinas/imunologia , Masculino , Leite/imunologia , Hipersensibilidade a Leite/diagnóstico , Hipersensibilidade a Leite/dietoterapia , Testes CutâneosRESUMO
The efficacy of amoxycillin-clavulanic acid combination in the treatment of urinary tract infections resistant, in vitro, to amoxycillin was studied in 42 children. Of the 24 children with urinary tract infection for the first time, combination therapy, dosing twice daily for 5 days (40 mg/kg.day), cleared the infection in 23 (96%) cases. Relapse occurred in four (17%) cases within 30 days. Of the 18 children who presented with recurrent urinary tract infections therapy, as above, cleared the infection in 16 (89%) cases. In these cases, long-term therapy was performed at a dosage of 20 mg/kg once daily. Tolerance was good; gastro-intestinal disorders in five (12%) cases which regressed by dosing at 8 h rather than 12 h intervals. In conclusion, amoxycillin-clavulanic acid can be considered a first choice treatment of urinary tract infections in children.
Assuntos
Amoxicilina/uso terapêutico , Antibacterianos/uso terapêutico , Ácidos Clavulânicos/uso terapêutico , Infecções Urinárias/tratamento farmacológico , Pré-Escolar , Ácido Clavulânico , Quimioterapia Combinada/uso terapêutico , Feminino , Humanos , MasculinoRESUMO
In the last few years many authors have studied atherosclerosis prevention in paediatrics. In our paper the most recent advances concerning the role of dietary intake in the normal child are described. Human milk and "prudent" diet with low intake of lipids and dietary cholesterol are discussed.
Assuntos
Arteriosclerose/prevenção & controle , Fenômenos Fisiológicos da Nutrição Infantil , Dieta , Fenômenos Fisiológicos da Nutrição do Lactente , Adolescente , Fatores Etários , Arteriosclerose/sangue , Criança , Pré-Escolar , Colesterol/sangue , Colesterol na Dieta/administração & dosagem , Humanos , Lactente , Obesidade/prevenção & controleRESUMO
A group of 210 children, aged one to twelve years (86 males and 124 females) were sent to our Clinic with the diagnosis of microscopic hematuria. Twelve children in which microhematuria wasn't confirmed were immediately excluded; thirty children were excluded because they showed pathologic findings. The remaining 168 children were put under diagnostic examination (hematologic exams, urinoculture, abdomen radiographic, family screening). The cause of microhematuria was identified in 52 of them. The group was selected down to 116 who were then monitored for a period ranging from 1 to 9 years with periodical clinical and biohumoral controls and with diagnostic exams. Biopsies were performed only in 16 cases in which a nephropathy was suspected. Nine cases presented significant histological lesions. Seventy five children were subjected to observation for a period of over one year. After few years hematuria gradually disappeared in twenty six children. After nine years microhematuria had disappeared spontaneously in 39.9% of cases. It remained in core of 33.6% of case, for which no cause could be found. With reference to the full group of 168 children, the following pathologies have been found responsible of microhematuria: a) urinary way infections: 25 cases (14.8%) b) nephropathies: 16 cases (9.5%) c) familiar hematurias: 14 cases (8.3%) d) lithiasis: 9 cases (5.3%) e) alteration of the coagulation system: 3 cases (1.8%) f) renal hypercalciuria without lithiasis: 3 cases (1.8%) Based on these observation, we believe that careful waiting is the best tactic in the cases of monosymptomatic microhematuria.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Hematúria/etiologia , Criança , Pré-Escolar , Feminino , Seguimentos , Humanos , Lactente , Masculino , PrognósticoRESUMO
The AA. studied ninety children affected by microscopic hematuria whose origin was not clear. Periodic controls have been effectuated during a period from one to five years. The hematuria is spontaneously disappeared in the 46,7% of the cases. In sixteen children (17,7%) the hematuria was also present in some relatives, nine children were affected by urinary tract malformations. Only twenty subjects were affected by a significant renal pathology.
Assuntos
Hematúria/etiologia , Biópsia , Criança , Pré-Escolar , Feminino , Glomerulonefrite/patologia , Hematúria/genética , Humanos , Lactente , Glomérulos Renais/patologia , Masculino , Proteinúria/diagnósticoRESUMO
110 children, affected by UTI, were included in this study; 52 of them were treated for 10 days and the remaining 58 for 3 days. Both groups were treated with cotrimoxazole or nitrofurantoin. Urine cultures were repeated soon after the end of therapy and repeated subsequently for a period of 6 months or of 1 year. There is not a great difference between the results obtained with the 10 days treatment and those of the 3 days treatment. An high percentage of relapses occurred in UTI caused by Proteus and Klebsiella.
Assuntos
Nitrofurantoína/uso terapêutico , Sulfametoxazol/uso terapêutico , Trimetoprima/uso terapêutico , Infecções Urinárias/tratamento farmacológico , Criança , Combinação de Medicamentos/uso terapêutico , Enterococcus faecalis , Infecções por Escherichia coli/tratamento farmacológico , Feminino , Humanos , Infecções por Klebsiella/tratamento farmacológico , Masculino , Infecções por Proteus/tratamento farmacológico , Infecções por Pseudomonas/tratamento farmacológico , Infecções Estreptocócicas/tratamento farmacológico , Fatores de Tempo , Combinação Trimetoprima e SulfametoxazolRESUMO
The Authors describe a case of Lyme disease in a 3 year old child who lives in the center of Italy, in the Rome area. In this report it has been underlined the presence of Lyme disease also in central Italy and the importance of early diagnosis and therapy in order to prevent severe complications.
Assuntos
Doença de Lyme/epidemiologia , Pré-Escolar , Feminino , Humanos , Itália/epidemiologia , Doença de Lyme/diagnósticoRESUMO
Osteoporosis-pseudoglioma sydrome (OPPG) is an autosomal recessive disorder with early-onset severe osteoporosis and blindness, caused by biallelic loss-of-function mutations in the low-density lipoprotein receptor-related protein 5 (LRP5) gene. Heterozygous carriers exhibit a milder bone phenotype. Only a few splice mutations in LRP5 have been published. We present clinical and genetic data for four patients with novel LRP5 mutations, three of which affect splicing. Patients were evaluated clinically and by radiography and bone densitometry. Genetic screening of LRP5 was performed on the basis of the clinical diagnosis of OPPG. Splice aberrances were confirmed by cDNA sequencing or exon trapping. The effect of one splice mutation on LRP5 protein function was studied. A novel splice-site mutation c.1584+4A>T abolished the donor splice site of exon 7 and activated a cryptic splice site, which led to an in-frame insertion of 21 amino acids (p.E528_V529ins21). Functional studies revealed severely impaired signal transduction presumably caused by defective intracellular transport of the mutated receptor. Exon trapping was used on two samples to confirm that splice-site mutations c.4112-2A>G and c.1015+1G>T caused splicing-out of exons 20 and 5, respectively. One patient carried a homozygous deletion of exon 4 causing the loss of exons 4 and 5, as demonstrated by cDNA analysis. Our results broaden the spectrum of mutations in LRP5 and provide the first functional data on splice aberrations.
Assuntos
Proteínas Relacionadas a Receptor de LDL/genética , Mutação , Osteogênese Imperfeita/genética , Splicing de RNA , Adolescente , Adulto , Criança , Feminino , Humanos , Proteínas Relacionadas a Receptor de LDL/metabolismo , Proteína-5 Relacionada a Receptor de Lipoproteína de Baixa Densidade , Masculino , Transdução de SinaisRESUMO
The aetiology of thalassemia major-induced osteoporosis is multifactorial. Up to now, bisphosphonates seem to be a promising therapy. Taurine is found in a high concentration in bone cells enhancing bone tissue formation and inhibiting bone loss. Recently we found a decrease taurine plasma level in children affected by osteogenesis imperfecta during neridronate (amino-bisphosphonate) therapy suggesting a possible interaction between pharmacological effect of this drug and taurine availability. On the basis of these results, we performed plasma and urine amino acid (AA) analysis in thalassemia major-induced osteoporosis before and after 12 months of neridronate treatment. Twelve patients, five males and seven females, aged from 20 to 29 years following a hypertransfusion treatment protocol were enrolled in the study. Patients were treated with neridronate infusion every one month (30 mg in 100ml of saline). Plasma and urine specimens for AA analysis, bone mineral density, bone mineral content and vertebral project area were examined at baseline (T0) and after 12 months of treatment (T12). A significant decrease was observed for plasma level and urinary excretion of taurine (T0 vs. T12=p<0.01) whereas bone mineral content and vertebral projection area showed a statistical significant increase (T0 vs. T12=p<0.05). These results and other experimental researches warrant further studies examining the long-term effect of taurine supplementation in association with neridronate treatment.