RESUMO
BACKGROUND: Rhesus and cynomologus macaques are valuable animal models for the study of human immunodeficiency virus (HIV) prevention strategies. However, for such studies focused on the vaginal route of infection, differences in vaginal environment may have deterministic impact on the outcome of such prevention, providing the rationale for this study. METHODS: We tested the vaginal environment of rhesus and cynomolgus macaques longitudinally to characterize the normal microflora based on Nugent scores and pH. This evaluation was extended after colonization of the vaginal space with Lactobacilli in an effort to recreate NHP models representing the healthy human vaginal environment. RESULTS AND CONCLUSION: Nugent scores and pH differed significantly between species, although data from both species were suggestive of stable bacterial vaginosis. Colonization with Lactobacilli was successful in both species leading to lower Nugent score and pH, although rhesus macaques appeared better able to sustain Lactobacillus spp over time.
Assuntos
Lactobacillus/fisiologia , Macaca fascicularis/microbiologia , Macaca mulatta/microbiologia , Vagina/microbiologia , Animais , Modelos Animais de Doenças , Feminino , Infecções por HIV/prevenção & controle , Humanos , Valores de ReferênciaRESUMO
On postmortem examination, 2 geriatric captive male squirrel monkeys (Saimiri sciureus) were found to have pituitary masses that were unassociated with previous experimental manipulation. Both animals were euthanized due to apparently unrelated clinical reasons. Histopathology and immunohistochemical staining classified these tumors as thyrotrophic and corticotrophic pituitary adenomas. These cases represent the first reports of this tumor type in squirrel monkeys.